CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/120.mrc Output diagnostic file name Micrographs/120.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/120.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2506.000 7864.000 Mean, RMS : 4377.700 222.2549 TITLE 1: emi2mrc: 120.emi Sun Jan 19 18:37:10 2014 TITLE 2: kV 300, Mag 78000x, Def 20840, Spot 4, Int 55.13%, Obj 88.08%, Dif 73.67% TITLE 3: X 85.290, Y 698.665, Z -34.314, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.01162 8400.00 8000.00 0.00 -0.01144 8600.00 8000.00 0.00 -0.01108 8800.00 8000.00 0.00 -0.01065 9000.00 8000.00 0.00 -0.01025 9200.00 8000.00 0.00 -0.00996 9400.00 8000.00 0.00 -0.00978 9600.00 8000.00 0.00 -0.00959 9800.00 8000.00 0.00 -0.00924 10000.00 8000.00 0.00 -0.00866 10200.00 8000.00 0.00 -0.00787 10400.00 8000.00 0.00 -0.00700 10600.00 8000.00 0.00 -0.00619 10800.00 8000.00 0.00 -0.00549 11000.00 8000.00 0.00 -0.00484 11200.00 8000.00 0.00 -0.00410 11400.00 8000.00 0.00 -0.00315 11600.00 8000.00 0.00 -0.00195 11800.00 8000.00 0.00 -0.00057 12000.00 8000.00 0.00 0.00086 12200.00 8000.00 0.00 0.00227 12400.00 8000.00 0.00 0.00366 12600.00 8000.00 0.00 0.00508 12800.00 8000.00 0.00 0.00659 13000.00 8000.00 0.00 0.00818 13200.00 8000.00 0.00 0.00980 13400.00 8000.00 0.00 0.01138 13600.00 8000.00 0.00 0.01295 13800.00 8000.00 0.00 0.01456 14000.00 8000.00 0.00 0.01631 14200.00 8000.00 0.00 0.01823 14400.00 8000.00 0.00 0.02032 14600.00 8000.00 0.00 0.02253 14800.00 8000.00 0.00 0.02487 15000.00 8000.00 0.00 0.02739 15200.00 8000.00 0.00 0.03012 15400.00 8000.00 0.00 0.03302 15600.00 8000.00 0.00 0.03596 15800.00 8000.00 0.00 0.03878 16000.00 8000.00 0.00 0.04144 16200.00 8000.00 0.00 0.04406 16400.00 8000.00 0.00 0.04684 16600.00 8000.00 0.00 0.04994 16800.00 8000.00 0.00 0.05336 17000.00 8000.00 0.00 0.05697 17200.00 8000.00 0.00 0.06059 17400.00 8000.00 0.00 0.06418 17600.00 8000.00 0.00 0.06789 17800.00 8000.00 0.00 0.07188 18000.00 8000.00 0.00 0.07615 18200.00 8000.00 0.00 0.08047 18400.00 8000.00 0.00 0.08437 18600.00 8000.00 0.00 0.08740 18800.00 8000.00 0.00 0.08940 19000.00 8000.00 0.00 0.09053 19200.00 8000.00 0.00 0.09110 19400.00 8000.00 0.00 0.09144 19600.00 8000.00 0.00 0.09166 19800.00 8000.00 0.00 0.09169 19000.00 8200.00 0.00 0.09171 19200.00 8200.00 0.00 0.09231 19400.00 8200.00 0.00 0.09265 19600.00 8200.00 0.00 0.09286 19000.00 8400.00 0.00 0.09299 19200.00 8400.00 0.00 0.09359 19400.00 8400.00 0.00 0.09392 19600.00 8400.00 0.00 0.09408 19000.00 8600.00 0.00 0.09440 19200.00 8600.00 0.00 0.09499 19400.00 8600.00 0.00 0.09527 19600.00 8600.00 0.00 0.09538 19000.00 8800.00 0.00 0.09584 19200.00 8800.00 0.00 0.09639 19400.00 8800.00 0.00 0.09662 19600.00 8800.00 0.00 0.09667 19000.00 9000.00 0.00 0.09715 19200.00 9000.00 0.00 0.09767 19400.00 9000.00 0.00 0.09785 19000.00 9200.00 0.00 0.09836 19200.00 9200.00 0.00 0.09884 19400.00 9200.00 0.00 0.09898 19000.00 9400.00 0.00 0.09953 19200.00 9400.00 0.00 0.09999 19400.00 9400.00 0.00 0.10009 19000.00 9600.00 0.00 0.10081 19200.00 9600.00 0.00 0.10124 19400.00 9600.00 0.00 0.10132 19000.00 9800.00 0.00 0.10241 19200.00 9800.00 0.00 0.10283 19400.00 9800.00 0.00 0.10289 18800.00 10000.00 0.00 0.10324 19000.00 10000.00 0.00 0.10439 19200.00 10000.00 0.00 0.10479 19400.00 10000.00 0.00 0.10484 18800.00 10200.00 0.00 0.10542 19000.00 10200.00 0.00 0.10656 19200.00 10200.00 0.00 0.10695 19400.00 10200.00 0.00 0.10697 18800.00 10400.00 0.00 0.10760 19000.00 10400.00 0.00 0.10873 19200.00 10400.00 0.00 0.10909 18800.00 10600.00 0.00 0.10966 19000.00 10600.00 0.00 0.11077 19200.00 10600.00 0.00 0.11110 18800.00 10800.00 0.00 0.11154 19000.00 10800.00 0.00 0.11263 19200.00 10800.00 0.00 0.11292 18800.00 11000.00 0.00 0.11343 19000.00 11000.00 0.00 0.11448 19200.00 11000.00 0.00 0.11472 18800.00 11200.00 0.00 0.11551 19000.00 11200.00 0.00 0.11652 19200.00 11200.00 0.00 0.11671 18800.00 11400.00 0.00 0.11776 19000.00 11400.00 0.00 0.11871 19200.00 11400.00 0.00 0.11883 18800.00 11600.00 0.00 0.12007 19000.00 11600.00 0.00 0.12096 19200.00 11600.00 0.00 0.12100 18800.00 11800.00 0.00 0.12238 19000.00 11800.00 0.00 0.12320 18800.00 12000.00 0.00 0.12459 19000.00 12000.00 0.00 0.12534 18800.00 12200.00 0.00 0.12680 19000.00 12200.00 0.00 0.12748 18800.00 12400.00 0.00 0.12927 19000.00 12400.00 0.00 0.12990 18600.00 12600.00 0.00 0.13017 18800.00 12600.00 0.00 0.13206 19000.00 12600.00 0.00 0.13263 18600.00 12800.00 0.00 0.13319 18800.00 12800.00 0.00 0.13503 19000.00 12800.00 0.00 0.13553 18600.00 13000.00 0.00 0.13627 18800.00 13000.00 0.00 0.13805 19000.00 13000.00 0.00 0.13848 18600.00 13200.00 0.00 0.13927 18800.00 13200.00 0.00 0.14098 19000.00 13200.00 0.00 0.14133 18600.00 13400.00 0.00 0.14209 18800.00 13400.00 0.00 0.14374 19000.00 13400.00 0.00 0.14402 18600.00 13600.00 0.00 0.14496 18800.00 13600.00 0.00 0.14655 19000.00 13600.00 0.00 0.14677 18600.00 13800.00 0.00 0.14806 18800.00 13800.00 0.00 0.14959 19000.00 13800.00 0.00 0.14974 18600.00 14000.00 0.00 0.15130 18800.00 14000.00 0.00 0.15278 19000.00 14000.00 0.00 0.15286 18600.00 14200.00 0.00 0.15466 18800.00 14200.00 0.00 0.15609 19000.00 14200.00 0.00 0.15609 18600.00 14400.00 0.00 0.15813 18800.00 14400.00 0.00 0.15950 18600.00 14600.00 0.00 0.16163 18800.00 14600.00 0.00 0.16293 18600.00 14800.00 0.00 0.16530 18800.00 14800.00 0.00 0.16653 18600.00 15000.00 0.00 0.16938 18800.00 15000.00 0.00 0.17053 18400.00 15200.00 0.00 0.17074 18600.00 15200.00 0.00 0.17375 18800.00 15200.00 0.00 0.17482 18400.00 15400.00 0.00 0.17521 18600.00 15400.00 0.00 0.17819 18800.00 15400.00 0.00 0.17918 18400.00 15600.00 0.00 0.17966 18600.00 15600.00 0.00 0.18261 18800.00 15600.00 0.00 0.18353 18400.00 15800.00 0.00 0.18397 18600.00 15800.00 0.00 0.18691 18800.00 15800.00 0.00 0.18778 18400.00 16000.00 0.00 0.18829 18600.00 16000.00 0.00 0.19122 18800.00 16000.00 0.00 0.19201 18400.00 16200.00 0.00 0.19301 18600.00 16200.00 0.00 0.19590 18800.00 16200.00 0.00 0.19658 18400.00 16400.00 0.00 0.19823 18600.00 16400.00 0.00 0.20105 18800.00 16400.00 0.00 0.20156 18400.00 16600.00 0.00 0.20366 18600.00 16600.00 0.00 0.20636 18800.00 16600.00 0.00 0.20664 18400.00 16800.00 0.00 0.20906 18600.00 16800.00 0.00 0.21159 18800.00 16800.00 0.00 0.21160 18400.00 17000.00 0.00 0.21424 18600.00 17000.00 0.00 0.21658 18400.00 17200.00 0.00 0.21925 18600.00 17200.00 0.00 0.22138 18400.00 17400.00 0.00 0.22476 18600.00 17400.00 0.00 0.22660 18400.00 17600.00 0.00 0.23132 18600.00 17600.00 0.00 0.23271 18200.00 17800.00 0.00 0.23415 18400.00 17800.00 0.00 0.23854 18600.00 17800.00 0.00 0.23925 18200.00 18000.00 0.00 0.24186 18400.00 18000.00 0.00 0.24556 18200.00 18200.00 0.00 0.24848 18400.00 18200.00 0.00 0.25116 18200.00 18400.00 0.00 0.25236 18400.00 18400.00 0.00 0.25374 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 18342.87 18461.39 13.66 0.25398 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/120.ctf NX, NY, NZ: 360 360 1 MODE : real