CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/031.mrc Output diagnostic file name Micrographs/031.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/031.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2024.000 8772.000 Mean, RMS : 4362.104 220.9343 TITLE 1: emi2mrc: 031.emi Sun Jan 19 12:51:59 2014 TITLE 2: kV 300, Mag 78000x, Def 27890, Spot 4, Int 55.14%, Obj 88.07%, Dif 73.67% TITLE 3: X 323.411, Y 140.576, Z -112.287, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 119 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.01644 11000.00 8000.00 0.00 -0.01644 11200.00 8000.00 0.00 -0.01622 11400.00 8000.00 0.00 -0.01600 11600.00 8000.00 0.00 -0.01574 11800.00 8000.00 0.00 -0.01539 12000.00 8000.00 0.00 -0.01489 12200.00 8000.00 0.00 -0.01425 12400.00 8000.00 0.00 -0.01352 12600.00 8000.00 0.00 -0.01281 12800.00 8000.00 0.00 -0.01219 13000.00 8000.00 0.00 -0.01162 13200.00 8000.00 0.00 -0.01100 13400.00 8000.00 0.00 -0.01019 13600.00 8000.00 0.00 -0.00907 13800.00 8000.00 0.00 -0.00766 14000.00 8000.00 0.00 -0.00603 14200.00 8000.00 0.00 -0.00429 14400.00 8000.00 0.00 -0.00254 14600.00 8000.00 0.00 -0.00080 14800.00 8000.00 0.00 0.00094 15000.00 8000.00 0.00 0.00271 15200.00 8000.00 0.00 0.00452 15400.00 8000.00 0.00 0.00637 15600.00 8000.00 0.00 0.00822 15800.00 8000.00 0.00 0.01003 16000.00 8000.00 0.00 0.01177 16200.00 8000.00 0.00 0.01345 16400.00 8000.00 0.00 0.01511 16600.00 8000.00 0.00 0.01679 16800.00 8000.00 0.00 0.01856 17000.00 8000.00 0.00 0.02049 17200.00 8000.00 0.00 0.02259 17400.00 8000.00 0.00 0.02485 17600.00 8000.00 0.00 0.02721 17800.00 8000.00 0.00 0.02961 18000.00 8000.00 0.00 0.03203 18200.00 8000.00 0.00 0.03447 18400.00 8000.00 0.00 0.03701 18600.00 8000.00 0.00 0.03970 18800.00 8000.00 0.00 0.04257 19000.00 8000.00 0.00 0.04561 19200.00 8000.00 0.00 0.04875 19400.00 8000.00 0.00 0.05201 19600.00 8000.00 0.00 0.05542 19800.00 8000.00 0.00 0.05905 20000.00 8000.00 0.00 0.06288 20200.00 8000.00 0.00 0.06674 20400.00 8000.00 0.00 0.07026 20600.00 8000.00 0.00 0.07309 20800.00 8000.00 0.00 0.07495 21000.00 8000.00 0.00 0.07581 21200.00 8000.00 0.00 0.07592 21000.00 8200.00 0.00 0.07662 21200.00 8200.00 0.00 0.07674 21000.00 8400.00 0.00 0.07736 21200.00 8400.00 0.00 0.07749 21000.00 8600.00 0.00 0.07817 21200.00 8600.00 0.00 0.07830 21000.00 8800.00 0.00 0.07908 21200.00 8800.00 0.00 0.07920 21000.00 9000.00 0.00 0.08002 21200.00 9000.00 0.00 0.08014 21000.00 9200.00 0.00 0.08101 21200.00 9200.00 0.00 0.08112 21000.00 9400.00 0.00 0.08200 21200.00 9400.00 0.00 0.08209 20800.00 9600.00 0.00 0.08210 21000.00 9600.00 0.00 0.08299 21200.00 9600.00 0.00 0.08307 20800.00 9800.00 0.00 0.08322 21000.00 9800.00 0.00 0.08410 21200.00 9800.00 0.00 0.08416 20800.00 10000.00 0.00 0.08452 21000.00 10000.00 0.00 0.08538 21200.00 10000.00 0.00 0.08542 20800.00 10200.00 0.00 0.08590 21000.00 10200.00 0.00 0.08674 21200.00 10200.00 0.00 0.08674 20800.00 10400.00 0.00 0.08731 21000.00 10400.00 0.00 0.08812 20800.00 10600.00 0.00 0.08871 21000.00 10600.00 0.00 0.08949 20800.00 10800.00 0.00 0.09007 21000.00 10800.00 0.00 0.09083 20800.00 11000.00 0.00 0.09152 21000.00 11000.00 0.00 0.09227 20800.00 11200.00 0.00 0.09316 21000.00 11200.00 0.00 0.09394 20800.00 11400.00 0.00 0.09499 21000.00 11400.00 0.00 0.09579 20800.00 11600.00 0.00 0.09695 21000.00 11600.00 0.00 0.09777 20800.00 11800.00 0.00 0.09898 21000.00 11800.00 0.00 0.09983 20800.00 12000.00 0.00 0.10102 21000.00 12000.00 0.00 0.10190 20800.00 12200.00 0.00 0.10307 21000.00 12200.00 0.00 0.10396 20800.00 12400.00 0.00 0.10521 21000.00 12400.00 0.00 0.10609 20800.00 12600.00 0.00 0.10736 21000.00 12600.00 0.00 0.10821 20800.00 12800.00 0.00 0.10942 21000.00 12800.00 0.00 0.11023 20800.00 13000.00 0.00 0.11140 21000.00 13000.00 0.00 0.11215 20800.00 13200.00 0.00 0.11330 21000.00 13200.00 0.00 0.11401 20800.00 13400.00 0.00 0.11523 21000.00 13400.00 0.00 0.11590 20800.00 13600.00 0.00 0.11738 21000.00 13600.00 0.00 0.11803 20800.00 13800.00 0.00 0.11985 21000.00 13800.00 0.00 0.12050 20600.00 14000.00 0.00 0.12056 20800.00 14000.00 0.00 0.12252 21000.00 14000.00 0.00 0.12317 20600.00 14200.00 0.00 0.12332 20800.00 14200.00 0.00 0.12530 21000.00 14200.00 0.00 0.12596 20600.00 14400.00 0.00 0.12615 20800.00 14400.00 0.00 0.12815 21000.00 14400.00 0.00 0.12882 20600.00 14600.00 0.00 0.12896 20800.00 14600.00 0.00 0.13098 21000.00 14600.00 0.00 0.13163 20600.00 14800.00 0.00 0.13183 20800.00 14800.00 0.00 0.13385 21000.00 14800.00 0.00 0.13447 20600.00 15000.00 0.00 0.13487 20800.00 15000.00 0.00 0.13686 21000.00 15000.00 0.00 0.13743 20600.00 15200.00 0.00 0.13793 20800.00 15200.00 0.00 0.13989 21000.00 15200.00 0.00 0.14040 20600.00 15400.00 0.00 0.14090 20800.00 15400.00 0.00 0.14284 21000.00 15400.00 0.00 0.14329 20600.00 15600.00 0.00 0.14380 20800.00 15600.00 0.00 0.14570 21000.00 15600.00 0.00 0.14609 20600.00 15800.00 0.00 0.14661 20800.00 15800.00 0.00 0.14848 21000.00 15800.00 0.00 0.14881 20600.00 16000.00 0.00 0.14946 20800.00 16000.00 0.00 0.15130 21000.00 16000.00 0.00 0.15155 20600.00 16200.00 0.00 0.15261 20800.00 16200.00 0.00 0.15441 21000.00 16200.00 0.00 0.15459 20600.00 16400.00 0.00 0.15609 20800.00 16400.00 0.00 0.15783 21000.00 16400.00 0.00 0.15793 20600.00 16600.00 0.00 0.15966 20800.00 16600.00 0.00 0.16134 21000.00 16600.00 0.00 0.16136 20600.00 16800.00 0.00 0.16322 20800.00 16800.00 0.00 0.16483 20600.00 17000.00 0.00 0.16669 20800.00 17000.00 0.00 0.16822 20600.00 17200.00 0.00 0.17001 20800.00 17200.00 0.00 0.17146 20600.00 17400.00 0.00 0.17341 20800.00 17400.00 0.00 0.17478 20600.00 17600.00 0.00 0.17709 20800.00 17600.00 0.00 0.17839 20600.00 17800.00 0.00 0.18093 20800.00 17800.00 0.00 0.18216 20600.00 18000.00 0.00 0.18485 20800.00 18000.00 0.00 0.18600 20600.00 18200.00 0.00 0.18882 20800.00 18200.00 0.00 0.18988 20600.00 18400.00 0.00 0.19275 20800.00 18400.00 0.00 0.19370 20400.00 18600.00 0.00 0.19387 20600.00 18600.00 0.00 0.19674 20800.00 18600.00 0.00 0.19756 20400.00 18800.00 0.00 0.19825 20600.00 18800.00 0.00 0.20102 20800.00 18800.00 0.00 0.20167 20400.00 19000.00 0.00 0.20279 20600.00 19000.00 0.00 0.20543 20800.00 19000.00 0.00 0.20587 20400.00 19200.00 0.00 0.20725 20600.00 19200.00 0.00 0.20973 20800.00 19200.00 0.00 0.20994 20400.00 19400.00 0.00 0.21163 20600.00 19400.00 0.00 0.21392 20400.00 19600.00 0.00 0.21582 20600.00 19600.00 0.00 0.21787 20400.00 19800.00 0.00 0.21982 20600.00 19800.00 0.00 0.22159 20400.00 20000.00 0.00 0.22394 20600.00 20000.00 0.00 0.22530 20400.00 20200.00 0.00 0.22791 20600.00 20200.00 0.00 0.22870 20400.00 20400.00 0.00 0.23072 20600.00 20400.00 0.00 0.23078 20400.00 20600.00 0.00 0.23165 20400.00 20600.00 5.00 0.23174 20400.00 20600.00 10.00 0.23181 20400.00 20600.00 15.00 0.23187 20400.00 20600.00 20.00 0.23191 20400.00 20600.00 25.00 0.23193 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 20421.62 20600.00 25.00 0.23194 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/031.ctf NX, NY, NZ: 360 360 1 MODE : real