CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/011.mrc Output diagnostic file name Micrographs/011.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/011.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2132.000 9648.000 Mean, RMS : 4362.855 221.2160 TITLE 1: emi2mrc: 011.emi Sun Jan 19 12:53:57 2014 TITLE 2: kV 300, Mag 78000x, Def 27890, Spot 4, Int 55.14%, Obj 88.07%, Dif 73.67% TITLE 3: X 319.229, Y 137.394, Z -112.287, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.01160 8200.00 8000.00 0.00 -0.01134 8400.00 8000.00 0.00 -0.01082 8600.00 8000.00 0.00 -0.01010 8800.00 8000.00 0.00 -0.00931 9000.00 8000.00 0.00 -0.00852 9200.00 8000.00 0.00 -0.00780 9400.00 8000.00 0.00 -0.00715 9600.00 8000.00 0.00 -0.00651 9800.00 8000.00 0.00 -0.00588 10000.00 8000.00 0.00 -0.00524 10200.00 8000.00 0.00 -0.00460 10400.00 8000.00 0.00 -0.00396 10600.00 8000.00 0.00 -0.00328 10800.00 8000.00 0.00 -0.00253 11000.00 8000.00 0.00 -0.00169 11200.00 8000.00 0.00 -0.00080 11400.00 8000.00 0.00 0.00012 11600.00 8000.00 0.00 0.00109 11800.00 8000.00 0.00 0.00216 12000.00 8000.00 0.00 0.00342 12200.00 8000.00 0.00 0.00491 12400.00 8000.00 0.00 0.00659 12600.00 8000.00 0.00 0.00834 12800.00 8000.00 0.00 0.01005 13000.00 8000.00 0.00 0.01164 13200.00 8000.00 0.00 0.01316 13400.00 8000.00 0.00 0.01474 13600.00 8000.00 0.00 0.01652 13800.00 8000.00 0.00 0.01857 14000.00 8000.00 0.00 0.02088 14200.00 8000.00 0.00 0.02337 14400.00 8000.00 0.00 0.02598 14600.00 8000.00 0.00 0.02867 14800.00 8000.00 0.00 0.03140 15000.00 8000.00 0.00 0.03416 15200.00 8000.00 0.00 0.03694 15400.00 8000.00 0.00 0.03969 15600.00 8000.00 0.00 0.04245 15800.00 8000.00 0.00 0.04530 16000.00 8000.00 0.00 0.04833 16200.00 8000.00 0.00 0.05161 16400.00 8000.00 0.00 0.05511 16600.00 8000.00 0.00 0.05874 16800.00 8000.00 0.00 0.06244 17000.00 8000.00 0.00 0.06628 17200.00 8000.00 0.00 0.07037 17400.00 8000.00 0.00 0.07480 17600.00 8000.00 0.00 0.07952 17800.00 8000.00 0.00 0.08421 18000.00 8000.00 0.00 0.08838 18200.00 8000.00 0.00 0.09158 18400.00 8000.00 0.00 0.09360 18600.00 8000.00 0.00 0.09458 18800.00 8000.00 0.00 0.09491 19000.00 8000.00 0.00 0.09499 18600.00 8200.00 0.00 0.09557 18800.00 8200.00 0.00 0.09589 19000.00 8200.00 0.00 0.09595 18600.00 8400.00 0.00 0.09661 18800.00 8400.00 0.00 0.09692 19000.00 8400.00 0.00 0.09696 18400.00 8600.00 0.00 0.09697 18600.00 8600.00 0.00 0.09790 18800.00 8600.00 0.00 0.09819 19000.00 8600.00 0.00 0.09823 18400.00 8800.00 0.00 0.09850 18600.00 8800.00 0.00 0.09942 18800.00 8800.00 0.00 0.09971 19000.00 8800.00 0.00 0.09974 18400.00 9000.00 0.00 0.10015 18600.00 9000.00 0.00 0.10107 18800.00 9000.00 0.00 0.10135 19000.00 9000.00 0.00 0.10139 18400.00 9200.00 0.00 0.10189 18600.00 9200.00 0.00 0.10280 18800.00 9200.00 0.00 0.10309 19000.00 9200.00 0.00 0.10313 18400.00 9400.00 0.00 0.10365 18600.00 9400.00 0.00 0.10457 18800.00 9400.00 0.00 0.10487 19000.00 9400.00 0.00 0.10492 18400.00 9600.00 0.00 0.10543 18600.00 9600.00 0.00 0.10637 18800.00 9600.00 0.00 0.10667 19000.00 9600.00 0.00 0.10673 18400.00 9800.00 0.00 0.10735 18600.00 9800.00 0.00 0.10830 18800.00 9800.00 0.00 0.10860 19000.00 9800.00 0.00 0.10865 18400.00 10000.00 0.00 0.10939 18600.00 10000.00 0.00 0.11034 18800.00 10000.00 0.00 0.11064 19000.00 10000.00 0.00 0.11067 18400.00 10200.00 0.00 0.11137 18600.00 10200.00 0.00 0.11233 18800.00 10200.00 0.00 0.11262 19000.00 10200.00 0.00 0.11264 18400.00 10400.00 0.00 0.11326 18600.00 10400.00 0.00 0.11422 18800.00 10400.00 0.00 0.11451 18400.00 10600.00 0.00 0.11509 18600.00 10600.00 0.00 0.11607 18800.00 10600.00 0.00 0.11636 18400.00 10800.00 0.00 0.11695 18600.00 10800.00 0.00 0.11794 18800.00 10800.00 0.00 0.11823 18400.00 11000.00 0.00 0.11905 18600.00 11000.00 0.00 0.12005 18800.00 11000.00 0.00 0.12032 18400.00 11200.00 0.00 0.12152 18600.00 11200.00 0.00 0.12251 18800.00 11200.00 0.00 0.12275 18400.00 11400.00 0.00 0.12416 18600.00 11400.00 0.00 0.12513 18800.00 11400.00 0.00 0.12531 18400.00 11600.00 0.00 0.12676 18600.00 11600.00 0.00 0.12769 18800.00 11600.00 0.00 0.12780 18400.00 11800.00 0.00 0.12922 18600.00 11800.00 0.00 0.13009 18800.00 11800.00 0.00 0.13011 18400.00 12000.00 0.00 0.13148 18600.00 12000.00 0.00 0.13228 18400.00 12200.00 0.00 0.13372 18600.00 12200.00 0.00 0.13445 18400.00 12400.00 0.00 0.13623 18600.00 12400.00 0.00 0.13689 18200.00 12600.00 0.00 0.13707 18400.00 12600.00 0.00 0.13907 18600.00 12600.00 0.00 0.13967 18200.00 12800.00 0.00 0.14011 18400.00 12800.00 0.00 0.14208 18600.00 12800.00 0.00 0.14262 18200.00 13000.00 0.00 0.14319 18400.00 13000.00 0.00 0.14513 18600.00 13000.00 0.00 0.14563 18200.00 13200.00 0.00 0.14620 18400.00 13200.00 0.00 0.14811 18600.00 13200.00 0.00 0.14854 18200.00 13400.00 0.00 0.14909 18400.00 13400.00 0.00 0.15096 18600.00 13400.00 0.00 0.15133 18200.00 13600.00 0.00 0.15211 18400.00 13600.00 0.00 0.15394 18600.00 13600.00 0.00 0.15425 18200.00 13800.00 0.00 0.15542 18400.00 13800.00 0.00 0.15721 18600.00 13800.00 0.00 0.15746 18200.00 14000.00 0.00 0.15889 18400.00 14000.00 0.00 0.16064 18600.00 14000.00 0.00 0.16083 18200.00 14200.00 0.00 0.16244 18400.00 14200.00 0.00 0.16416 18600.00 14200.00 0.00 0.16428 18200.00 14400.00 0.00 0.16609 18400.00 14400.00 0.00 0.16778 18600.00 14400.00 0.00 0.16781 18200.00 14600.00 0.00 0.16979 18400.00 14600.00 0.00 0.17143 18200.00 14800.00 0.00 0.17370 18400.00 14800.00 0.00 0.17528 18200.00 15000.00 0.00 0.17804 18400.00 15000.00 0.00 0.17956 18200.00 15200.00 0.00 0.18267 18400.00 15200.00 0.00 0.18410 18200.00 15400.00 0.00 0.18731 18400.00 15400.00 0.00 0.18866 18200.00 15600.00 0.00 0.19190 18400.00 15600.00 0.00 0.19314 18200.00 15800.00 0.00 0.19637 18400.00 15800.00 0.00 0.19747 18000.00 16000.00 0.00 0.19760 18200.00 16000.00 0.00 0.20085 18400.00 16000.00 0.00 0.20179 18000.00 16200.00 0.00 0.20260 18200.00 16200.00 0.00 0.20574 18400.00 16200.00 0.00 0.20646 18000.00 16400.00 0.00 0.20806 18200.00 16400.00 0.00 0.21104 18400.00 16400.00 0.00 0.21150 18000.00 16600.00 0.00 0.21363 18200.00 16600.00 0.00 0.21640 18400.00 16600.00 0.00 0.21655 18000.00 16800.00 0.00 0.21911 18200.00 16800.00 0.00 0.22164 18000.00 17000.00 0.00 0.22435 18200.00 17000.00 0.00 0.22657 18000.00 17200.00 0.00 0.22928 18200.00 17200.00 0.00 0.23115 18000.00 17400.00 0.00 0.23430 18200.00 17400.00 0.00 0.23574 18000.00 17600.00 0.00 0.23956 18200.00 17600.00 0.00 0.24041 17800.00 17800.00 0.00 0.24094 18000.00 17800.00 0.00 0.24415 18200.00 17800.00 0.00 0.24425 17800.00 18000.00 0.00 0.24461 18000.00 18000.00 0.00 0.24704 18000.00 18200.00 0.00 0.24760 18000.00 18200.00 5.00 0.24770 18000.00 18200.00 10.00 0.24779 18000.00 18200.00 15.00 0.24784 18000.00 18200.00 20.00 0.24788 18000.00 18200.00 25.00 0.24788 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 17990.91 18174.09 23.53 0.24792 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/011.ctf NX, NY, NZ: 360 360 1 MODE : real