CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/050.mrc Output diagnostic file name Micrographs/050.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/050.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2148.000 8096.000 Mean, RMS : 4402.029 230.8577 TITLE 1: emi2mrc: 050.emi Sun Jan 19 14:45:19 2014 TITLE 2: kV 300, Mag 78000x, Def 25920, Spot 4, Int 55.14%, Obj 88.04%, Dif 73.67% TITLE 3: X 281.901, Y -464.386, Z -58.220, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 119 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.03586 15200.00 8000.00 0.00 -0.03541 15400.00 8000.00 0.00 -0.03471 15600.00 8000.00 0.00 -0.03384 15800.00 8000.00 0.00 -0.03278 16000.00 8000.00 0.00 -0.03154 16200.00 8000.00 0.00 -0.03026 16400.00 8000.00 0.00 -0.02907 16600.00 8000.00 0.00 -0.02802 16800.00 8000.00 0.00 -0.02708 17000.00 8000.00 0.00 -0.02612 17200.00 8000.00 0.00 -0.02503 17400.00 8000.00 0.00 -0.02372 17600.00 8000.00 0.00 -0.02220 17800.00 8000.00 0.00 -0.02051 18000.00 8000.00 0.00 -0.01871 18200.00 8000.00 0.00 -0.01689 18400.00 8000.00 0.00 -0.01508 18600.00 8000.00 0.00 -0.01333 18800.00 8000.00 0.00 -0.01164 19000.00 8000.00 0.00 -0.00999 19200.00 8000.00 0.00 -0.00834 19400.00 8000.00 0.00 -0.00665 19600.00 8000.00 0.00 -0.00491 19800.00 8000.00 0.00 -0.00313 20000.00 8000.00 0.00 -0.00134 20200.00 8000.00 0.00 0.00043 20400.00 8000.00 0.00 0.00218 20600.00 8000.00 0.00 0.00394 20800.00 8000.00 0.00 0.00571 21000.00 8000.00 0.00 0.00750 21200.00 8000.00 0.00 0.00930 21400.00 8000.00 0.00 0.01110 21600.00 8000.00 0.00 0.01293 21800.00 8000.00 0.00 0.01485 22000.00 8000.00 0.00 0.01694 22200.00 8000.00 0.00 0.01927 22400.00 8000.00 0.00 0.02185 22600.00 8000.00 0.00 0.02468 22800.00 8000.00 0.00 0.02770 23000.00 8000.00 0.00 0.03084 23200.00 8000.00 0.00 0.03403 23400.00 8000.00 0.00 0.03719 23600.00 8000.00 0.00 0.04019 23800.00 8000.00 0.00 0.04291 24000.00 8000.00 0.00 0.04522 24200.00 8000.00 0.00 0.04701 24400.00 8000.00 0.00 0.04823 24600.00 8000.00 0.00 0.04895 24800.00 8000.00 0.00 0.04923 25000.00 8000.00 0.00 0.04924 24800.00 8200.00 0.00 0.04949 24800.00 8400.00 0.00 0.04965 24600.00 8600.00 0.00 0.04966 24800.00 8600.00 0.00 0.04987 24600.00 8800.00 0.00 0.05001 24800.00 8800.00 0.00 0.05019 24600.00 9000.00 0.00 0.05045 24800.00 9000.00 0.00 0.05060 24600.00 9200.00 0.00 0.05095 24800.00 9200.00 0.00 0.05106 24600.00 9400.00 0.00 0.05143 24800.00 9400.00 0.00 0.05151 24600.00 9600.00 0.00 0.05188 24800.00 9600.00 0.00 0.05193 24600.00 9800.00 0.00 0.05239 24800.00 9800.00 0.00 0.05243 24400.00 10000.00 0.00 0.05251 24600.00 10000.00 0.00 0.05306 24800.00 10000.00 0.00 0.05309 24400.00 10200.00 0.00 0.05334 24600.00 10200.00 0.00 0.05387 24800.00 10200.00 0.00 0.05390 24400.00 10400.00 0.00 0.05428 24600.00 10400.00 0.00 0.05480 24800.00 10400.00 0.00 0.05481 24400.00 10600.00 0.00 0.05525 24600.00 10600.00 0.00 0.05574 24400.00 10800.00 0.00 0.05615 24600.00 10800.00 0.00 0.05661 24400.00 11000.00 0.00 0.05699 24600.00 11000.00 0.00 0.05743 24400.00 11200.00 0.00 0.05781 24600.00 11200.00 0.00 0.05823 24400.00 11400.00 0.00 0.05863 24600.00 11400.00 0.00 0.05904 24400.00 11600.00 0.00 0.05944 24600.00 11600.00 0.00 0.05985 24400.00 11800.00 0.00 0.06024 24600.00 11800.00 0.00 0.06068 24800.00 11800.00 0.00 0.06068 24400.00 12000.00 0.00 0.06100 24600.00 12000.00 0.00 0.06147 24800.00 12000.00 0.00 0.06150 24400.00 12200.00 0.00 0.06176 24600.00 12200.00 0.00 0.06225 24800.00 12200.00 0.00 0.06231 24400.00 12400.00 0.00 0.06261 24600.00 12400.00 0.00 0.06313 24800.00 12400.00 0.00 0.06321 24400.00 12600.00 0.00 0.06361 24600.00 12600.00 0.00 0.06415 24800.00 12600.00 0.00 0.06423 24400.00 12800.00 0.00 0.06474 24600.00 12800.00 0.00 0.06528 24800.00 12800.00 0.00 0.06535 24400.00 13000.00 0.00 0.06597 24600.00 13000.00 0.00 0.06651 24800.00 13000.00 0.00 0.06658 24400.00 13200.00 0.00 0.06724 24600.00 13200.00 0.00 0.06777 24800.00 13200.00 0.00 0.06782 24400.00 13400.00 0.00 0.06847 24600.00 13400.00 0.00 0.06899 24800.00 13400.00 0.00 0.06900 24400.00 13600.00 0.00 0.06970 24600.00 13600.00 0.00 0.07020 24400.00 13800.00 0.00 0.07099 24600.00 13800.00 0.00 0.07148 24400.00 14000.00 0.00 0.07236 24600.00 14000.00 0.00 0.07284 24400.00 14200.00 0.00 0.07383 24600.00 14200.00 0.00 0.07431 24400.00 14400.00 0.00 0.07538 24600.00 14400.00 0.00 0.07585 24400.00 14600.00 0.00 0.07692 24600.00 14600.00 0.00 0.07738 24400.00 14800.00 0.00 0.07845 24600.00 14800.00 0.00 0.07890 24400.00 15000.00 0.00 0.08001 24600.00 15000.00 0.00 0.08046 24400.00 15200.00 0.00 0.08159 24600.00 15200.00 0.00 0.08205 24200.00 15400.00 0.00 0.08206 24400.00 15400.00 0.00 0.08323 24600.00 15400.00 0.00 0.08368 24200.00 15600.00 0.00 0.08378 24400.00 15600.00 0.00 0.08495 24600.00 15600.00 0.00 0.08541 24200.00 15800.00 0.00 0.08557 24400.00 15800.00 0.00 0.08675 24600.00 15800.00 0.00 0.08720 24200.00 16000.00 0.00 0.08743 24400.00 16000.00 0.00 0.08861 24600.00 16000.00 0.00 0.08904 24200.00 16200.00 0.00 0.08939 24400.00 16200.00 0.00 0.09057 24600.00 16200.00 0.00 0.09099 24200.00 16400.00 0.00 0.09143 24400.00 16400.00 0.00 0.09260 24600.00 16400.00 0.00 0.09300 24200.00 16600.00 0.00 0.09348 24400.00 16600.00 0.00 0.09465 24600.00 16600.00 0.00 0.09503 24200.00 16800.00 0.00 0.09557 24400.00 16800.00 0.00 0.09674 24600.00 16800.00 0.00 0.09709 24200.00 17000.00 0.00 0.09769 24400.00 17000.00 0.00 0.09885 24600.00 17000.00 0.00 0.09918 24200.00 17200.00 0.00 0.09983 24400.00 17200.00 0.00 0.10098 24600.00 17200.00 0.00 0.10128 24200.00 17400.00 0.00 0.10207 24400.00 17400.00 0.00 0.10321 24600.00 17400.00 0.00 0.10348 24200.00 17600.00 0.00 0.10444 24400.00 17600.00 0.00 0.10556 24600.00 17600.00 0.00 0.10577 24200.00 17800.00 0.00 0.10683 24400.00 17800.00 0.00 0.10791 24600.00 17800.00 0.00 0.10806 24200.00 18000.00 0.00 0.10919 24400.00 18000.00 0.00 0.11022 24600.00 18000.00 0.00 0.11031 24200.00 18200.00 0.00 0.11150 24400.00 18200.00 0.00 0.11249 24600.00 18200.00 0.00 0.11253 24200.00 18400.00 0.00 0.11374 24400.00 18400.00 0.00 0.11470 24200.00 18600.00 0.00 0.11599 24400.00 18600.00 0.00 0.11692 24200.00 18800.00 0.00 0.11839 24400.00 18800.00 0.00 0.11927 24200.00 19000.00 0.00 0.12089 24400.00 19000.00 0.00 0.12172 24200.00 19200.00 0.00 0.12345 24400.00 19200.00 0.00 0.12422 24200.00 19400.00 0.00 0.12608 24400.00 19400.00 0.00 0.12680 24000.00 19600.00 0.00 0.12683 24200.00 19600.00 0.00 0.12871 24400.00 19600.00 0.00 0.12937 24000.00 19800.00 0.00 0.12947 24200.00 19800.00 0.00 0.13131 24400.00 19800.00 0.00 0.13190 24000.00 20000.00 0.00 0.13218 24200.00 20000.00 0.00 0.13397 24400.00 20000.00 0.00 0.13448 24000.00 20200.00 0.00 0.13494 24200.00 20200.00 0.00 0.13667 24400.00 20200.00 0.00 0.13710 24000.00 20400.00 0.00 0.13762 24200.00 20400.00 0.00 0.13929 24400.00 20400.00 0.00 0.13963 24000.00 20600.00 0.00 0.14025 24200.00 20600.00 0.00 0.14185 24400.00 20600.00 0.00 0.14210 24000.00 20800.00 0.00 0.14289 24200.00 20800.00 0.00 0.14442 24400.00 20800.00 0.00 0.14458 24000.00 21000.00 0.00 0.14557 24200.00 21000.00 0.00 0.14703 24400.00 21000.00 0.00 0.14708 24000.00 21200.00 0.00 0.14844 24200.00 21200.00 0.00 0.14981 24000.00 21400.00 0.00 0.15159 24200.00 21400.00 0.00 0.15286 24000.00 21600.00 0.00 0.15481 24200.00 21600.00 0.00 0.15595 24000.00 21800.00 0.00 0.15793 24200.00 21800.00 0.00 0.15891 24000.00 22000.00 0.00 0.16087 24200.00 22000.00 0.00 0.16170 24000.00 22200.00 0.00 0.16359 24200.00 22200.00 0.00 0.16427 24000.00 22400.00 0.00 0.16624 24200.00 22400.00 0.00 0.16675 23800.00 22600.00 0.00 0.16713 24000.00 22600.00 0.00 0.16912 24200.00 22600.00 0.00 0.16942 23800.00 22800.00 0.00 0.17038 24000.00 22800.00 0.00 0.17216 24200.00 22800.00 0.00 0.17221 23800.00 23000.00 0.00 0.17360 24000.00 23000.00 0.00 0.17511 23800.00 23200.00 0.00 0.17661 24000.00 23200.00 0.00 0.17779 23800.00 23400.00 0.00 0.17903 24000.00 23400.00 0.00 0.17985 23800.00 23600.00 0.00 0.18053 24000.00 23600.00 0.00 0.18100 23800.00 23800.00 0.00 0.18129 24000.00 23800.00 0.00 0.18143 23800.00 24000.00 0.00 0.18146 23800.00 24000.00 5.00 0.18153 23800.00 24000.00 10.00 0.18160 23800.00 24000.00 15.00 0.18166 23800.00 24000.00 20.00 0.18172 23800.00 24000.00 25.00 0.18177 23800.00 24000.00 30.00 0.18181 23800.00 24000.00 35.00 0.18184 23800.00 24000.00 40.00 0.18185 23800.00 24000.00 45.00 0.18185 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 23759.37 23992.12 45.00 0.18188 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/050.ctf NX, NY, NZ: 360 360 1 MODE : real