CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/071.mrc Output diagnostic file name Micrographs/071.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/071.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2014.000 7758.000 Mean, RMS : 4401.551 224.4163 TITLE 1: emi2mrc: 071.emi Sun Jan 19 15:07:08 2014 TITLE 2: kV 300, Mag 78000x, Def 23960, Spot 4, Int 55.14%, Obj 88.04%, Dif 73.67% TITLE 3: X 268.637, Y -465.989, Z -58.220, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.03284 15000.00 8000.00 0.00 -0.03204 15200.00 8000.00 0.00 -0.03118 15400.00 8000.00 0.00 -0.03036 15600.00 8000.00 0.00 -0.02954 15800.00 8000.00 0.00 -0.02869 16000.00 8000.00 0.00 -0.02776 16200.00 8000.00 0.00 -0.02674 16400.00 8000.00 0.00 -0.02564 16600.00 8000.00 0.00 -0.02443 16800.00 8000.00 0.00 -0.02306 17000.00 8000.00 0.00 -0.02152 17200.00 8000.00 0.00 -0.01981 17400.00 8000.00 0.00 -0.01798 17600.00 8000.00 0.00 -0.01609 17800.00 8000.00 0.00 -0.01417 18000.00 8000.00 0.00 -0.01222 18200.00 8000.00 0.00 -0.01022 18400.00 8000.00 0.00 -0.00815 18600.00 8000.00 0.00 -0.00601 18800.00 8000.00 0.00 -0.00389 19000.00 8000.00 0.00 -0.00187 19200.00 8000.00 0.00 -0.00002 19400.00 8000.00 0.00 0.00168 19600.00 8000.00 0.00 0.00328 19800.00 8000.00 0.00 0.00487 20000.00 8000.00 0.00 0.00655 20200.00 8000.00 0.00 0.00834 20400.00 8000.00 0.00 0.01022 20600.00 8000.00 0.00 0.01212 20800.00 8000.00 0.00 0.01401 21000.00 8000.00 0.00 0.01592 21200.00 8000.00 0.00 0.01790 21400.00 8000.00 0.00 0.02003 21600.00 8000.00 0.00 0.02231 21800.00 8000.00 0.00 0.02470 22000.00 8000.00 0.00 0.02711 22200.00 8000.00 0.00 0.02953 22400.00 8000.00 0.00 0.03197 22600.00 8000.00 0.00 0.03456 22800.00 8000.00 0.00 0.03738 23000.00 8000.00 0.00 0.04041 23200.00 8000.00 0.00 0.04350 23400.00 8000.00 0.00 0.04637 23600.00 8000.00 0.00 0.04871 23800.00 8000.00 0.00 0.05039 24000.00 8000.00 0.00 0.05142 24200.00 8000.00 0.00 0.05189 24400.00 8000.00 0.00 0.05201 24200.00 8200.00 0.00 0.05242 24400.00 8200.00 0.00 0.05254 24200.00 8400.00 0.00 0.05302 24400.00 8400.00 0.00 0.05315 24000.00 8600.00 0.00 0.05329 24200.00 8600.00 0.00 0.05379 24400.00 8600.00 0.00 0.05392 24000.00 8800.00 0.00 0.05417 24200.00 8800.00 0.00 0.05468 24400.00 8800.00 0.00 0.05480 24000.00 9000.00 0.00 0.05504 24200.00 9000.00 0.00 0.05555 24400.00 9000.00 0.00 0.05566 24000.00 9200.00 0.00 0.05581 24200.00 9200.00 0.00 0.05630 24400.00 9200.00 0.00 0.05641 24000.00 9400.00 0.00 0.05642 24200.00 9400.00 0.00 0.05690 24400.00 9400.00 0.00 0.05699 24200.00 9600.00 0.00 0.05739 24400.00 9600.00 0.00 0.05746 24000.00 9800.00 0.00 0.05746 24200.00 9800.00 0.00 0.05792 24400.00 9800.00 0.00 0.05798 24000.00 10000.00 0.00 0.05814 24200.00 10000.00 0.00 0.05859 24400.00 10000.00 0.00 0.05865 24000.00 10200.00 0.00 0.05894 24200.00 10200.00 0.00 0.05938 24400.00 10200.00 0.00 0.05944 24000.00 10400.00 0.00 0.05980 24200.00 10400.00 0.00 0.06023 24400.00 10400.00 0.00 0.06030 24000.00 10600.00 0.00 0.06067 24200.00 10600.00 0.00 0.06110 24400.00 10600.00 0.00 0.06115 24000.00 10800.00 0.00 0.06151 24200.00 10800.00 0.00 0.06193 24400.00 10800.00 0.00 0.06198 24000.00 11000.00 0.00 0.06239 24200.00 11000.00 0.00 0.06279 24400.00 11000.00 0.00 0.06284 24000.00 11200.00 0.00 0.06339 24200.00 11200.00 0.00 0.06378 24400.00 11200.00 0.00 0.06381 24000.00 11400.00 0.00 0.06448 24200.00 11400.00 0.00 0.06485 24400.00 11400.00 0.00 0.06486 24000.00 11600.00 0.00 0.06561 24200.00 11600.00 0.00 0.06596 24000.00 11800.00 0.00 0.06673 24200.00 11800.00 0.00 0.06707 24000.00 12000.00 0.00 0.06776 24200.00 12000.00 0.00 0.06809 24000.00 12200.00 0.00 0.06871 24200.00 12200.00 0.00 0.06904 24000.00 12400.00 0.00 0.06968 24200.00 12400.00 0.00 0.07002 24000.00 12600.00 0.00 0.07069 24200.00 12600.00 0.00 0.07105 24000.00 12800.00 0.00 0.07175 24200.00 12800.00 0.00 0.07212 24000.00 13000.00 0.00 0.07290 24200.00 13000.00 0.00 0.07328 24000.00 13200.00 0.00 0.07416 24200.00 13200.00 0.00 0.07454 24000.00 13400.00 0.00 0.07552 24200.00 13400.00 0.00 0.07589 23800.00 13600.00 0.00 0.07602 24000.00 13600.00 0.00 0.07705 24200.00 13600.00 0.00 0.07740 23800.00 13800.00 0.00 0.07773 24000.00 13800.00 0.00 0.07875 24200.00 13800.00 0.00 0.07907 23800.00 14000.00 0.00 0.07952 24000.00 14000.00 0.00 0.08053 24200.00 14000.00 0.00 0.08081 23800.00 14200.00 0.00 0.08136 24000.00 14200.00 0.00 0.08234 24200.00 14200.00 0.00 0.08258 23800.00 14400.00 0.00 0.08322 24000.00 14400.00 0.00 0.08417 24200.00 14400.00 0.00 0.08436 23800.00 14600.00 0.00 0.08509 24000.00 14600.00 0.00 0.08600 24200.00 14600.00 0.00 0.08613 23800.00 14800.00 0.00 0.08701 24000.00 14800.00 0.00 0.08786 24200.00 14800.00 0.00 0.08793 23800.00 15000.00 0.00 0.08899 24000.00 15000.00 0.00 0.08978 24200.00 15000.00 0.00 0.08979 23800.00 15200.00 0.00 0.09096 24000.00 15200.00 0.00 0.09170 23800.00 15400.00 0.00 0.09283 24000.00 15400.00 0.00 0.09354 23800.00 15600.00 0.00 0.09462 24000.00 15600.00 0.00 0.09532 23800.00 15800.00 0.00 0.09637 24000.00 15800.00 0.00 0.09706 23800.00 16000.00 0.00 0.09819 24000.00 16000.00 0.00 0.09890 23800.00 16200.00 0.00 0.10026 24000.00 16200.00 0.00 0.10098 23800.00 16400.00 0.00 0.10256 24000.00 16400.00 0.00 0.10329 23800.00 16600.00 0.00 0.10495 24000.00 16600.00 0.00 0.10568 23800.00 16800.00 0.00 0.10735 24000.00 16800.00 0.00 0.10805 23800.00 17000.00 0.00 0.10965 24000.00 17000.00 0.00 0.11030 23800.00 17200.00 0.00 0.11183 24000.00 17200.00 0.00 0.11241 23800.00 17400.00 0.00 0.11402 24000.00 17400.00 0.00 0.11454 23600.00 17600.00 0.00 0.11475 23800.00 17600.00 0.00 0.11632 24000.00 17600.00 0.00 0.11679 23600.00 17800.00 0.00 0.11714 23800.00 17800.00 0.00 0.11866 24000.00 17800.00 0.00 0.11907 23600.00 18000.00 0.00 0.11949 23800.00 18000.00 0.00 0.12098 24000.00 18000.00 0.00 0.12134 23600.00 18200.00 0.00 0.12181 23800.00 18200.00 0.00 0.12326 24000.00 18200.00 0.00 0.12357 23600.00 18400.00 0.00 0.12406 23800.00 18400.00 0.00 0.12548 24000.00 18400.00 0.00 0.12576 23600.00 18600.00 0.00 0.12639 23800.00 18600.00 0.00 0.12781 24000.00 18600.00 0.00 0.12807 23600.00 18800.00 0.00 0.12902 23800.00 18800.00 0.00 0.13042 24000.00 18800.00 0.00 0.13066 23600.00 19000.00 0.00 0.13186 23800.00 19000.00 0.00 0.13325 24000.00 19000.00 0.00 0.13345 23600.00 19200.00 0.00 0.13476 23800.00 19200.00 0.00 0.13613 24000.00 19200.00 0.00 0.13627 23600.00 19400.00 0.00 0.13765 23800.00 19400.00 0.00 0.13898 24000.00 19400.00 0.00 0.13906 23600.00 19600.00 0.00 0.14042 23800.00 19600.00 0.00 0.14168 23600.00 19800.00 0.00 0.14304 23800.00 19800.00 0.00 0.14423 23600.00 20000.00 0.00 0.14573 23800.00 20000.00 0.00 0.14684 23600.00 20200.00 0.00 0.14855 23800.00 20200.00 0.00 0.14958 23600.00 20400.00 0.00 0.15137 23800.00 20400.00 0.00 0.15231 23600.00 20600.00 0.00 0.15415 23800.00 20600.00 0.00 0.15500 23600.00 20800.00 0.00 0.15688 23800.00 20800.00 0.00 0.15765 23600.00 21000.00 0.00 0.15958 23800.00 21000.00 0.00 0.16027 23600.00 21200.00 0.00 0.16250 23800.00 21200.00 0.00 0.16310 23400.00 21400.00 0.00 0.16352 23600.00 21400.00 0.00 0.16582 23800.00 21400.00 0.00 0.16629 23400.00 21600.00 0.00 0.16715 23600.00 21600.00 0.00 0.16936 23800.00 21600.00 0.00 0.16967 23400.00 21800.00 0.00 0.17082 23600.00 21800.00 0.00 0.17291 23800.00 21800.00 0.00 0.17301 23400.00 22000.00 0.00 0.17440 23600.00 22000.00 0.00 0.17631 23400.00 22200.00 0.00 0.17770 23600.00 22200.00 0.00 0.17942 23400.00 22400.00 0.00 0.18088 23600.00 22400.00 0.00 0.18239 23400.00 22600.00 0.00 0.18430 23600.00 22600.00 0.00 0.18551 23400.00 22800.00 0.00 0.18780 23600.00 22800.00 0.00 0.18860 23400.00 23000.00 0.00 0.19089 23600.00 23000.00 0.00 0.19118 23400.00 23200.00 0.00 0.19318 23400.00 23400.00 0.00 0.19411 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 23474.13 23348.70 -39.01 0.19427 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/071.ctf NX, NY, NZ: 360 360 1 MODE : real