CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/121.mrc Output diagnostic file name Micrographs/121.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/121.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2300.000 9126.000 Mean, RMS : 4385.015 224.3161 TITLE 1: emi2mrc: 121.emi Sun Jan 19 18:21:36 2014 TITLE 2: kV 300, Mag 78000x, Def 22150, Spot 4, Int 55.13%, Obj 88.07%, Dif 73.67% TITLE 3: X 91.333, Y 697.436, Z -34.314, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.00970 8200.00 8000.00 0.00 -0.00947 8400.00 8000.00 0.00 -0.00915 8600.00 8000.00 0.00 -0.00883 8800.00 8000.00 0.00 -0.00861 9000.00 8000.00 0.00 -0.00848 9200.00 8000.00 0.00 -0.00842 9400.00 8000.00 0.00 -0.00837 9600.00 8000.00 0.00 -0.00830 9800.00 8000.00 0.00 -0.00818 10000.00 8000.00 0.00 -0.00802 10200.00 8000.00 0.00 -0.00782 10400.00 8000.00 0.00 -0.00752 10600.00 8000.00 0.00 -0.00707 10800.00 8000.00 0.00 -0.00643 11000.00 8000.00 0.00 -0.00560 11200.00 8000.00 0.00 -0.00465 11400.00 8000.00 0.00 -0.00365 11600.00 8000.00 0.00 -0.00264 11800.00 8000.00 0.00 -0.00163 12000.00 8000.00 0.00 -0.00057 12200.00 8000.00 0.00 0.00057 12400.00 8000.00 0.00 0.00182 12600.00 8000.00 0.00 0.00317 12800.00 8000.00 0.00 0.00457 13000.00 8000.00 0.00 0.00598 13200.00 8000.00 0.00 0.00738 13400.00 8000.00 0.00 0.00876 13600.00 8000.00 0.00 0.01018 13800.00 8000.00 0.00 0.01170 14000.00 8000.00 0.00 0.01337 14200.00 8000.00 0.00 0.01519 14400.00 8000.00 0.00 0.01714 14600.00 8000.00 0.00 0.01914 14800.00 8000.00 0.00 0.02115 15000.00 8000.00 0.00 0.02314 15200.00 8000.00 0.00 0.02516 15400.00 8000.00 0.00 0.02725 15600.00 8000.00 0.00 0.02950 15800.00 8000.00 0.00 0.03194 16000.00 8000.00 0.00 0.03453 16200.00 8000.00 0.00 0.03720 16400.00 8000.00 0.00 0.03988 16600.00 8000.00 0.00 0.04251 16800.00 8000.00 0.00 0.04510 17000.00 8000.00 0.00 0.04771 17200.00 8000.00 0.00 0.05040 17400.00 8000.00 0.00 0.05322 17600.00 8000.00 0.00 0.05621 17800.00 8000.00 0.00 0.05936 18000.00 8000.00 0.00 0.06268 18200.00 8000.00 0.00 0.06621 18400.00 8000.00 0.00 0.07000 18600.00 8000.00 0.00 0.07403 18800.00 8000.00 0.00 0.07820 19000.00 8000.00 0.00 0.08223 19200.00 8000.00 0.00 0.08573 19400.00 8000.00 0.00 0.08840 19600.00 8000.00 0.00 0.09009 19800.00 8000.00 0.00 0.09089 20000.00 8000.00 0.00 0.09108 19800.00 8200.00 0.00 0.09170 20000.00 8200.00 0.00 0.09185 19800.00 8400.00 0.00 0.09242 20000.00 8400.00 0.00 0.09254 19800.00 8600.00 0.00 0.09322 20000.00 8600.00 0.00 0.09331 19600.00 8800.00 0.00 0.09340 19800.00 8800.00 0.00 0.09409 20000.00 8800.00 0.00 0.09415 19600.00 9000.00 0.00 0.09426 19800.00 9000.00 0.00 0.09493 20000.00 9000.00 0.00 0.09496 19600.00 9200.00 0.00 0.09506 19800.00 9200.00 0.00 0.09571 20000.00 9200.00 0.00 0.09572 19600.00 9400.00 0.00 0.09581 19800.00 9400.00 0.00 0.09646 20000.00 9400.00 0.00 0.09647 19600.00 9600.00 0.00 0.09661 19800.00 9600.00 0.00 0.09726 20000.00 9600.00 0.00 0.09727 19600.00 9800.00 0.00 0.09765 19800.00 9800.00 0.00 0.09831 20000.00 9800.00 0.00 0.09832 19600.00 10000.00 0.00 0.09900 19800.00 10000.00 0.00 0.09966 20000.00 10000.00 0.00 0.09966 19600.00 10200.00 0.00 0.10052 19800.00 10200.00 0.00 0.10117 19600.00 10400.00 0.00 0.10209 19800.00 10400.00 0.00 0.10272 19600.00 10600.00 0.00 0.10363 19800.00 10600.00 0.00 0.10423 19600.00 10800.00 0.00 0.10513 19800.00 10800.00 0.00 0.10572 19600.00 11000.00 0.00 0.10676 19800.00 11000.00 0.00 0.10733 19600.00 11200.00 0.00 0.10868 19800.00 11200.00 0.00 0.10922 19600.00 11400.00 0.00 0.11081 19800.00 11400.00 0.00 0.11132 19400.00 11600.00 0.00 0.11143 19600.00 11600.00 0.00 0.11300 19800.00 11600.00 0.00 0.11348 19400.00 11800.00 0.00 0.11360 19600.00 11800.00 0.00 0.11515 19800.00 11800.00 0.00 0.11559 19400.00 12000.00 0.00 0.11561 19600.00 12000.00 0.00 0.11713 19800.00 12000.00 0.00 0.11753 19600.00 12200.00 0.00 0.11902 19800.00 12200.00 0.00 0.11939 19400.00 12400.00 0.00 0.11957 19600.00 12400.00 0.00 0.12104 19800.00 12400.00 0.00 0.12137 19400.00 12600.00 0.00 0.12182 19600.00 12600.00 0.00 0.12326 19800.00 12600.00 0.00 0.12355 19400.00 12800.00 0.00 0.12417 19600.00 12800.00 0.00 0.12559 19800.00 12800.00 0.00 0.12585 19400.00 13000.00 0.00 0.12660 19600.00 13000.00 0.00 0.12800 19800.00 13000.00 0.00 0.12824 19400.00 13200.00 0.00 0.12898 19600.00 13200.00 0.00 0.13037 19800.00 13200.00 0.00 0.13058 19400.00 13400.00 0.00 0.13126 19600.00 13400.00 0.00 0.13263 19800.00 13400.00 0.00 0.13282 19400.00 13600.00 0.00 0.13360 19600.00 13600.00 0.00 0.13498 19800.00 13600.00 0.00 0.13514 19400.00 13800.00 0.00 0.13617 19600.00 13800.00 0.00 0.13756 19800.00 13800.00 0.00 0.13771 19400.00 14000.00 0.00 0.13898 19600.00 14000.00 0.00 0.14038 19800.00 14000.00 0.00 0.14054 19400.00 14200.00 0.00 0.14206 19600.00 14200.00 0.00 0.14349 19800.00 14200.00 0.00 0.14364 19400.00 14400.00 0.00 0.14541 19600.00 14400.00 0.00 0.14685 19800.00 14400.00 0.00 0.14697 19400.00 14600.00 0.00 0.14886 19600.00 14600.00 0.00 0.15028 19800.00 14600.00 0.00 0.15036 19400.00 14800.00 0.00 0.15239 19600.00 14800.00 0.00 0.15378 19800.00 14800.00 0.00 0.15379 19400.00 15000.00 0.00 0.15607 19600.00 15000.00 0.00 0.15740 19400.00 15200.00 0.00 0.15974 19600.00 15200.00 0.00 0.16102 19400.00 15400.00 0.00 0.16335 19600.00 15400.00 0.00 0.16458 19400.00 15600.00 0.00 0.16699 19600.00 15600.00 0.00 0.16818 19400.00 15800.00 0.00 0.17071 19600.00 15800.00 0.00 0.17186 19400.00 16000.00 0.00 0.17456 19600.00 16000.00 0.00 0.17567 19200.00 16200.00 0.00 0.17592 19400.00 16200.00 0.00 0.17872 19600.00 16200.00 0.00 0.17977 19200.00 16400.00 0.00 0.18036 19400.00 16400.00 0.00 0.18314 19600.00 16400.00 0.00 0.18411 19200.00 16600.00 0.00 0.18482 19400.00 16600.00 0.00 0.18756 19600.00 16600.00 0.00 0.18844 19200.00 16800.00 0.00 0.18928 19400.00 16800.00 0.00 0.19198 19600.00 16800.00 0.00 0.19275 19200.00 17000.00 0.00 0.19379 19400.00 17000.00 0.00 0.19642 19600.00 17000.00 0.00 0.19707 19200.00 17200.00 0.00 0.19838 19400.00 17200.00 0.00 0.20093 19600.00 17200.00 0.00 0.20143 19200.00 17400.00 0.00 0.20329 19400.00 17400.00 0.00 0.20573 19600.00 17400.00 0.00 0.20606 19200.00 17600.00 0.00 0.20859 19400.00 17600.00 0.00 0.21091 19600.00 17600.00 0.00 0.21103 19200.00 17800.00 0.00 0.21399 19400.00 17800.00 0.00 0.21616 19200.00 18000.00 0.00 0.21939 19400.00 18000.00 0.00 0.22138 19200.00 18200.00 0.00 0.22490 19400.00 18200.00 0.00 0.22665 19200.00 18400.00 0.00 0.23050 19400.00 18400.00 0.00 0.23192 19000.00 18600.00 0.00 0.23265 19200.00 18600.00 0.00 0.23631 19400.00 18600.00 0.00 0.23729 19000.00 18800.00 0.00 0.23910 19200.00 18800.00 0.00 0.24229 19400.00 18800.00 0.00 0.24263 19000.00 19000.00 0.00 0.24491 19200.00 19000.00 0.00 0.24735 19000.00 19200.00 0.00 0.24846 19200.00 19200.00 0.00 0.24995 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 19156.61 19286.24 20.53 0.25024 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/121.ctf NX, NY, NZ: 360 360 1 MODE : real