CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/108.mrc Output diagnostic file name Micrographs/108.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/108.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2334.000 7874.000 Mean, RMS : 4385.364 221.7194 TITLE 1: emi2mrc: 108.emi Sun Jan 19 18:27:49 2014 TITLE 2: kV 300, Mag 78000x, Def 22150, Spot 4, Int 55.13%, Obj 88.07%, Dif 73.67% TITLE 3: X 94.352, Y 705.636, Z -34.314, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.02602 11000.00 8000.00 0.00 -0.02575 11200.00 8000.00 0.00 -0.02551 11400.00 8000.00 0.00 -0.02533 11600.00 8000.00 0.00 -0.02514 11800.00 8000.00 0.00 -0.02492 12000.00 8000.00 0.00 -0.02463 12200.00 8000.00 0.00 -0.02426 12400.00 8000.00 0.00 -0.02382 12600.00 8000.00 0.00 -0.02330 12800.00 8000.00 0.00 -0.02265 13000.00 8000.00 0.00 -0.02179 13200.00 8000.00 0.00 -0.02070 13400.00 8000.00 0.00 -0.01940 13600.00 8000.00 0.00 -0.01798 13800.00 8000.00 0.00 -0.01653 14000.00 8000.00 0.00 -0.01513 14200.00 8000.00 0.00 -0.01379 14400.00 8000.00 0.00 -0.01248 14600.00 8000.00 0.00 -0.01114 14800.00 8000.00 0.00 -0.00976 15000.00 8000.00 0.00 -0.00831 15200.00 8000.00 0.00 -0.00679 15400.00 8000.00 0.00 -0.00522 15600.00 8000.00 0.00 -0.00358 15800.00 8000.00 0.00 -0.00187 16000.00 8000.00 0.00 -0.00007 16200.00 8000.00 0.00 0.00183 16400.00 8000.00 0.00 0.00385 16600.00 8000.00 0.00 0.00597 16800.00 8000.00 0.00 0.00816 17000.00 8000.00 0.00 0.01039 17200.00 8000.00 0.00 0.01261 17400.00 8000.00 0.00 0.01479 17600.00 8000.00 0.00 0.01695 17800.00 8000.00 0.00 0.01913 18000.00 8000.00 0.00 0.02134 18200.00 8000.00 0.00 0.02360 18400.00 8000.00 0.00 0.02589 18600.00 8000.00 0.00 0.02823 18800.00 8000.00 0.00 0.03064 19000.00 8000.00 0.00 0.03317 19200.00 8000.00 0.00 0.03582 19400.00 8000.00 0.00 0.03858 19600.00 8000.00 0.00 0.04143 19800.00 8000.00 0.00 0.04435 20000.00 8000.00 0.00 0.04738 20200.00 8000.00 0.00 0.05059 20400.00 8000.00 0.00 0.05401 20600.00 8000.00 0.00 0.05758 20800.00 8000.00 0.00 0.06112 21000.00 8000.00 0.00 0.06434 21200.00 8000.00 0.00 0.06698 21400.00 8000.00 0.00 0.06892 21600.00 8000.00 0.00 0.07012 21800.00 8000.00 0.00 0.07070 22000.00 8000.00 0.00 0.07086 21800.00 8200.00 0.00 0.07105 22000.00 8200.00 0.00 0.07123 21800.00 8400.00 0.00 0.07152 22000.00 8400.00 0.00 0.07173 21800.00 8600.00 0.00 0.07229 22000.00 8600.00 0.00 0.07253 21600.00 8800.00 0.00 0.07264 21800.00 8800.00 0.00 0.07331 22000.00 8800.00 0.00 0.07357 22200.00 8800.00 0.00 0.07357 21600.00 9000.00 0.00 0.07377 21800.00 9000.00 0.00 0.07444 22000.00 9000.00 0.00 0.07471 22200.00 9000.00 0.00 0.07472 21600.00 9200.00 0.00 0.07491 21800.00 9200.00 0.00 0.07559 22000.00 9200.00 0.00 0.07586 21600.00 9400.00 0.00 0.07600 21800.00 9400.00 0.00 0.07667 22000.00 9400.00 0.00 0.07692 21600.00 9600.00 0.00 0.07701 21800.00 9600.00 0.00 0.07767 22000.00 9600.00 0.00 0.07789 21600.00 9800.00 0.00 0.07805 21800.00 9800.00 0.00 0.07868 22000.00 9800.00 0.00 0.07887 21600.00 10000.00 0.00 0.07915 21800.00 10000.00 0.00 0.07975 22000.00 10000.00 0.00 0.07991 21600.00 10200.00 0.00 0.08022 21800.00 10200.00 0.00 0.08079 22000.00 10200.00 0.00 0.08092 21600.00 10400.00 0.00 0.08123 21800.00 10400.00 0.00 0.08178 22000.00 10400.00 0.00 0.08189 21600.00 10600.00 0.00 0.08220 21800.00 10600.00 0.00 0.08274 22000.00 10600.00 0.00 0.08284 21600.00 10800.00 0.00 0.08318 21800.00 10800.00 0.00 0.08371 22000.00 10800.00 0.00 0.08381 21600.00 11000.00 0.00 0.08431 21800.00 11000.00 0.00 0.08484 22000.00 11000.00 0.00 0.08494 21600.00 11200.00 0.00 0.08570 21800.00 11200.00 0.00 0.08623 22000.00 11200.00 0.00 0.08635 21600.00 11400.00 0.00 0.08731 21800.00 11400.00 0.00 0.08784 22000.00 11400.00 0.00 0.08797 21600.00 11600.00 0.00 0.08900 21800.00 11600.00 0.00 0.08953 22000.00 11600.00 0.00 0.08966 21600.00 11800.00 0.00 0.09070 21800.00 11800.00 0.00 0.09122 22000.00 11800.00 0.00 0.09135 21600.00 12000.00 0.00 0.09233 21800.00 12000.00 0.00 0.09284 22000.00 12000.00 0.00 0.09296 21600.00 12200.00 0.00 0.09393 21800.00 12200.00 0.00 0.09443 22000.00 12200.00 0.00 0.09454 21600.00 12400.00 0.00 0.09562 21800.00 12400.00 0.00 0.09612 22000.00 12400.00 0.00 0.09623 21400.00 12600.00 0.00 0.09627 21600.00 12600.00 0.00 0.09741 21800.00 12600.00 0.00 0.09792 22000.00 12600.00 0.00 0.09803 21400.00 12800.00 0.00 0.09806 21600.00 12800.00 0.00 0.09922 21800.00 12800.00 0.00 0.09973 22000.00 12800.00 0.00 0.09985 21600.00 13000.00 0.00 0.10099 21800.00 13000.00 0.00 0.10151 22000.00 13000.00 0.00 0.10161 21600.00 13200.00 0.00 0.10268 21800.00 13200.00 0.00 0.10319 22000.00 13200.00 0.00 0.10328 21600.00 13400.00 0.00 0.10430 21800.00 13400.00 0.00 0.10481 22000.00 13400.00 0.00 0.10488 21600.00 13600.00 0.00 0.10603 21800.00 13600.00 0.00 0.10651 22000.00 13600.00 0.00 0.10655 21400.00 13800.00 0.00 0.10674 21600.00 13800.00 0.00 0.10792 21800.00 13800.00 0.00 0.10838 21400.00 14000.00 0.00 0.10874 21600.00 14000.00 0.00 0.10991 21800.00 14000.00 0.00 0.11034 21400.00 14200.00 0.00 0.11080 21600.00 14200.00 0.00 0.11194 21800.00 14200.00 0.00 0.11233 21400.00 14400.00 0.00 0.11289 21600.00 14400.00 0.00 0.11399 21800.00 14400.00 0.00 0.11434 21400.00 14600.00 0.00 0.11499 21600.00 14600.00 0.00 0.11605 21800.00 14600.00 0.00 0.11636 21400.00 14800.00 0.00 0.11722 21600.00 14800.00 0.00 0.11825 21800.00 14800.00 0.00 0.11852 21400.00 15000.00 0.00 0.11972 21600.00 15000.00 0.00 0.12073 21800.00 15000.00 0.00 0.12096 21400.00 15200.00 0.00 0.12240 21600.00 15200.00 0.00 0.12337 21800.00 15200.00 0.00 0.12356 21400.00 15400.00 0.00 0.12508 21600.00 15400.00 0.00 0.12602 21800.00 15400.00 0.00 0.12616 21400.00 15600.00 0.00 0.12768 21600.00 15600.00 0.00 0.12858 21800.00 15600.00 0.00 0.12869 21400.00 15800.00 0.00 0.13014 21600.00 15800.00 0.00 0.13102 21800.00 15800.00 0.00 0.13110 21400.00 16000.00 0.00 0.13256 21600.00 16000.00 0.00 0.13343 21800.00 16000.00 0.00 0.13348 21400.00 16200.00 0.00 0.13521 21600.00 16200.00 0.00 0.13606 21800.00 16200.00 0.00 0.13607 21200.00 16400.00 0.00 0.13625 21400.00 16400.00 0.00 0.13814 21600.00 16400.00 0.00 0.13894 21200.00 16600.00 0.00 0.13930 21400.00 16600.00 0.00 0.14115 21600.00 16600.00 0.00 0.14191 21200.00 16800.00 0.00 0.14234 21400.00 16800.00 0.00 0.14415 21600.00 16800.00 0.00 0.14484 21200.00 17000.00 0.00 0.14527 21400.00 17000.00 0.00 0.14702 21600.00 17000.00 0.00 0.14763 21200.00 17200.00 0.00 0.14800 21400.00 17200.00 0.00 0.14968 21600.00 17200.00 0.00 0.15020 21200.00 17400.00 0.00 0.15075 21400.00 17400.00 0.00 0.15235 21600.00 17400.00 0.00 0.15278 21200.00 17600.00 0.00 0.15371 21400.00 17600.00 0.00 0.15523 21600.00 17600.00 0.00 0.15557 21200.00 17800.00 0.00 0.15676 21400.00 17800.00 0.00 0.15820 21600.00 17800.00 0.00 0.15845 21200.00 18000.00 0.00 0.15978 21400.00 18000.00 0.00 0.16117 21600.00 18000.00 0.00 0.16134 21200.00 18200.00 0.00 0.16274 21400.00 18200.00 0.00 0.16409 21600.00 18200.00 0.00 0.16420 21200.00 18400.00 0.00 0.16559 21400.00 18400.00 0.00 0.16690 21600.00 18400.00 0.00 0.16695 21200.00 18600.00 0.00 0.16849 21400.00 18600.00 0.00 0.16976 21200.00 18800.00 0.00 0.17173 21400.00 18800.00 0.00 0.17292 21200.00 19000.00 0.00 0.17526 21400.00 19000.00 0.00 0.17633 21200.00 19200.00 0.00 0.17884 21400.00 19200.00 0.00 0.17972 21000.00 19400.00 0.00 0.17986 21200.00 19400.00 0.00 0.18229 21400.00 19400.00 0.00 0.18295 21000.00 19600.00 0.00 0.18317 21200.00 19600.00 0.00 0.18544 21400.00 19600.00 0.00 0.18587 21000.00 19800.00 0.00 0.18630 21200.00 19800.00 0.00 0.18839 21400.00 19800.00 0.00 0.18857 21000.00 20000.00 0.00 0.18974 21200.00 20000.00 0.00 0.19160 21000.00 20200.00 0.00 0.19370 21200.00 20200.00 0.00 0.19522 21000.00 20400.00 0.00 0.19784 21200.00 20400.00 0.00 0.19890 21000.00 20600.00 0.00 0.20173 21200.00 20600.00 0.00 0.20220 20800.00 20800.00 0.00 0.20230 21000.00 20800.00 0.00 0.20469 21000.00 21000.00 0.00 0.20587 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 21072.30 20969.69 -42.47 0.20600 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/108.ctf NX, NY, NZ: 360 360 1 MODE : real