CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/082.mrc Output diagnostic file name Micrographs/082.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/082.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2438.000 8334.000 Mean, RMS : 4400.078 224.0391 TITLE 1: emi2mrc: 082.emi Sun Jan 19 15:14:01 2014 TITLE 2: kV 300, Mag 78000x, Def 23960, Spot 4, Int 55.14%, Obj 88.04%, Dif 73.67% TITLE 3: X 265.134, Y -464.522, Z -58.220, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 118 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.03839 14800.00 8000.00 0.00 -0.03800 15000.00 8000.00 0.00 -0.03732 15200.00 8000.00 0.00 -0.03654 15400.00 8000.00 0.00 -0.03567 15600.00 8000.00 0.00 -0.03479 15800.00 8000.00 0.00 -0.03396 16000.00 8000.00 0.00 -0.03319 16200.00 8000.00 0.00 -0.03248 16400.00 8000.00 0.00 -0.03178 16600.00 8000.00 0.00 -0.03103 16800.00 8000.00 0.00 -0.03016 17000.00 8000.00 0.00 -0.02917 17200.00 8000.00 0.00 -0.02806 17400.00 8000.00 0.00 -0.02686 17600.00 8000.00 0.00 -0.02559 17800.00 8000.00 0.00 -0.02426 18000.00 8000.00 0.00 -0.02285 18200.00 8000.00 0.00 -0.02135 18400.00 8000.00 0.00 -0.01978 18600.00 8000.00 0.00 -0.01816 18800.00 8000.00 0.00 -0.01653 19000.00 8000.00 0.00 -0.01489 19200.00 8000.00 0.00 -0.01322 19400.00 8000.00 0.00 -0.01146 19600.00 8000.00 0.00 -0.00959 19800.00 8000.00 0.00 -0.00763 20000.00 8000.00 0.00 -0.00565 20200.00 8000.00 0.00 -0.00373 20400.00 8000.00 0.00 -0.00192 20600.00 8000.00 0.00 -0.00022 20800.00 8000.00 0.00 0.00144 21000.00 8000.00 0.00 0.00312 21200.00 8000.00 0.00 0.00486 21400.00 8000.00 0.00 0.00667 21600.00 8000.00 0.00 0.00852 21800.00 8000.00 0.00 0.01042 22000.00 8000.00 0.00 0.01242 22200.00 8000.00 0.00 0.01457 22400.00 8000.00 0.00 0.01690 22600.00 8000.00 0.00 0.01935 22800.00 8000.00 0.00 0.02183 23000.00 8000.00 0.00 0.02423 23200.00 8000.00 0.00 0.02659 23400.00 8000.00 0.00 0.02907 23600.00 8000.00 0.00 0.03188 23800.00 8000.00 0.00 0.03518 24000.00 8000.00 0.00 0.03889 24200.00 8000.00 0.00 0.04266 24400.00 8000.00 0.00 0.04602 24600.00 8000.00 0.00 0.04858 24800.00 8000.00 0.00 0.05012 25000.00 8000.00 0.00 0.05080 25200.00 8000.00 0.00 0.05093 25000.00 8200.00 0.00 0.05114 25200.00 8200.00 0.00 0.05125 25000.00 8400.00 0.00 0.05159 25200.00 8400.00 0.00 0.05168 25000.00 8600.00 0.00 0.05219 25200.00 8600.00 0.00 0.05226 24800.00 8800.00 0.00 0.05231 25000.00 8800.00 0.00 0.05289 25200.00 8800.00 0.00 0.05294 24800.00 9000.00 0.00 0.05306 25000.00 9000.00 0.00 0.05361 25200.00 9000.00 0.00 0.05364 24800.00 9200.00 0.00 0.05381 25000.00 9200.00 0.00 0.05432 24800.00 9400.00 0.00 0.05451 25000.00 9400.00 0.00 0.05499 24800.00 9600.00 0.00 0.05514 25000.00 9600.00 0.00 0.05559 24800.00 9800.00 0.00 0.05575 25000.00 9800.00 0.00 0.05617 24800.00 10000.00 0.00 0.05634 25000.00 10000.00 0.00 0.05674 24800.00 10200.00 0.00 0.05686 25000.00 10200.00 0.00 0.05725 24800.00 10400.00 0.00 0.05733 25000.00 10400.00 0.00 0.05772 24800.00 10600.00 0.00 0.05779 25000.00 10600.00 0.00 0.05820 24800.00 10800.00 0.00 0.05830 25000.00 10800.00 0.00 0.05875 24800.00 11000.00 0.00 0.05895 25000.00 11000.00 0.00 0.05943 25200.00 11000.00 0.00 0.05944 24800.00 11200.00 0.00 0.05977 25000.00 11200.00 0.00 0.06028 25200.00 11200.00 0.00 0.06032 24800.00 11400.00 0.00 0.06071 25000.00 11400.00 0.00 0.06124 25200.00 11400.00 0.00 0.06128 24800.00 11600.00 0.00 0.06168 25000.00 11600.00 0.00 0.06223 25200.00 11600.00 0.00 0.06225 24800.00 11800.00 0.00 0.06265 25000.00 11800.00 0.00 0.06318 24800.00 12000.00 0.00 0.06355 25000.00 12000.00 0.00 0.06406 24800.00 12200.00 0.00 0.06440 25000.00 12200.00 0.00 0.06488 24800.00 12400.00 0.00 0.06526 25000.00 12400.00 0.00 0.06569 24800.00 12600.00 0.00 0.06612 25000.00 12600.00 0.00 0.06652 24800.00 12800.00 0.00 0.06699 25000.00 12800.00 0.00 0.06735 24800.00 13000.00 0.00 0.06791 25000.00 13000.00 0.00 0.06824 24800.00 13200.00 0.00 0.06892 25000.00 13200.00 0.00 0.06923 24800.00 13400.00 0.00 0.07005 25000.00 13400.00 0.00 0.07036 24800.00 13600.00 0.00 0.07137 25000.00 13600.00 0.00 0.07169 24800.00 13800.00 0.00 0.07288 25000.00 13800.00 0.00 0.07320 24800.00 14000.00 0.00 0.07446 25000.00 14000.00 0.00 0.07478 24800.00 14200.00 0.00 0.07602 25000.00 14200.00 0.00 0.07634 24800.00 14400.00 0.00 0.07753 25000.00 14400.00 0.00 0.07785 24800.00 14600.00 0.00 0.07898 25000.00 14600.00 0.00 0.07930 24800.00 14800.00 0.00 0.08045 25000.00 14800.00 0.00 0.08077 24800.00 15000.00 0.00 0.08200 25000.00 15000.00 0.00 0.08231 24800.00 15200.00 0.00 0.08360 25000.00 15200.00 0.00 0.08389 24800.00 15400.00 0.00 0.08517 25000.00 15400.00 0.00 0.08543 24600.00 15600.00 0.00 0.08543 24800.00 15600.00 0.00 0.08670 25000.00 15600.00 0.00 0.08694 24600.00 15800.00 0.00 0.08695 24800.00 15800.00 0.00 0.08821 25000.00 15800.00 0.00 0.08842 24600.00 16000.00 0.00 0.08851 24800.00 16000.00 0.00 0.08975 25000.00 16000.00 0.00 0.08994 24600.00 16200.00 0.00 0.09022 24800.00 16200.00 0.00 0.09144 25000.00 16200.00 0.00 0.09159 24600.00 16400.00 0.00 0.09204 24800.00 16400.00 0.00 0.09323 25000.00 16400.00 0.00 0.09334 24600.00 16600.00 0.00 0.09388 24800.00 16600.00 0.00 0.09504 25000.00 16600.00 0.00 0.09510 24600.00 16800.00 0.00 0.09571 24800.00 16800.00 0.00 0.09684 25000.00 16800.00 0.00 0.09685 24600.00 17000.00 0.00 0.09751 24800.00 17000.00 0.00 0.09860 24600.00 17200.00 0.00 0.09932 24800.00 17200.00 0.00 0.10038 24600.00 17400.00 0.00 0.10127 24800.00 17400.00 0.00 0.10232 24600.00 17600.00 0.00 0.10343 24800.00 17600.00 0.00 0.10445 24600.00 17800.00 0.00 0.10564 24800.00 17800.00 0.00 0.10665 24600.00 18000.00 0.00 0.10782 24800.00 18000.00 0.00 0.10882 24600.00 18200.00 0.00 0.10989 24800.00 18200.00 0.00 0.11088 24600.00 18400.00 0.00 0.11183 24800.00 18400.00 0.00 0.11279 24600.00 18600.00 0.00 0.11375 24800.00 18600.00 0.00 0.11468 24600.00 18800.00 0.00 0.11585 24800.00 18800.00 0.00 0.11672 24600.00 19000.00 0.00 0.11810 24800.00 19000.00 0.00 0.11892 24600.00 19200.00 0.00 0.12042 24800.00 19200.00 0.00 0.12119 24600.00 19400.00 0.00 0.12277 24800.00 19400.00 0.00 0.12347 24600.00 19600.00 0.00 0.12503 24800.00 19600.00 0.00 0.12565 24600.00 19800.00 0.00 0.12720 24800.00 19800.00 0.00 0.12776 24600.00 20000.00 0.00 0.12951 24800.00 20000.00 0.00 0.12999 24600.00 20200.00 0.00 0.13202 24800.00 20200.00 0.00 0.13243 24400.00 20400.00 0.00 0.13263 24600.00 20400.00 0.00 0.13464 24800.00 20400.00 0.00 0.13499 24400.00 20600.00 0.00 0.13536 24600.00 20600.00 0.00 0.13732 24800.00 20600.00 0.00 0.13760 24400.00 20800.00 0.00 0.13804 24600.00 20800.00 0.00 0.13995 24800.00 20800.00 0.00 0.14015 24400.00 21000.00 0.00 0.14054 24600.00 21000.00 0.00 0.14238 24800.00 21000.00 0.00 0.14247 24400.00 21200.00 0.00 0.14292 24600.00 21200.00 0.00 0.14467 24400.00 21400.00 0.00 0.14531 24600.00 21400.00 0.00 0.14695 24400.00 21600.00 0.00 0.14763 24600.00 21600.00 0.00 0.14917 24400.00 21800.00 0.00 0.14988 24600.00 21800.00 0.00 0.15132 24400.00 22000.00 0.00 0.15210 24600.00 22000.00 0.00 0.15343 24400.00 22200.00 0.00 0.15425 24600.00 22200.00 0.00 0.15546 24400.00 22400.00 0.00 0.15641 24600.00 22400.00 0.00 0.15750 24400.00 22600.00 0.00 0.15876 24600.00 22600.00 0.00 0.15970 24400.00 22800.00 0.00 0.16121 24600.00 22800.00 0.00 0.16199 24400.00 23000.00 0.00 0.16358 24600.00 23000.00 0.00 0.16417 24400.00 23200.00 0.00 0.16582 24600.00 23200.00 0.00 0.16620 24400.00 23400.00 0.00 0.16780 24600.00 23400.00 0.00 0.16794 24400.00 23600.00 0.00 0.16946 24400.00 23800.00 0.00 0.17103 24400.00 24000.00 0.00 0.17249 24400.00 24200.00 0.00 0.17341 24400.00 24400.00 0.00 0.17353 24200.00 24600.00 40.00 0.17357 24200.00 24400.00 45.00 0.17359 24200.00 24400.00 50.00 0.17364 24200.00 24400.00 55.00 0.17367 24200.00 24400.00 60.00 0.17368 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 24262.40 24469.50 48.82 0.17384 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/082.ctf NX, NY, NZ: 360 360 1 MODE : real