CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/067.mrc Output diagnostic file name Micrographs/067.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/067.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2348.000 8032.000 Mean, RMS : 4407.655 224.9902 TITLE 1: emi2mrc: 067.emi Sun Jan 19 14:59:54 2014 TITLE 2: kV 300, Mag 78000x, Def 25920, Spot 4, Int 55.14%, Obj 88.04%, Dif 73.67% TITLE 3: X 274.992, Y -463.532, Z -58.220, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.03735 15600.00 8000.00 0.00 -0.03723 15800.00 8000.00 0.00 -0.03620 16000.00 8000.00 0.00 -0.03509 16200.00 8000.00 0.00 -0.03401 16400.00 8000.00 0.00 -0.03306 16600.00 8000.00 0.00 -0.03222 16800.00 8000.00 0.00 -0.03140 17000.00 8000.00 0.00 -0.03049 17200.00 8000.00 0.00 -0.02938 17400.00 8000.00 0.00 -0.02807 17600.00 8000.00 0.00 -0.02664 17800.00 8000.00 0.00 -0.02517 18000.00 8000.00 0.00 -0.02370 18200.00 8000.00 0.00 -0.02220 18400.00 8000.00 0.00 -0.02064 18600.00 8000.00 0.00 -0.01900 18800.00 8000.00 0.00 -0.01731 19000.00 8000.00 0.00 -0.01562 19200.00 8000.00 0.00 -0.01398 19400.00 8000.00 0.00 -0.01237 19600.00 8000.00 0.00 -0.01072 19800.00 8000.00 0.00 -0.00897 20000.00 8000.00 0.00 -0.00711 20200.00 8000.00 0.00 -0.00519 20400.00 8000.00 0.00 -0.00332 20600.00 8000.00 0.00 -0.00159 20800.00 8000.00 0.00 -0.00001 21000.00 8000.00 0.00 0.00146 21200.00 8000.00 0.00 0.00290 21400.00 8000.00 0.00 0.00441 21600.00 8000.00 0.00 0.00606 21800.00 8000.00 0.00 0.00787 22000.00 8000.00 0.00 0.00988 22200.00 8000.00 0.00 0.01213 22400.00 8000.00 0.00 0.01458 22600.00 8000.00 0.00 0.01718 22800.00 8000.00 0.00 0.01984 23000.00 8000.00 0.00 0.02245 23200.00 8000.00 0.00 0.02500 23400.00 8000.00 0.00 0.02756 23600.00 8000.00 0.00 0.03027 23800.00 8000.00 0.00 0.03322 24000.00 8000.00 0.00 0.03637 24200.00 8000.00 0.00 0.03948 24400.00 8000.00 0.00 0.04220 24600.00 8000.00 0.00 0.04425 24800.00 8000.00 0.00 0.04548 25000.00 8000.00 0.00 0.04599 25200.00 8000.00 0.00 0.04603 25000.00 8200.00 0.00 0.04646 25200.00 8200.00 0.00 0.04651 25000.00 8400.00 0.00 0.04696 25200.00 8400.00 0.00 0.04702 24800.00 8600.00 0.00 0.04704 25000.00 8600.00 0.00 0.04755 25200.00 8600.00 0.00 0.04763 24800.00 8800.00 0.00 0.04768 25000.00 8800.00 0.00 0.04820 25200.00 8800.00 0.00 0.04831 25000.00 9000.00 0.00 0.04882 25200.00 9000.00 0.00 0.04896 25000.00 9200.00 0.00 0.04937 25200.00 9200.00 0.00 0.04953 25000.00 9400.00 0.00 0.04985 25200.00 9400.00 0.00 0.05003 25000.00 9600.00 0.00 0.05032 25200.00 9600.00 0.00 0.05052 25400.00 9600.00 0.00 0.05052 25000.00 9800.00 0.00 0.05090 25200.00 9800.00 0.00 0.05110 25000.00 10000.00 0.00 0.05161 25200.00 10000.00 0.00 0.05181 25000.00 10200.00 0.00 0.05234 25200.00 10200.00 0.00 0.05253 25000.00 10400.00 0.00 0.05301 25200.00 10400.00 0.00 0.05319 25000.00 10600.00 0.00 0.05356 25200.00 10600.00 0.00 0.05372 25000.00 10800.00 0.00 0.05402 25200.00 10800.00 0.00 0.05418 25000.00 11000.00 0.00 0.05454 25200.00 11000.00 0.00 0.05470 25000.00 11200.00 0.00 0.05525 25200.00 11200.00 0.00 0.05540 24800.00 11400.00 0.00 0.05554 25000.00 11400.00 0.00 0.05614 25200.00 11400.00 0.00 0.05631 24800.00 11600.00 0.00 0.05653 25000.00 11600.00 0.00 0.05714 25200.00 11600.00 0.00 0.05732 24800.00 11800.00 0.00 0.05753 25000.00 11800.00 0.00 0.05815 25200.00 11800.00 0.00 0.05834 24800.00 12000.00 0.00 0.05846 25000.00 12000.00 0.00 0.05909 25200.00 12000.00 0.00 0.05928 24800.00 12200.00 0.00 0.05932 25000.00 12200.00 0.00 0.05997 25200.00 12200.00 0.00 0.06017 24800.00 12400.00 0.00 0.06025 25000.00 12400.00 0.00 0.06090 25200.00 12400.00 0.00 0.06111 24800.00 12600.00 0.00 0.06129 25000.00 12600.00 0.00 0.06195 25200.00 12600.00 0.00 0.06216 24800.00 12800.00 0.00 0.06243 25000.00 12800.00 0.00 0.06309 25200.00 12800.00 0.00 0.06330 24800.00 13000.00 0.00 0.06364 25000.00 13000.00 0.00 0.06431 25200.00 13000.00 0.00 0.06450 24800.00 13200.00 0.00 0.06488 25000.00 13200.00 0.00 0.06555 25200.00 13200.00 0.00 0.06571 24800.00 13400.00 0.00 0.06611 25000.00 13400.00 0.00 0.06676 25200.00 13400.00 0.00 0.06691 24800.00 13600.00 0.00 0.06738 25000.00 13600.00 0.00 0.06803 25200.00 13600.00 0.00 0.06816 24800.00 13800.00 0.00 0.06876 25000.00 13800.00 0.00 0.06941 25200.00 13800.00 0.00 0.06953 24800.00 14000.00 0.00 0.07024 25000.00 14000.00 0.00 0.07088 25200.00 14000.00 0.00 0.07097 24800.00 14200.00 0.00 0.07177 25000.00 14200.00 0.00 0.07240 25200.00 14200.00 0.00 0.07246 24800.00 14400.00 0.00 0.07336 25000.00 14400.00 0.00 0.07396 25200.00 14400.00 0.00 0.07399 24800.00 14600.00 0.00 0.07494 25000.00 14600.00 0.00 0.07551 24800.00 14800.00 0.00 0.07653 25000.00 14800.00 0.00 0.07706 24800.00 15000.00 0.00 0.07815 25000.00 15000.00 0.00 0.07864 24800.00 15200.00 0.00 0.07974 25000.00 15200.00 0.00 0.08019 24800.00 15400.00 0.00 0.08124 25000.00 15400.00 0.00 0.08164 24800.00 15600.00 0.00 0.08265 25000.00 15600.00 0.00 0.08302 24800.00 15800.00 0.00 0.08401 25000.00 15800.00 0.00 0.08437 24800.00 16000.00 0.00 0.08544 25000.00 16000.00 0.00 0.08578 24600.00 16200.00 0.00 0.08580 24800.00 16200.00 0.00 0.08705 25000.00 16200.00 0.00 0.08736 24600.00 16400.00 0.00 0.08762 24800.00 16400.00 0.00 0.08884 25000.00 16400.00 0.00 0.08913 24600.00 16600.00 0.00 0.08953 24800.00 16600.00 0.00 0.09072 25000.00 16600.00 0.00 0.09098 24600.00 16800.00 0.00 0.09147 24800.00 16800.00 0.00 0.09264 25000.00 16800.00 0.00 0.09286 24600.00 17000.00 0.00 0.09339 24800.00 17000.00 0.00 0.09454 25000.00 17000.00 0.00 0.09474 24600.00 17200.00 0.00 0.09529 24800.00 17200.00 0.00 0.09642 25000.00 17200.00 0.00 0.09661 24600.00 17400.00 0.00 0.09730 24800.00 17400.00 0.00 0.09843 25000.00 17400.00 0.00 0.09861 24600.00 17600.00 0.00 0.09950 24800.00 17600.00 0.00 0.10062 25000.00 17600.00 0.00 0.10080 24600.00 17800.00 0.00 0.10180 24800.00 17800.00 0.00 0.10292 25000.00 17800.00 0.00 0.10309 24600.00 18000.00 0.00 0.10413 24800.00 18000.00 0.00 0.10525 25000.00 18000.00 0.00 0.10541 24600.00 18200.00 0.00 0.10646 24800.00 18200.00 0.00 0.10757 25000.00 18200.00 0.00 0.10771 24600.00 18400.00 0.00 0.10874 24800.00 18400.00 0.00 0.10983 25000.00 18400.00 0.00 0.10992 24600.00 18600.00 0.00 0.11104 24800.00 18600.00 0.00 0.11210 25000.00 18600.00 0.00 0.11213 24600.00 18800.00 0.00 0.11350 24800.00 18800.00 0.00 0.11452 24600.00 19000.00 0.00 0.11609 24800.00 19000.00 0.00 0.11707 24600.00 19200.00 0.00 0.11869 24800.00 19200.00 0.00 0.11964 24600.00 19400.00 0.00 0.12126 24800.00 19400.00 0.00 0.12217 24600.00 19600.00 0.00 0.12367 24800.00 19600.00 0.00 0.12453 24600.00 19800.00 0.00 0.12592 24800.00 19800.00 0.00 0.12674 24600.00 20000.00 0.00 0.12819 24800.00 20000.00 0.00 0.12896 24600.00 20200.00 0.00 0.13056 24800.00 20200.00 0.00 0.13127 24600.00 20400.00 0.00 0.13296 24800.00 20400.00 0.00 0.13361 24600.00 20600.00 0.00 0.13539 24800.00 20600.00 0.00 0.13596 24600.00 20800.00 0.00 0.13781 24800.00 20800.00 0.00 0.13830 24600.00 21000.00 0.00 0.14014 24800.00 21000.00 0.00 0.14053 24400.00 21200.00 0.00 0.14067 24600.00 21200.00 0.00 0.14247 24800.00 21200.00 0.00 0.14275 24400.00 21400.00 0.00 0.14317 24600.00 21400.00 0.00 0.14490 24800.00 21400.00 0.00 0.14508 24400.00 21600.00 0.00 0.14568 24600.00 21600.00 0.00 0.14734 24800.00 21600.00 0.00 0.14741 24400.00 21800.00 0.00 0.14816 24600.00 21800.00 0.00 0.14976 24400.00 22000.00 0.00 0.15071 24600.00 22000.00 0.00 0.15224 24400.00 22200.00 0.00 0.15332 24600.00 22200.00 0.00 0.15478 24400.00 22400.00 0.00 0.15607 24600.00 22400.00 0.00 0.15746 24400.00 22600.00 0.00 0.15912 24600.00 22600.00 0.00 0.16041 24400.00 22800.00 0.00 0.16229 24600.00 22800.00 0.00 0.16346 24400.00 23000.00 0.00 0.16532 24600.00 23000.00 0.00 0.16635 24400.00 23200.00 0.00 0.16816 24600.00 23200.00 0.00 0.16900 24400.00 23400.00 0.00 0.17071 24600.00 23400.00 0.00 0.17132 24400.00 23600.00 0.00 0.17297 24600.00 23600.00 0.00 0.17332 24400.00 23800.00 0.00 0.17524 24600.00 23800.00 0.00 0.17528 24200.00 24000.00 0.00 0.17573 24400.00 24000.00 0.00 0.17746 24200.00 24200.00 0.00 0.17773 24400.00 24200.00 0.00 0.17903 24400.00 24400.00 0.00 0.17957 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 24310.52 24451.35 40.19 0.17972 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/067.ctf NX, NY, NZ: 360 360 1 MODE : real