CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/009.mrc Output diagnostic file name Micrographs/009.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/009.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2356.000 8376.000 Mean, RMS : 4333.307 217.6559 TITLE 1: emi2mrc: 009.emi Sun Jan 19 12:48:35 2014 TITLE 2: kV 300, Mag 78000x, Def 30190, Spot 4, Int 55.14%, Obj 88.09%, Dif 73.67% TITLE 3: X 329.463, Y 145.422, Z -112.287, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.00645 8200.00 8000.00 0.00 -0.00612 8400.00 8000.00 0.00 -0.00582 8600.00 8000.00 0.00 -0.00557 8800.00 8000.00 0.00 -0.00536 9000.00 8000.00 0.00 -0.00511 9200.00 8000.00 0.00 -0.00473 9400.00 8000.00 0.00 -0.00422 9600.00 8000.00 0.00 -0.00358 9800.00 8000.00 0.00 -0.00290 10000.00 8000.00 0.00 -0.00226 10200.00 8000.00 0.00 -0.00170 10400.00 8000.00 0.00 -0.00117 10600.00 8000.00 0.00 -0.00062 10800.00 8000.00 0.00 0.00000 11000.00 8000.00 0.00 0.00071 11200.00 8000.00 0.00 0.00151 11400.00 8000.00 0.00 0.00238 11600.00 8000.00 0.00 0.00329 11800.00 8000.00 0.00 0.00423 12000.00 8000.00 0.00 0.00520 12200.00 8000.00 0.00 0.00620 12400.00 8000.00 0.00 0.00726 12600.00 8000.00 0.00 0.00844 12800.00 8000.00 0.00 0.00981 13000.00 8000.00 0.00 0.01144 13200.00 8000.00 0.00 0.01340 13400.00 8000.00 0.00 0.01567 13600.00 8000.00 0.00 0.01820 13800.00 8000.00 0.00 0.02091 14000.00 8000.00 0.00 0.02371 14200.00 8000.00 0.00 0.02653 14400.00 8000.00 0.00 0.02937 14600.00 8000.00 0.00 0.03226 14800.00 8000.00 0.00 0.03523 15000.00 8000.00 0.00 0.03833 15200.00 8000.00 0.00 0.04159 15400.00 8000.00 0.00 0.04496 15600.00 8000.00 0.00 0.04837 15800.00 8000.00 0.00 0.05174 16000.00 8000.00 0.00 0.05499 16200.00 8000.00 0.00 0.05817 16400.00 8000.00 0.00 0.06140 16600.00 8000.00 0.00 0.06478 16800.00 8000.00 0.00 0.06844 17000.00 8000.00 0.00 0.07244 17200.00 8000.00 0.00 0.07666 17400.00 8000.00 0.00 0.08093 17600.00 8000.00 0.00 0.08503 17800.00 8000.00 0.00 0.08868 18000.00 8000.00 0.00 0.09164 18200.00 8000.00 0.00 0.09383 18400.00 8000.00 0.00 0.09526 18600.00 8000.00 0.00 0.09607 18800.00 8000.00 0.00 0.09649 19000.00 8000.00 0.00 0.09672 19200.00 8000.00 0.00 0.09680 18600.00 8200.00 0.00 0.09758 18800.00 8200.00 0.00 0.09802 19000.00 8200.00 0.00 0.09826 19200.00 8200.00 0.00 0.09836 18600.00 8400.00 0.00 0.09911 18800.00 8400.00 0.00 0.09955 19000.00 8400.00 0.00 0.09979 19200.00 8400.00 0.00 0.09988 18400.00 8600.00 0.00 0.09997 18600.00 8600.00 0.00 0.10078 18800.00 8600.00 0.00 0.10120 19000.00 8600.00 0.00 0.10142 19200.00 8600.00 0.00 0.10149 18400.00 8800.00 0.00 0.10174 18600.00 8800.00 0.00 0.10252 18800.00 8800.00 0.00 0.10292 19000.00 8800.00 0.00 0.10310 19200.00 8800.00 0.00 0.10313 18400.00 9000.00 0.00 0.10347 18600.00 9000.00 0.00 0.10422 18800.00 9000.00 0.00 0.10457 19000.00 9000.00 0.00 0.10471 18400.00 9200.00 0.00 0.10512 18600.00 9200.00 0.00 0.10582 18800.00 9200.00 0.00 0.10614 19000.00 9200.00 0.00 0.10623 18400.00 9400.00 0.00 0.10668 18600.00 9400.00 0.00 0.10735 18800.00 9400.00 0.00 0.10762 19000.00 9400.00 0.00 0.10767 18400.00 9600.00 0.00 0.10821 18600.00 9600.00 0.00 0.10886 18800.00 9600.00 0.00 0.10910 19000.00 9600.00 0.00 0.10911 18400.00 9800.00 0.00 0.10994 18600.00 9800.00 0.00 0.11056 18800.00 9800.00 0.00 0.11077 18400.00 10000.00 0.00 0.11193 18600.00 10000.00 0.00 0.11253 18800.00 10000.00 0.00 0.11272 18200.00 10200.00 0.00 0.11282 18400.00 10200.00 0.00 0.11406 18600.00 10200.00 0.00 0.11464 18800.00 10200.00 0.00 0.11481 18200.00 10400.00 0.00 0.11498 18400.00 10400.00 0.00 0.11621 18600.00 10400.00 0.00 0.11678 18800.00 10400.00 0.00 0.11694 18200.00 10600.00 0.00 0.11707 18400.00 10600.00 0.00 0.11831 18600.00 10600.00 0.00 0.11888 18800.00 10600.00 0.00 0.11903 18200.00 10800.00 0.00 0.11906 18400.00 10800.00 0.00 0.12031 18600.00 10800.00 0.00 0.12088 18800.00 10800.00 0.00 0.12102 18200.00 11000.00 0.00 0.12111 18400.00 11000.00 0.00 0.12237 18600.00 11000.00 0.00 0.12294 18800.00 11000.00 0.00 0.12306 18200.00 11200.00 0.00 0.12341 18400.00 11200.00 0.00 0.12468 18600.00 11200.00 0.00 0.12523 18800.00 11200.00 0.00 0.12534 18200.00 11400.00 0.00 0.12591 18400.00 11400.00 0.00 0.12718 18600.00 11400.00 0.00 0.12773 18800.00 11400.00 0.00 0.12781 18200.00 11600.00 0.00 0.12854 18400.00 11600.00 0.00 0.12981 18600.00 11600.00 0.00 0.13035 18800.00 11600.00 0.00 0.13038 18200.00 11800.00 0.00 0.13126 18400.00 11800.00 0.00 0.13254 18600.00 11800.00 0.00 0.13305 18200.00 12000.00 0.00 0.13399 18400.00 12000.00 0.00 0.13526 18600.00 12000.00 0.00 0.13573 18200.00 12200.00 0.00 0.13678 18400.00 12200.00 0.00 0.13803 18600.00 12200.00 0.00 0.13842 18200.00 12400.00 0.00 0.13982 18400.00 12400.00 0.00 0.14103 18600.00 12400.00 0.00 0.14135 18200.00 12600.00 0.00 0.14314 18400.00 12600.00 0.00 0.14428 18600.00 12600.00 0.00 0.14452 18200.00 12800.00 0.00 0.14658 18400.00 12800.00 0.00 0.14766 18600.00 12800.00 0.00 0.14780 18000.00 13000.00 0.00 0.14803 18200.00 13000.00 0.00 0.15011 18400.00 13000.00 0.00 0.15111 18600.00 13000.00 0.00 0.15114 18000.00 13200.00 0.00 0.15161 18200.00 13200.00 0.00 0.15363 18400.00 13200.00 0.00 0.15452 18000.00 13400.00 0.00 0.15512 18200.00 13400.00 0.00 0.15705 18400.00 13400.00 0.00 0.15784 18000.00 13600.00 0.00 0.15875 18200.00 13600.00 0.00 0.16059 18400.00 13600.00 0.00 0.16128 18000.00 13800.00 0.00 0.16261 18200.00 13800.00 0.00 0.16437 18400.00 13800.00 0.00 0.16496 18000.00 14000.00 0.00 0.16655 18200.00 14000.00 0.00 0.16823 18400.00 14000.00 0.00 0.16873 18000.00 14200.00 0.00 0.17051 18200.00 14200.00 0.00 0.17212 18400.00 14200.00 0.00 0.17253 18000.00 14400.00 0.00 0.17447 18200.00 14400.00 0.00 0.17601 18400.00 14400.00 0.00 0.17634 18000.00 14600.00 0.00 0.17834 18200.00 14600.00 0.00 0.17980 18400.00 14600.00 0.00 0.18003 18000.00 14800.00 0.00 0.18223 18200.00 14800.00 0.00 0.18360 18400.00 14800.00 0.00 0.18373 17800.00 15000.00 0.00 0.18381 18000.00 15000.00 0.00 0.18634 18200.00 15000.00 0.00 0.18763 18400.00 15000.00 0.00 0.18764 17800.00 15200.00 0.00 0.18811 18000.00 15200.00 0.00 0.19057 18200.00 15200.00 0.00 0.19177 17800.00 15400.00 0.00 0.19234 18000.00 15400.00 0.00 0.19475 18200.00 15400.00 0.00 0.19586 17800.00 15600.00 0.00 0.19652 18000.00 15600.00 0.00 0.19888 18200.00 15600.00 0.00 0.19990 17800.00 15800.00 0.00 0.20063 18000.00 15800.00 0.00 0.20293 18200.00 15800.00 0.00 0.20385 17800.00 16000.00 0.00 0.20475 18000.00 16000.00 0.00 0.20698 18200.00 16000.00 0.00 0.20778 17800.00 16200.00 0.00 0.20924 18000.00 16200.00 0.00 0.21136 18200.00 16200.00 0.00 0.21201 17800.00 16400.00 0.00 0.21409 18000.00 16400.00 0.00 0.21606 18200.00 16400.00 0.00 0.21651 17800.00 16600.00 0.00 0.21899 18000.00 16600.00 0.00 0.22079 18200.00 16600.00 0.00 0.22101 17800.00 16800.00 0.00 0.22386 18000.00 16800.00 0.00 0.22544 17600.00 17000.00 0.00 0.22573 17800.00 17000.00 0.00 0.22856 18000.00 17000.00 0.00 0.22987 17600.00 17200.00 0.00 0.23021 17800.00 17200.00 0.00 0.23285 18000.00 17200.00 0.00 0.23387 17600.00 17400.00 0.00 0.23449 17800.00 17400.00 0.00 0.23691 18000.00 17400.00 0.00 0.23757 17600.00 17600.00 0.00 0.23861 17800.00 17600.00 0.00 0.24067 18000.00 17600.00 0.00 0.24083 17600.00 17800.00 0.00 0.24165 17800.00 17800.00 0.00 0.24315 17800.00 18000.00 0.00 0.24345 17800.00 18000.00 5.00 0.24356 17800.00 18000.00 10.00 0.24365 17800.00 18000.00 15.00 0.24372 17800.00 18000.00 20.00 0.24378 17800.00 18000.00 25.00 0.24381 17800.00 18000.00 30.00 0.24383 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 17765.77 17976.42 30.00 0.24389 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/009.ctf NX, NY, NZ: 360 360 1 MODE : real