CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/079.mrc Output diagnostic file name Micrographs/079.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/079.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2276.000 7438.000 Mean, RMS : 4371.495 228.8452 TITLE 1: emi2mrc: 079.emi Sun Jan 19 15:16:49 2014 TITLE 2: kV 300, Mag 78000x, Def 23960, Spot 4, Int 55.14%, Obj 88.04%, Dif 73.67% TITLE 3: X 269.512, Y -461.288, Z -58.220, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.03985 16200.00 8000.00 0.00 -0.03962 16400.00 8000.00 0.00 -0.03872 16600.00 8000.00 0.00 -0.03779 16800.00 8000.00 0.00 -0.03685 17000.00 8000.00 0.00 -0.03590 17200.00 8000.00 0.00 -0.03493 17400.00 8000.00 0.00 -0.03393 17600.00 8000.00 0.00 -0.03291 17800.00 8000.00 0.00 -0.03184 18000.00 8000.00 0.00 -0.03066 18200.00 8000.00 0.00 -0.02934 18400.00 8000.00 0.00 -0.02788 18600.00 8000.00 0.00 -0.02630 18800.00 8000.00 0.00 -0.02465 19000.00 8000.00 0.00 -0.02300 19200.00 8000.00 0.00 -0.02138 19400.00 8000.00 0.00 -0.01981 19600.00 8000.00 0.00 -0.01831 19800.00 8000.00 0.00 -0.01685 20000.00 8000.00 0.00 -0.01542 20200.00 8000.00 0.00 -0.01396 20400.00 8000.00 0.00 -0.01246 20600.00 8000.00 0.00 -0.01091 20800.00 8000.00 0.00 -0.00937 21000.00 8000.00 0.00 -0.00786 21200.00 8000.00 0.00 -0.00641 21400.00 8000.00 0.00 -0.00497 21600.00 8000.00 0.00 -0.00343 21800.00 8000.00 0.00 -0.00173 22000.00 8000.00 0.00 0.00015 22200.00 8000.00 0.00 0.00215 22400.00 8000.00 0.00 0.00416 22600.00 8000.00 0.00 0.00613 22800.00 8000.00 0.00 0.00807 23000.00 8000.00 0.00 0.01002 23200.00 8000.00 0.00 0.01208 23400.00 8000.00 0.00 0.01424 23600.00 8000.00 0.00 0.01645 23800.00 8000.00 0.00 0.01868 24000.00 8000.00 0.00 0.02092 24200.00 8000.00 0.00 0.02323 24400.00 8000.00 0.00 0.02570 24600.00 8000.00 0.00 0.02835 24800.00 8000.00 0.00 0.03104 25000.00 8000.00 0.00 0.03353 25200.00 8000.00 0.00 0.03556 25400.00 8000.00 0.00 0.03692 25600.00 8000.00 0.00 0.03758 25800.00 8000.00 0.00 0.03770 25600.00 8200.00 0.00 0.03783 25800.00 8200.00 0.00 0.03795 25600.00 8400.00 0.00 0.03816 25800.00 8400.00 0.00 0.03830 25600.00 8600.00 0.00 0.03861 25800.00 8600.00 0.00 0.03877 25600.00 8800.00 0.00 0.03913 25800.00 8800.00 0.00 0.03931 25600.00 9000.00 0.00 0.03964 25800.00 9000.00 0.00 0.03985 25600.00 9200.00 0.00 0.04009 25800.00 9200.00 0.00 0.04033 25600.00 9400.00 0.00 0.04047 25800.00 9400.00 0.00 0.04075 25600.00 9600.00 0.00 0.04082 25800.00 9600.00 0.00 0.04112 25600.00 9800.00 0.00 0.04122 25800.00 9800.00 0.00 0.04153 26000.00 9800.00 0.00 0.04154 25600.00 10000.00 0.00 0.04174 25800.00 10000.00 0.00 0.04206 25600.00 10200.00 0.00 0.04236 25800.00 10200.00 0.00 0.04267 25600.00 10400.00 0.00 0.04305 25800.00 10400.00 0.00 0.04335 25600.00 10600.00 0.00 0.04376 25800.00 10600.00 0.00 0.04404 25600.00 10800.00 0.00 0.04448 25800.00 10800.00 0.00 0.04476 25600.00 11000.00 0.00 0.04528 25800.00 11000.00 0.00 0.04557 25600.00 11200.00 0.00 0.04624 25800.00 11200.00 0.00 0.04655 25600.00 11400.00 0.00 0.04735 25800.00 11400.00 0.00 0.04767 25600.00 11600.00 0.00 0.04853 25800.00 11600.00 0.00 0.04886 25600.00 11800.00 0.00 0.04970 25800.00 11800.00 0.00 0.05001 25600.00 12000.00 0.00 0.05075 25800.00 12000.00 0.00 0.05103 25600.00 12200.00 0.00 0.05169 25800.00 12200.00 0.00 0.05193 25600.00 12400.00 0.00 0.05259 25800.00 12400.00 0.00 0.05278 25600.00 12600.00 0.00 0.05351 25800.00 12600.00 0.00 0.05365 25400.00 12800.00 0.00 0.05378 25600.00 12800.00 0.00 0.05448 25800.00 12800.00 0.00 0.05458 25400.00 13000.00 0.00 0.05484 25600.00 13000.00 0.00 0.05551 25800.00 13000.00 0.00 0.05558 25400.00 13200.00 0.00 0.05594 25600.00 13200.00 0.00 0.05657 25800.00 13200.00 0.00 0.05662 25400.00 13400.00 0.00 0.05702 25600.00 13400.00 0.00 0.05763 25800.00 13400.00 0.00 0.05766 25400.00 13600.00 0.00 0.05812 25600.00 13600.00 0.00 0.05871 25800.00 13600.00 0.00 0.05873 25400.00 13800.00 0.00 0.05925 25600.00 13800.00 0.00 0.05982 25800.00 13800.00 0.00 0.05982 25400.00 14000.00 0.00 0.06035 25600.00 14000.00 0.00 0.06090 25400.00 14200.00 0.00 0.06142 25600.00 14200.00 0.00 0.06197 25400.00 14400.00 0.00 0.06248 25600.00 14400.00 0.00 0.06304 25800.00 14400.00 0.00 0.06304 25400.00 14600.00 0.00 0.06357 25600.00 14600.00 0.00 0.06414 25800.00 14600.00 0.00 0.06415 25400.00 14800.00 0.00 0.06478 25600.00 14800.00 0.00 0.06536 25800.00 14800.00 0.00 0.06538 25400.00 15000.00 0.00 0.06622 25600.00 15000.00 0.00 0.06681 25800.00 15000.00 0.00 0.06682 25400.00 15200.00 0.00 0.06786 25600.00 15200.00 0.00 0.06844 25400.00 15400.00 0.00 0.06959 25600.00 15400.00 0.00 0.07016 25400.00 15600.00 0.00 0.07133 25600.00 15600.00 0.00 0.07187 25400.00 15800.00 0.00 0.07296 25600.00 15800.00 0.00 0.07346 25400.00 16000.00 0.00 0.07447 25600.00 16000.00 0.00 0.07492 25400.00 16200.00 0.00 0.07598 25600.00 16200.00 0.00 0.07639 25400.00 16400.00 0.00 0.07759 25600.00 16400.00 0.00 0.07795 25200.00 16600.00 0.00 0.07807 25400.00 16600.00 0.00 0.07930 25600.00 16600.00 0.00 0.07964 25200.00 16800.00 0.00 0.07990 25400.00 16800.00 0.00 0.08110 25600.00 16800.00 0.00 0.08142 25200.00 17000.00 0.00 0.08174 25400.00 17000.00 0.00 0.08292 25600.00 17000.00 0.00 0.08323 25200.00 17200.00 0.00 0.08353 25400.00 17200.00 0.00 0.08471 25600.00 17200.00 0.00 0.08502 25200.00 17400.00 0.00 0.08536 25400.00 17400.00 0.00 0.08654 25600.00 17400.00 0.00 0.08686 25200.00 17600.00 0.00 0.08731 25400.00 17600.00 0.00 0.08849 25600.00 17600.00 0.00 0.08882 25200.00 17800.00 0.00 0.08939 25400.00 17800.00 0.00 0.09058 25600.00 17800.00 0.00 0.09092 25200.00 18000.00 0.00 0.09161 25400.00 18000.00 0.00 0.09282 25600.00 18000.00 0.00 0.09314 25200.00 18200.00 0.00 0.09395 25400.00 18200.00 0.00 0.09514 25600.00 18200.00 0.00 0.09544 25200.00 18400.00 0.00 0.09629 25400.00 18400.00 0.00 0.09745 25600.00 18400.00 0.00 0.09772 25200.00 18600.00 0.00 0.09859 25400.00 18600.00 0.00 0.09973 25600.00 18600.00 0.00 0.09996 25200.00 18800.00 0.00 0.10091 25400.00 18800.00 0.00 0.10202 25600.00 18800.00 0.00 0.10221 25200.00 19000.00 0.00 0.10318 25400.00 19000.00 0.00 0.10427 25600.00 19000.00 0.00 0.10441 25200.00 19200.00 0.00 0.10536 25400.00 19200.00 0.00 0.10643 25600.00 19200.00 0.00 0.10653 25200.00 19400.00 0.00 0.10748 25400.00 19400.00 0.00 0.10854 25600.00 19400.00 0.00 0.10861 25200.00 19600.00 0.00 0.10954 25400.00 19600.00 0.00 0.11060 25600.00 19600.00 0.00 0.11066 25200.00 19800.00 0.00 0.11162 25400.00 19800.00 0.00 0.11269 25600.00 19800.00 0.00 0.11276 25200.00 20000.00 0.00 0.11390 25400.00 20000.00 0.00 0.11498 25600.00 20000.00 0.00 0.11503 25200.00 20200.00 0.00 0.11638 25400.00 20200.00 0.00 0.11745 25600.00 20200.00 0.00 0.11746 25200.00 20400.00 0.00 0.11892 25400.00 20400.00 0.00 0.11997 25200.00 20600.00 0.00 0.12145 25400.00 20600.00 0.00 0.12246 25200.00 20800.00 0.00 0.12389 25400.00 20800.00 0.00 0.12485 25200.00 21000.00 0.00 0.12620 25400.00 21000.00 0.00 0.12710 25200.00 21200.00 0.00 0.12855 25400.00 21200.00 0.00 0.12939 25200.00 21400.00 0.00 0.13114 25400.00 21400.00 0.00 0.13191 25200.00 21600.00 0.00 0.13390 25400.00 21600.00 0.00 0.13459 25000.00 21800.00 0.00 0.13473 25200.00 21800.00 0.00 0.13672 25400.00 21800.00 0.00 0.13731 25000.00 22000.00 0.00 0.13761 25200.00 22000.00 0.00 0.13953 25400.00 22000.00 0.00 0.14002 25000.00 22200.00 0.00 0.14037 25200.00 22200.00 0.00 0.14221 25400.00 22200.00 0.00 0.14260 25000.00 22400.00 0.00 0.14310 25200.00 22400.00 0.00 0.14486 25400.00 22400.00 0.00 0.14514 25000.00 22600.00 0.00 0.14607 25200.00 22600.00 0.00 0.14774 25400.00 22600.00 0.00 0.14788 25000.00 22800.00 0.00 0.14932 25200.00 22800.00 0.00 0.15088 25000.00 23000.00 0.00 0.15272 25200.00 23000.00 0.00 0.15416 25000.00 23200.00 0.00 0.15616 25200.00 23200.00 0.00 0.15746 25000.00 23400.00 0.00 0.15943 25200.00 23400.00 0.00 0.16058 25000.00 23600.00 0.00 0.16248 25200.00 23600.00 0.00 0.16348 25000.00 23800.00 0.00 0.16566 25200.00 23800.00 0.00 0.16644 24800.00 24000.00 0.00 0.16675 25000.00 24000.00 0.00 0.16914 25200.00 24000.00 0.00 0.16963 24800.00 24200.00 0.00 0.17065 25000.00 24200.00 0.00 0.17273 25200.00 24200.00 0.00 0.17283 24800.00 24400.00 0.00 0.17453 25000.00 24400.00 0.00 0.17618 24800.00 24600.00 0.00 0.17783 25000.00 24600.00 0.00 0.17891 24800.00 24800.00 0.00 0.17969 25000.00 24800.00 0.00 0.18015 24800.00 25000.00 20.00 0.18016 24800.00 25000.00 25.00 0.18024 24800.00 25000.00 30.00 0.18032 24800.00 25000.00 35.00 0.18039 24800.00 25000.00 40.00 0.18045 24800.00 25000.00 45.00 0.18049 24800.00 25000.00 50.00 0.18052 24800.00 25000.00 55.00 0.18052 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 24818.02 25000.00 55.00 0.18053 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/079.ctf NX, NY, NZ: 360 360 1 MODE : real