CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/049.mrc Output diagnostic file name Micrographs/049.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/049.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2472.000 9782.000 Mean, RMS : 4422.633 223.8253 TITLE 1: emi2mrc: 049.emi Sun Jan 19 14:43:30 2014 TITLE 2: kV 300, Mag 78000x, Def 26580, Spot 4, Int 55.14%, Obj 88.03%, Dif 73.67% TITLE 3: X 279.992, Y -466.338, Z -57.737, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.01884 8200.00 8000.00 0.00 -0.01866 8400.00 8000.00 0.00 -0.01840 8600.00 8000.00 0.00 -0.01814 8800.00 8000.00 0.00 -0.01792 9000.00 8000.00 0.00 -0.01771 9200.00 8000.00 0.00 -0.01749 9400.00 8000.00 0.00 -0.01729 9600.00 8000.00 0.00 -0.01713 9800.00 8000.00 0.00 -0.01700 10000.00 8000.00 0.00 -0.01693 10200.00 8000.00 0.00 -0.01683 10400.00 8000.00 0.00 -0.01660 10600.00 8000.00 0.00 -0.01620 10800.00 8000.00 0.00 -0.01564 11000.00 8000.00 0.00 -0.01497 11200.00 8000.00 0.00 -0.01429 11400.00 8000.00 0.00 -0.01363 11600.00 8000.00 0.00 -0.01296 11800.00 8000.00 0.00 -0.01222 12000.00 8000.00 0.00 -0.01137 12200.00 8000.00 0.00 -0.01036 12400.00 8000.00 0.00 -0.00922 12600.00 8000.00 0.00 -0.00802 12800.00 8000.00 0.00 -0.00680 13000.00 8000.00 0.00 -0.00559 13200.00 8000.00 0.00 -0.00440 13400.00 8000.00 0.00 -0.00322 13600.00 8000.00 0.00 -0.00199 13800.00 8000.00 0.00 -0.00068 14000.00 8000.00 0.00 0.00074 14200.00 8000.00 0.00 0.00226 14400.00 8000.00 0.00 0.00386 14600.00 8000.00 0.00 0.00550 14800.00 8000.00 0.00 0.00718 15000.00 8000.00 0.00 0.00892 15200.00 8000.00 0.00 0.01078 15400.00 8000.00 0.00 0.01279 15600.00 8000.00 0.00 0.01492 15800.00 8000.00 0.00 0.01713 16000.00 8000.00 0.00 0.01940 16200.00 8000.00 0.00 0.02172 16400.00 8000.00 0.00 0.02414 16600.00 8000.00 0.00 0.02668 16800.00 8000.00 0.00 0.02933 17000.00 8000.00 0.00 0.03206 17200.00 8000.00 0.00 0.03477 17400.00 8000.00 0.00 0.03746 17600.00 8000.00 0.00 0.04018 17800.00 8000.00 0.00 0.04298 18000.00 8000.00 0.00 0.04592 18200.00 8000.00 0.00 0.04902 18400.00 8000.00 0.00 0.05227 18600.00 8000.00 0.00 0.05567 18800.00 8000.00 0.00 0.05933 19000.00 8000.00 0.00 0.06332 19200.00 8000.00 0.00 0.06760 19400.00 8000.00 0.00 0.07198 19600.00 8000.00 0.00 0.07611 19800.00 8000.00 0.00 0.07956 20000.00 8000.00 0.00 0.08210 20200.00 8000.00 0.00 0.08369 20400.00 8000.00 0.00 0.08447 20600.00 8000.00 0.00 0.08474 20400.00 8200.00 0.00 0.08543 20600.00 8200.00 0.00 0.08567 20400.00 8400.00 0.00 0.08636 20600.00 8400.00 0.00 0.08659 20200.00 8600.00 0.00 0.08667 20400.00 8600.00 0.00 0.08743 20600.00 8600.00 0.00 0.08765 20200.00 8800.00 0.00 0.08788 20400.00 8800.00 0.00 0.08865 20600.00 8800.00 0.00 0.08887 20200.00 9000.00 0.00 0.08915 20400.00 9000.00 0.00 0.08993 20600.00 9000.00 0.00 0.09017 20200.00 9200.00 0.00 0.09045 20400.00 9200.00 0.00 0.09126 20600.00 9200.00 0.00 0.09150 20200.00 9400.00 0.00 0.09173 20400.00 9400.00 0.00 0.09256 20600.00 9400.00 0.00 0.09280 20200.00 9600.00 0.00 0.09299 20400.00 9600.00 0.00 0.09383 20600.00 9600.00 0.00 0.09405 20200.00 9800.00 0.00 0.09432 20400.00 9800.00 0.00 0.09515 20600.00 9800.00 0.00 0.09534 20200.00 10000.00 0.00 0.09575 20400.00 10000.00 0.00 0.09657 20600.00 10000.00 0.00 0.09672 20200.00 10200.00 0.00 0.09720 20400.00 10200.00 0.00 0.09799 20600.00 10200.00 0.00 0.09811 20200.00 10400.00 0.00 0.09863 20400.00 10400.00 0.00 0.09941 20600.00 10400.00 0.00 0.09948 20200.00 10600.00 0.00 0.10008 20400.00 10600.00 0.00 0.10083 20600.00 10600.00 0.00 0.10085 20200.00 10800.00 0.00 0.10155 20400.00 10800.00 0.00 0.10225 20200.00 11000.00 0.00 0.10311 20400.00 11000.00 0.00 0.10376 20200.00 11200.00 0.00 0.10482 20400.00 11200.00 0.00 0.10541 20200.00 11400.00 0.00 0.10655 20400.00 11400.00 0.00 0.10708 20200.00 11600.00 0.00 0.10821 20400.00 11600.00 0.00 0.10870 20200.00 11800.00 0.00 0.10983 20400.00 11800.00 0.00 0.11029 20200.00 12000.00 0.00 0.11146 20400.00 12000.00 0.00 0.11190 20200.00 12200.00 0.00 0.11327 20400.00 12200.00 0.00 0.11369 20000.00 12400.00 0.00 0.11392 20200.00 12400.00 0.00 0.11542 20400.00 12400.00 0.00 0.11582 20000.00 12600.00 0.00 0.11636 20200.00 12600.00 0.00 0.11784 20400.00 12600.00 0.00 0.11821 20000.00 12800.00 0.00 0.11884 20200.00 12800.00 0.00 0.12030 20400.00 12800.00 0.00 0.12063 20000.00 13000.00 0.00 0.12122 20200.00 13000.00 0.00 0.12264 20400.00 13000.00 0.00 0.12292 20000.00 13200.00 0.00 0.12343 20200.00 13200.00 0.00 0.12480 20400.00 13200.00 0.00 0.12501 20000.00 13400.00 0.00 0.12550 20200.00 13400.00 0.00 0.12683 20400.00 13400.00 0.00 0.12699 20000.00 13600.00 0.00 0.12772 20200.00 13600.00 0.00 0.12902 20400.00 13600.00 0.00 0.12914 20000.00 13800.00 0.00 0.13025 20200.00 13800.00 0.00 0.13152 20400.00 13800.00 0.00 0.13161 20000.00 14000.00 0.00 0.13298 20200.00 14000.00 0.00 0.13423 20400.00 14000.00 0.00 0.13428 20000.00 14200.00 0.00 0.13578 20200.00 14200.00 0.00 0.13700 20000.00 14400.00 0.00 0.13858 20200.00 14400.00 0.00 0.13977 20000.00 14600.00 0.00 0.14132 20200.00 14600.00 0.00 0.14246 20000.00 14800.00 0.00 0.14417 20200.00 14800.00 0.00 0.14527 20000.00 15000.00 0.00 0.14736 20200.00 15000.00 0.00 0.14840 20000.00 15200.00 0.00 0.15077 20200.00 15200.00 0.00 0.15175 20000.00 15400.00 0.00 0.15421 20200.00 15400.00 0.00 0.15510 20000.00 15600.00 0.00 0.15756 20200.00 15600.00 0.00 0.15838 20000.00 15800.00 0.00 0.16072 20200.00 15800.00 0.00 0.16147 20000.00 16000.00 0.00 0.16380 20200.00 16000.00 0.00 0.16447 19800.00 16200.00 0.00 0.16482 20000.00 16200.00 0.00 0.16713 20200.00 16200.00 0.00 0.16773 19800.00 16400.00 0.00 0.16856 20000.00 16400.00 0.00 0.17082 20200.00 16400.00 0.00 0.17133 19800.00 16600.00 0.00 0.17245 20000.00 16600.00 0.00 0.17465 20200.00 16600.00 0.00 0.17507 19800.00 16800.00 0.00 0.17635 20000.00 16800.00 0.00 0.17847 20200.00 16800.00 0.00 0.17879 19800.00 17000.00 0.00 0.18011 20000.00 17000.00 0.00 0.18216 20200.00 17000.00 0.00 0.18237 19800.00 17200.00 0.00 0.18370 20000.00 17200.00 0.00 0.18567 20200.00 17200.00 0.00 0.18575 19800.00 17400.00 0.00 0.18741 20000.00 17400.00 0.00 0.18928 19800.00 17600.00 0.00 0.19147 20000.00 17600.00 0.00 0.19322 19800.00 17800.00 0.00 0.19572 20000.00 17800.00 0.00 0.19730 19800.00 18000.00 0.00 0.19991 20000.00 18000.00 0.00 0.20131 19800.00 18200.00 0.00 0.20390 20000.00 18200.00 0.00 0.20510 19800.00 18400.00 0.00 0.20761 20000.00 18400.00 0.00 0.20859 19800.00 18600.00 0.00 0.21137 20000.00 18600.00 0.00 0.21209 19600.00 18800.00 0.00 0.21253 19800.00 18800.00 0.00 0.21569 20000.00 18800.00 0.00 0.21606 19600.00 19000.00 0.00 0.21766 19800.00 19000.00 0.00 0.22045 19600.00 19200.00 0.00 0.22278 19800.00 19200.00 0.00 0.22501 19600.00 19400.00 0.00 0.22722 19800.00 19400.00 0.00 0.22871 19600.00 19600.00 0.00 0.23005 19800.00 19600.00 0.00 0.23063 19600.00 19800.00 10.00 0.23074 19600.00 19800.00 15.00 0.23086 19600.00 19800.00 20.00 0.23097 19600.00 19800.00 25.00 0.23107 19600.00 19800.00 30.00 0.23116 19600.00 19800.00 35.00 0.23123 19600.00 19800.00 40.00 0.23128 19600.00 19800.00 45.00 0.23130 19600.00 19800.00 50.00 0.23130 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 19621.55 19783.10 47.81 0.23133 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/049.ctf NX, NY, NZ: 360 360 1 MODE : real