CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/084.mrc Output diagnostic file name Micrographs/084.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/084.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 1958.000 8816.000 Mean, RMS : 4394.788 227.7822 TITLE 1: emi2mrc: 084.emi Sun Jan 19 15:22:53 2014 TITLE 2: kV 300, Mag 78000x, Def 23630, Spot 4, Int 55.14%, Obj 88.05%, Dif 73.67% TITLE 3: X 259.339, Y -466.774, Z -58.220, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 119 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.03577 13800.00 8000.00 0.00 -0.03543 14000.00 8000.00 0.00 -0.03478 14200.00 8000.00 0.00 -0.03416 14400.00 8000.00 0.00 -0.03363 14600.00 8000.00 0.00 -0.03317 14800.00 8000.00 0.00 -0.03274 15000.00 8000.00 0.00 -0.03232 15200.00 8000.00 0.00 -0.03188 15400.00 8000.00 0.00 -0.03138 15600.00 8000.00 0.00 -0.03080 15800.00 8000.00 0.00 -0.03014 16000.00 8000.00 0.00 -0.02933 16200.00 8000.00 0.00 -0.02839 16400.00 8000.00 0.00 -0.02730 16600.00 8000.00 0.00 -0.02611 16800.00 8000.00 0.00 -0.02486 17000.00 8000.00 0.00 -0.02364 17200.00 8000.00 0.00 -0.02249 17400.00 8000.00 0.00 -0.02140 17600.00 8000.00 0.00 -0.02034 17800.00 8000.00 0.00 -0.01921 18000.00 8000.00 0.00 -0.01792 18200.00 8000.00 0.00 -0.01644 18400.00 8000.00 0.00 -0.01481 18600.00 8000.00 0.00 -0.01310 18800.00 8000.00 0.00 -0.01139 19000.00 8000.00 0.00 -0.00973 19200.00 8000.00 0.00 -0.00811 19400.00 8000.00 0.00 -0.00652 19600.00 8000.00 0.00 -0.00497 19800.00 8000.00 0.00 -0.00348 20000.00 8000.00 0.00 -0.00206 20200.00 8000.00 0.00 -0.00070 20400.00 8000.00 0.00 0.00062 20600.00 8000.00 0.00 0.00195 20800.00 8000.00 0.00 0.00334 21000.00 8000.00 0.00 0.00481 21200.00 8000.00 0.00 0.00636 21400.00 8000.00 0.00 0.00796 21600.00 8000.00 0.00 0.00964 21800.00 8000.00 0.00 0.01140 22000.00 8000.00 0.00 0.01329 22200.00 8000.00 0.00 0.01529 22400.00 8000.00 0.00 0.01741 22600.00 8000.00 0.00 0.01960 22800.00 8000.00 0.00 0.02184 23000.00 8000.00 0.00 0.02411 23200.00 8000.00 0.00 0.02645 23400.00 8000.00 0.00 0.02890 23600.00 8000.00 0.00 0.03149 23800.00 8000.00 0.00 0.03421 24000.00 8000.00 0.00 0.03696 24200.00 8000.00 0.00 0.03956 24400.00 8000.00 0.00 0.04181 24600.00 8000.00 0.00 0.04353 24800.00 8000.00 0.00 0.04464 25000.00 8000.00 0.00 0.04519 25200.00 8000.00 0.00 0.04536 25200.00 8200.00 0.00 0.04547 25200.00 8400.00 0.00 0.04556 25000.00 8600.00 0.00 0.04557 25200.00 8600.00 0.00 0.04571 25000.00 8800.00 0.00 0.04582 25200.00 8800.00 0.00 0.04598 25000.00 9000.00 0.00 0.04616 25200.00 9000.00 0.00 0.04634 25000.00 9200.00 0.00 0.04663 25200.00 9200.00 0.00 0.04684 25400.00 9200.00 0.00 0.04684 25000.00 9400.00 0.00 0.04722 25200.00 9400.00 0.00 0.04744 25400.00 9400.00 0.00 0.04746 25000.00 9600.00 0.00 0.04788 25200.00 9600.00 0.00 0.04811 25400.00 9600.00 0.00 0.04812 25000.00 9800.00 0.00 0.04862 25200.00 9800.00 0.00 0.04882 25000.00 10000.00 0.00 0.04937 25200.00 10000.00 0.00 0.04954 25000.00 10200.00 0.00 0.05008 25200.00 10200.00 0.00 0.05021 24800.00 10400.00 0.00 0.05022 25000.00 10400.00 0.00 0.05073 25200.00 10400.00 0.00 0.05083 24800.00 10600.00 0.00 0.05088 25000.00 10600.00 0.00 0.05137 25200.00 10600.00 0.00 0.05147 24800.00 10800.00 0.00 0.05158 25000.00 10800.00 0.00 0.05207 25200.00 10800.00 0.00 0.05216 24800.00 11000.00 0.00 0.05238 25000.00 11000.00 0.00 0.05287 25200.00 11000.00 0.00 0.05296 24800.00 11200.00 0.00 0.05333 25000.00 11200.00 0.00 0.05381 25200.00 11200.00 0.00 0.05390 24800.00 11400.00 0.00 0.05433 25000.00 11400.00 0.00 0.05481 25200.00 11400.00 0.00 0.05489 24800.00 11600.00 0.00 0.05533 25000.00 11600.00 0.00 0.05579 25200.00 11600.00 0.00 0.05585 24800.00 11800.00 0.00 0.05628 25000.00 11800.00 0.00 0.05672 25200.00 11800.00 0.00 0.05676 24800.00 12000.00 0.00 0.05717 25000.00 12000.00 0.00 0.05758 25200.00 12000.00 0.00 0.05759 24800.00 12200.00 0.00 0.05801 25000.00 12200.00 0.00 0.05840 24800.00 12400.00 0.00 0.05886 25000.00 12400.00 0.00 0.05923 24800.00 12600.00 0.00 0.05971 25000.00 12600.00 0.00 0.06008 24800.00 12800.00 0.00 0.06054 25000.00 12800.00 0.00 0.06091 24800.00 13000.00 0.00 0.06140 25000.00 13000.00 0.00 0.06178 24800.00 13200.00 0.00 0.06235 25000.00 13200.00 0.00 0.06273 25200.00 13200.00 0.00 0.06274 24800.00 13400.00 0.00 0.06344 25000.00 13400.00 0.00 0.06383 25200.00 13400.00 0.00 0.06383 24800.00 13600.00 0.00 0.06472 25000.00 13600.00 0.00 0.06511 24800.00 13800.00 0.00 0.06614 25000.00 13800.00 0.00 0.06651 24800.00 14000.00 0.00 0.06751 25000.00 14000.00 0.00 0.06784 24800.00 14200.00 0.00 0.06869 25000.00 14200.00 0.00 0.06897 24800.00 14400.00 0.00 0.06966 25000.00 14400.00 0.00 0.06987 24800.00 14600.00 0.00 0.07047 25000.00 14600.00 0.00 0.07062 24800.00 14800.00 0.00 0.07131 25000.00 14800.00 0.00 0.07141 24600.00 15000.00 0.00 0.07161 24800.00 15000.00 0.00 0.07237 25000.00 15000.00 0.00 0.07242 24600.00 15200.00 0.00 0.07293 24800.00 15200.00 0.00 0.07365 25000.00 15200.00 0.00 0.07366 24600.00 15400.00 0.00 0.07441 24800.00 15400.00 0.00 0.07508 24600.00 15600.00 0.00 0.07597 24800.00 15600.00 0.00 0.07662 24600.00 15800.00 0.00 0.07757 24800.00 15800.00 0.00 0.07821 24600.00 16000.00 0.00 0.07923 24800.00 16000.00 0.00 0.07988 25000.00 16000.00 0.00 0.07989 24600.00 16200.00 0.00 0.08105 24800.00 16200.00 0.00 0.08172 25000.00 16200.00 0.00 0.08176 24600.00 16400.00 0.00 0.08303 24800.00 16400.00 0.00 0.08372 25000.00 16400.00 0.00 0.08379 24600.00 16600.00 0.00 0.08508 24800.00 16600.00 0.00 0.08580 25000.00 16600.00 0.00 0.08589 24600.00 16800.00 0.00 0.08716 24800.00 16800.00 0.00 0.08790 25000.00 16800.00 0.00 0.08801 24600.00 17000.00 0.00 0.08922 24800.00 17000.00 0.00 0.08998 25000.00 17000.00 0.00 0.09009 24600.00 17200.00 0.00 0.09126 24800.00 17200.00 0.00 0.09202 25000.00 17200.00 0.00 0.09213 24600.00 17400.00 0.00 0.09340 24800.00 17400.00 0.00 0.09416 25000.00 17400.00 0.00 0.09425 24600.00 17600.00 0.00 0.09572 24800.00 17600.00 0.00 0.09647 25000.00 17600.00 0.00 0.09652 24400.00 17800.00 0.00 0.09652 24600.00 17800.00 0.00 0.09812 24800.00 17800.00 0.00 0.09886 25000.00 17800.00 0.00 0.09888 24400.00 18000.00 0.00 0.09895 24600.00 18000.00 0.00 0.10055 24800.00 18000.00 0.00 0.10127 24400.00 18200.00 0.00 0.10134 24600.00 18200.00 0.00 0.10294 24800.00 18200.00 0.00 0.10364 24600.00 18400.00 0.00 0.10521 24800.00 18400.00 0.00 0.10589 24600.00 18600.00 0.00 0.10740 24800.00 18600.00 0.00 0.10805 24400.00 18800.00 0.00 0.10805 24600.00 18800.00 0.00 0.10962 24800.00 18800.00 0.00 0.11025 24400.00 19000.00 0.00 0.11031 24600.00 19000.00 0.00 0.11188 24800.00 19000.00 0.00 0.11249 24400.00 19200.00 0.00 0.11257 24600.00 19200.00 0.00 0.11414 24800.00 19200.00 0.00 0.11476 24400.00 19400.00 0.00 0.11488 24600.00 19400.00 0.00 0.11647 24800.00 19400.00 0.00 0.11709 24400.00 19600.00 0.00 0.11724 24600.00 19600.00 0.00 0.11884 24800.00 19600.00 0.00 0.11946 24400.00 19800.00 0.00 0.11966 24600.00 19800.00 0.00 0.12128 24800.00 19800.00 0.00 0.12190 24400.00 20000.00 0.00 0.12230 24600.00 20000.00 0.00 0.12393 24800.00 20000.00 0.00 0.12454 24400.00 20200.00 0.00 0.12514 24600.00 20200.00 0.00 0.12677 24800.00 20200.00 0.00 0.12736 24400.00 20400.00 0.00 0.12799 24600.00 20400.00 0.00 0.12963 24800.00 20400.00 0.00 0.13021 24400.00 20600.00 0.00 0.13081 24600.00 20600.00 0.00 0.13245 24800.00 20600.00 0.00 0.13300 24400.00 20800.00 0.00 0.13355 24600.00 20800.00 0.00 0.13519 24800.00 20800.00 0.00 0.13570 24400.00 21000.00 0.00 0.13618 24600.00 21000.00 0.00 0.13780 24800.00 21000.00 0.00 0.13826 24400.00 21200.00 0.00 0.13885 24600.00 21200.00 0.00 0.14044 24800.00 21200.00 0.00 0.14084 24400.00 21400.00 0.00 0.14173 24600.00 21400.00 0.00 0.14329 24800.00 21400.00 0.00 0.14360 24400.00 21600.00 0.00 0.14477 24600.00 21600.00 0.00 0.14628 24800.00 21600.00 0.00 0.14650 24400.00 21800.00 0.00 0.14792 24600.00 21800.00 0.00 0.14938 24800.00 21800.00 0.00 0.14949 24400.00 22000.00 0.00 0.15124 24600.00 22000.00 0.00 0.15262 24400.00 22200.00 0.00 0.15466 24600.00 22200.00 0.00 0.15596 24400.00 22400.00 0.00 0.15823 24600.00 22400.00 0.00 0.15946 24200.00 22600.00 0.00 0.15965 24400.00 22600.00 0.00 0.16214 24600.00 22600.00 0.00 0.16327 24200.00 22800.00 0.00 0.16383 24400.00 22800.00 0.00 0.16627 24600.00 22800.00 0.00 0.16731 24200.00 23000.00 0.00 0.16811 24400.00 23000.00 0.00 0.17051 24600.00 23000.00 0.00 0.17142 24200.00 23200.00 0.00 0.17256 24400.00 23200.00 0.00 0.17488 24600.00 23200.00 0.00 0.17562 24200.00 23400.00 0.00 0.17711 24400.00 23400.00 0.00 0.17930 24600.00 23400.00 0.00 0.17980 24200.00 23600.00 0.00 0.18163 24400.00 23600.00 0.00 0.18361 24600.00 23600.00 0.00 0.18378 24200.00 23800.00 0.00 0.18615 24400.00 23800.00 0.00 0.18780 24200.00 24000.00 0.00 0.19024 24400.00 24000.00 0.00 0.19139 24200.00 24200.00 0.00 0.19291 24400.00 24200.00 0.00 0.19341 24200.00 24400.00 0.00 0.19349 24200.00 24400.00 5.00 0.19360 24200.00 24400.00 10.00 0.19371 24200.00 24400.00 15.00 0.19381 24200.00 24400.00 20.00 0.19390 24200.00 24400.00 25.00 0.19398 24200.00 24400.00 30.00 0.19403 24200.00 24400.00 35.00 0.19408 24200.00 24400.00 40.00 0.19410 24200.00 24400.00 45.00 0.19410 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 24228.92 24409.33 43.31 0.19413 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/084.ctf NX, NY, NZ: 360 360 1 MODE : real