CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/007.mrc Output diagnostic file name Micrographs/007.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/007.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2362.000 8328.000 Mean, RMS : 4394.148 226.9589 TITLE 1: emi2mrc: 007.emi Sun Jan 19 12:46:27 2014 TITLE 2: kV 300, Mag 78000x, Def 29700, Spot 4, Int 55.14%, Obj 88.04%, Dif 73.67% TITLE 3: X 333.544, Y 145.581, Z -112.287, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 119 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.07548 31000.00 8000.00 0.00 -0.07419 31200.00 8000.00 0.00 -0.07259 31400.00 8000.00 0.00 -0.07086 31600.00 8000.00 0.00 -0.06941 31800.00 8000.00 0.00 -0.06798 32000.00 8000.00 0.00 -0.06628 32200.00 8000.00 0.00 -0.06463 32400.00 8000.00 0.00 -0.06296 32600.00 8000.00 0.00 -0.06080 32800.00 8000.00 0.00 -0.05847 33000.00 8000.00 0.00 -0.05643 33200.00 8000.00 0.00 -0.05429 33400.00 8000.00 0.00 -0.05186 33600.00 8000.00 0.00 -0.04964 33800.00 8000.00 0.00 -0.04763 34000.00 8000.00 0.00 -0.04553 34200.00 8000.00 0.00 -0.04366 34400.00 8000.00 0.00 -0.04250 34600.00 8000.00 0.00 -0.04188 34800.00 8000.00 0.00 -0.04151 35000.00 8000.00 0.00 -0.04144 34600.00 16800.00 0.00 -0.04143 34800.00 16800.00 0.00 -0.04099 35000.00 16800.00 0.00 -0.04085 34600.00 17000.00 0.00 -0.04072 34800.00 17000.00 0.00 -0.04028 35000.00 17000.00 0.00 -0.04016 34600.00 17200.00 0.00 -0.03994 34800.00 17200.00 0.00 -0.03951 35000.00 17200.00 0.00 -0.03940 34600.00 17400.00 0.00 -0.03908 34800.00 17400.00 0.00 -0.03867 35000.00 17400.00 0.00 -0.03858 34600.00 17600.00 0.00 -0.03818 34800.00 17600.00 0.00 -0.03778 35000.00 17600.00 0.00 -0.03772 34600.00 17800.00 0.00 -0.03723 34800.00 17800.00 0.00 -0.03686 35000.00 17800.00 0.00 -0.03683 34600.00 18000.00 0.00 -0.03629 34800.00 18000.00 0.00 -0.03595 35000.00 18000.00 0.00 -0.03595 34600.00 18200.00 0.00 -0.03541 34800.00 18200.00 0.00 -0.03510 34600.00 18400.00 0.00 -0.03460 34800.00 18400.00 0.00 -0.03431 34600.00 18600.00 0.00 -0.03384 34800.00 18600.00 0.00 -0.03356 34600.00 18800.00 0.00 -0.03308 34800.00 18800.00 0.00 -0.03280 34600.00 19000.00 0.00 -0.03222 34800.00 19000.00 0.00 -0.03194 34400.00 19200.00 0.00 -0.03194 34600.00 19200.00 0.00 -0.03121 34800.00 19200.00 0.00 -0.03094 34400.00 19400.00 0.00 -0.03078 34600.00 19400.00 0.00 -0.03006 34800.00 19400.00 0.00 -0.02981 34400.00 19600.00 0.00 -0.02956 34600.00 19600.00 0.00 -0.02885 34800.00 19600.00 0.00 -0.02863 34400.00 19800.00 0.00 -0.02836 34600.00 19800.00 0.00 -0.02767 34800.00 19800.00 0.00 -0.02747 34400.00 20000.00 0.00 -0.02726 34600.00 20000.00 0.00 -0.02659 34800.00 20000.00 0.00 -0.02641 34400.00 20200.00 0.00 -0.02623 34600.00 20200.00 0.00 -0.02557 34800.00 20200.00 0.00 -0.02542 34400.00 20400.00 0.00 -0.02520 34600.00 20400.00 0.00 -0.02456 34800.00 20400.00 0.00 -0.02444 34400.00 20600.00 0.00 -0.02413 34600.00 20600.00 0.00 -0.02351 34800.00 20600.00 0.00 -0.02343 34400.00 20800.00 0.00 -0.02302 34600.00 20800.00 0.00 -0.02245 34800.00 20800.00 0.00 -0.02241 34400.00 21000.00 0.00 -0.02195 34600.00 21000.00 0.00 -0.02142 34400.00 21200.00 0.00 -0.02098 34600.00 21200.00 0.00 -0.02050 34400.00 21400.00 0.00 -0.02013 34600.00 21400.00 0.00 -0.01969 34400.00 21600.00 0.00 -0.01932 34600.00 21600.00 0.00 -0.01890 34400.00 21800.00 0.00 -0.01844 34600.00 21800.00 0.00 -0.01803 34400.00 22000.00 0.00 -0.01742 34600.00 22000.00 0.00 -0.01701 34400.00 22200.00 0.00 -0.01625 34600.00 22200.00 0.00 -0.01585 34400.00 22400.00 0.00 -0.01500 34600.00 22400.00 0.00 -0.01461 34400.00 22600.00 0.00 -0.01379 34600.00 22600.00 0.00 -0.01341 34400.00 22800.00 0.00 -0.01264 34600.00 22800.00 0.00 -0.01227 34400.00 23000.00 0.00 -0.01154 34600.00 23000.00 0.00 -0.01117 34400.00 23200.00 0.00 -0.01041 34600.00 23200.00 0.00 -0.01003 34400.00 23400.00 0.00 -0.00916 34600.00 23400.00 0.00 -0.00876 34400.00 23600.00 0.00 -0.00777 34600.00 23600.00 0.00 -0.00733 34400.00 23800.00 0.00 -0.00624 34600.00 23800.00 0.00 -0.00577 34400.00 24000.00 0.00 -0.00463 34600.00 24000.00 0.00 -0.00414 34400.00 24200.00 0.00 -0.00299 34600.00 24200.00 0.00 -0.00249 34400.00 24400.00 0.00 -0.00133 34600.00 24400.00 0.00 -0.00083 34400.00 24600.00 0.00 0.00036 34600.00 24600.00 0.00 0.00087 34400.00 24800.00 0.00 0.00214 34600.00 24800.00 0.00 0.00263 34200.00 25000.00 0.00 0.00272 34400.00 25000.00 0.00 0.00402 34600.00 25000.00 0.00 0.00449 34200.00 25200.00 0.00 0.00468 34400.00 25200.00 0.00 0.00597 34600.00 25200.00 0.00 0.00642 34200.00 25400.00 0.00 0.00667 34400.00 25400.00 0.00 0.00793 34600.00 25400.00 0.00 0.00834 34200.00 25600.00 0.00 0.00857 34400.00 25600.00 0.00 0.00980 34600.00 25600.00 0.00 0.01016 34200.00 25800.00 0.00 0.01031 34400.00 25800.00 0.00 0.01150 34600.00 25800.00 0.00 0.01181 34200.00 26000.00 0.00 0.01185 34400.00 26000.00 0.00 0.01300 34600.00 26000.00 0.00 0.01325 34400.00 26200.00 0.00 0.01433 34600.00 26200.00 0.00 0.01453 34400.00 26400.00 0.00 0.01560 34600.00 26400.00 0.00 0.01575 34200.00 26600.00 0.00 0.01585 34400.00 26600.00 0.00 0.01688 34600.00 26600.00 0.00 0.01699 34200.00 26800.00 0.00 0.01723 34400.00 26800.00 0.00 0.01823 34600.00 26800.00 0.00 0.01829 34200.00 27000.00 0.00 0.01870 34400.00 27000.00 0.00 0.01965 34600.00 27000.00 0.00 0.01967 34200.00 27200.00 0.00 0.02022 34400.00 27200.00 0.00 0.02113 34200.00 27400.00 0.00 0.02177 34400.00 27400.00 0.00 0.02265 34200.00 27600.00 0.00 0.02337 34400.00 27600.00 0.00 0.02424 34200.00 27800.00 0.00 0.02508 34400.00 27800.00 0.00 0.02594 34200.00 28000.00 0.00 0.02690 34400.00 28000.00 0.00 0.02777 34200.00 28200.00 0.00 0.02883 34400.00 28200.00 0.00 0.02970 34200.00 28400.00 0.00 0.03084 34400.00 28400.00 0.00 0.03169 34200.00 28600.00 0.00 0.03279 34400.00 28600.00 0.00 0.03362 34200.00 28800.00 0.00 0.03464 34400.00 28800.00 0.00 0.03542 34200.00 29000.00 0.00 0.03640 34400.00 29000.00 0.00 0.03713 34200.00 29200.00 0.00 0.03809 34400.00 29200.00 0.00 0.03875 34200.00 29400.00 0.00 0.03975 34400.00 29400.00 0.00 0.04035 34200.00 29600.00 0.00 0.04152 34400.00 29600.00 0.00 0.04207 34200.00 29800.00 0.00 0.04341 34400.00 29800.00 0.00 0.04391 34200.00 30000.00 0.00 0.04534 34400.00 30000.00 0.00 0.04579 34000.00 30200.00 0.00 0.04594 34200.00 30200.00 0.00 0.04734 34400.00 30200.00 0.00 0.04774 34000.00 30400.00 0.00 0.04801 34200.00 30400.00 0.00 0.04935 34400.00 30400.00 0.00 0.04969 34000.00 30600.00 0.00 0.04991 34200.00 30600.00 0.00 0.05119 34400.00 30600.00 0.00 0.05147 34000.00 30800.00 0.00 0.05171 34200.00 30800.00 0.00 0.05293 34400.00 30800.00 0.00 0.05316 34000.00 31000.00 0.00 0.05349 34200.00 31000.00 0.00 0.05467 34400.00 31000.00 0.00 0.05485 34000.00 31200.00 0.00 0.05520 34200.00 31200.00 0.00 0.05635 34400.00 31200.00 0.00 0.05647 34000.00 31400.00 0.00 0.05693 34200.00 31400.00 0.00 0.05803 34400.00 31400.00 0.00 0.05808 34000.00 31600.00 0.00 0.05888 34200.00 31600.00 0.00 0.05991 34000.00 31800.00 0.00 0.06087 34200.00 31800.00 0.00 0.06183 34000.00 32000.00 0.00 0.06271 34200.00 32000.00 0.00 0.06356 34000.00 32200.00 0.00 0.06451 34200.00 32200.00 0.00 0.06523 34000.00 32400.00 0.00 0.06620 34200.00 32400.00 0.00 0.06679 34000.00 32600.00 0.00 0.06758 34200.00 32600.00 0.00 0.06802 34000.00 32800.00 0.00 0.06884 34200.00 32800.00 0.00 0.06909 34000.00 33000.00 0.00 0.07026 34200.00 33000.00 0.00 0.07030 34000.00 33200.00 0.00 0.07158 34000.00 33400.00 0.00 0.07264 33800.00 33600.00 0.00 0.07273 34000.00 33600.00 0.00 0.07361 33800.00 33800.00 0.00 0.07373 34000.00 33800.00 0.00 0.07436 34000.00 34000.00 0.00 0.07448 33800.00 34200.00 25.00 0.07454 33800.00 34200.00 30.00 0.07464 33800.00 34200.00 35.00 0.07472 33800.00 34200.00 40.00 0.07476 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 33829.16 34112.09 44.59 0.07483 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/007.ctf NX, NY, NZ: 360 360 1 MODE : real