CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/010.mrc Output diagnostic file name Micrographs/010.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/010.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2460.000 7542.000 Mean, RMS : 4362.790 223.5946 TITLE 1: emi2mrc: 010.emi Sun Jan 19 12:52:57 2014 TITLE 2: kV 300, Mag 78000x, Def 27890, Spot 4, Int 55.14%, Obj 88.07%, Dif 73.67% TITLE 3: X 321.268, Y 139.068, Z -112.287, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.01285 8200.00 8000.00 0.00 -0.01282 8400.00 8000.00 0.00 -0.01272 8600.00 8000.00 0.00 -0.01255 8800.00 8000.00 0.00 -0.01233 9000.00 8000.00 0.00 -0.01209 9200.00 8000.00 0.00 -0.01182 9400.00 8000.00 0.00 -0.01155 9600.00 8000.00 0.00 -0.01127 9800.00 8000.00 0.00 -0.01097 10000.00 8000.00 0.00 -0.01070 10200.00 8000.00 0.00 -0.01050 10400.00 8000.00 0.00 -0.01040 10600.00 8000.00 0.00 -0.01036 10800.00 8000.00 0.00 -0.01029 11000.00 8000.00 0.00 -0.01008 11200.00 8000.00 0.00 -0.00965 11400.00 8000.00 0.00 -0.00900 11600.00 8000.00 0.00 -0.00818 11800.00 8000.00 0.00 -0.00729 12000.00 8000.00 0.00 -0.00638 12200.00 8000.00 0.00 -0.00547 12400.00 8000.00 0.00 -0.00452 12600.00 8000.00 0.00 -0.00348 12800.00 8000.00 0.00 -0.00231 13000.00 8000.00 0.00 -0.00101 13200.00 8000.00 0.00 0.00040 13400.00 8000.00 0.00 0.00188 13600.00 8000.00 0.00 0.00341 13800.00 8000.00 0.00 0.00500 14000.00 8000.00 0.00 0.00666 14200.00 8000.00 0.00 0.00838 14400.00 8000.00 0.00 0.01016 14600.00 8000.00 0.00 0.01201 14800.00 8000.00 0.00 0.01391 15000.00 8000.00 0.00 0.01590 15200.00 8000.00 0.00 0.01798 15400.00 8000.00 0.00 0.02014 15600.00 8000.00 0.00 0.02239 15800.00 8000.00 0.00 0.02472 16000.00 8000.00 0.00 0.02715 16200.00 8000.00 0.00 0.02969 16400.00 8000.00 0.00 0.03236 16600.00 8000.00 0.00 0.03508 16800.00 8000.00 0.00 0.03775 17000.00 8000.00 0.00 0.04031 17200.00 8000.00 0.00 0.04275 17400.00 8000.00 0.00 0.04513 17600.00 8000.00 0.00 0.04760 17800.00 8000.00 0.00 0.05025 18000.00 8000.00 0.00 0.05306 18200.00 8000.00 0.00 0.05601 18400.00 8000.00 0.00 0.05904 18600.00 8000.00 0.00 0.06219 18800.00 8000.00 0.00 0.06559 19000.00 8000.00 0.00 0.06928 19200.00 8000.00 0.00 0.07316 19400.00 8000.00 0.00 0.07695 19600.00 8000.00 0.00 0.08027 19800.00 8000.00 0.00 0.08281 20000.00 8000.00 0.00 0.08449 20200.00 8000.00 0.00 0.08547 20400.00 8000.00 0.00 0.08598 20600.00 8000.00 0.00 0.08628 20800.00 8000.00 0.00 0.08645 20400.00 8200.00 0.00 0.08681 20600.00 8200.00 0.00 0.08710 20800.00 8200.00 0.00 0.08725 20400.00 8400.00 0.00 0.08762 20600.00 8400.00 0.00 0.08791 20800.00 8400.00 0.00 0.08804 20200.00 8600.00 0.00 0.08808 20400.00 8600.00 0.00 0.08859 20600.00 8600.00 0.00 0.08885 20800.00 8600.00 0.00 0.08896 20200.00 8800.00 0.00 0.08919 20400.00 8800.00 0.00 0.08968 20600.00 8800.00 0.00 0.08993 20800.00 8800.00 0.00 0.09001 20200.00 9000.00 0.00 0.09033 20400.00 9000.00 0.00 0.09082 20600.00 9000.00 0.00 0.09104 20800.00 9000.00 0.00 0.09109 20200.00 9200.00 0.00 0.09149 20400.00 9200.00 0.00 0.09196 20600.00 9200.00 0.00 0.09217 20800.00 9200.00 0.00 0.09218 20200.00 9400.00 0.00 0.09263 20400.00 9400.00 0.00 0.09310 20600.00 9400.00 0.00 0.09328 20200.00 9600.00 0.00 0.09378 20400.00 9600.00 0.00 0.09423 20600.00 9600.00 0.00 0.09438 20200.00 9800.00 0.00 0.09509 20400.00 9800.00 0.00 0.09551 20600.00 9800.00 0.00 0.09563 20000.00 10000.00 0.00 0.09565 20200.00 10000.00 0.00 0.09660 20400.00 10000.00 0.00 0.09700 20600.00 10000.00 0.00 0.09708 20000.00 10200.00 0.00 0.09731 20200.00 10200.00 0.00 0.09824 20400.00 10200.00 0.00 0.09862 20600.00 10200.00 0.00 0.09866 20000.00 10400.00 0.00 0.09903 20200.00 10400.00 0.00 0.09995 20400.00 10400.00 0.00 0.10029 20600.00 10400.00 0.00 0.10031 20000.00 10600.00 0.00 0.10078 20200.00 10600.00 0.00 0.10168 20400.00 10600.00 0.00 0.10199 20000.00 10800.00 0.00 0.10253 20200.00 10800.00 0.00 0.10340 20400.00 10800.00 0.00 0.10367 20000.00 11000.00 0.00 0.10434 20200.00 11000.00 0.00 0.10518 20400.00 11000.00 0.00 0.10541 20000.00 11200.00 0.00 0.10629 20200.00 11200.00 0.00 0.10708 20400.00 11200.00 0.00 0.10727 20000.00 11400.00 0.00 0.10826 20200.00 11400.00 0.00 0.10902 20400.00 11400.00 0.00 0.10916 20000.00 11600.00 0.00 0.11015 20200.00 11600.00 0.00 0.11088 20400.00 11600.00 0.00 0.11098 20000.00 11800.00 0.00 0.11196 20200.00 11800.00 0.00 0.11264 20400.00 11800.00 0.00 0.11270 20000.00 12000.00 0.00 0.11369 20200.00 12000.00 0.00 0.11433 20400.00 12000.00 0.00 0.11433 20000.00 12200.00 0.00 0.11547 20200.00 12200.00 0.00 0.11606 20000.00 12400.00 0.00 0.11750 20200.00 12400.00 0.00 0.11804 19800.00 12600.00 0.00 0.11835 20000.00 12600.00 0.00 0.11979 20200.00 12600.00 0.00 0.12028 19800.00 12800.00 0.00 0.12078 20000.00 12800.00 0.00 0.12218 20200.00 12800.00 0.00 0.12262 19800.00 13000.00 0.00 0.12323 20000.00 13000.00 0.00 0.12460 20200.00 13000.00 0.00 0.12500 19800.00 13200.00 0.00 0.12563 20000.00 13200.00 0.00 0.12695 20200.00 13200.00 0.00 0.12731 19800.00 13400.00 0.00 0.12795 20000.00 13400.00 0.00 0.12924 20200.00 13400.00 0.00 0.12956 19800.00 13600.00 0.00 0.13039 20000.00 13600.00 0.00 0.13165 20200.00 13600.00 0.00 0.13195 19800.00 13800.00 0.00 0.13305 20000.00 13800.00 0.00 0.13429 20200.00 13800.00 0.00 0.13459 19800.00 14000.00 0.00 0.13582 20000.00 14000.00 0.00 0.13706 20200.00 14000.00 0.00 0.13735 19800.00 14200.00 0.00 0.13865 20000.00 14200.00 0.00 0.13988 20200.00 14200.00 0.00 0.14015 19800.00 14400.00 0.00 0.14150 20000.00 14400.00 0.00 0.14273 20200.00 14400.00 0.00 0.14296 19800.00 14600.00 0.00 0.14435 20000.00 14600.00 0.00 0.14556 20200.00 14600.00 0.00 0.14576 19800.00 14800.00 0.00 0.14738 20000.00 14800.00 0.00 0.14857 20200.00 14800.00 0.00 0.14872 19800.00 15000.00 0.00 0.15076 20000.00 15000.00 0.00 0.15191 20200.00 15000.00 0.00 0.15201 19800.00 15200.00 0.00 0.15436 20000.00 15200.00 0.00 0.15546 20200.00 15200.00 0.00 0.15548 19800.00 15400.00 0.00 0.15797 20000.00 15400.00 0.00 0.15901 19600.00 15600.00 0.00 0.15904 19800.00 15600.00 0.00 0.16151 20000.00 15600.00 0.00 0.16249 19600.00 15800.00 0.00 0.16250 19800.00 15800.00 0.00 0.16493 20000.00 15800.00 0.00 0.16582 19600.00 16000.00 0.00 0.16592 19800.00 16000.00 0.00 0.16829 20000.00 16000.00 0.00 0.16909 19600.00 16200.00 0.00 0.16958 19800.00 16200.00 0.00 0.17188 20000.00 16200.00 0.00 0.17259 19600.00 16400.00 0.00 0.17351 19800.00 16400.00 0.00 0.17575 20000.00 16400.00 0.00 0.17636 19600.00 16600.00 0.00 0.17746 19800.00 16600.00 0.00 0.17963 20000.00 16600.00 0.00 0.18015 19600.00 16800.00 0.00 0.18130 19800.00 16800.00 0.00 0.18342 20000.00 16800.00 0.00 0.18383 19600.00 17000.00 0.00 0.18500 19800.00 17000.00 0.00 0.18706 20000.00 17000.00 0.00 0.18739 19600.00 17200.00 0.00 0.18861 19800.00 17200.00 0.00 0.19063 20000.00 17200.00 0.00 0.19087 19600.00 17400.00 0.00 0.19252 19800.00 17400.00 0.00 0.19448 20000.00 17400.00 0.00 0.19461 19600.00 17600.00 0.00 0.19696 19800.00 17600.00 0.00 0.19884 19600.00 17800.00 0.00 0.20167 19800.00 17800.00 0.00 0.20344 19600.00 18000.00 0.00 0.20634 19800.00 18000.00 0.00 0.20797 19600.00 18200.00 0.00 0.21081 19800.00 18200.00 0.00 0.21225 19600.00 18400.00 0.00 0.21498 19800.00 18400.00 0.00 0.21621 19600.00 18600.00 0.00 0.21926 19800.00 18600.00 0.00 0.22021 19400.00 18800.00 0.00 0.22088 19600.00 18800.00 0.00 0.22420 19800.00 18800.00 0.00 0.22474 19400.00 19000.00 0.00 0.22660 19600.00 19000.00 0.00 0.22951 19400.00 19200.00 0.00 0.23199 19600.00 19200.00 0.00 0.23429 19400.00 19400.00 0.00 0.23616 19600.00 19400.00 0.00 0.23764 19400.00 19600.00 0.00 0.23806 19600.00 19600.00 0.00 0.23852 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 19498.94 19628.13 34.44 0.23896 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/010.ctf NX, NY, NZ: 360 360 1 MODE : real