CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/022.mrc Output diagnostic file name Micrographs/022.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/022.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2326.000 8030.000 Mean, RMS : 4399.845 224.7693 TITLE 1: emi2mrc: 022.emi Sun Jan 19 14:06:15 2014 TITLE 2: kV 300, Mag 78000x, Def 23300, Spot 4, Int 55.14%, Obj 88.04%, Dif 73.67% TITLE 3: X 235.721, Y 172.321, Z -28.282, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.04244 21600.00 8000.00 0.00 -0.04221 21800.00 8000.00 0.00 -0.04107 22000.00 8000.00 0.00 -0.03998 22200.00 8000.00 0.00 -0.03897 22400.00 8000.00 0.00 -0.03803 22600.00 8000.00 0.00 -0.03716 22800.00 8000.00 0.00 -0.03631 23000.00 8000.00 0.00 -0.03543 23200.00 8000.00 0.00 -0.03450 23400.00 8000.00 0.00 -0.03350 23600.00 8000.00 0.00 -0.03242 23800.00 8000.00 0.00 -0.03124 24000.00 8000.00 0.00 -0.02996 24200.00 8000.00 0.00 -0.02855 24400.00 8000.00 0.00 -0.02702 24600.00 8000.00 0.00 -0.02539 24800.00 8000.00 0.00 -0.02369 25000.00 8000.00 0.00 -0.02195 25200.00 8000.00 0.00 -0.02018 25400.00 8000.00 0.00 -0.01838 25600.00 8000.00 0.00 -0.01654 25800.00 8000.00 0.00 -0.01468 26000.00 8000.00 0.00 -0.01282 26200.00 8000.00 0.00 -0.01100 26400.00 8000.00 0.00 -0.00923 26600.00 8000.00 0.00 -0.00749 26800.00 8000.00 0.00 -0.00573 27000.00 8000.00 0.00 -0.00387 27200.00 8000.00 0.00 -0.00184 27400.00 8000.00 0.00 0.00039 27600.00 8000.00 0.00 0.00286 27800.00 8000.00 0.00 0.00555 28000.00 8000.00 0.00 0.00836 28200.00 8000.00 0.00 0.01112 28400.00 8000.00 0.00 0.01362 28600.00 8000.00 0.00 0.01559 28800.00 8000.00 0.00 0.01686 29000.00 8000.00 0.00 0.01742 29000.00 8200.00 0.00 0.01744 29200.00 9000.00 0.00 0.01747 29000.00 9200.00 0.00 0.01759 29200.00 9200.00 0.00 0.01766 29000.00 9400.00 0.00 0.01786 29200.00 9400.00 0.00 0.01795 29000.00 9600.00 0.00 0.01824 29200.00 9600.00 0.00 0.01834 29000.00 9800.00 0.00 0.01868 29200.00 9800.00 0.00 0.01878 29000.00 10000.00 0.00 0.01913 29200.00 10000.00 0.00 0.01921 29000.00 10200.00 0.00 0.01949 29200.00 10200.00 0.00 0.01953 29000.00 10400.00 0.00 0.01973 29000.00 10600.00 0.00 0.01984 29000.00 10800.00 0.00 0.01991 29000.00 11000.00 0.00 0.02002 29000.00 11200.00 0.00 0.02027 29000.00 11400.00 0.00 0.02066 29000.00 11600.00 0.00 0.02119 28800.00 11800.00 0.00 0.02122 29000.00 11800.00 0.00 0.02183 28800.00 12000.00 0.00 0.02190 29000.00 12000.00 0.00 0.02250 28800.00 12200.00 0.00 0.02261 29000.00 12200.00 0.00 0.02319 28800.00 12400.00 0.00 0.02331 29000.00 12400.00 0.00 0.02386 28800.00 12600.00 0.00 0.02397 29000.00 12600.00 0.00 0.02448 28800.00 12800.00 0.00 0.02453 29000.00 12800.00 0.00 0.02500 29000.00 13000.00 0.00 0.02542 29000.00 13200.00 0.00 0.02576 29000.00 13400.00 0.00 0.02609 28800.00 13600.00 0.00 0.02613 29000.00 13600.00 0.00 0.02648 28800.00 13800.00 0.00 0.02661 29000.00 13800.00 0.00 0.02696 28800.00 14000.00 0.00 0.02714 29000.00 14000.00 0.00 0.02750 28800.00 14200.00 0.00 0.02767 29000.00 14200.00 0.00 0.02804 28800.00 14400.00 0.00 0.02815 29000.00 14400.00 0.00 0.02854 28800.00 14600.00 0.00 0.02860 29000.00 14600.00 0.00 0.02900 28800.00 14800.00 0.00 0.02909 29000.00 14800.00 0.00 0.02949 28800.00 15000.00 0.00 0.02970 29000.00 15000.00 0.00 0.03011 28800.00 15200.00 0.00 0.03049 29000.00 15200.00 0.00 0.03089 28800.00 15400.00 0.00 0.03144 29000.00 15400.00 0.00 0.03183 28800.00 15600.00 0.00 0.03251 29000.00 15600.00 0.00 0.03288 28800.00 15800.00 0.00 0.03362 29000.00 15800.00 0.00 0.03398 28800.00 16000.00 0.00 0.03473 29000.00 16000.00 0.00 0.03509 28800.00 16200.00 0.00 0.03588 29000.00 16200.00 0.00 0.03623 28800.00 16400.00 0.00 0.03708 29000.00 16400.00 0.00 0.03744 28800.00 16600.00 0.00 0.03837 29000.00 16600.00 0.00 0.03874 28800.00 16800.00 0.00 0.03976 29000.00 16800.00 0.00 0.04014 28800.00 17000.00 0.00 0.04122 29000.00 17000.00 0.00 0.04160 28800.00 17200.00 0.00 0.04270 29000.00 17200.00 0.00 0.04308 28800.00 17400.00 0.00 0.04419 29000.00 17400.00 0.00 0.04457 28800.00 17600.00 0.00 0.04567 29000.00 17600.00 0.00 0.04605 28800.00 17800.00 0.00 0.04709 29000.00 17800.00 0.00 0.04747 28800.00 18000.00 0.00 0.04846 29000.00 18000.00 0.00 0.04884 28800.00 18200.00 0.00 0.04981 29000.00 18200.00 0.00 0.05019 28800.00 18400.00 0.00 0.05116 29000.00 18400.00 0.00 0.05155 28800.00 18600.00 0.00 0.05257 29000.00 18600.00 0.00 0.05296 28800.00 18800.00 0.00 0.05409 29000.00 18800.00 0.00 0.05447 28800.00 19000.00 0.00 0.05569 29000.00 19000.00 0.00 0.05603 28800.00 19200.00 0.00 0.05730 29000.00 19200.00 0.00 0.05761 28600.00 19400.00 0.00 0.05764 28800.00 19400.00 0.00 0.05891 29000.00 19400.00 0.00 0.05920 28600.00 19600.00 0.00 0.05928 28800.00 19600.00 0.00 0.06053 29000.00 19600.00 0.00 0.06077 28600.00 19800.00 0.00 0.06096 28800.00 19800.00 0.00 0.06217 29000.00 19800.00 0.00 0.06238 28600.00 20000.00 0.00 0.06276 28800.00 20000.00 0.00 0.06393 29000.00 20000.00 0.00 0.06408 28600.00 20200.00 0.00 0.06467 28800.00 20200.00 0.00 0.06579 29000.00 20200.00 0.00 0.06588 28600.00 20400.00 0.00 0.06661 28800.00 20400.00 0.00 0.06768 29000.00 20400.00 0.00 0.06773 28600.00 20600.00 0.00 0.06852 28800.00 20600.00 0.00 0.06954 29000.00 20600.00 0.00 0.06956 28600.00 20800.00 0.00 0.07032 28800.00 20800.00 0.00 0.07132 28600.00 21000.00 0.00 0.07201 28800.00 21000.00 0.00 0.07298 28600.00 21200.00 0.00 0.07367 28800.00 21200.00 0.00 0.07463 28600.00 21400.00 0.00 0.07541 28800.00 21400.00 0.00 0.07637 28600.00 21600.00 0.00 0.07724 28800.00 21600.00 0.00 0.07821 28600.00 21800.00 0.00 0.07920 28800.00 21800.00 0.00 0.08018 28600.00 22000.00 0.00 0.08127 28800.00 22000.00 0.00 0.08225 28600.00 22200.00 0.00 0.08337 28800.00 22200.00 0.00 0.08434 28600.00 22400.00 0.00 0.08547 28800.00 22400.00 0.00 0.08642 28600.00 22600.00 0.00 0.08763 28800.00 22600.00 0.00 0.08856 28600.00 22800.00 0.00 0.08981 28800.00 22800.00 0.00 0.09072 28600.00 23000.00 0.00 0.09198 28800.00 23000.00 0.00 0.09288 28600.00 23200.00 0.00 0.09418 28800.00 23200.00 0.00 0.09506 28600.00 23400.00 0.00 0.09638 28800.00 23400.00 0.00 0.09725 28600.00 23600.00 0.00 0.09859 28800.00 23600.00 0.00 0.09945 28600.00 23800.00 0.00 0.10093 28800.00 23800.00 0.00 0.10178 28600.00 24000.00 0.00 0.10343 28800.00 24000.00 0.00 0.10426 28600.00 24200.00 0.00 0.10601 28800.00 24200.00 0.00 0.10680 28600.00 24400.00 0.00 0.10866 28800.00 24400.00 0.00 0.10941 28600.00 24600.00 0.00 0.11140 28800.00 24600.00 0.00 0.11208 28600.00 24800.00 0.00 0.11413 28800.00 24800.00 0.00 0.11474 28400.00 25000.00 0.00 0.11476 28600.00 25000.00 0.00 0.11695 28800.00 25000.00 0.00 0.11747 28400.00 25200.00 0.00 0.11780 28600.00 25200.00 0.00 0.11993 28800.00 25200.00 0.00 0.12036 28400.00 25400.00 0.00 0.12090 28600.00 25400.00 0.00 0.12298 28800.00 25400.00 0.00 0.12331 28400.00 25600.00 0.00 0.12403 28600.00 25600.00 0.00 0.12603 28800.00 25600.00 0.00 0.12624 28400.00 25800.00 0.00 0.12722 28600.00 25800.00 0.00 0.12915 28800.00 25800.00 0.00 0.12923 28400.00 26000.00 0.00 0.13045 28600.00 26000.00 0.00 0.13229 28400.00 26200.00 0.00 0.13377 28600.00 26200.00 0.00 0.13552 28400.00 26400.00 0.00 0.13736 28600.00 26400.00 0.00 0.13898 28400.00 26600.00 0.00 0.14109 28600.00 26600.00 0.00 0.14257 28400.00 26800.00 0.00 0.14472 28600.00 26800.00 0.00 0.14603 28400.00 27000.00 0.00 0.14821 28600.00 27000.00 0.00 0.14932 28400.00 27200.00 0.00 0.15150 28600.00 27200.00 0.00 0.15237 28400.00 27400.00 0.00 0.15459 28600.00 27400.00 0.00 0.15520 28400.00 27600.00 0.00 0.15780 28600.00 27600.00 0.00 0.15809 28200.00 27800.00 0.00 0.15873 28400.00 27800.00 0.00 0.16113 28200.00 28000.00 0.00 0.16202 28400.00 28000.00 0.00 0.16401 28200.00 28200.00 0.00 0.16447 28400.00 28200.00 0.00 0.16594 28400.00 28400.00 0.00 0.16653 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 28310.37 28444.89 40.99 0.16674 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/022.ctf NX, NY, NZ: 360 360 1 MODE : real