CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/099.mrc Output diagnostic file name Micrographs/099.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/099.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2282.000 8034.000 Mean, RMS : 4363.016 221.2817 TITLE 1: emi2mrc: 099.emi Sun Jan 19 18:16:51 2014 TITLE 2: kV 300, Mag 78000x, Def 28060, Spot 4, Int 55.13%, Obj 88.08%, Dif 73.67% TITLE 3: X 99.213, Y 707.116, Z -34.314, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.00691 8200.00 8000.00 0.00 -0.00689 8400.00 8000.00 0.00 -0.00686 8600.00 8000.00 0.00 -0.00680 8800.00 8000.00 0.00 -0.00666 9000.00 8000.00 0.00 -0.00641 9200.00 8000.00 0.00 -0.00603 9400.00 8000.00 0.00 -0.00549 9600.00 8000.00 0.00 -0.00478 9800.00 8000.00 0.00 -0.00389 10000.00 8000.00 0.00 -0.00288 10200.00 8000.00 0.00 -0.00182 10400.00 8000.00 0.00 -0.00079 10600.00 8000.00 0.00 0.00017 10800.00 8000.00 0.00 0.00105 11000.00 8000.00 0.00 0.00190 11200.00 8000.00 0.00 0.00279 11400.00 8000.00 0.00 0.00377 11600.00 8000.00 0.00 0.00484 11800.00 8000.00 0.00 0.00601 12000.00 8000.00 0.00 0.00729 12200.00 8000.00 0.00 0.00869 12400.00 8000.00 0.00 0.01022 12600.00 8000.00 0.00 0.01190 12800.00 8000.00 0.00 0.01369 13000.00 8000.00 0.00 0.01557 13200.00 8000.00 0.00 0.01751 13400.00 8000.00 0.00 0.01950 13600.00 8000.00 0.00 0.02157 13800.00 8000.00 0.00 0.02376 14000.00 8000.00 0.00 0.02607 14200.00 8000.00 0.00 0.02845 14400.00 8000.00 0.00 0.03090 14600.00 8000.00 0.00 0.03341 14800.00 8000.00 0.00 0.03601 15000.00 8000.00 0.00 0.03877 15200.00 8000.00 0.00 0.04172 15400.00 8000.00 0.00 0.04481 15600.00 8000.00 0.00 0.04796 15800.00 8000.00 0.00 0.05109 16000.00 8000.00 0.00 0.05416 16200.00 8000.00 0.00 0.05720 16400.00 8000.00 0.00 0.06030 16600.00 8000.00 0.00 0.06346 16800.00 8000.00 0.00 0.06671 17000.00 8000.00 0.00 0.07008 17200.00 8000.00 0.00 0.07363 17400.00 8000.00 0.00 0.07745 17600.00 8000.00 0.00 0.08164 17800.00 8000.00 0.00 0.08611 18000.00 8000.00 0.00 0.09053 18200.00 8000.00 0.00 0.09448 18400.00 8000.00 0.00 0.09752 18600.00 8000.00 0.00 0.09943 18800.00 8000.00 0.00 0.10031 19000.00 8000.00 0.00 0.10047 18600.00 8200.00 0.00 0.10053 18800.00 8200.00 0.00 0.10136 19000.00 8200.00 0.00 0.10148 18600.00 8400.00 0.00 0.10166 18800.00 8400.00 0.00 0.10244 19000.00 8400.00 0.00 0.10252 18600.00 8600.00 0.00 0.10298 18800.00 8600.00 0.00 0.10372 19000.00 8600.00 0.00 0.10376 18600.00 8800.00 0.00 0.10443 18800.00 8800.00 0.00 0.10513 19000.00 8800.00 0.00 0.10515 18600.00 9000.00 0.00 0.10586 18800.00 9000.00 0.00 0.10653 19000.00 9000.00 0.00 0.10654 18600.00 9200.00 0.00 0.10720 18800.00 9200.00 0.00 0.10787 19000.00 9200.00 0.00 0.10790 18600.00 9400.00 0.00 0.10849 18800.00 9400.00 0.00 0.10917 19000.00 9400.00 0.00 0.10922 18600.00 9600.00 0.00 0.10981 18800.00 9600.00 0.00 0.11050 19000.00 9600.00 0.00 0.11056 18600.00 9800.00 0.00 0.11134 18800.00 9800.00 0.00 0.11204 19000.00 9800.00 0.00 0.11211 18600.00 10000.00 0.00 0.11311 18800.00 10000.00 0.00 0.11381 19000.00 10000.00 0.00 0.11386 18600.00 10200.00 0.00 0.11493 18800.00 10200.00 0.00 0.11561 19000.00 10200.00 0.00 0.11566 18600.00 10400.00 0.00 0.11666 18800.00 10400.00 0.00 0.11734 19000.00 10400.00 0.00 0.11737 18600.00 10600.00 0.00 0.11828 18800.00 10600.00 0.00 0.11894 19000.00 10600.00 0.00 0.11896 18600.00 10800.00 0.00 0.11982 18800.00 10800.00 0.00 0.12049 19000.00 10800.00 0.00 0.12051 18600.00 11000.00 0.00 0.12155 18800.00 11000.00 0.00 0.12223 19000.00 11000.00 0.00 0.12226 18600.00 11200.00 0.00 0.12367 18800.00 11200.00 0.00 0.12437 19000.00 11200.00 0.00 0.12441 18600.00 11400.00 0.00 0.12611 18800.00 11400.00 0.00 0.12683 19000.00 11400.00 0.00 0.12688 18400.00 11600.00 0.00 0.12695 18600.00 11600.00 0.00 0.12871 18800.00 11600.00 0.00 0.12943 19000.00 11600.00 0.00 0.12947 18400.00 11800.00 0.00 0.12957 18600.00 11800.00 0.00 0.13135 18800.00 11800.00 0.00 0.13206 19000.00 11800.00 0.00 0.13208 18400.00 12000.00 0.00 0.13213 18600.00 12000.00 0.00 0.13390 18800.00 12000.00 0.00 0.13459 18400.00 12200.00 0.00 0.13467 18600.00 12200.00 0.00 0.13643 18800.00 12200.00 0.00 0.13708 18400.00 12400.00 0.00 0.13742 18600.00 12400.00 0.00 0.13916 18800.00 12400.00 0.00 0.13977 18400.00 12600.00 0.00 0.14039 18600.00 12600.00 0.00 0.14212 18800.00 12600.00 0.00 0.14271 18400.00 12800.00 0.00 0.14344 18600.00 12800.00 0.00 0.14517 18800.00 12800.00 0.00 0.14573 18400.00 13000.00 0.00 0.14655 18600.00 13000.00 0.00 0.14827 18800.00 13000.00 0.00 0.14880 18400.00 13200.00 0.00 0.14964 18600.00 13200.00 0.00 0.15135 18800.00 13200.00 0.00 0.15184 18400.00 13400.00 0.00 0.15272 18600.00 13400.00 0.00 0.15441 18800.00 13400.00 0.00 0.15485 18400.00 13600.00 0.00 0.15605 18600.00 13600.00 0.00 0.15771 18800.00 13600.00 0.00 0.15809 18400.00 13800.00 0.00 0.15974 18600.00 13800.00 0.00 0.16136 18800.00 13800.00 0.00 0.16165 18400.00 14000.00 0.00 0.16359 18600.00 14000.00 0.00 0.16514 18800.00 14000.00 0.00 0.16534 18400.00 14200.00 0.00 0.16745 18600.00 14200.00 0.00 0.16893 18800.00 14200.00 0.00 0.16901 18400.00 14400.00 0.00 0.17128 18600.00 14400.00 0.00 0.17267 18400.00 14600.00 0.00 0.17501 18600.00 14600.00 0.00 0.17630 18400.00 14800.00 0.00 0.17882 18600.00 14800.00 0.00 0.18000 18200.00 15000.00 0.00 0.18005 18400.00 15000.00 0.00 0.18296 18600.00 15000.00 0.00 0.18404 18200.00 15200.00 0.00 0.18445 18400.00 15200.00 0.00 0.18731 18600.00 15200.00 0.00 0.18829 18200.00 15400.00 0.00 0.18878 18400.00 15400.00 0.00 0.19160 18600.00 15400.00 0.00 0.19249 18200.00 15600.00 0.00 0.19297 18400.00 15600.00 0.00 0.19577 18600.00 15600.00 0.00 0.19657 18200.00 15800.00 0.00 0.19700 18400.00 15800.00 0.00 0.19978 18600.00 15800.00 0.00 0.20050 18200.00 16000.00 0.00 0.20103 18400.00 16000.00 0.00 0.20379 18600.00 16000.00 0.00 0.20441 18200.00 16200.00 0.00 0.20549 18400.00 16200.00 0.00 0.20820 18600.00 16200.00 0.00 0.20868 18200.00 16400.00 0.00 0.21041 18400.00 16400.00 0.00 0.21304 18600.00 16400.00 0.00 0.21333 18200.00 16600.00 0.00 0.21545 18400.00 16600.00 0.00 0.21794 18600.00 16600.00 0.00 0.21798 18200.00 16800.00 0.00 0.22039 18400.00 16800.00 0.00 0.22271 18200.00 17000.00 0.00 0.22510 18400.00 17000.00 0.00 0.22721 18200.00 17200.00 0.00 0.22966 18400.00 17200.00 0.00 0.23153 18200.00 17400.00 0.00 0.23462 18400.00 17400.00 0.00 0.23615 18000.00 17600.00 0.00 0.23624 18200.00 17600.00 0.00 0.24021 18400.00 17600.00 0.00 0.24125 18000.00 17800.00 0.00 0.24216 18200.00 17800.00 0.00 0.24562 18400.00 17800.00 0.00 0.24597 18000.00 18000.00 0.00 0.24698 18200.00 18000.00 0.00 0.24975 18000.00 18200.00 0.00 0.24982 18200.00 18200.00 0.00 0.25172 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 18197.39 18244.02 26.53 0.25178 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/099.ctf NX, NY, NZ: 360 360 1 MODE : real