CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/137.mrc Output diagnostic file name Micrographs/137.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/137.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2436.000 7830.000 Mean, RMS : 4378.156 221.3297 TITLE 1: emi2mrc: 137.emi Sun Jan 19 18:56:20 2014 TITLE 2: kV 300, Mag 78000x, Def 20180, Spot 4, Int 55.13%, Obj 88.08%, Dif 73.67% TITLE 3: X 98.001, Y 697.838, Z -34.314, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 119 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.00858 8200.00 8000.00 0.00 -0.00845 8400.00 8000.00 0.00 -0.00815 8600.00 8000.00 0.00 -0.00770 8800.00 8000.00 0.00 -0.00719 9000.00 8000.00 0.00 -0.00666 9200.00 8000.00 0.00 -0.00615 9400.00 8000.00 0.00 -0.00564 9600.00 8000.00 0.00 -0.00507 9800.00 8000.00 0.00 -0.00438 10000.00 8000.00 0.00 -0.00358 10200.00 8000.00 0.00 -0.00275 10400.00 8000.00 0.00 -0.00198 10600.00 8000.00 0.00 -0.00130 10800.00 8000.00 0.00 -0.00071 11000.00 8000.00 0.00 -0.00011 11200.00 8000.00 0.00 0.00060 11400.00 8000.00 0.00 0.00147 11600.00 8000.00 0.00 0.00250 11800.00 8000.00 0.00 0.00364 12000.00 8000.00 0.00 0.00485 12200.00 8000.00 0.00 0.00610 12400.00 8000.00 0.00 0.00742 12600.00 8000.00 0.00 0.00886 12800.00 8000.00 0.00 0.01043 13000.00 8000.00 0.00 0.01213 13200.00 8000.00 0.00 0.01394 13400.00 8000.00 0.00 0.01584 13600.00 8000.00 0.00 0.01786 13800.00 8000.00 0.00 0.02004 14000.00 8000.00 0.00 0.02239 14200.00 8000.00 0.00 0.02490 14400.00 8000.00 0.00 0.02755 14600.00 8000.00 0.00 0.03031 14800.00 8000.00 0.00 0.03317 15000.00 8000.00 0.00 0.03614 15200.00 8000.00 0.00 0.03922 15400.00 8000.00 0.00 0.04239 15600.00 8000.00 0.00 0.04558 15800.00 8000.00 0.00 0.04874 16000.00 8000.00 0.00 0.05181 16200.00 8000.00 0.00 0.05482 16400.00 8000.00 0.00 0.05782 16600.00 8000.00 0.00 0.06084 16800.00 8000.00 0.00 0.06393 17000.00 8000.00 0.00 0.06716 17200.00 8000.00 0.00 0.07055 17400.00 8000.00 0.00 0.07415 17600.00 8000.00 0.00 0.07794 17800.00 8000.00 0.00 0.08175 18000.00 8000.00 0.00 0.08529 18200.00 8000.00 0.00 0.08830 18400.00 8000.00 0.00 0.09059 18600.00 8000.00 0.00 0.09214 18800.00 8000.00 0.00 0.09316 19000.00 8000.00 0.00 0.09388 19200.00 8000.00 0.00 0.09441 19400.00 8000.00 0.00 0.09476 19600.00 8000.00 0.00 0.09488 19000.00 8200.00 0.00 0.09497 19200.00 8200.00 0.00 0.09545 19400.00 8200.00 0.00 0.09577 19600.00 8200.00 0.00 0.09584 19000.00 8400.00 0.00 0.09611 19200.00 8400.00 0.00 0.09654 19400.00 8400.00 0.00 0.09681 19600.00 8400.00 0.00 0.09685 19000.00 8600.00 0.00 0.09742 19200.00 8600.00 0.00 0.09780 19400.00 8600.00 0.00 0.09803 19600.00 8600.00 0.00 0.09804 18800.00 8800.00 0.00 0.09831 19000.00 8800.00 0.00 0.09885 19200.00 8800.00 0.00 0.09917 19400.00 8800.00 0.00 0.09936 18800.00 9000.00 0.00 0.09976 19000.00 9000.00 0.00 0.10024 19200.00 9000.00 0.00 0.10052 19400.00 9000.00 0.00 0.10067 18800.00 9200.00 0.00 0.10113 19000.00 9200.00 0.00 0.10156 19200.00 9200.00 0.00 0.10179 19400.00 9200.00 0.00 0.10192 18800.00 9400.00 0.00 0.10243 19000.00 9400.00 0.00 0.10281 19200.00 9400.00 0.00 0.10302 19400.00 9400.00 0.00 0.10312 18800.00 9600.00 0.00 0.10372 19000.00 9600.00 0.00 0.10408 19200.00 9600.00 0.00 0.10427 19400.00 9600.00 0.00 0.10436 18600.00 9800.00 0.00 0.10456 18800.00 9800.00 0.00 0.10524 19000.00 9800.00 0.00 0.10557 19200.00 9800.00 0.00 0.10574 19400.00 9800.00 0.00 0.10583 18600.00 10000.00 0.00 0.10638 18800.00 10000.00 0.00 0.10704 19000.00 10000.00 0.00 0.10735 19200.00 10000.00 0.00 0.10752 19400.00 10000.00 0.00 0.10761 18600.00 10200.00 0.00 0.10838 18800.00 10200.00 0.00 0.10902 19000.00 10200.00 0.00 0.10932 19200.00 10200.00 0.00 0.10947 19400.00 10200.00 0.00 0.10956 18600.00 10400.00 0.00 0.11046 18800.00 10400.00 0.00 0.11108 19000.00 10400.00 0.00 0.11135 19200.00 10400.00 0.00 0.11148 19400.00 10400.00 0.00 0.11155 18600.00 10600.00 0.00 0.11256 18800.00 10600.00 0.00 0.11314 19000.00 10600.00 0.00 0.11337 19200.00 10600.00 0.00 0.11347 19400.00 10600.00 0.00 0.11350 18600.00 10800.00 0.00 0.11465 18800.00 10800.00 0.00 0.11518 19000.00 10800.00 0.00 0.11537 19200.00 10800.00 0.00 0.11542 19400.00 10800.00 0.00 0.11542 18400.00 11000.00 0.00 0.11568 18600.00 11000.00 0.00 0.11686 18800.00 11000.00 0.00 0.11734 19000.00 11000.00 0.00 0.11748 19200.00 11000.00 0.00 0.11749 18400.00 11200.00 0.00 0.11820 18600.00 11200.00 0.00 0.11933 18800.00 11200.00 0.00 0.11975 19000.00 11200.00 0.00 0.11984 18400.00 11400.00 0.00 0.12085 18600.00 11400.00 0.00 0.12194 18800.00 11400.00 0.00 0.12231 19000.00 11400.00 0.00 0.12236 18400.00 11600.00 0.00 0.12349 18600.00 11600.00 0.00 0.12454 18800.00 11600.00 0.00 0.12487 19000.00 11600.00 0.00 0.12489 18400.00 11800.00 0.00 0.12605 18600.00 11800.00 0.00 0.12706 18800.00 11800.00 0.00 0.12735 18400.00 12000.00 0.00 0.12845 18600.00 12000.00 0.00 0.12941 18800.00 12000.00 0.00 0.12966 18400.00 12200.00 0.00 0.13076 18600.00 12200.00 0.00 0.13168 18800.00 12200.00 0.00 0.13189 18400.00 12400.00 0.00 0.13326 18600.00 12400.00 0.00 0.13414 18800.00 12400.00 0.00 0.13431 18400.00 12600.00 0.00 0.13600 18600.00 12600.00 0.00 0.13685 18800.00 12600.00 0.00 0.13700 18400.00 12800.00 0.00 0.13889 18600.00 12800.00 0.00 0.13972 18800.00 12800.00 0.00 0.13984 18200.00 13000.00 0.00 0.13999 18400.00 13000.00 0.00 0.14192 18600.00 13000.00 0.00 0.14272 18800.00 13000.00 0.00 0.14281 18200.00 13200.00 0.00 0.14310 18400.00 13200.00 0.00 0.14501 18600.00 13200.00 0.00 0.14579 18800.00 13200.00 0.00 0.14583 18200.00 13400.00 0.00 0.14623 18400.00 13400.00 0.00 0.14813 18600.00 13400.00 0.00 0.14886 18200.00 13600.00 0.00 0.14959 18400.00 13600.00 0.00 0.15145 18600.00 13600.00 0.00 0.15213 18200.00 13800.00 0.00 0.15326 18400.00 13800.00 0.00 0.15508 18600.00 13800.00 0.00 0.15569 18200.00 14000.00 0.00 0.15705 18400.00 14000.00 0.00 0.15881 18600.00 14000.00 0.00 0.15934 18200.00 14200.00 0.00 0.16086 18400.00 14200.00 0.00 0.16257 18600.00 14200.00 0.00 0.16300 18200.00 14400.00 0.00 0.16469 18400.00 14400.00 0.00 0.16633 18600.00 14400.00 0.00 0.16667 18200.00 14600.00 0.00 0.16849 18400.00 14600.00 0.00 0.17007 18600.00 14600.00 0.00 0.17031 18200.00 14800.00 0.00 0.17242 18400.00 14800.00 0.00 0.17393 18600.00 14800.00 0.00 0.17408 18200.00 15000.00 0.00 0.17671 18400.00 15000.00 0.00 0.17815 18600.00 15000.00 0.00 0.17822 18000.00 15200.00 0.00 0.17837 18200.00 15200.00 0.00 0.18123 18400.00 15200.00 0.00 0.18262 18000.00 15400.00 0.00 0.18293 18200.00 15400.00 0.00 0.18577 18400.00 15400.00 0.00 0.18711 18000.00 15600.00 0.00 0.18748 18200.00 15600.00 0.00 0.19031 18400.00 15600.00 0.00 0.19160 18000.00 15800.00 0.00 0.19198 18200.00 15800.00 0.00 0.19480 18400.00 15800.00 0.00 0.19602 18000.00 16000.00 0.00 0.19654 18200.00 16000.00 0.00 0.19933 18400.00 16000.00 0.00 0.20047 18000.00 16200.00 0.00 0.20146 18200.00 16200.00 0.00 0.20420 18400.00 16200.00 0.00 0.20524 18000.00 16400.00 0.00 0.20670 18200.00 16400.00 0.00 0.20937 18400.00 16400.00 0.00 0.21026 18000.00 16600.00 0.00 0.21192 18200.00 16600.00 0.00 0.21451 18400.00 16600.00 0.00 0.21521 18000.00 16800.00 0.00 0.21703 18200.00 16800.00 0.00 0.21949 18400.00 16800.00 0.00 0.21996 18000.00 17000.00 0.00 0.22200 18200.00 17000.00 0.00 0.22430 18400.00 17000.00 0.00 0.22449 18000.00 17200.00 0.00 0.22686 18200.00 17200.00 0.00 0.22896 18000.00 17400.00 0.00 0.23204 18200.00 17400.00 0.00 0.23383 17800.00 17600.00 0.00 0.23404 18000.00 17600.00 0.00 0.23752 18200.00 17600.00 0.00 0.23882 17800.00 17800.00 0.00 0.23931 18000.00 17800.00 0.00 0.24223 18200.00 17800.00 0.00 0.24285 18000.00 18000.00 0.00 0.24496 18000.00 18200.00 0.00 0.24505 18000.00 18200.00 5.00 0.24507 18000.00 18200.00 10.00 0.24509 18000.00 18200.00 15.00 0.24510 18000.00 18200.00 20.00 0.24512 18000.00 18200.00 25.00 0.24512 18000.00 18200.00 30.00 0.24513 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 18033.87 18103.17 30.00 0.24549 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/137.ctf NX, NY, NZ: 360 360 1 MODE : real