CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/054.mrc Output diagnostic file name Micrographs/054.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/054.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2388.000 7600.000 Mean, RMS : 4411.238 227.0811 TITLE 1: emi2mrc: 054.emi Sun Jan 19 14:48:19 2014 TITLE 2: kV 300, Mag 78000x, Def 25920, Spot 4, Int 55.14%, Obj 88.04%, Dif 73.67% TITLE 3: X 280.249, Y -463.015, Z -58.220, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.03880 16800.00 8000.00 0.00 -0.03838 17000.00 8000.00 0.00 -0.03761 17200.00 8000.00 0.00 -0.03677 17400.00 8000.00 0.00 -0.03582 17600.00 8000.00 0.00 -0.03476 17800.00 8000.00 0.00 -0.03358 18000.00 8000.00 0.00 -0.03225 18200.00 8000.00 0.00 -0.03082 18400.00 8000.00 0.00 -0.02934 18600.00 8000.00 0.00 -0.02783 18800.00 8000.00 0.00 -0.02634 19000.00 8000.00 0.00 -0.02484 19200.00 8000.00 0.00 -0.02331 19400.00 8000.00 0.00 -0.02176 19600.00 8000.00 0.00 -0.02023 19800.00 8000.00 0.00 -0.01877 20000.00 8000.00 0.00 -0.01741 20200.00 8000.00 0.00 -0.01610 20400.00 8000.00 0.00 -0.01471 20600.00 8000.00 0.00 -0.01315 20800.00 8000.00 0.00 -0.01140 21000.00 8000.00 0.00 -0.00954 21200.00 8000.00 0.00 -0.00773 21400.00 8000.00 0.00 -0.00608 21600.00 8000.00 0.00 -0.00463 21800.00 8000.00 0.00 -0.00330 22000.00 8000.00 0.00 -0.00196 22200.00 8000.00 0.00 -0.00052 22400.00 8000.00 0.00 0.00109 22600.00 8000.00 0.00 0.00286 22800.00 8000.00 0.00 0.00477 23000.00 8000.00 0.00 0.00681 23200.00 8000.00 0.00 0.00896 23400.00 8000.00 0.00 0.01119 23600.00 8000.00 0.00 0.01346 23800.00 8000.00 0.00 0.01576 24000.00 8000.00 0.00 0.01816 24200.00 8000.00 0.00 0.02077 24400.00 8000.00 0.00 0.02367 24600.00 8000.00 0.00 0.02684 24800.00 8000.00 0.00 0.03007 25000.00 8000.00 0.00 0.03303 25200.00 8000.00 0.00 0.03539 25400.00 8000.00 0.00 0.03694 25600.00 8000.00 0.00 0.03769 25800.00 8000.00 0.00 0.03787 25800.00 8200.00 0.00 0.03796 25800.00 8400.00 0.00 0.03798 25800.00 8600.00 0.00 0.03803 25600.00 8800.00 0.00 0.03806 25800.00 8800.00 0.00 0.03816 25600.00 9000.00 0.00 0.03830 25800.00 9000.00 0.00 0.03840 25600.00 9200.00 0.00 0.03865 25800.00 9200.00 0.00 0.03875 25600.00 9400.00 0.00 0.03906 25800.00 9400.00 0.00 0.03917 25600.00 9600.00 0.00 0.03948 25800.00 9600.00 0.00 0.03960 25600.00 9800.00 0.00 0.03991 25800.00 9800.00 0.00 0.04002 25600.00 10000.00 0.00 0.04036 25800.00 10000.00 0.00 0.04049 25600.00 10200.00 0.00 0.04087 25800.00 10200.00 0.00 0.04101 25600.00 10400.00 0.00 0.04147 25800.00 10400.00 0.00 0.04163 25600.00 10600.00 0.00 0.04219 25800.00 10600.00 0.00 0.04237 25600.00 10800.00 0.00 0.04295 25800.00 10800.00 0.00 0.04314 25600.00 11000.00 0.00 0.04371 25800.00 11000.00 0.00 0.04391 25600.00 11200.00 0.00 0.04443 25800.00 11200.00 0.00 0.04464 25600.00 11400.00 0.00 0.04507 25800.00 11400.00 0.00 0.04528 25600.00 11600.00 0.00 0.04565 25800.00 11600.00 0.00 0.04585 25600.00 11800.00 0.00 0.04625 25800.00 11800.00 0.00 0.04644 25600.00 12000.00 0.00 0.04691 25800.00 12000.00 0.00 0.04711 25600.00 12200.00 0.00 0.04770 25800.00 12200.00 0.00 0.04790 25600.00 12400.00 0.00 0.04863 25800.00 12400.00 0.00 0.04882 25400.00 12600.00 0.00 0.04885 25600.00 12600.00 0.00 0.04962 25800.00 12600.00 0.00 0.04979 25400.00 12800.00 0.00 0.04983 25600.00 12800.00 0.00 0.05057 25800.00 12800.00 0.00 0.05072 25400.00 13000.00 0.00 0.05075 25600.00 13000.00 0.00 0.05148 25800.00 13000.00 0.00 0.05162 25400.00 13200.00 0.00 0.05166 25600.00 13200.00 0.00 0.05237 25800.00 13200.00 0.00 0.05251 25400.00 13400.00 0.00 0.05264 25600.00 13400.00 0.00 0.05335 25800.00 13400.00 0.00 0.05349 25400.00 13600.00 0.00 0.05382 25600.00 13600.00 0.00 0.05454 25800.00 13600.00 0.00 0.05469 25400.00 13800.00 0.00 0.05525 25600.00 13800.00 0.00 0.05598 25800.00 13800.00 0.00 0.05614 25400.00 14000.00 0.00 0.05681 25600.00 14000.00 0.00 0.05755 25800.00 14000.00 0.00 0.05771 25400.00 14200.00 0.00 0.05839 25600.00 14200.00 0.00 0.05913 25800.00 14200.00 0.00 0.05929 25400.00 14400.00 0.00 0.05985 25600.00 14400.00 0.00 0.06059 25800.00 14400.00 0.00 0.06073 25400.00 14600.00 0.00 0.06112 25600.00 14600.00 0.00 0.06185 25800.00 14600.00 0.00 0.06197 25400.00 14800.00 0.00 0.06226 25600.00 14800.00 0.00 0.06298 25800.00 14800.00 0.00 0.06306 25400.00 15000.00 0.00 0.06341 25600.00 15000.00 0.00 0.06411 25800.00 15000.00 0.00 0.06417 25400.00 15200.00 0.00 0.06465 25600.00 15200.00 0.00 0.06533 25800.00 15200.00 0.00 0.06535 25400.00 15400.00 0.00 0.06599 25600.00 15400.00 0.00 0.06664 25400.00 15600.00 0.00 0.06741 25600.00 15600.00 0.00 0.06803 25400.00 15800.00 0.00 0.06884 25600.00 15800.00 0.00 0.06943 25400.00 16000.00 0.00 0.07025 25600.00 16000.00 0.00 0.07081 25400.00 16200.00 0.00 0.07170 25600.00 16200.00 0.00 0.07225 25400.00 16400.00 0.00 0.07326 25600.00 16400.00 0.00 0.07381 25400.00 16600.00 0.00 0.07495 25600.00 16600.00 0.00 0.07551 25400.00 16800.00 0.00 0.07679 25600.00 16800.00 0.00 0.07736 25400.00 17000.00 0.00 0.07873 25600.00 17000.00 0.00 0.07930 25400.00 17200.00 0.00 0.08070 25600.00 17200.00 0.00 0.08126 25400.00 17400.00 0.00 0.08269 25600.00 17400.00 0.00 0.08323 25400.00 17600.00 0.00 0.08471 25600.00 17600.00 0.00 0.08522 25200.00 17800.00 0.00 0.08525 25400.00 17800.00 0.00 0.08674 25600.00 17800.00 0.00 0.08720 25200.00 18000.00 0.00 0.08733 25400.00 18000.00 0.00 0.08878 25600.00 18000.00 0.00 0.08918 25200.00 18200.00 0.00 0.08946 25400.00 18200.00 0.00 0.09085 25600.00 18200.00 0.00 0.09121 25200.00 18400.00 0.00 0.09158 25400.00 18400.00 0.00 0.09293 25600.00 18400.00 0.00 0.09326 25200.00 18600.00 0.00 0.09375 25400.00 18600.00 0.00 0.09508 25600.00 18600.00 0.00 0.09538 25200.00 18800.00 0.00 0.09604 25400.00 18800.00 0.00 0.09737 25600.00 18800.00 0.00 0.09767 25200.00 19000.00 0.00 0.09842 25400.00 19000.00 0.00 0.09976 25600.00 19000.00 0.00 0.10008 25200.00 19200.00 0.00 0.10085 25400.00 19200.00 0.00 0.10221 25600.00 19200.00 0.00 0.10254 25200.00 19400.00 0.00 0.10334 25400.00 19400.00 0.00 0.10472 25600.00 19400.00 0.00 0.10506 25200.00 19600.00 0.00 0.10585 25400.00 19600.00 0.00 0.10724 25600.00 19600.00 0.00 0.10757 25200.00 19800.00 0.00 0.10838 25400.00 19800.00 0.00 0.10977 25600.00 19800.00 0.00 0.11008 25200.00 20000.00 0.00 0.11100 25400.00 20000.00 0.00 0.11237 25600.00 20000.00 0.00 0.11264 25200.00 20200.00 0.00 0.11368 25400.00 20200.00 0.00 0.11501 25600.00 20200.00 0.00 0.11523 25200.00 20400.00 0.00 0.11628 25400.00 20400.00 0.00 0.11758 25600.00 20400.00 0.00 0.11774 25200.00 20600.00 0.00 0.11880 25400.00 20600.00 0.00 0.12008 25600.00 20600.00 0.00 0.12021 25200.00 20800.00 0.00 0.12126 25400.00 20800.00 0.00 0.12254 25600.00 20800.00 0.00 0.12265 25200.00 21000.00 0.00 0.12367 25400.00 21000.00 0.00 0.12497 25600.00 21000.00 0.00 0.12508 25200.00 21200.00 0.00 0.12622 25400.00 21200.00 0.00 0.12754 25600.00 21200.00 0.00 0.12763 25200.00 21400.00 0.00 0.12905 25400.00 21400.00 0.00 0.13038 25600.00 21400.00 0.00 0.13043 25200.00 21600.00 0.00 0.13206 25400.00 21600.00 0.00 0.13338 25200.00 21800.00 0.00 0.13515 25400.00 21800.00 0.00 0.13643 25200.00 22000.00 0.00 0.13831 25400.00 22000.00 0.00 0.13953 25200.00 22200.00 0.00 0.14146 25400.00 22200.00 0.00 0.14261 25200.00 22400.00 0.00 0.14467 25400.00 22400.00 0.00 0.14572 25200.00 22600.00 0.00 0.14815 25400.00 22600.00 0.00 0.14907 25000.00 22800.00 0.00 0.14932 25200.00 22800.00 0.00 0.15186 25400.00 22800.00 0.00 0.15262 25000.00 23000.00 0.00 0.15322 25200.00 23000.00 0.00 0.15562 25400.00 23000.00 0.00 0.15620 25000.00 23200.00 0.00 0.15712 25200.00 23200.00 0.00 0.15936 25400.00 23200.00 0.00 0.15973 25000.00 23400.00 0.00 0.16086 25200.00 23400.00 0.00 0.16294 25400.00 23400.00 0.00 0.16309 25000.00 23600.00 0.00 0.16443 25200.00 23600.00 0.00 0.16633 25000.00 23800.00 0.00 0.16810 25200.00 23800.00 0.00 0.16980 25000.00 24000.00 0.00 0.17200 25200.00 24000.00 0.00 0.17342 25000.00 24200.00 0.00 0.17589 25200.00 24200.00 0.00 0.17695 25000.00 24400.00 0.00 0.17956 25200.00 24400.00 0.00 0.18018 24800.00 24600.00 0.00 0.18027 25000.00 24600.00 0.00 0.18259 25200.00 24600.00 0.00 0.18268 25000.00 24800.00 0.00 0.18433 25000.00 25000.00 0.00 0.18465 24800.00 25200.00 30.00 0.18482 24800.00 25200.00 35.00 0.18501 24800.00 25200.00 40.00 0.18515 24800.00 25200.00 45.00 0.18524 24800.00 25200.00 50.00 0.18528 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 24836.70 25145.41 51.73 0.18534 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/054.ctf NX, NY, NZ: 360 360 1 MODE : real