CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/006.mrc Output diagnostic file name Micrographs/006.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/006.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2130.000 7862.000 Mean, RMS : 4363.635 219.9208 TITLE 1: emi2mrc: 006.emi Sun Jan 19 12:51:04 2014 TITLE 2: kV 300, Mag 78000x, Def 27890, Spot 4, Int 55.14%, Obj 88.07%, Dif 73.67% TITLE 3: X 325.279, Y 142.431, Z -112.287, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.02132 8200.00 8000.00 0.00 -0.02129 8400.00 8000.00 0.00 -0.02114 8600.00 8000.00 0.00 -0.02109 10800.00 8000.00 0.00 -0.02095 11000.00 8000.00 0.00 -0.02065 11200.00 8000.00 0.00 -0.02039 11400.00 8000.00 0.00 -0.02024 11600.00 8000.00 0.00 -0.02018 11800.00 8000.00 0.00 -0.02014 12000.00 8000.00 0.00 -0.02001 12200.00 8000.00 0.00 -0.01974 12400.00 8000.00 0.00 -0.01934 12600.00 8000.00 0.00 -0.01886 12800.00 8000.00 0.00 -0.01835 13000.00 8000.00 0.00 -0.01782 13200.00 8000.00 0.00 -0.01726 13400.00 8000.00 0.00 -0.01665 13600.00 8000.00 0.00 -0.01595 13800.00 8000.00 0.00 -0.01518 14000.00 8000.00 0.00 -0.01433 14200.00 8000.00 0.00 -0.01339 14400.00 8000.00 0.00 -0.01234 14600.00 8000.00 0.00 -0.01119 14800.00 8000.00 0.00 -0.00994 15000.00 8000.00 0.00 -0.00861 15200.00 8000.00 0.00 -0.00723 15400.00 8000.00 0.00 -0.00581 15600.00 8000.00 0.00 -0.00434 15800.00 8000.00 0.00 -0.00281 16000.00 8000.00 0.00 -0.00120 16200.00 8000.00 0.00 0.00051 16400.00 8000.00 0.00 0.00234 16600.00 8000.00 0.00 0.00430 16800.00 8000.00 0.00 0.00632 17000.00 8000.00 0.00 0.00833 17200.00 8000.00 0.00 0.01026 17400.00 8000.00 0.00 0.01209 17600.00 8000.00 0.00 0.01388 17800.00 8000.00 0.00 0.01571 18000.00 8000.00 0.00 0.01768 18200.00 8000.00 0.00 0.01982 18400.00 8000.00 0.00 0.02206 18600.00 8000.00 0.00 0.02433 18800.00 8000.00 0.00 0.02659 19000.00 8000.00 0.00 0.02884 19200.00 8000.00 0.00 0.03116 19400.00 8000.00 0.00 0.03360 19600.00 8000.00 0.00 0.03616 19800.00 8000.00 0.00 0.03879 20000.00 8000.00 0.00 0.04142 20200.00 8000.00 0.00 0.04408 20400.00 8000.00 0.00 0.04685 20600.00 8000.00 0.00 0.04988 20800.00 8000.00 0.00 0.05331 21000.00 8000.00 0.00 0.05708 21200.00 8000.00 0.00 0.06100 21400.00 8000.00 0.00 0.06471 21600.00 8000.00 0.00 0.06780 21800.00 8000.00 0.00 0.06997 22000.00 8000.00 0.00 0.07113 22200.00 8000.00 0.00 0.07142 22000.00 8200.00 0.00 0.07177 22200.00 8200.00 0.00 0.07205 22000.00 8400.00 0.00 0.07237 22200.00 8400.00 0.00 0.07264 22000.00 8600.00 0.00 0.07309 22200.00 8600.00 0.00 0.07334 22000.00 8800.00 0.00 0.07393 22200.00 8800.00 0.00 0.07417 22000.00 9000.00 0.00 0.07485 22200.00 9000.00 0.00 0.07508 22000.00 9200.00 0.00 0.07580 22200.00 9200.00 0.00 0.07603 22000.00 9400.00 0.00 0.07668 22200.00 9400.00 0.00 0.07691 22000.00 9600.00 0.00 0.07747 22200.00 9600.00 0.00 0.07770 22000.00 9800.00 0.00 0.07825 22200.00 9800.00 0.00 0.07849 22000.00 10000.00 0.00 0.07913 22200.00 10000.00 0.00 0.07937 22000.00 10200.00 0.00 0.08010 22200.00 10200.00 0.00 0.08033 22000.00 10400.00 0.00 0.08119 22200.00 10400.00 0.00 0.08142 22000.00 10600.00 0.00 0.08240 22200.00 10600.00 0.00 0.08260 22000.00 10800.00 0.00 0.08362 22200.00 10800.00 0.00 0.08378 22000.00 11000.00 0.00 0.08486 22200.00 11000.00 0.00 0.08496 21800.00 11200.00 0.00 0.08499 22000.00 11200.00 0.00 0.08614 22200.00 11200.00 0.00 0.08619 21800.00 11400.00 0.00 0.08631 22000.00 11400.00 0.00 0.08742 22200.00 11400.00 0.00 0.08743 21800.00 11600.00 0.00 0.08762 22000.00 11600.00 0.00 0.08871 21800.00 11800.00 0.00 0.08898 22000.00 11800.00 0.00 0.09004 21800.00 12000.00 0.00 0.09038 22000.00 12000.00 0.00 0.09143 21800.00 12200.00 0.00 0.09187 22000.00 12200.00 0.00 0.09289 21800.00 12400.00 0.00 0.09351 22000.00 12400.00 0.00 0.09449 21800.00 12600.00 0.00 0.09526 22000.00 12600.00 0.00 0.09620 21800.00 12800.00 0.00 0.09702 22000.00 12800.00 0.00 0.09789 21800.00 13000.00 0.00 0.09874 22000.00 13000.00 0.00 0.09954 21800.00 13200.00 0.00 0.10039 22000.00 13200.00 0.00 0.10112 21800.00 13400.00 0.00 0.10201 22000.00 13400.00 0.00 0.10268 21800.00 13600.00 0.00 0.10370 22000.00 13600.00 0.00 0.10433 21800.00 13800.00 0.00 0.10553 22000.00 13800.00 0.00 0.10614 21800.00 14000.00 0.00 0.10741 22000.00 14000.00 0.00 0.10800 21800.00 14200.00 0.00 0.10931 22000.00 14200.00 0.00 0.10990 21800.00 14400.00 0.00 0.11122 22000.00 14400.00 0.00 0.11183 21800.00 14600.00 0.00 0.11316 22000.00 14600.00 0.00 0.11379 21800.00 14800.00 0.00 0.11526 22000.00 14800.00 0.00 0.11591 21800.00 15000.00 0.00 0.11767 22000.00 15000.00 0.00 0.11832 21800.00 15200.00 0.00 0.12030 22000.00 15200.00 0.00 0.12092 21600.00 15400.00 0.00 0.12093 21800.00 15400.00 0.00 0.12299 22000.00 15400.00 0.00 0.12358 21600.00 15600.00 0.00 0.12363 21800.00 15600.00 0.00 0.12567 22000.00 15600.00 0.00 0.12621 21800.00 15800.00 0.00 0.12820 22000.00 15800.00 0.00 0.12870 21800.00 16000.00 0.00 0.13062 22000.00 16000.00 0.00 0.13107 21600.00 16200.00 0.00 0.13115 21800.00 16200.00 0.00 0.13312 22000.00 16200.00 0.00 0.13353 21600.00 16400.00 0.00 0.13382 21800.00 16400.00 0.00 0.13578 22000.00 16400.00 0.00 0.13616 21600.00 16600.00 0.00 0.13660 21800.00 16600.00 0.00 0.13856 22000.00 16600.00 0.00 0.13892 21600.00 16800.00 0.00 0.13952 21800.00 16800.00 0.00 0.14148 22000.00 16800.00 0.00 0.14182 21600.00 17000.00 0.00 0.14251 21800.00 17000.00 0.00 0.14448 22000.00 17000.00 0.00 0.14479 21600.00 17200.00 0.00 0.14549 21800.00 17200.00 0.00 0.14744 22000.00 17200.00 0.00 0.14772 21600.00 17400.00 0.00 0.14856 21800.00 17400.00 0.00 0.15049 22000.00 17400.00 0.00 0.15071 21600.00 17600.00 0.00 0.15182 21800.00 17600.00 0.00 0.15371 22000.00 17600.00 0.00 0.15387 21600.00 17800.00 0.00 0.15515 21800.00 17800.00 0.00 0.15699 22000.00 17800.00 0.00 0.15706 21600.00 18000.00 0.00 0.15849 21800.00 18000.00 0.00 0.16027 21600.00 18200.00 0.00 0.16185 21800.00 18200.00 0.00 0.16357 21600.00 18400.00 0.00 0.16516 21800.00 18400.00 0.00 0.16681 21600.00 18600.00 0.00 0.16850 21800.00 18600.00 0.00 0.17006 21600.00 18800.00 0.00 0.17208 21800.00 18800.00 0.00 0.17354 21600.00 19000.00 0.00 0.17587 21800.00 19000.00 0.00 0.17722 21600.00 19200.00 0.00 0.17975 21800.00 19200.00 0.00 0.18096 21600.00 19400.00 0.00 0.18370 21800.00 19400.00 0.00 0.18478 21600.00 19600.00 0.00 0.18764 21800.00 19600.00 0.00 0.18857 21600.00 19800.00 0.00 0.19154 21800.00 19800.00 0.00 0.19229 21400.00 20000.00 0.00 0.19271 21600.00 20000.00 0.00 0.19561 21800.00 20000.00 0.00 0.19616 21400.00 20200.00 0.00 0.19717 21600.00 20200.00 0.00 0.19989 21800.00 20200.00 0.00 0.20018 21400.00 20400.00 0.00 0.20168 21600.00 20400.00 0.00 0.20417 21800.00 20400.00 0.00 0.20418 21400.00 20600.00 0.00 0.20621 21600.00 20600.00 0.00 0.20842 21400.00 20800.00 0.00 0.21061 21600.00 20800.00 0.00 0.21248 21400.00 21000.00 0.00 0.21451 21600.00 21000.00 0.00 0.21600 21400.00 21200.00 0.00 0.21790 21600.00 21200.00 0.00 0.21896 21400.00 21400.00 0.00 0.22076 21600.00 21400.00 0.00 0.22128 21400.00 21600.00 0.00 0.22245 21400.00 21600.00 5.00 0.22253 21400.00 21600.00 10.00 0.22259 21400.00 21600.00 15.00 0.22264 21400.00 21600.00 20.00 0.22266 21400.00 21600.00 25.00 0.22266 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 21400.00 21600.00 22.62 0.22266 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/006.ctf NX, NY, NZ: 360 360 1 MODE : real