CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/129.mrc Output diagnostic file name Micrographs/129.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/129.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 1954.000 8002.000 Mean, RMS : 4303.878 218.9808 TITLE 1: emi2mrc: 129.emi Sun Jan 19 18:49:21 2014 TITLE 2: kV 300, Mag 78000x, Def 20180, Spot 4, Int 55.13%, Obj 88.08%, Dif 73.67% TITLE 3: X 111.460, Y 714.328, Z -34.314, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 119 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.02355 8200.00 8000.00 0.00 -0.02324 8400.00 8000.00 0.00 -0.02276 8600.00 8000.00 0.00 -0.02225 8800.00 8000.00 0.00 -0.02182 9000.00 8000.00 0.00 -0.02145 9200.00 8000.00 0.00 -0.02107 9400.00 8000.00 0.00 -0.02066 9600.00 8000.00 0.00 -0.02019 9800.00 8000.00 0.00 -0.01970 10000.00 8000.00 0.00 -0.01927 10200.00 8000.00 0.00 -0.01889 10400.00 8000.00 0.00 -0.01851 10600.00 8000.00 0.00 -0.01811 10800.00 8000.00 0.00 -0.01764 11000.00 8000.00 0.00 -0.01710 11200.00 8000.00 0.00 -0.01652 11400.00 8000.00 0.00 -0.01591 11600.00 8000.00 0.00 -0.01521 11800.00 8000.00 0.00 -0.01438 12000.00 8000.00 0.00 -0.01342 12200.00 8000.00 0.00 -0.01233 12400.00 8000.00 0.00 -0.01118 12600.00 8000.00 0.00 -0.01001 12800.00 8000.00 0.00 -0.00878 13000.00 8000.00 0.00 -0.00746 13200.00 8000.00 0.00 -0.00599 13400.00 8000.00 0.00 -0.00434 13600.00 8000.00 0.00 -0.00254 13800.00 8000.00 0.00 -0.00064 14000.00 8000.00 0.00 0.00133 14200.00 8000.00 0.00 0.00336 14400.00 8000.00 0.00 0.00544 14600.00 8000.00 0.00 0.00761 14800.00 8000.00 0.00 0.00986 15000.00 8000.00 0.00 0.01219 15200.00 8000.00 0.00 0.01457 15400.00 8000.00 0.00 0.01696 15600.00 8000.00 0.00 0.01932 15800.00 8000.00 0.00 0.02163 16000.00 8000.00 0.00 0.02392 16200.00 8000.00 0.00 0.02628 16400.00 8000.00 0.00 0.02880 16600.00 8000.00 0.00 0.03150 16800.00 8000.00 0.00 0.03436 17000.00 8000.00 0.00 0.03729 17200.00 8000.00 0.00 0.04018 17400.00 8000.00 0.00 0.04299 17600.00 8000.00 0.00 0.04579 17800.00 8000.00 0.00 0.04865 18000.00 8000.00 0.00 0.05161 18200.00 8000.00 0.00 0.05466 18400.00 8000.00 0.00 0.05769 18600.00 8000.00 0.00 0.06066 18800.00 8000.00 0.00 0.06360 19000.00 8000.00 0.00 0.06665 19200.00 8000.00 0.00 0.06988 19400.00 8000.00 0.00 0.07326 19600.00 8000.00 0.00 0.07658 19800.00 8000.00 0.00 0.07948 20000.00 8000.00 0.00 0.08168 20200.00 8000.00 0.00 0.08304 20400.00 8000.00 0.00 0.08362 20600.00 8000.00 0.00 0.08366 20200.00 8200.00 0.00 0.08384 20400.00 8200.00 0.00 0.08443 20600.00 8200.00 0.00 0.08449 20200.00 8400.00 0.00 0.08461 20400.00 8400.00 0.00 0.08521 20600.00 8400.00 0.00 0.08529 20200.00 8600.00 0.00 0.08547 20400.00 8600.00 0.00 0.08608 20600.00 8600.00 0.00 0.08617 20200.00 8800.00 0.00 0.08638 20400.00 8800.00 0.00 0.08700 20600.00 8800.00 0.00 0.08709 20200.00 9000.00 0.00 0.08725 20400.00 9000.00 0.00 0.08788 20600.00 9000.00 0.00 0.08798 20200.00 9200.00 0.00 0.08808 20400.00 9200.00 0.00 0.08872 20600.00 9200.00 0.00 0.08884 20200.00 9400.00 0.00 0.08887 20400.00 9400.00 0.00 0.08953 20600.00 9400.00 0.00 0.08966 20400.00 9600.00 0.00 0.09036 20600.00 9600.00 0.00 0.09050 20200.00 9800.00 0.00 0.09065 20400.00 9800.00 0.00 0.09137 20600.00 9800.00 0.00 0.09153 20200.00 10000.00 0.00 0.09194 20400.00 10000.00 0.00 0.09267 20600.00 10000.00 0.00 0.09283 20200.00 10200.00 0.00 0.09343 20400.00 10200.00 0.00 0.09416 20600.00 10200.00 0.00 0.09431 20200.00 10400.00 0.00 0.09502 20400.00 10400.00 0.00 0.09574 20600.00 10400.00 0.00 0.09588 20200.00 10600.00 0.00 0.09662 20400.00 10600.00 0.00 0.09731 20600.00 10600.00 0.00 0.09742 20200.00 10800.00 0.00 0.09812 20400.00 10800.00 0.00 0.09878 20600.00 10800.00 0.00 0.09885 20200.00 11000.00 0.00 0.09960 20400.00 11000.00 0.00 0.10022 20600.00 11000.00 0.00 0.10027 20200.00 11200.00 0.00 0.10118 20400.00 11200.00 0.00 0.10178 20600.00 11200.00 0.00 0.10182 20200.00 11400.00 0.00 0.10285 20400.00 11400.00 0.00 0.10346 20600.00 11400.00 0.00 0.10351 20200.00 11600.00 0.00 0.10459 20400.00 11600.00 0.00 0.10520 20600.00 11600.00 0.00 0.10527 20200.00 11800.00 0.00 0.10634 20400.00 11800.00 0.00 0.10696 20600.00 11800.00 0.00 0.10705 20200.00 12000.00 0.00 0.10802 20400.00 12000.00 0.00 0.10865 20600.00 12000.00 0.00 0.10874 20200.00 12200.00 0.00 0.10965 20400.00 12200.00 0.00 0.11028 20600.00 12200.00 0.00 0.11038 20200.00 12400.00 0.00 0.11139 20400.00 12400.00 0.00 0.11202 20600.00 12400.00 0.00 0.11211 20200.00 12600.00 0.00 0.11327 20400.00 12600.00 0.00 0.11389 20600.00 12600.00 0.00 0.11396 20200.00 12800.00 0.00 0.11521 20400.00 12800.00 0.00 0.11583 20600.00 12800.00 0.00 0.11589 20200.00 13000.00 0.00 0.11719 20400.00 13000.00 0.00 0.11781 20600.00 13000.00 0.00 0.11785 20200.00 13200.00 0.00 0.11914 20400.00 13200.00 0.00 0.11975 20600.00 13200.00 0.00 0.11979 20200.00 13400.00 0.00 0.12101 20400.00 13400.00 0.00 0.12162 20600.00 13400.00 0.00 0.12166 20200.00 13600.00 0.00 0.12297 20400.00 13600.00 0.00 0.12358 20600.00 13600.00 0.00 0.12362 20000.00 13800.00 0.00 0.12366 20200.00 13800.00 0.00 0.12512 20400.00 13800.00 0.00 0.12573 20600.00 13800.00 0.00 0.12575 20000.00 14000.00 0.00 0.12592 20200.00 14000.00 0.00 0.12738 20400.00 14000.00 0.00 0.12798 20000.00 14200.00 0.00 0.12825 20200.00 14200.00 0.00 0.12971 20400.00 14200.00 0.00 0.13029 20000.00 14400.00 0.00 0.13061 20200.00 14400.00 0.00 0.13204 20400.00 14400.00 0.00 0.13259 20000.00 14600.00 0.00 0.13288 20200.00 14600.00 0.00 0.13430 20400.00 14600.00 0.00 0.13481 20000.00 14800.00 0.00 0.13516 20200.00 14800.00 0.00 0.13655 20400.00 14800.00 0.00 0.13702 20000.00 15000.00 0.00 0.13757 20200.00 15000.00 0.00 0.13892 20400.00 15000.00 0.00 0.13933 20000.00 15200.00 0.00 0.14004 20200.00 15200.00 0.00 0.14133 20400.00 15200.00 0.00 0.14167 20000.00 15400.00 0.00 0.14245 20200.00 15400.00 0.00 0.14368 20400.00 15400.00 0.00 0.14394 20000.00 15600.00 0.00 0.14481 20200.00 15600.00 0.00 0.14598 20400.00 15600.00 0.00 0.14617 20000.00 15800.00 0.00 0.14707 20200.00 15800.00 0.00 0.14817 20400.00 15800.00 0.00 0.14830 20000.00 16000.00 0.00 0.14928 20200.00 16000.00 0.00 0.15033 20400.00 16000.00 0.00 0.15040 20000.00 16200.00 0.00 0.15169 20200.00 16200.00 0.00 0.15270 20400.00 16200.00 0.00 0.15271 20000.00 16400.00 0.00 0.15435 20200.00 16400.00 0.00 0.15531 20000.00 16600.00 0.00 0.15705 20200.00 16600.00 0.00 0.15794 20000.00 16800.00 0.00 0.15969 20200.00 16800.00 0.00 0.16050 20000.00 17000.00 0.00 0.16215 20200.00 17000.00 0.00 0.16290 20000.00 17200.00 0.00 0.16441 20200.00 17200.00 0.00 0.16508 20000.00 17400.00 0.00 0.16672 20200.00 17400.00 0.00 0.16731 19800.00 17600.00 0.00 0.16753 20000.00 17600.00 0.00 0.16930 20200.00 17600.00 0.00 0.16980 19800.00 17800.00 0.00 0.17037 20000.00 17800.00 0.00 0.17207 20200.00 17800.00 0.00 0.17246 19800.00 18000.00 0.00 0.17328 20000.00 18000.00 0.00 0.17488 20200.00 18000.00 0.00 0.17516 19800.00 18200.00 0.00 0.17612 20000.00 18200.00 0.00 0.17764 20200.00 18200.00 0.00 0.17779 19800.00 18400.00 0.00 0.17875 20000.00 18400.00 0.00 0.18018 20200.00 18400.00 0.00 0.18023 19800.00 18600.00 0.00 0.18136 20000.00 18600.00 0.00 0.18269 19800.00 18800.00 0.00 0.18437 20000.00 18800.00 0.00 0.18553 19800.00 19000.00 0.00 0.18774 20000.00 19000.00 0.00 0.18865 19600.00 19200.00 0.00 0.18874 19800.00 19200.00 0.00 0.19118 20000.00 19200.00 0.00 0.19175 19600.00 19400.00 0.00 0.19237 19800.00 19400.00 0.00 0.19442 20000.00 19400.00 0.00 0.19453 19600.00 19600.00 0.00 0.19532 19800.00 19600.00 0.00 0.19683 19600.00 19800.00 0.00 0.19693 19800.00 19800.00 0.00 0.19789 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 19768.72 19909.88 4.38 0.19801 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/129.ctf NX, NY, NZ: 360 360 1 MODE : real