CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/133.mrc Output diagnostic file name Micrographs/133.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/133.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2350.000 8222.000 Mean, RMS : 4376.169 219.7986 TITLE 1: emi2mrc: 133.emi Sun Jan 19 18:52:48 2014 TITLE 2: kV 300, Mag 78000x, Def 20180, Spot 4, Int 55.13%, Obj 88.08%, Dif 73.67% TITLE 3: X 104.788, Y 705.953, Z -34.314, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.00318 8200.00 8000.00 0.00 -0.00289 8400.00 8000.00 0.00 -0.00258 8600.00 8000.00 0.00 -0.00222 8800.00 8000.00 0.00 -0.00181 9000.00 8000.00 0.00 -0.00137 9200.00 8000.00 0.00 -0.00090 9400.00 8000.00 0.00 -0.00038 9600.00 8000.00 0.00 0.00025 9800.00 8000.00 0.00 0.00106 10000.00 8000.00 0.00 0.00207 10200.00 8000.00 0.00 0.00324 10400.00 8000.00 0.00 0.00452 10600.00 8000.00 0.00 0.00587 10800.00 8000.00 0.00 0.00730 11000.00 8000.00 0.00 0.00883 11200.00 8000.00 0.00 0.01049 11400.00 8000.00 0.00 0.01226 11600.00 8000.00 0.00 0.01405 11800.00 8000.00 0.00 0.01577 12000.00 8000.00 0.00 0.01739 12200.00 8000.00 0.00 0.01897 12400.00 8000.00 0.00 0.02064 12600.00 8000.00 0.00 0.02250 12800.00 8000.00 0.00 0.02459 13000.00 8000.00 0.00 0.02685 13200.00 8000.00 0.00 0.02920 13400.00 8000.00 0.00 0.03160 13600.00 8000.00 0.00 0.03410 13800.00 8000.00 0.00 0.03678 14000.00 8000.00 0.00 0.03968 14200.00 8000.00 0.00 0.04272 14400.00 8000.00 0.00 0.04581 14600.00 8000.00 0.00 0.04888 14800.00 8000.00 0.00 0.05192 15000.00 8000.00 0.00 0.05509 15200.00 8000.00 0.00 0.05852 15400.00 8000.00 0.00 0.06221 15600.00 8000.00 0.00 0.06609 15800.00 8000.00 0.00 0.07001 16000.00 8000.00 0.00 0.07385 16200.00 8000.00 0.00 0.07767 16400.00 8000.00 0.00 0.08160 16600.00 8000.00 0.00 0.08567 16800.00 8000.00 0.00 0.08977 17000.00 8000.00 0.00 0.09366 17200.00 8000.00 0.00 0.09700 17400.00 8000.00 0.00 0.09960 17600.00 8000.00 0.00 0.10153 17800.00 8000.00 0.00 0.10294 18000.00 8000.00 0.00 0.10401 18200.00 8000.00 0.00 0.10488 18400.00 8000.00 0.00 0.10554 18600.00 8000.00 0.00 0.10593 18800.00 8000.00 0.00 0.10606 18200.00 8200.00 0.00 0.10664 18400.00 8200.00 0.00 0.10728 18600.00 8200.00 0.00 0.10763 18800.00 8200.00 0.00 0.10771 18200.00 8400.00 0.00 0.10836 18400.00 8400.00 0.00 0.10896 18600.00 8400.00 0.00 0.10926 18800.00 8400.00 0.00 0.10928 18000.00 8600.00 0.00 0.10938 18200.00 8600.00 0.00 0.11020 18400.00 8600.00 0.00 0.11076 18600.00 8600.00 0.00 0.11100 18000.00 8800.00 0.00 0.11134 18200.00 8800.00 0.00 0.11213 18400.00 8800.00 0.00 0.11263 18600.00 8800.00 0.00 0.11280 18000.00 9000.00 0.00 0.11327 18200.00 9000.00 0.00 0.11401 18400.00 9000.00 0.00 0.11444 18600.00 9000.00 0.00 0.11451 18000.00 9200.00 0.00 0.11511 18200.00 9200.00 0.00 0.11578 18400.00 9200.00 0.00 0.11613 18000.00 9400.00 0.00 0.11682 18200.00 9400.00 0.00 0.11742 18400.00 9400.00 0.00 0.11767 18000.00 9600.00 0.00 0.11847 18200.00 9600.00 0.00 0.11898 18400.00 9600.00 0.00 0.11916 17800.00 9800.00 0.00 0.11946 18000.00 9800.00 0.00 0.12025 18200.00 9800.00 0.00 0.12069 18400.00 9800.00 0.00 0.12078 17800.00 10000.00 0.00 0.12153 18000.00 10000.00 0.00 0.12225 18200.00 10000.00 0.00 0.12261 18400.00 10000.00 0.00 0.12262 17800.00 10200.00 0.00 0.12365 18000.00 10200.00 0.00 0.12430 18200.00 10200.00 0.00 0.12458 17600.00 10400.00 0.00 0.12462 17800.00 10400.00 0.00 0.12575 18000.00 10400.00 0.00 0.12633 18200.00 10400.00 0.00 0.12655 17600.00 10600.00 0.00 0.12674 17800.00 10600.00 0.00 0.12781 18000.00 10600.00 0.00 0.12833 18200.00 10600.00 0.00 0.12849 17600.00 10800.00 0.00 0.12883 17800.00 10800.00 0.00 0.12984 18000.00 10800.00 0.00 0.13031 18200.00 10800.00 0.00 0.13044 17600.00 11000.00 0.00 0.13109 17800.00 11000.00 0.00 0.13206 18000.00 11000.00 0.00 0.13249 18200.00 11000.00 0.00 0.13259 17600.00 11200.00 0.00 0.13372 17800.00 11200.00 0.00 0.13466 18000.00 11200.00 0.00 0.13506 18200.00 11200.00 0.00 0.13512 17600.00 11400.00 0.00 0.13659 17800.00 11400.00 0.00 0.13749 18000.00 11400.00 0.00 0.13785 18200.00 11400.00 0.00 0.13787 17600.00 11600.00 0.00 0.13952 17800.00 11600.00 0.00 0.14038 18000.00 11600.00 0.00 0.14069 17400.00 11800.00 0.00 0.14081 17600.00 11800.00 0.00 0.14243 17800.00 11800.00 0.00 0.14325 18000.00 11800.00 0.00 0.14353 17400.00 12000.00 0.00 0.14369 17600.00 12000.00 0.00 0.14529 17800.00 12000.00 0.00 0.14607 18000.00 12000.00 0.00 0.14631 17400.00 12200.00 0.00 0.14667 17600.00 12200.00 0.00 0.14824 17800.00 12200.00 0.00 0.14898 18000.00 12200.00 0.00 0.14919 17400.00 12400.00 0.00 0.15005 17600.00 12400.00 0.00 0.15158 17800.00 12400.00 0.00 0.15228 18000.00 12400.00 0.00 0.15243 17400.00 12600.00 0.00 0.15380 17600.00 12600.00 0.00 0.15528 17800.00 12600.00 0.00 0.15592 18000.00 12600.00 0.00 0.15600 17400.00 12800.00 0.00 0.15768 17600.00 12800.00 0.00 0.15910 17800.00 12800.00 0.00 0.15966 18000.00 12800.00 0.00 0.15968 17400.00 13000.00 0.00 0.16157 17600.00 13000.00 0.00 0.16291 17800.00 13000.00 0.00 0.16340 17400.00 13200.00 0.00 0.16539 17600.00 13200.00 0.00 0.16665 17800.00 13200.00 0.00 0.16705 17400.00 13400.00 0.00 0.16914 17600.00 13400.00 0.00 0.17031 17800.00 13400.00 0.00 0.17063 17200.00 13600.00 0.00 0.17082 17400.00 13600.00 0.00 0.17314 17600.00 13600.00 0.00 0.17422 17800.00 13600.00 0.00 0.17445 17200.00 13800.00 0.00 0.17532 17400.00 13800.00 0.00 0.17755 17600.00 13800.00 0.00 0.17853 17800.00 13800.00 0.00 0.17867 17200.00 14000.00 0.00 0.17999 17400.00 14000.00 0.00 0.18213 17600.00 14000.00 0.00 0.18301 17800.00 14000.00 0.00 0.18306 17200.00 14200.00 0.00 0.18461 17400.00 14200.00 0.00 0.18667 17600.00 14200.00 0.00 0.18746 17200.00 14400.00 0.00 0.18911 17400.00 14400.00 0.00 0.19110 17600.00 14400.00 0.00 0.19182 17200.00 14600.00 0.00 0.19350 17400.00 14600.00 0.00 0.19544 17600.00 14600.00 0.00 0.19609 17200.00 14800.00 0.00 0.19813 17400.00 14800.00 0.00 0.20002 17600.00 14800.00 0.00 0.20060 17200.00 15000.00 0.00 0.20340 17400.00 15000.00 0.00 0.20522 17600.00 15000.00 0.00 0.20569 17200.00 15200.00 0.00 0.20916 17400.00 15200.00 0.00 0.21087 17600.00 15200.00 0.00 0.21118 17000.00 15400.00 0.00 0.21152 17200.00 15400.00 0.00 0.21497 17400.00 15400.00 0.00 0.21653 17600.00 15400.00 0.00 0.21665 17000.00 15600.00 0.00 0.21720 17200.00 15600.00 0.00 0.22058 17400.00 15600.00 0.00 0.22196 17000.00 15800.00 0.00 0.22253 17200.00 15800.00 0.00 0.22583 17400.00 15800.00 0.00 0.22701 17000.00 16000.00 0.00 0.22783 17200.00 16000.00 0.00 0.23101 17400.00 16000.00 0.00 0.23194 17000.00 16200.00 0.00 0.23390 17200.00 16200.00 0.00 0.23686 17400.00 16200.00 0.00 0.23740 17000.00 16400.00 0.00 0.24086 17200.00 16400.00 0.00 0.24339 17000.00 16600.00 0.00 0.24780 17200.00 16600.00 0.00 0.24966 16800.00 16800.00 0.00 0.24976 17000.00 16800.00 0.00 0.25359 17200.00 16800.00 0.00 0.25455 17000.00 17000.00 0.00 0.25696 17000.00 17200.00 15.00 0.25697 17000.00 17200.00 20.00 0.25705 17000.00 17200.00 25.00 0.25712 17000.00 17200.00 30.00 0.25718 17000.00 17200.00 35.00 0.25723 17000.00 17200.00 40.00 0.25726 17000.00 17200.00 45.00 0.25728 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 17000.00 17200.00 47.28 0.25728 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/133.ctf NX, NY, NZ: 360 360 1 MODE : real