Modelling and analysis of real-time PCR data


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Documentation for package ‘qpcR’ version 1.3-4

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AICc Akaike's second-order corrected Information Criterion
akaike.weights Calculation of Akaike weights/relative likelihoods/delta-AICs
b3 The nonlinear models implemented in qpcR
b4 The nonlinear models implemented in qpcR
b5 The nonlinear models implemented in qpcR
b6 The nonlinear models implemented in qpcR
b7 The nonlinear models implemented in qpcR
baseline Baselining and refitting six-parameter sigmoidal models
batchstat Concatenating or calculating statistics on a 'pcrbatch'
batsch1 qPCR dilution experiments from Batsch et al. (2008)
batsch2 qPCR dilution experiments from Batsch et al. (2008)
batsch3 qPCR dilution experiments from Batsch et al. (2008)
batsch4 qPCR dilution experiments from Batsch et al. (2008)
batsch5 qPCR dilution experiments from Batsch et al. (2008)
boggy qPCR dilution experiments from Boggy et al. (2010)
calib Calculation of qPCR efficiency by dilution curve analysis
calib2 Calculation of qPCR efficiency by dilution curve bootstrapping
cbind.na Create or combine data of unequal size by Rows or Columns
curvemean Building a model which averages a batch of qPCR curves
Cy0 Cy0 alternative to threshold cycles as in Guescini et al. (2008)
data.frame.na Create or combine data of unequal size by Rows or Columns
dyemelt Melting curves of a 4-plex qPCR with different fluorescence dyes
eff The amplification efficiency curve of a fitted object
efficiency Calculation of qPCR efficiency and other important qPCR parameters
efficiency.modlist Calculation of qPCR efficiency and other important qPCR parameters
evidence Evidence ratio for model comparison with AIC or AICc
expcomp Comparison of all sigmodal models within the exponential region
expfit Calculation of PCR efficiency by fitting an exponential model
expGrowth The nonlinear models implemented in qpcR
fitchisq The chi-square goodness-of-fit
getPar Batch calculation of qPCR efficiencies/threshold cycles with simple output, especially tailored to high-throughput data
guescini1 qPCR dilution experiments Set #1 from Guescini et al. (2008)
guescini2 qPCR dilution experiments Set #2 from Guescini et al. (2008)
is.outlier Outlier summary for objects of class 'pcrfit, 'modlist' or 'replist'
KOD (K)inetic (O)utlier (D)etection according to Bar et al. (2003)
l3 The nonlinear models implemented in qpcR
l4 The nonlinear models implemented in qpcR
l5 The nonlinear models implemented in qpcR
l6 The nonlinear models implemented in qpcR
l7 The nonlinear models implemented in qpcR
LOF.test Formal Lack-Of-Fit test of a nonlinear model against a one-way ANOVA model
LR Calculation of likelihood ratios for nested models
mak2 The nonlinear models implemented in qpcR
mak3 The nonlinear models implemented in qpcR
makXpar Parameters that can be changed to tweak the mak2/mak3 methods
maxRatio The maxRatio method as in Shain et al. (2008)
meanlist Amalgamation of single data models into an averaged model
meltcurve Melting curve analysis with (iterative) Tm identification and peak area calculation/cutoff
midpoint Calculation of the 'midpoint' region according to Peirson et al. (2003)
modlist Create nonlinear models from a dataframe and coerce them into a list
mselect Sigmoidal model selection by different criteria
neill.test Neill's lack-of-fit test when replicates are lacking
outlier Calculation of qPCR outlier cycles
pcrbatch Batch calculation of qPCR efficiency and other qPCR parameters
pcrboot Bootstrapping and jackknifing qPCR data
pcrfit Function for qPCR model fitting
pcrGOF Summarize measures for the goodness-of-fit
pcrimport Advanced qPCR data import function
pcrimport2 Simple qPCR data import function (i.e. from text files or clipboard)
pcropt1 Combinatorial elimination of plateau and ground phase cycles
pcropt2 Elimination of qPCR cycles with low/high impact on fitted parameters
pcrsim Simulation of sigmoidal qPCR data with goodness-of-fit analysis
plot.pcrfit Plotting qPCR data with fitted curves/confidence bands/error bars
predict.pcrfit Value prediction from a fitted qPCR model
PRESS Allen's PRESS (Prediction Sum-Of-Squares) statistic, aka P-square
propagate General error analysis function using different methods
qpcR.news Display news and changes of qpcR package versions
ratiobatch Calculation of ratios in a batch format for multiple genes/samples
ratiocalc Calculation of ratios from qPCR runs with/without reference data
rbind.na Create or combine data of unequal size by Rows or Columns
rep2mod Converts a 'replist' back to a 'modlist'
replist Amalgamation of single data models into a model containing replicates
reps qPCR dilution experiments Set #1 from the package author
reps2 qPCR dilution experiments Set #2 from the package author
reps3 qPCR dilution experiments Set #3 from the package author
resplot A residuals bar-plot with colour-coded bars
resVar Residual variance of a fitted model
RMSE Root-mean-squared-error of a fitted model
Rsq R-square value of a fitted model
Rsq.ad Adjusted R-square value of a fitted model
RSS Residual sum-of-squares of a fitted model
rutledge qPCR dilution experiments from Rutledge et al. (2004)
S27 qPCR data of the S27a transcript in 15 testicular biopsies
sisti1 qPCR dilution experiments Set #1 from Sisti et al. (2010)
sisti2 qPCR dilution experiments Set #2 from Sisti et al. (2010)
sliwin Calculation of PCR efficiency by the window-of-linearity method
SOD (S)igmoidal (O)utlier (D)etection using first/second derivative maxima
update.pcrfit Updating and refitting a qPCR model