CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/127.mrc Output diagnostic file name Micrographs/127.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/127.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 1644.000 7836.000 Mean, RMS : 4363.767 228.5196 TITLE 1: emi2mrc: 127.emi Sun Jan 19 18:45:42 2014 TITLE 2: kV 300, Mag 78000x, Def 20180, Spot 4, Int 55.13%, Obj 88.08%, Dif 73.67% TITLE 3: X 101.583, Y 706.724, Z -34.314, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.00495 8200.00 8000.00 0.00 -0.00452 8400.00 8000.00 0.00 -0.00394 8600.00 8000.00 0.00 -0.00330 8800.00 8000.00 0.00 -0.00266 9000.00 8000.00 0.00 -0.00207 9200.00 8000.00 0.00 -0.00150 9400.00 8000.00 0.00 -0.00098 9600.00 8000.00 0.00 -0.00049 9800.00 8000.00 0.00 -0.00006 10000.00 8000.00 0.00 0.00030 10200.00 8000.00 0.00 0.00066 10400.00 8000.00 0.00 0.00108 10600.00 8000.00 0.00 0.00164 10800.00 8000.00 0.00 0.00239 11000.00 8000.00 0.00 0.00333 11200.00 8000.00 0.00 0.00443 11400.00 8000.00 0.00 0.00566 11600.00 8000.00 0.00 0.00704 11800.00 8000.00 0.00 0.00856 12000.00 8000.00 0.00 0.01022 12200.00 8000.00 0.00 0.01195 12400.00 8000.00 0.00 0.01369 12600.00 8000.00 0.00 0.01542 12800.00 8000.00 0.00 0.01713 13000.00 8000.00 0.00 0.01891 13200.00 8000.00 0.00 0.02081 13400.00 8000.00 0.00 0.02287 13600.00 8000.00 0.00 0.02508 13800.00 8000.00 0.00 0.02740 14000.00 8000.00 0.00 0.02982 14200.00 8000.00 0.00 0.03233 14400.00 8000.00 0.00 0.03495 14600.00 8000.00 0.00 0.03769 14800.00 8000.00 0.00 0.04049 15000.00 8000.00 0.00 0.04331 15200.00 8000.00 0.00 0.04612 15400.00 8000.00 0.00 0.04891 15600.00 8000.00 0.00 0.05178 15800.00 8000.00 0.00 0.05482 16000.00 8000.00 0.00 0.05808 16200.00 8000.00 0.00 0.06156 16400.00 8000.00 0.00 0.06521 16600.00 8000.00 0.00 0.06894 16800.00 8000.00 0.00 0.07277 17000.00 8000.00 0.00 0.07678 17200.00 8000.00 0.00 0.08103 17400.00 8000.00 0.00 0.08547 17600.00 8000.00 0.00 0.08993 17800.00 8000.00 0.00 0.09398 18000.00 8000.00 0.00 0.09719 18200.00 8000.00 0.00 0.09929 18400.00 8000.00 0.00 0.10026 18600.00 8000.00 0.00 0.10037 18200.00 8200.00 0.00 0.10064 18400.00 8200.00 0.00 0.10159 18600.00 8200.00 0.00 0.10167 18200.00 8400.00 0.00 0.10193 18400.00 8400.00 0.00 0.10285 18600.00 8400.00 0.00 0.10290 18200.00 8600.00 0.00 0.10333 18400.00 8600.00 0.00 0.10422 18600.00 8600.00 0.00 0.10423 18200.00 8800.00 0.00 0.10485 18400.00 8800.00 0.00 0.10571 18200.00 9000.00 0.00 0.10643 18400.00 9000.00 0.00 0.10724 18200.00 9200.00 0.00 0.10802 18400.00 9200.00 0.00 0.10879 18200.00 9400.00 0.00 0.10958 18400.00 9400.00 0.00 0.11031 18200.00 9600.00 0.00 0.11107 18400.00 9600.00 0.00 0.11177 18200.00 9800.00 0.00 0.11263 18400.00 9800.00 0.00 0.11331 18200.00 10000.00 0.00 0.11432 18400.00 10000.00 0.00 0.11500 18200.00 10200.00 0.00 0.11607 18400.00 10200.00 0.00 0.11676 18200.00 10400.00 0.00 0.11788 18400.00 10400.00 0.00 0.11859 18200.00 10600.00 0.00 0.11976 18400.00 10600.00 0.00 0.12048 18200.00 10800.00 0.00 0.12168 18400.00 10800.00 0.00 0.12240 18200.00 11000.00 0.00 0.12374 18400.00 11000.00 0.00 0.12446 18200.00 11200.00 0.00 0.12608 18400.00 11200.00 0.00 0.12678 18200.00 11400.00 0.00 0.12860 18400.00 11400.00 0.00 0.12928 18200.00 11600.00 0.00 0.13125 18400.00 11600.00 0.00 0.13191 18000.00 11800.00 0.00 0.13207 18200.00 11800.00 0.00 0.13407 18400.00 11800.00 0.00 0.13473 18000.00 12000.00 0.00 0.13504 18200.00 12000.00 0.00 0.13705 18400.00 12000.00 0.00 0.13771 18000.00 12200.00 0.00 0.13820 18200.00 12200.00 0.00 0.14023 18400.00 12200.00 0.00 0.14086 18000.00 12400.00 0.00 0.14170 18200.00 12400.00 0.00 0.14372 18400.00 12400.00 0.00 0.14431 18000.00 12600.00 0.00 0.14542 18200.00 12600.00 0.00 0.14741 18400.00 12600.00 0.00 0.14795 18000.00 12800.00 0.00 0.14914 18200.00 12800.00 0.00 0.15109 18400.00 12800.00 0.00 0.15157 18000.00 13000.00 0.00 0.15279 18200.00 13000.00 0.00 0.15472 18400.00 13000.00 0.00 0.15513 18000.00 13200.00 0.00 0.15635 18200.00 13200.00 0.00 0.15824 18400.00 13200.00 0.00 0.15857 18000.00 13400.00 0.00 0.15981 18200.00 13400.00 0.00 0.16165 18400.00 13400.00 0.00 0.16191 18000.00 13600.00 0.00 0.16342 18200.00 13600.00 0.00 0.16520 18400.00 13600.00 0.00 0.16539 18000.00 13800.00 0.00 0.16729 18200.00 13800.00 0.00 0.16901 18400.00 13800.00 0.00 0.16910 18000.00 14000.00 0.00 0.17125 18200.00 14000.00 0.00 0.17290 18000.00 14200.00 0.00 0.17523 18200.00 14200.00 0.00 0.17680 18000.00 14400.00 0.00 0.17926 18200.00 14400.00 0.00 0.18075 18000.00 14600.00 0.00 0.18332 18200.00 14600.00 0.00 0.18473 18000.00 14800.00 0.00 0.18756 18200.00 14800.00 0.00 0.18887 17800.00 15000.00 0.00 0.18905 18000.00 15000.00 0.00 0.19218 18200.00 15000.00 0.00 0.19340 17800.00 15200.00 0.00 0.19395 18000.00 15200.00 0.00 0.19702 18200.00 15200.00 0.00 0.19812 17800.00 15400.00 0.00 0.19880 18000.00 15400.00 0.00 0.20181 18200.00 15400.00 0.00 0.20279 17800.00 15600.00 0.00 0.20357 18000.00 15600.00 0.00 0.20650 18200.00 15600.00 0.00 0.20735 17800.00 15800.00 0.00 0.20818 18000.00 15800.00 0.00 0.21102 18200.00 15800.00 0.00 0.21173 17800.00 16000.00 0.00 0.21274 18000.00 16000.00 0.00 0.21549 18200.00 16000.00 0.00 0.21602 17800.00 16200.00 0.00 0.21763 18000.00 16200.00 0.00 0.22024 18200.00 16200.00 0.00 0.22056 17800.00 16400.00 0.00 0.22287 18000.00 16400.00 0.00 0.22531 18200.00 16400.00 0.00 0.22538 17800.00 16600.00 0.00 0.22819 18000.00 16600.00 0.00 0.23042 17800.00 16800.00 0.00 0.23357 18000.00 16800.00 0.00 0.23553 17800.00 17000.00 0.00 0.23888 18000.00 17000.00 0.00 0.24049 17800.00 17200.00 0.00 0.24387 18000.00 17200.00 0.00 0.24507 17600.00 17400.00 0.00 0.24521 17800.00 17400.00 0.00 0.24864 18000.00 17400.00 0.00 0.24929 17600.00 17600.00 0.00 0.25006 17800.00 17600.00 0.00 0.25294 17600.00 17800.00 0.00 0.25351 17800.00 17800.00 0.00 0.25560 17800.00 18000.00 5.00 0.25563 17800.00 18000.00 10.00 0.25567 17800.00 18000.00 15.00 0.25570 17800.00 18000.00 20.00 0.25572 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 17790.14 17910.85 20.77 0.25604 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/127.ctf NX, NY, NZ: 360 360 1 MODE : real