CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/004.mrc Output diagnostic file name Micrographs/004.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/004.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2254.000 7580.000 Mean, RMS : 4398.142 229.2360 TITLE 1: emi2mrc: 004.emi Sun Jan 19 12:47:25 2014 TITLE 2: kV 300, Mag 78000x, Def 29700, Spot 4, Int 55.14%, Obj 88.04%, Dif 73.67% TITLE 3: X 331.653, Y 147.527, Z -112.287, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.08562 35400.00 8000.00 0.00 -0.08452 35600.00 8000.00 0.00 -0.08292 35800.00 8000.00 0.00 -0.08108 36000.00 8000.00 0.00 -0.07925 36200.00 8000.00 0.00 -0.07751 36400.00 8000.00 0.00 -0.07556 36600.00 8000.00 0.00 -0.07338 36800.00 8000.00 0.00 -0.07155 37000.00 8000.00 0.00 -0.07032 37200.00 8000.00 0.00 -0.06940 37400.00 8000.00 0.00 -0.06863 37600.00 8000.00 0.00 -0.06818 37800.00 8000.00 0.00 -0.06809 38400.00 18400.00 0.00 -0.06807 38600.00 18400.00 0.00 -0.06795 37800.00 18600.00 0.00 -0.06783 38000.00 18600.00 0.00 -0.06776 38200.00 18600.00 0.00 -0.06772 38400.00 18600.00 0.00 -0.06756 38600.00 18600.00 0.00 -0.06746 37800.00 18800.00 0.00 -0.06731 38000.00 18800.00 0.00 -0.06726 38200.00 18800.00 0.00 -0.06722 38400.00 18800.00 0.00 -0.06707 38600.00 18800.00 0.00 -0.06698 37800.00 19000.00 0.00 -0.06678 38000.00 19000.00 0.00 -0.06673 38200.00 19000.00 0.00 -0.06669 38400.00 19000.00 0.00 -0.06654 38600.00 19000.00 0.00 -0.06646 37600.00 19200.00 0.00 -0.06641 37800.00 19200.00 0.00 -0.06615 38000.00 19200.00 0.00 -0.06610 38200.00 19200.00 0.00 -0.06605 38400.00 19200.00 0.00 -0.06589 38600.00 19200.00 0.00 -0.06580 37600.00 19400.00 0.00 -0.06568 37800.00 19400.00 0.00 -0.06542 38000.00 19400.00 0.00 -0.06535 38200.00 19400.00 0.00 -0.06529 38400.00 19400.00 0.00 -0.06512 38600.00 19400.00 0.00 -0.06502 37600.00 19600.00 0.00 -0.06486 37800.00 19600.00 0.00 -0.06459 38000.00 19600.00 0.00 -0.06450 38200.00 19600.00 0.00 -0.06441 38400.00 19600.00 0.00 -0.06423 38600.00 19600.00 0.00 -0.06412 37600.00 19800.00 0.00 -0.06401 37800.00 19800.00 0.00 -0.06371 38000.00 19800.00 0.00 -0.06359 38200.00 19800.00 0.00 -0.06348 38400.00 19800.00 0.00 -0.06328 38600.00 19800.00 0.00 -0.06318 37600.00 20000.00 0.00 -0.06318 37800.00 20000.00 0.00 -0.06285 38000.00 20000.00 0.00 -0.06270 38200.00 20000.00 0.00 -0.06257 38400.00 20000.00 0.00 -0.06237 38600.00 20000.00 0.00 -0.06230 37800.00 20200.00 0.00 -0.06201 38000.00 20200.00 0.00 -0.06184 38200.00 20200.00 0.00 -0.06170 38400.00 20200.00 0.00 -0.06154 38600.00 20200.00 0.00 -0.06152 37600.00 20400.00 0.00 -0.06152 37800.00 20400.00 0.00 -0.06115 38000.00 20400.00 0.00 -0.06098 38200.00 20400.00 0.00 -0.06087 38400.00 20400.00 0.00 -0.06075 37600.00 20600.00 0.00 -0.06062 37800.00 20600.00 0.00 -0.06026 38000.00 20600.00 0.00 -0.06010 38200.00 20600.00 0.00 -0.06003 38400.00 20600.00 0.00 -0.05997 37600.00 20800.00 0.00 -0.05966 37800.00 20800.00 0.00 -0.05931 38000.00 20800.00 0.00 -0.05919 38200.00 20800.00 0.00 -0.05916 37600.00 21000.00 0.00 -0.05866 37800.00 21000.00 0.00 -0.05834 38000.00 21000.00 0.00 -0.05824 37600.00 21200.00 0.00 -0.05771 37800.00 21200.00 0.00 -0.05741 38000.00 21200.00 0.00 -0.05735 37600.00 21400.00 0.00 -0.05686 37800.00 21400.00 0.00 -0.05659 38000.00 21400.00 0.00 -0.05655 37600.00 21600.00 0.00 -0.05611 37800.00 21600.00 0.00 -0.05583 38000.00 21600.00 0.00 -0.05581 37600.00 21800.00 0.00 -0.05537 37800.00 21800.00 0.00 -0.05508 38000.00 21800.00 0.00 -0.05505 37600.00 22000.00 0.00 -0.05458 37800.00 22000.00 0.00 -0.05427 38000.00 22000.00 0.00 -0.05422 37600.00 22200.00 0.00 -0.05368 37800.00 22200.00 0.00 -0.05335 38000.00 22200.00 0.00 -0.05329 37600.00 22400.00 0.00 -0.05267 37800.00 22400.00 0.00 -0.05234 38000.00 22400.00 0.00 -0.05229 37400.00 22600.00 0.00 -0.05229 37600.00 22600.00 0.00 -0.05164 37800.00 22600.00 0.00 -0.05133 38000.00 22600.00 0.00 -0.05131 37400.00 22800.00 0.00 -0.05125 37600.00 22800.00 0.00 -0.05063 37800.00 22800.00 0.00 -0.05037 37400.00 23000.00 0.00 -0.05025 37600.00 23000.00 0.00 -0.04969 37800.00 23000.00 0.00 -0.04948 37400.00 23200.00 0.00 -0.04934 37600.00 23200.00 0.00 -0.04884 37800.00 23200.00 0.00 -0.04869 37400.00 23400.00 0.00 -0.04850 37600.00 23400.00 0.00 -0.04806 37800.00 23400.00 0.00 -0.04797 37400.00 23600.00 0.00 -0.04774 37600.00 23600.00 0.00 -0.04733 37800.00 23600.00 0.00 -0.04727 37400.00 23800.00 0.00 -0.04703 37600.00 23800.00 0.00 -0.04663 37800.00 23800.00 0.00 -0.04655 37400.00 24000.00 0.00 -0.04630 37600.00 24000.00 0.00 -0.04588 37800.00 24000.00 0.00 -0.04577 37400.00 24200.00 0.00 -0.04549 37600.00 24200.00 0.00 -0.04501 37800.00 24200.00 0.00 -0.04487 37400.00 24400.00 0.00 -0.04453 37600.00 24400.00 0.00 -0.04401 37800.00 24400.00 0.00 -0.04383 37400.00 24600.00 0.00 -0.04342 37600.00 24600.00 0.00 -0.04287 37800.00 24600.00 0.00 -0.04267 37400.00 24800.00 0.00 -0.04215 37600.00 24800.00 0.00 -0.04159 37800.00 24800.00 0.00 -0.04140 37400.00 25000.00 0.00 -0.04080 37600.00 25000.00 0.00 -0.04025 37800.00 25000.00 0.00 -0.04009 37400.00 25200.00 0.00 -0.03944 37600.00 25200.00 0.00 -0.03893 37800.00 25200.00 0.00 -0.03881 37400.00 25400.00 0.00 -0.03810 37600.00 25400.00 0.00 -0.03762 37800.00 25400.00 0.00 -0.03755 37400.00 25600.00 0.00 -0.03678 37600.00 25600.00 0.00 -0.03634 37800.00 25600.00 0.00 -0.03631 37400.00 25800.00 0.00 -0.03549 37600.00 25800.00 0.00 -0.03507 37200.00 26000.00 0.00 -0.03501 37400.00 26000.00 0.00 -0.03417 37600.00 26000.00 0.00 -0.03377 37200.00 26200.00 0.00 -0.03366 37400.00 26200.00 0.00 -0.03282 37600.00 26200.00 0.00 -0.03243 37200.00 26400.00 0.00 -0.03230 37400.00 26400.00 0.00 -0.03147 37600.00 26400.00 0.00 -0.03109 37200.00 26600.00 0.00 -0.03096 37400.00 26600.00 0.00 -0.03013 37600.00 26600.00 0.00 -0.02977 37200.00 26800.00 0.00 -0.02962 37400.00 26800.00 0.00 -0.02880 37600.00 26800.00 0.00 -0.02847 37200.00 27000.00 0.00 -0.02832 37400.00 27000.00 0.00 -0.02750 37600.00 27000.00 0.00 -0.02719 37200.00 27200.00 0.00 -0.02701 37400.00 27200.00 0.00 -0.02620 37600.00 27200.00 0.00 -0.02589 37200.00 27400.00 0.00 -0.02567 37400.00 27400.00 0.00 -0.02484 37600.00 27400.00 0.00 -0.02455 37200.00 27600.00 0.00 -0.02427 37400.00 27600.00 0.00 -0.02344 37600.00 27600.00 0.00 -0.02315 37200.00 27800.00 0.00 -0.02283 37400.00 27800.00 0.00 -0.02200 37600.00 27800.00 0.00 -0.02172 37200.00 28000.00 0.00 -0.02135 37400.00 28000.00 0.00 -0.02052 37600.00 28000.00 0.00 -0.02025 37200.00 28200.00 0.00 -0.01985 37400.00 28200.00 0.00 -0.01903 37600.00 28200.00 0.00 -0.01880 37200.00 28400.00 0.00 -0.01835 37400.00 28400.00 0.00 -0.01757 37600.00 28400.00 0.00 -0.01737 37200.00 28600.00 0.00 -0.01685 37400.00 28600.00 0.00 -0.01610 37600.00 28600.00 0.00 -0.01595 37200.00 28800.00 0.00 -0.01530 37400.00 28800.00 0.00 -0.01459 37600.00 28800.00 0.00 -0.01449 37200.00 29000.00 0.00 -0.01369 37400.00 29000.00 0.00 -0.01302 37600.00 29000.00 0.00 -0.01296 37200.00 29200.00 0.00 -0.01198 37400.00 29200.00 0.00 -0.01135 37600.00 29200.00 0.00 -0.01133 37200.00 29400.00 0.00 -0.01021 37400.00 29400.00 0.00 -0.00962 37200.00 29600.00 0.00 -0.00844 37400.00 29600.00 0.00 -0.00789 37200.00 29800.00 0.00 -0.00672 37400.00 29800.00 0.00 -0.00623 37200.00 30000.00 0.00 -0.00507 37400.00 30000.00 0.00 -0.00463 37000.00 30200.00 0.00 -0.00461 37200.00 30200.00 0.00 -0.00348 37400.00 30200.00 0.00 -0.00308 37000.00 30400.00 0.00 -0.00298 37200.00 30400.00 0.00 -0.00188 37400.00 30400.00 0.00 -0.00152 37000.00 30600.00 0.00 -0.00129 37200.00 30600.00 0.00 -0.00021 37400.00 30600.00 0.00 0.00013 37000.00 30800.00 0.00 0.00049 37200.00 30800.00 0.00 0.00156 37400.00 30800.00 0.00 0.00189 37000.00 31000.00 0.00 0.00235 37200.00 31000.00 0.00 0.00342 37400.00 31000.00 0.00 0.00373 37000.00 31200.00 0.00 0.00426 37200.00 31200.00 0.00 0.00532 37400.00 31200.00 0.00 0.00561 37000.00 31400.00 0.00 0.00616 37200.00 31400.00 0.00 0.00719 37400.00 31400.00 0.00 0.00744 37000.00 31600.00 0.00 0.00800 37200.00 31600.00 0.00 0.00899 37400.00 31600.00 0.00 0.00920 37000.00 31800.00 0.00 0.00974 37200.00 31800.00 0.00 0.01069 37400.00 31800.00 0.00 0.01085 37000.00 32000.00 0.00 0.01136 37200.00 32000.00 0.00 0.01227 37400.00 32000.00 0.00 0.01237 37000.00 32200.00 0.00 0.01291 37200.00 32200.00 0.00 0.01379 37400.00 32200.00 0.00 0.01384 37000.00 32400.00 0.00 0.01446 37200.00 32400.00 0.00 0.01530 37400.00 32400.00 0.00 0.01530 37000.00 32600.00 0.00 0.01599 37200.00 32600.00 0.00 0.01680 37000.00 32800.00 0.00 0.01758 37200.00 32800.00 0.00 0.01836 37000.00 33000.00 0.00 0.01930 37200.00 33000.00 0.00 0.02005 37000.00 33200.00 0.00 0.02107 37200.00 33200.00 0.00 0.02178 37000.00 33400.00 0.00 0.02283 37200.00 33400.00 0.00 0.02349 37000.00 33600.00 0.00 0.02464 37200.00 33600.00 0.00 0.02524 37000.00 33800.00 0.00 0.02650 37200.00 33800.00 0.00 0.02702 37000.00 34000.00 0.00 0.02830 37200.00 34000.00 0.00 0.02874 37000.00 34200.00 0.00 0.03007 37200.00 34200.00 0.00 0.03041 36800.00 34400.00 0.00 0.03056 37000.00 34400.00 0.00 0.03188 37200.00 34400.00 0.00 0.03212 36800.00 34600.00 0.00 0.03242 37000.00 34600.00 0.00 0.03366 37200.00 34600.00 0.00 0.03381 36800.00 34800.00 0.00 0.03415 37000.00 34800.00 0.00 0.03532 37200.00 34800.00 0.00 0.03538 36800.00 35000.00 0.00 0.03584 37000.00 35000.00 0.00 0.03694 36800.00 35200.00 0.00 0.03764 37000.00 35200.00 0.00 0.03867 36800.00 35400.00 0.00 0.03959 37000.00 35400.00 0.00 0.04052 36800.00 35600.00 0.00 0.04159 37000.00 35600.00 0.00 0.04240 36800.00 35800.00 0.00 0.04355 37000.00 35800.00 0.00 0.04421 36800.00 36000.00 0.00 0.04543 37000.00 36000.00 0.00 0.04588 36800.00 36200.00 0.00 0.04705 37000.00 36200.00 0.00 0.04725 36800.00 36400.00 0.00 0.04816 36800.00 36600.00 0.00 0.04867 36800.00 36800.00 0.00 0.04867 36600.00 37000.00 30.00 0.04875 36600.00 37000.00 35.00 0.04887 36600.00 37000.00 40.00 0.04899 36600.00 37000.00 45.00 0.04908 36600.00 37000.00 50.00 0.04915 36600.00 37000.00 55.00 0.04918 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 36611.96 36985.94 56.82 0.04919 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/004.ctf NX, NY, NZ: 360 360 1 MODE : real