CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/001.mrc Output diagnostic file name Micrographs/001.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/001.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2250.000 8510.000 Mean, RMS : 4396.886 225.8583 TITLE 1: emi2mrc: 001.emi Sun Jan 19 12:38:35 2014 TITLE 2: kV 300, Mag 78000x, Def 28550, Spot 4, Int 55.16%, Obj 88.00%, Dif 73.67% TITLE 3: X 427.261, Y 214.948, Z -104.060, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.03440 13400.00 8000.00 0.00 -0.03415 13600.00 8000.00 0.00 -0.03352 13800.00 8000.00 0.00 -0.03277 14000.00 8000.00 0.00 -0.03202 14200.00 8000.00 0.00 -0.03133 14400.00 8000.00 0.00 -0.03073 14600.00 8000.00 0.00 -0.03018 14800.00 8000.00 0.00 -0.02960 15000.00 8000.00 0.00 -0.02896 15200.00 8000.00 0.00 -0.02823 15400.00 8000.00 0.00 -0.02738 15600.00 8000.00 0.00 -0.02644 15800.00 8000.00 0.00 -0.02541 16000.00 8000.00 0.00 -0.02432 16200.00 8000.00 0.00 -0.02317 16400.00 8000.00 0.00 -0.02198 16600.00 8000.00 0.00 -0.02072 16800.00 8000.00 0.00 -0.01935 17000.00 8000.00 0.00 -0.01786 17200.00 8000.00 0.00 -0.01626 17400.00 8000.00 0.00 -0.01457 17600.00 8000.00 0.00 -0.01288 17800.00 8000.00 0.00 -0.01121 18000.00 8000.00 0.00 -0.00956 18200.00 8000.00 0.00 -0.00788 18400.00 8000.00 0.00 -0.00612 18600.00 8000.00 0.00 -0.00425 18800.00 8000.00 0.00 -0.00232 19000.00 8000.00 0.00 -0.00039 19200.00 8000.00 0.00 0.00145 19400.00 8000.00 0.00 0.00316 19600.00 8000.00 0.00 0.00479 19800.00 8000.00 0.00 0.00641 20000.00 8000.00 0.00 0.00815 20200.00 8000.00 0.00 0.01007 20400.00 8000.00 0.00 0.01219 20600.00 8000.00 0.00 0.01448 20800.00 8000.00 0.00 0.01686 21000.00 8000.00 0.00 0.01930 21200.00 8000.00 0.00 0.02175 21400.00 8000.00 0.00 0.02424 21600.00 8000.00 0.00 0.02676 21800.00 8000.00 0.00 0.02929 22000.00 8000.00 0.00 0.03184 22200.00 8000.00 0.00 0.03445 22400.00 8000.00 0.00 0.03718 22600.00 8000.00 0.00 0.04014 22800.00 8000.00 0.00 0.04335 23000.00 8000.00 0.00 0.04669 23200.00 8000.00 0.00 0.04995 23400.00 8000.00 0.00 0.05283 23600.00 8000.00 0.00 0.05507 23800.00 8000.00 0.00 0.05660 24000.00 8000.00 0.00 0.05750 24200.00 8000.00 0.00 0.05790 24400.00 8000.00 0.00 0.05798 24200.00 8200.00 0.00 0.05826 24400.00 8200.00 0.00 0.05833 24200.00 8400.00 0.00 0.05868 24400.00 8400.00 0.00 0.05874 24000.00 8600.00 0.00 0.05886 24200.00 8600.00 0.00 0.05924 24400.00 8600.00 0.00 0.05929 24000.00 8800.00 0.00 0.05955 24200.00 8800.00 0.00 0.05992 24400.00 8800.00 0.00 0.05994 24000.00 9000.00 0.00 0.06029 24200.00 9000.00 0.00 0.06063 24400.00 9000.00 0.00 0.06063 24000.00 9200.00 0.00 0.06104 24200.00 9200.00 0.00 0.06135 24000.00 9400.00 0.00 0.06176 24200.00 9400.00 0.00 0.06204 24000.00 9600.00 0.00 0.06243 24200.00 9600.00 0.00 0.06268 24000.00 9800.00 0.00 0.06312 24200.00 9800.00 0.00 0.06334 24000.00 10000.00 0.00 0.06385 24200.00 10000.00 0.00 0.06405 24000.00 10200.00 0.00 0.06460 24200.00 10200.00 0.00 0.06477 24000.00 10400.00 0.00 0.06535 24200.00 10400.00 0.00 0.06551 24000.00 10600.00 0.00 0.06612 24200.00 10600.00 0.00 0.06625 24000.00 10800.00 0.00 0.06690 24200.00 10800.00 0.00 0.06701 23800.00 11000.00 0.00 0.06710 24000.00 11000.00 0.00 0.06775 24200.00 11000.00 0.00 0.06784 23800.00 11200.00 0.00 0.06809 24000.00 11200.00 0.00 0.06871 24200.00 11200.00 0.00 0.06877 23800.00 11400.00 0.00 0.06911 24000.00 11400.00 0.00 0.06971 24200.00 11400.00 0.00 0.06974 23800.00 11600.00 0.00 0.07010 24000.00 11600.00 0.00 0.07068 24200.00 11600.00 0.00 0.07069 23800.00 11800.00 0.00 0.07106 24000.00 11800.00 0.00 0.07161 23800.00 12000.00 0.00 0.07196 24000.00 12000.00 0.00 0.07250 23800.00 12200.00 0.00 0.07287 24000.00 12200.00 0.00 0.07340 23800.00 12400.00 0.00 0.07389 24000.00 12400.00 0.00 0.07441 23800.00 12600.00 0.00 0.07501 24000.00 12600.00 0.00 0.07552 23800.00 12800.00 0.00 0.07614 24000.00 12800.00 0.00 0.07663 23800.00 13000.00 0.00 0.07727 24000.00 13000.00 0.00 0.07774 23800.00 13200.00 0.00 0.07842 24000.00 13200.00 0.00 0.07887 23800.00 13400.00 0.00 0.07961 24000.00 13400.00 0.00 0.08003 23800.00 13600.00 0.00 0.08095 24000.00 13600.00 0.00 0.08135 23800.00 13800.00 0.00 0.08247 24000.00 13800.00 0.00 0.08284 23600.00 14000.00 0.00 0.08295 23800.00 14000.00 0.00 0.08407 24000.00 14000.00 0.00 0.08441 23600.00 14200.00 0.00 0.08457 23800.00 14200.00 0.00 0.08567 24000.00 14200.00 0.00 0.08598 23600.00 14400.00 0.00 0.08613 23800.00 14400.00 0.00 0.08721 24000.00 14400.00 0.00 0.08750 23600.00 14600.00 0.00 0.08761 23800.00 14600.00 0.00 0.08868 24000.00 14600.00 0.00 0.08896 23600.00 14800.00 0.00 0.08910 23800.00 14800.00 0.00 0.09016 24000.00 14800.00 0.00 0.09045 23600.00 15000.00 0.00 0.09071 23800.00 15000.00 0.00 0.09178 24000.00 15000.00 0.00 0.09207 23600.00 15200.00 0.00 0.09243 23800.00 15200.00 0.00 0.09352 24000.00 15200.00 0.00 0.09382 23600.00 15400.00 0.00 0.09424 23800.00 15400.00 0.00 0.09535 24000.00 15400.00 0.00 0.09567 23600.00 15600.00 0.00 0.09612 23800.00 15600.00 0.00 0.09726 24000.00 15600.00 0.00 0.09760 23600.00 15800.00 0.00 0.09805 23800.00 15800.00 0.00 0.09922 24000.00 15800.00 0.00 0.09956 23600.00 16000.00 0.00 0.10007 23800.00 16000.00 0.00 0.10124 24000.00 16000.00 0.00 0.10157 23600.00 16200.00 0.00 0.10224 23800.00 16200.00 0.00 0.10341 24000.00 16200.00 0.00 0.10371 23600.00 16400.00 0.00 0.10455 23800.00 16400.00 0.00 0.10569 24000.00 16400.00 0.00 0.10596 23600.00 16600.00 0.00 0.10688 23800.00 16600.00 0.00 0.10799 24000.00 16600.00 0.00 0.10820 23600.00 16800.00 0.00 0.10919 23800.00 16800.00 0.00 0.11026 24000.00 16800.00 0.00 0.11041 23600.00 17000.00 0.00 0.11146 23800.00 17000.00 0.00 0.11248 24000.00 17000.00 0.00 0.11255 23600.00 17200.00 0.00 0.11367 23800.00 17200.00 0.00 0.11463 23600.00 17400.00 0.00 0.11594 23800.00 17400.00 0.00 0.11682 23600.00 17600.00 0.00 0.11829 23800.00 17600.00 0.00 0.11911 23600.00 17800.00 0.00 0.12062 23800.00 17800.00 0.00 0.12139 23600.00 18000.00 0.00 0.12283 23800.00 18000.00 0.00 0.12356 23600.00 18200.00 0.00 0.12494 23800.00 18200.00 0.00 0.12562 23600.00 18400.00 0.00 0.12694 23800.00 18400.00 0.00 0.12759 23600.00 18600.00 0.00 0.12900 23800.00 18600.00 0.00 0.12963 23600.00 18800.00 0.00 0.13136 23800.00 18800.00 0.00 0.13196 23400.00 19000.00 0.00 0.13213 23600.00 19000.00 0.00 0.13398 23800.00 19000.00 0.00 0.13454 23400.00 19200.00 0.00 0.13487 23600.00 19200.00 0.00 0.13670 23800.00 19200.00 0.00 0.13722 23400.00 19400.00 0.00 0.13767 23600.00 19400.00 0.00 0.13949 23800.00 19400.00 0.00 0.13996 23400.00 19600.00 0.00 0.14045 23600.00 19600.00 0.00 0.14224 23800.00 19600.00 0.00 0.14264 23400.00 19800.00 0.00 0.14318 23600.00 19800.00 0.00 0.14493 23800.00 19800.00 0.00 0.14525 23400.00 20000.00 0.00 0.14603 23600.00 20000.00 0.00 0.14772 23800.00 20000.00 0.00 0.14794 23400.00 20200.00 0.00 0.14902 23600.00 20200.00 0.00 0.15063 23800.00 20200.00 0.00 0.15073 23400.00 20400.00 0.00 0.15196 23600.00 20400.00 0.00 0.15346 23400.00 20600.00 0.00 0.15477 23600.00 20600.00 0.00 0.15616 23400.00 20800.00 0.00 0.15743 23600.00 20800.00 0.00 0.15871 23400.00 21000.00 0.00 0.15996 23600.00 21000.00 0.00 0.16113 23400.00 21200.00 0.00 0.16259 23600.00 21200.00 0.00 0.16365 23400.00 21400.00 0.00 0.16549 23600.00 21400.00 0.00 0.16644 23400.00 21600.00 0.00 0.16850 23600.00 21600.00 0.00 0.16933 23400.00 21800.00 0.00 0.17144 23600.00 21800.00 0.00 0.17212 23400.00 22000.00 0.00 0.17422 23600.00 22000.00 0.00 0.17474 23400.00 22200.00 0.00 0.17677 23600.00 22200.00 0.00 0.17712 23400.00 22400.00 0.00 0.17931 23600.00 22400.00 0.00 0.17946 23200.00 22600.00 0.00 0.18012 23400.00 22600.00 0.00 0.18220 23200.00 22800.00 0.00 0.18344 23400.00 22800.00 0.00 0.18525 23200.00 23000.00 0.00 0.18646 23400.00 23000.00 0.00 0.18789 23200.00 23200.00 0.00 0.18875 23400.00 23200.00 0.00 0.18969 23200.00 23400.00 0.00 0.18979 23400.00 23400.00 0.00 0.19014 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 23303.81 23413.89 31.66 0.19040 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/001.ctf NX, NY, NZ: 360 360 1 MODE : real