CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/074.mrc Output diagnostic file name Micrographs/074.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/074.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2208.000 7902.000 Mean, RMS : 4402.752 227.5339 TITLE 1: emi2mrc: 074.emi Sun Jan 19 15:10:01 2014 TITLE 2: kV 300, Mag 78000x, Def 23960, Spot 4, Int 55.14%, Obj 88.04%, Dif 73.67% TITLE 3: X 262.414, Y -470.755, Z -58.220, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.03682 16200.00 8000.00 0.00 -0.03627 16400.00 8000.00 0.00 -0.03552 16600.00 8000.00 0.00 -0.03471 16800.00 8000.00 0.00 -0.03387 17000.00 8000.00 0.00 -0.03300 17200.00 8000.00 0.00 -0.03208 17400.00 8000.00 0.00 -0.03111 17600.00 8000.00 0.00 -0.03008 17800.00 8000.00 0.00 -0.02900 18000.00 8000.00 0.00 -0.02786 18200.00 8000.00 0.00 -0.02669 18400.00 8000.00 0.00 -0.02552 18600.00 8000.00 0.00 -0.02438 18800.00 8000.00 0.00 -0.02326 19000.00 8000.00 0.00 -0.02210 19200.00 8000.00 0.00 -0.02084 19400.00 8000.00 0.00 -0.01945 19600.00 8000.00 0.00 -0.01795 19800.00 8000.00 0.00 -0.01641 20000.00 8000.00 0.00 -0.01492 20200.00 8000.00 0.00 -0.01351 20400.00 8000.00 0.00 -0.01214 20600.00 8000.00 0.00 -0.01072 20800.00 8000.00 0.00 -0.00917 21000.00 8000.00 0.00 -0.00748 21200.00 8000.00 0.00 -0.00570 21400.00 8000.00 0.00 -0.00390 21600.00 8000.00 0.00 -0.00214 21800.00 8000.00 0.00 -0.00045 22000.00 8000.00 0.00 0.00118 22200.00 8000.00 0.00 0.00281 22400.00 8000.00 0.00 0.00445 22600.00 8000.00 0.00 0.00614 22800.00 8000.00 0.00 0.00788 23000.00 8000.00 0.00 0.00971 23200.00 8000.00 0.00 0.01166 23400.00 8000.00 0.00 0.01373 23600.00 8000.00 0.00 0.01592 23800.00 8000.00 0.00 0.01819 24000.00 8000.00 0.00 0.02054 24200.00 8000.00 0.00 0.02297 24400.00 8000.00 0.00 0.02551 24600.00 8000.00 0.00 0.02818 24800.00 8000.00 0.00 0.03093 25000.00 8000.00 0.00 0.03363 25200.00 8000.00 0.00 0.03610 25400.00 8000.00 0.00 0.03812 25600.00 8000.00 0.00 0.03954 25800.00 8000.00 0.00 0.04037 26000.00 8000.00 0.00 0.04067 25800.00 8200.00 0.00 0.04073 26000.00 8200.00 0.00 0.04102 25800.00 8400.00 0.00 0.04104 26000.00 8400.00 0.00 0.04132 25800.00 8600.00 0.00 0.04132 26000.00 8600.00 0.00 0.04160 26000.00 8800.00 0.00 0.04185 26000.00 9000.00 0.00 0.04207 26000.00 9200.00 0.00 0.04230 25800.00 9400.00 0.00 0.04233 26000.00 9400.00 0.00 0.04255 25800.00 9600.00 0.00 0.04264 26000.00 9600.00 0.00 0.04284 25800.00 9800.00 0.00 0.04301 26000.00 9800.00 0.00 0.04320 25800.00 10000.00 0.00 0.04345 26000.00 10000.00 0.00 0.04364 25800.00 10200.00 0.00 0.04393 26000.00 10200.00 0.00 0.04412 25800.00 10400.00 0.00 0.04444 26000.00 10400.00 0.00 0.04463 25800.00 10600.00 0.00 0.04500 26000.00 10600.00 0.00 0.04519 25800.00 10800.00 0.00 0.04560 26000.00 10800.00 0.00 0.04579 25800.00 11000.00 0.00 0.04628 26000.00 11000.00 0.00 0.04646 25800.00 11200.00 0.00 0.04704 26000.00 11200.00 0.00 0.04720 25800.00 11400.00 0.00 0.04782 26000.00 11400.00 0.00 0.04797 25800.00 11600.00 0.00 0.04858 26000.00 11600.00 0.00 0.04872 25800.00 11800.00 0.00 0.04934 26000.00 11800.00 0.00 0.04946 25800.00 12000.00 0.00 0.05010 26000.00 12000.00 0.00 0.05021 25600.00 12200.00 0.00 0.05024 25800.00 12200.00 0.00 0.05092 26000.00 12200.00 0.00 0.05101 25600.00 12400.00 0.00 0.05118 25800.00 12400.00 0.00 0.05184 26000.00 12400.00 0.00 0.05190 25600.00 12600.00 0.00 0.05217 25800.00 12600.00 0.00 0.05280 26000.00 12600.00 0.00 0.05283 25600.00 12800.00 0.00 0.05314 25800.00 12800.00 0.00 0.05374 25600.00 13000.00 0.00 0.05405 25800.00 13000.00 0.00 0.05461 25600.00 13200.00 0.00 0.05489 25800.00 13200.00 0.00 0.05542 25600.00 13400.00 0.00 0.05569 25800.00 13400.00 0.00 0.05621 25600.00 13600.00 0.00 0.05656 25800.00 13600.00 0.00 0.05706 25600.00 13800.00 0.00 0.05753 25800.00 13800.00 0.00 0.05803 25600.00 14000.00 0.00 0.05857 25800.00 14000.00 0.00 0.05907 25600.00 14200.00 0.00 0.05967 25800.00 14200.00 0.00 0.06017 25600.00 14400.00 0.00 0.06083 25800.00 14400.00 0.00 0.06134 25600.00 14600.00 0.00 0.06204 25800.00 14600.00 0.00 0.06256 25600.00 14800.00 0.00 0.06335 25800.00 14800.00 0.00 0.06387 25600.00 15000.00 0.00 0.06479 25800.00 15000.00 0.00 0.06531 25600.00 15200.00 0.00 0.06630 25800.00 15200.00 0.00 0.06681 25600.00 15400.00 0.00 0.06779 25800.00 15400.00 0.00 0.06830 25600.00 15600.00 0.00 0.06922 25800.00 15600.00 0.00 0.06973 25600.00 15800.00 0.00 0.07061 25800.00 15800.00 0.00 0.07112 25600.00 16000.00 0.00 0.07201 25800.00 16000.00 0.00 0.07252 25600.00 16200.00 0.00 0.07354 25800.00 16200.00 0.00 0.07407 26000.00 16200.00 0.00 0.07407 25600.00 16400.00 0.00 0.07524 25800.00 16400.00 0.00 0.07578 26000.00 16400.00 0.00 0.07580 25600.00 16600.00 0.00 0.07704 25800.00 16600.00 0.00 0.07759 26000.00 16600.00 0.00 0.07761 25600.00 16800.00 0.00 0.07889 25800.00 16800.00 0.00 0.07944 26000.00 16800.00 0.00 0.07945 25400.00 17000.00 0.00 0.07946 25600.00 17000.00 0.00 0.08074 25800.00 17000.00 0.00 0.08128 25400.00 17200.00 0.00 0.08132 25600.00 17200.00 0.00 0.08260 25800.00 17200.00 0.00 0.08313 25400.00 17400.00 0.00 0.08328 25600.00 17400.00 0.00 0.08456 25800.00 17400.00 0.00 0.08509 25400.00 17600.00 0.00 0.08539 25600.00 17600.00 0.00 0.08668 25800.00 17600.00 0.00 0.08721 25400.00 17800.00 0.00 0.08757 25600.00 17800.00 0.00 0.08887 25800.00 17800.00 0.00 0.08941 25400.00 18000.00 0.00 0.08975 25600.00 18000.00 0.00 0.09106 25800.00 18000.00 0.00 0.09159 25400.00 18200.00 0.00 0.09188 25600.00 18200.00 0.00 0.09319 25800.00 18200.00 0.00 0.09371 25400.00 18400.00 0.00 0.09392 25600.00 18400.00 0.00 0.09523 25800.00 18400.00 0.00 0.09573 25400.00 18600.00 0.00 0.09599 25600.00 18600.00 0.00 0.09729 25800.00 18600.00 0.00 0.09777 25400.00 18800.00 0.00 0.09824 25600.00 18800.00 0.00 0.09954 25800.00 18800.00 0.00 0.09999 25400.00 19000.00 0.00 0.10066 25600.00 19000.00 0.00 0.10194 25800.00 19000.00 0.00 0.10235 25400.00 19200.00 0.00 0.10315 25600.00 19200.00 0.00 0.10440 25800.00 19200.00 0.00 0.10476 25400.00 19400.00 0.00 0.10563 25600.00 19400.00 0.00 0.10685 25800.00 19400.00 0.00 0.10716 25400.00 19600.00 0.00 0.10800 25600.00 19600.00 0.00 0.10919 25800.00 19600.00 0.00 0.10946 25400.00 19800.00 0.00 0.11028 25600.00 19800.00 0.00 0.11144 25800.00 19800.00 0.00 0.11168 25400.00 20000.00 0.00 0.11266 25600.00 20000.00 0.00 0.11379 25800.00 20000.00 0.00 0.11398 25400.00 20200.00 0.00 0.11519 25600.00 20200.00 0.00 0.11629 25800.00 20200.00 0.00 0.11642 25400.00 20400.00 0.00 0.11780 25600.00 20400.00 0.00 0.11886 25800.00 20400.00 0.00 0.11893 25400.00 20600.00 0.00 0.12044 25600.00 20600.00 0.00 0.12144 25800.00 20600.00 0.00 0.12145 25400.00 20800.00 0.00 0.12299 25600.00 20800.00 0.00 0.12394 25400.00 21000.00 0.00 0.12536 25600.00 21000.00 0.00 0.12625 25400.00 21200.00 0.00 0.12771 25600.00 21200.00 0.00 0.12853 25400.00 21400.00 0.00 0.13022 25600.00 21400.00 0.00 0.13098 25400.00 21600.00 0.00 0.13288 25600.00 21600.00 0.00 0.13359 25200.00 21800.00 0.00 0.13379 25400.00 21800.00 0.00 0.13567 25600.00 21800.00 0.00 0.13634 25200.00 22000.00 0.00 0.13670 25400.00 22000.00 0.00 0.13857 25600.00 22000.00 0.00 0.13921 25200.00 22200.00 0.00 0.13957 25400.00 22200.00 0.00 0.14144 25600.00 22200.00 0.00 0.14206 25200.00 22400.00 0.00 0.14243 25400.00 22400.00 0.00 0.14430 25600.00 22400.00 0.00 0.14488 25200.00 22600.00 0.00 0.14547 25400.00 22600.00 0.00 0.14732 25600.00 22600.00 0.00 0.14784 25200.00 22800.00 0.00 0.14873 25400.00 22800.00 0.00 0.15055 25600.00 22800.00 0.00 0.15099 25200.00 23000.00 0.00 0.15214 25400.00 23000.00 0.00 0.15390 25600.00 23000.00 0.00 0.15423 25200.00 23200.00 0.00 0.15570 25400.00 23200.00 0.00 0.15737 25600.00 23200.00 0.00 0.15756 25200.00 23400.00 0.00 0.15927 25400.00 23400.00 0.00 0.16084 25600.00 23400.00 0.00 0.16086 25200.00 23600.00 0.00 0.16281 25400.00 23600.00 0.00 0.16424 25200.00 23800.00 0.00 0.16654 25400.00 23800.00 0.00 0.16779 25000.00 24000.00 0.00 0.16798 25200.00 24000.00 0.00 0.17061 25400.00 24000.00 0.00 0.17163 25000.00 24200.00 0.00 0.17251 25200.00 24200.00 0.00 0.17490 25400.00 24200.00 0.00 0.17561 25000.00 24400.00 0.00 0.17723 25200.00 24400.00 0.00 0.17929 25400.00 24400.00 0.00 0.17963 25000.00 24600.00 0.00 0.18178 25200.00 24600.00 0.00 0.18342 25000.00 24800.00 0.00 0.18540 25200.00 24800.00 0.00 0.18655 25000.00 25000.00 0.00 0.18766 25200.00 25000.00 0.00 0.18832 25000.00 25200.00 0.00 0.18847 25200.00 25200.00 0.00 0.18862 25000.00 25200.00 10.00 0.18868 25000.00 25200.00 15.00 0.18878 25000.00 25200.00 20.00 0.18887 25000.00 25200.00 25.00 0.18894 25000.00 25200.00 30.00 0.18899 25000.00 25200.00 35.00 0.18903 25000.00 25200.00 40.00 0.18905 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 25028.05 25283.12 43.00 0.18925 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/074.ctf NX, NY, NZ: 360 360 1 MODE : real