CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/130.mrc Output diagnostic file name Micrographs/130.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/130.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2044.000 8002.000 Mean, RMS : 4375.383 224.2063 TITLE 1: emi2mrc: 130.emi Sun Jan 19 18:50:04 2014 TITLE 2: kV 300, Mag 78000x, Def 20180, Spot 4, Int 55.13%, Obj 88.08%, Dif 73.67% TITLE 3: X 109.769, Y 712.114, Z -34.314, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 119 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.01065 8200.00 8000.00 0.00 -0.01055 8400.00 8000.00 0.00 -0.01028 8600.00 8000.00 0.00 -0.00991 8800.00 8000.00 0.00 -0.00957 9000.00 8000.00 0.00 -0.00931 9200.00 8000.00 0.00 -0.00913 9400.00 8000.00 0.00 -0.00898 9600.00 8000.00 0.00 -0.00877 9800.00 8000.00 0.00 -0.00848 10000.00 8000.00 0.00 -0.00812 10200.00 8000.00 0.00 -0.00778 10400.00 8000.00 0.00 -0.00747 10600.00 8000.00 0.00 -0.00720 10800.00 8000.00 0.00 -0.00692 11000.00 8000.00 0.00 -0.00655 11200.00 8000.00 0.00 -0.00605 11400.00 8000.00 0.00 -0.00541 11600.00 8000.00 0.00 -0.00466 11800.00 8000.00 0.00 -0.00382 12000.00 8000.00 0.00 -0.00292 12200.00 8000.00 0.00 -0.00197 12400.00 8000.00 0.00 -0.00100 12600.00 8000.00 0.00 -0.00003 12800.00 8000.00 0.00 0.00094 13000.00 8000.00 0.00 0.00196 13200.00 8000.00 0.00 0.00310 13400.00 8000.00 0.00 0.00445 13600.00 8000.00 0.00 0.00603 13800.00 8000.00 0.00 0.00783 14000.00 8000.00 0.00 0.00976 14200.00 8000.00 0.00 0.01174 14400.00 8000.00 0.00 0.01371 14600.00 8000.00 0.00 0.01570 14800.00 8000.00 0.00 0.01772 15000.00 8000.00 0.00 0.01984 15200.00 8000.00 0.00 0.02208 15400.00 8000.00 0.00 0.02441 15600.00 8000.00 0.00 0.02682 15800.00 8000.00 0.00 0.02930 16000.00 8000.00 0.00 0.03183 16200.00 8000.00 0.00 0.03442 16400.00 8000.00 0.00 0.03705 16600.00 8000.00 0.00 0.03968 16800.00 8000.00 0.00 0.04225 17000.00 8000.00 0.00 0.04479 17200.00 8000.00 0.00 0.04734 17400.00 8000.00 0.00 0.04996 17600.00 8000.00 0.00 0.05274 17800.00 8000.00 0.00 0.05567 18000.00 8000.00 0.00 0.05876 18200.00 8000.00 0.00 0.06205 18400.00 8000.00 0.00 0.06562 18600.00 8000.00 0.00 0.06953 18800.00 8000.00 0.00 0.07377 19000.00 8000.00 0.00 0.07811 19200.00 8000.00 0.00 0.08213 19400.00 8000.00 0.00 0.08541 19600.00 8000.00 0.00 0.08768 19800.00 8000.00 0.00 0.08893 20000.00 8000.00 0.00 0.08946 20200.00 8000.00 0.00 0.08963 20400.00 8000.00 0.00 0.08966 19800.00 8200.00 0.00 0.08983 20000.00 8200.00 0.00 0.09037 20200.00 8200.00 0.00 0.09055 20400.00 8200.00 0.00 0.09060 19800.00 8400.00 0.00 0.09070 20000.00 8400.00 0.00 0.09125 20200.00 8400.00 0.00 0.09144 20400.00 8400.00 0.00 0.09150 19800.00 8600.00 0.00 0.09168 20000.00 8600.00 0.00 0.09223 20200.00 8600.00 0.00 0.09243 20400.00 8600.00 0.00 0.09250 19800.00 8800.00 0.00 0.09275 20000.00 8800.00 0.00 0.09331 20200.00 8800.00 0.00 0.09352 20400.00 8800.00 0.00 0.09357 19800.00 9000.00 0.00 0.09381 20000.00 9000.00 0.00 0.09439 20200.00 9000.00 0.00 0.09459 20400.00 9000.00 0.00 0.09464 19800.00 9200.00 0.00 0.09486 20000.00 9200.00 0.00 0.09545 20200.00 9200.00 0.00 0.09565 20400.00 9200.00 0.00 0.09568 19800.00 9400.00 0.00 0.09590 20000.00 9400.00 0.00 0.09649 20200.00 9400.00 0.00 0.09670 20400.00 9400.00 0.00 0.09671 19800.00 9600.00 0.00 0.09696 20000.00 9600.00 0.00 0.09756 20200.00 9600.00 0.00 0.09776 19800.00 9800.00 0.00 0.09819 20000.00 9800.00 0.00 0.09879 20200.00 9800.00 0.00 0.09897 19800.00 10000.00 0.00 0.09963 20000.00 10000.00 0.00 0.10023 20200.00 10000.00 0.00 0.10037 19800.00 10200.00 0.00 0.10118 20000.00 10200.00 0.00 0.10175 20200.00 10200.00 0.00 0.10186 19800.00 10400.00 0.00 0.10274 20000.00 10400.00 0.00 0.10329 20200.00 10400.00 0.00 0.10337 19800.00 10600.00 0.00 0.10433 20000.00 10600.00 0.00 0.10486 20200.00 10600.00 0.00 0.10491 19800.00 10800.00 0.00 0.10596 20000.00 10800.00 0.00 0.10647 20200.00 10800.00 0.00 0.10649 19800.00 11000.00 0.00 0.10777 20000.00 11000.00 0.00 0.10826 19600.00 11200.00 0.00 0.10850 19800.00 11200.00 0.00 0.10987 20000.00 11200.00 0.00 0.11032 19600.00 11400.00 0.00 0.11080 19800.00 11400.00 0.00 0.11215 20000.00 11400.00 0.00 0.11256 19600.00 11600.00 0.00 0.11317 19800.00 11600.00 0.00 0.11450 20000.00 11600.00 0.00 0.11487 19600.00 11800.00 0.00 0.11555 19800.00 11800.00 0.00 0.11685 20000.00 11800.00 0.00 0.11718 19600.00 12000.00 0.00 0.11787 19800.00 12000.00 0.00 0.11915 20000.00 12000.00 0.00 0.11943 19600.00 12200.00 0.00 0.12021 19800.00 12200.00 0.00 0.12145 20000.00 12200.00 0.00 0.12168 19600.00 12400.00 0.00 0.12272 19800.00 12400.00 0.00 0.12391 20000.00 12400.00 0.00 0.12406 19600.00 12600.00 0.00 0.12536 19800.00 12600.00 0.00 0.12648 20000.00 12600.00 0.00 0.12656 19600.00 12800.00 0.00 0.12798 19800.00 12800.00 0.00 0.12904 20000.00 12800.00 0.00 0.12905 19600.00 13000.00 0.00 0.13056 19800.00 13000.00 0.00 0.13155 19600.00 13200.00 0.00 0.13307 19800.00 13200.00 0.00 0.13399 19600.00 13400.00 0.00 0.13550 19800.00 13400.00 0.00 0.13635 19600.00 13600.00 0.00 0.13804 19800.00 13600.00 0.00 0.13883 19600.00 13800.00 0.00 0.14079 19800.00 13800.00 0.00 0.14152 19600.00 14000.00 0.00 0.14361 19800.00 14000.00 0.00 0.14432 19600.00 14200.00 0.00 0.14649 19800.00 14200.00 0.00 0.14718 19400.00 14400.00 0.00 0.14725 19600.00 14400.00 0.00 0.14944 19800.00 14400.00 0.00 0.15012 19400.00 14600.00 0.00 0.15025 19600.00 14600.00 0.00 0.15244 19800.00 14600.00 0.00 0.15311 19400.00 14800.00 0.00 0.15348 19600.00 14800.00 0.00 0.15567 19800.00 14800.00 0.00 0.15631 19400.00 15000.00 0.00 0.15711 19600.00 15000.00 0.00 0.15930 19800.00 15000.00 0.00 0.15990 19400.00 15200.00 0.00 0.16097 19600.00 15200.00 0.00 0.16314 19800.00 15200.00 0.00 0.16368 19400.00 15400.00 0.00 0.16483 19600.00 15400.00 0.00 0.16697 19800.00 15400.00 0.00 0.16743 19400.00 15600.00 0.00 0.16861 19600.00 15600.00 0.00 0.17071 19800.00 15600.00 0.00 0.17108 19400.00 15800.00 0.00 0.17223 19600.00 15800.00 0.00 0.17428 19800.00 15800.00 0.00 0.17454 19400.00 16000.00 0.00 0.17576 19600.00 16000.00 0.00 0.17774 19800.00 16000.00 0.00 0.17788 19400.00 16200.00 0.00 0.17948 19600.00 16200.00 0.00 0.18138 19800.00 16200.00 0.00 0.18139 19400.00 16400.00 0.00 0.18344 19600.00 16400.00 0.00 0.18525 19400.00 16600.00 0.00 0.18743 19600.00 16600.00 0.00 0.18915 19400.00 16800.00 0.00 0.19142 19600.00 16800.00 0.00 0.19304 19400.00 17000.00 0.00 0.19543 19600.00 17000.00 0.00 0.19695 19400.00 17200.00 0.00 0.19953 19600.00 17200.00 0.00 0.20093 19400.00 17400.00 0.00 0.20400 19600.00 17400.00 0.00 0.20527 19200.00 17600.00 0.00 0.20559 19400.00 17600.00 0.00 0.20900 19600.00 17600.00 0.00 0.21010 19200.00 17800.00 0.00 0.21092 19400.00 17800.00 0.00 0.21421 19600.00 17800.00 0.00 0.21509 19200.00 18000.00 0.00 0.21622 19400.00 18000.00 0.00 0.21935 19600.00 18000.00 0.00 0.21995 19200.00 18200.00 0.00 0.22139 19400.00 18200.00 0.00 0.22430 19600.00 18200.00 0.00 0.22454 19200.00 18400.00 0.00 0.22627 19400.00 18400.00 0.00 0.22889 19200.00 18600.00 0.00 0.23104 19400.00 18600.00 0.00 0.23329 19200.00 18800.00 0.00 0.23599 19400.00 18800.00 0.00 0.23773 19200.00 19000.00 0.00 0.24051 19400.00 19000.00 0.00 0.24155 19200.00 19200.00 0.00 0.24350 19400.00 19200.00 0.00 0.24372 19200.00 19400.00 0.00 0.24434 19200.00 19400.00 5.00 0.24443 19200.00 19400.00 10.00 0.24451 19200.00 19400.00 15.00 0.24458 19200.00 19400.00 20.00 0.24463 19200.00 19400.00 25.00 0.24467 19200.00 19400.00 30.00 0.24468 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 19233.45 19383.06 30.00 0.24473 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/130.ctf NX, NY, NZ: 360 360 1 MODE : real