CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/116.mrc Output diagnostic file name Micrographs/116.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/116.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2176.000 7966.000 Mean, RMS : 4386.279 222.4258 TITLE 1: emi2mrc: 116.emi Sun Jan 19 18:26:07 2014 TITLE 2: kV 300, Mag 78000x, Def 22150, Spot 4, Int 55.13%, Obj 88.07%, Dif 73.67% TITLE 3: X 91.283, Y 701.685, Z -34.314, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.01794 8200.00 8000.00 0.00 -0.01792 8400.00 8000.00 0.00 -0.01787 9800.00 8000.00 0.00 -0.01761 10000.00 8000.00 0.00 -0.01721 10200.00 8000.00 0.00 -0.01677 10400.00 8000.00 0.00 -0.01628 10600.00 8000.00 0.00 -0.01573 10800.00 8000.00 0.00 -0.01513 11000.00 8000.00 0.00 -0.01447 11200.00 8000.00 0.00 -0.01380 11400.00 8000.00 0.00 -0.01311 11600.00 8000.00 0.00 -0.01237 11800.00 8000.00 0.00 -0.01157 12000.00 8000.00 0.00 -0.01073 12200.00 8000.00 0.00 -0.00988 12400.00 8000.00 0.00 -0.00909 12600.00 8000.00 0.00 -0.00837 12800.00 8000.00 0.00 -0.00770 13000.00 8000.00 0.00 -0.00703 13200.00 8000.00 0.00 -0.00631 13400.00 8000.00 0.00 -0.00551 13600.00 8000.00 0.00 -0.00464 13800.00 8000.00 0.00 -0.00372 14000.00 8000.00 0.00 -0.00275 14200.00 8000.00 0.00 -0.00168 14400.00 8000.00 0.00 -0.00044 14600.00 8000.00 0.00 0.00097 14800.00 8000.00 0.00 0.00255 15000.00 8000.00 0.00 0.00422 15200.00 8000.00 0.00 0.00594 15400.00 8000.00 0.00 0.00769 15600.00 8000.00 0.00 0.00949 15800.00 8000.00 0.00 0.01138 16000.00 8000.00 0.00 0.01340 16200.00 8000.00 0.00 0.01553 16400.00 8000.00 0.00 0.01776 16600.00 8000.00 0.00 0.02005 16800.00 8000.00 0.00 0.02239 17000.00 8000.00 0.00 0.02476 17200.00 8000.00 0.00 0.02716 17400.00 8000.00 0.00 0.02953 17600.00 8000.00 0.00 0.03189 17800.00 8000.00 0.00 0.03424 18000.00 8000.00 0.00 0.03663 18200.00 8000.00 0.00 0.03913 18400.00 8000.00 0.00 0.04182 18600.00 8000.00 0.00 0.04471 18800.00 8000.00 0.00 0.04779 19000.00 8000.00 0.00 0.05102 19200.00 8000.00 0.00 0.05435 19400.00 8000.00 0.00 0.05777 19600.00 8000.00 0.00 0.06127 19800.00 8000.00 0.00 0.06482 20000.00 8000.00 0.00 0.06827 20200.00 8000.00 0.00 0.07141 20400.00 8000.00 0.00 0.07396 20600.00 8000.00 0.00 0.07576 20800.00 8000.00 0.00 0.07679 21000.00 8000.00 0.00 0.07720 21200.00 8000.00 0.00 0.07726 20800.00 8200.00 0.00 0.07764 21000.00 8200.00 0.00 0.07807 21200.00 8200.00 0.00 0.07814 20800.00 8400.00 0.00 0.07851 21000.00 8400.00 0.00 0.07894 21200.00 8400.00 0.00 0.07903 20800.00 8600.00 0.00 0.07949 21000.00 8600.00 0.00 0.07992 21200.00 8600.00 0.00 0.07999 20800.00 8800.00 0.00 0.08056 21000.00 8800.00 0.00 0.08098 21200.00 8800.00 0.00 0.08102 20800.00 9000.00 0.00 0.08162 21000.00 9000.00 0.00 0.08203 21200.00 9000.00 0.00 0.08205 20800.00 9200.00 0.00 0.08267 21000.00 9200.00 0.00 0.08306 20800.00 9400.00 0.00 0.08370 21000.00 9400.00 0.00 0.08408 20800.00 9600.00 0.00 0.08473 21000.00 9600.00 0.00 0.08510 20800.00 9800.00 0.00 0.08594 21000.00 9800.00 0.00 0.08630 20600.00 10000.00 0.00 0.08633 20800.00 10000.00 0.00 0.08739 21000.00 10000.00 0.00 0.08774 20600.00 10200.00 0.00 0.08793 20800.00 10200.00 0.00 0.08898 21000.00 10200.00 0.00 0.08930 20600.00 10400.00 0.00 0.08956 20800.00 10400.00 0.00 0.09059 21000.00 10400.00 0.00 0.09089 20600.00 10600.00 0.00 0.09112 20800.00 10600.00 0.00 0.09214 21000.00 10600.00 0.00 0.09239 20600.00 10800.00 0.00 0.09255 20800.00 10800.00 0.00 0.09354 21000.00 10800.00 0.00 0.09377 20600.00 11000.00 0.00 0.09394 20800.00 11000.00 0.00 0.09491 21000.00 11000.00 0.00 0.09511 20600.00 11200.00 0.00 0.09544 20800.00 11200.00 0.00 0.09640 21000.00 11200.00 0.00 0.09659 20600.00 11400.00 0.00 0.09706 20800.00 11400.00 0.00 0.09801 21000.00 11400.00 0.00 0.09819 20600.00 11600.00 0.00 0.09874 20800.00 11600.00 0.00 0.09969 21000.00 11600.00 0.00 0.09984 20600.00 11800.00 0.00 0.10044 20800.00 11800.00 0.00 0.10136 21000.00 11800.00 0.00 0.10148 20600.00 12000.00 0.00 0.10205 20800.00 12000.00 0.00 0.10294 21000.00 12000.00 0.00 0.10304 20600.00 12200.00 0.00 0.10361 20800.00 12200.00 0.00 0.10448 21000.00 12200.00 0.00 0.10455 20600.00 12400.00 0.00 0.10528 20800.00 12400.00 0.00 0.10613 21000.00 12400.00 0.00 0.10617 20600.00 12600.00 0.00 0.10710 20800.00 12600.00 0.00 0.10793 21000.00 12600.00 0.00 0.10796 20600.00 12800.00 0.00 0.10902 20800.00 12800.00 0.00 0.10984 20600.00 13000.00 0.00 0.11102 20800.00 13000.00 0.00 0.11182 20600.00 13200.00 0.00 0.11300 20800.00 13200.00 0.00 0.11378 20600.00 13400.00 0.00 0.11490 20800.00 13400.00 0.00 0.11565 20600.00 13600.00 0.00 0.11684 20800.00 13600.00 0.00 0.11758 20600.00 13800.00 0.00 0.11893 20800.00 13800.00 0.00 0.11965 20600.00 14000.00 0.00 0.12111 20800.00 14000.00 0.00 0.12182 20600.00 14200.00 0.00 0.12340 20800.00 14200.00 0.00 0.12411 20600.00 14400.00 0.00 0.12582 20800.00 14400.00 0.00 0.12654 20600.00 14600.00 0.00 0.12831 20800.00 14600.00 0.00 0.12905 20400.00 14800.00 0.00 0.12912 20600.00 14800.00 0.00 0.13096 20800.00 14800.00 0.00 0.13169 20400.00 15000.00 0.00 0.13203 20600.00 15000.00 0.00 0.13387 20800.00 15000.00 0.00 0.13458 20400.00 15200.00 0.00 0.13512 20600.00 15200.00 0.00 0.13696 20800.00 15200.00 0.00 0.13764 20400.00 15400.00 0.00 0.13829 20600.00 15400.00 0.00 0.14013 20800.00 15400.00 0.00 0.14077 20400.00 15600.00 0.00 0.14151 20600.00 15600.00 0.00 0.14334 20800.00 15600.00 0.00 0.14395 20400.00 15800.00 0.00 0.14470 20600.00 15800.00 0.00 0.14652 20800.00 15800.00 0.00 0.14709 20400.00 16000.00 0.00 0.14790 20600.00 16000.00 0.00 0.14970 20800.00 16000.00 0.00 0.15021 20400.00 16200.00 0.00 0.15134 20600.00 16200.00 0.00 0.15310 20800.00 16200.00 0.00 0.15356 20400.00 16400.00 0.00 0.15507 20600.00 16400.00 0.00 0.15680 20800.00 16400.00 0.00 0.15718 20400.00 16600.00 0.00 0.15895 20600.00 16600.00 0.00 0.16063 20800.00 16600.00 0.00 0.16094 20400.00 16800.00 0.00 0.16291 20600.00 16800.00 0.00 0.16454 20800.00 16800.00 0.00 0.16477 20400.00 17000.00 0.00 0.16686 20600.00 17000.00 0.00 0.16843 20800.00 17000.00 0.00 0.16859 20400.00 17200.00 0.00 0.17068 20600.00 17200.00 0.00 0.17221 20800.00 17200.00 0.00 0.17227 20400.00 17400.00 0.00 0.17456 20600.00 17400.00 0.00 0.17602 20400.00 17600.00 0.00 0.17869 20600.00 17600.00 0.00 0.18008 20200.00 17800.00 0.00 0.18009 20400.00 17800.00 0.00 0.18301 20600.00 17800.00 0.00 0.18431 20200.00 18000.00 0.00 0.18462 20400.00 18000.00 0.00 0.18747 20600.00 18000.00 0.00 0.18865 20200.00 18200.00 0.00 0.18927 20400.00 18200.00 0.00 0.19205 20600.00 18200.00 0.00 0.19309 20200.00 18400.00 0.00 0.19388 20400.00 18400.00 0.00 0.19657 20600.00 18400.00 0.00 0.19745 20200.00 18600.00 0.00 0.19852 20400.00 18600.00 0.00 0.20108 20600.00 18600.00 0.00 0.20178 20200.00 18800.00 0.00 0.20343 20400.00 18800.00 0.00 0.20583 20600.00 18800.00 0.00 0.20630 20200.00 19000.00 0.00 0.20856 20400.00 19000.00 0.00 0.21077 20600.00 19000.00 0.00 0.21096 20200.00 19200.00 0.00 0.21381 20400.00 19200.00 0.00 0.21576 20200.00 19400.00 0.00 0.21916 20400.00 19400.00 0.00 0.22079 20000.00 19600.00 0.00 0.22113 20200.00 19600.00 0.00 0.22427 20400.00 19600.00 0.00 0.22548 20000.00 19800.00 0.00 0.22588 20200.00 19800.00 0.00 0.22862 20400.00 19800.00 0.00 0.22934 20000.00 20000.00 0.00 0.22981 20200.00 20000.00 0.00 0.23209 20400.00 20000.00 0.00 0.23221 20000.00 20200.00 0.00 0.23253 20200.00 20200.00 0.00 0.23421 20200.00 20400.00 10.00 0.23432 20200.00 20400.00 15.00 0.23441 20200.00 20400.00 20.00 0.23449 20200.00 20400.00 25.00 0.23455 20200.00 20400.00 30.00 0.23459 20200.00 20400.00 35.00 0.23460 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 20176.41 20342.96 35.00 0.23474 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/116.ctf NX, NY, NZ: 360 360 1 MODE : real