CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/160.mrc Output diagnostic file name Micrographs/160.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/160.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2380.000 7952.000 Mean, RMS : 4479.778 217.5158 TITLE 1: emi2mrc: 160.emi Sun Jan 19 20:39:09 2014 TITLE 2: kV 300, Mag 78000x, Def 17060, Spot 4, Int 55.11%, Obj 88.08%, Dif 73.67% TITLE 3: X 97.425, Y 688.524, Z -34.314, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 0.05375 8200.00 8000.00 0.00 0.05747 8400.00 8000.00 0.00 0.06125 8600.00 8000.00 0.00 0.06524 8800.00 8000.00 0.00 0.06971 9000.00 8000.00 0.00 0.07473 9200.00 8000.00 0.00 0.08036 9400.00 8000.00 0.00 0.08670 9600.00 8000.00 0.00 0.09358 9800.00 8000.00 0.00 0.10078 10000.00 8000.00 0.00 0.10832 10200.00 8000.00 0.00 0.11610 10400.00 8000.00 0.00 0.12403 10600.00 8000.00 0.00 0.13224 10800.00 8000.00 0.00 0.14075 11000.00 8000.00 0.00 0.14936 11200.00 8000.00 0.00 0.15779 11400.00 8000.00 0.00 0.16545 11600.00 8000.00 0.00 0.17147 11800.00 8000.00 0.00 0.17524 12000.00 8000.00 0.00 0.17670 11600.00 8200.00 0.00 0.17694 11800.00 8200.00 0.00 0.18068 12000.00 8200.00 0.00 0.18205 11600.00 8400.00 0.00 0.18258 11800.00 8400.00 0.00 0.18627 12000.00 8400.00 0.00 0.18752 11600.00 8600.00 0.00 0.18875 11800.00 8600.00 0.00 0.19236 12000.00 8600.00 0.00 0.19347 11600.00 8800.00 0.00 0.19543 11800.00 8800.00 0.00 0.19895 12000.00 8800.00 0.00 0.19989 11600.00 9000.00 0.00 0.20240 11800.00 9000.00 0.00 0.20581 12000.00 9000.00 0.00 0.20657 11600.00 9200.00 0.00 0.20974 11800.00 9200.00 0.00 0.21303 12000.00 9200.00 0.00 0.21358 11600.00 9400.00 0.00 0.21744 11800.00 9400.00 0.00 0.22060 12000.00 9400.00 0.00 0.22094 11600.00 9600.00 0.00 0.22549 11800.00 9600.00 0.00 0.22850 12000.00 9600.00 0.00 0.22860 11600.00 9800.00 0.00 0.23426 11800.00 9800.00 0.00 0.23708 11400.00 10000.00 0.00 0.23810 11600.00 10000.00 0.00 0.24379 11800.00 10000.00 0.00 0.24637 11400.00 10200.00 0.00 0.24802 11600.00 10200.00 0.00 0.25355 11800.00 10200.00 0.00 0.25581 11400.00 10400.00 0.00 0.25802 11600.00 10400.00 0.00 0.26333 11800.00 10400.00 0.00 0.26518 11400.00 10600.00 0.00 0.26816 11600.00 10600.00 0.00 0.27314 11800.00 10600.00 0.00 0.27447 11400.00 10800.00 0.00 0.27812 11600.00 10800.00 0.00 0.28267 11800.00 10800.00 0.00 0.28336 11400.00 11000.00 0.00 0.28801 11600.00 11000.00 0.00 0.29197 11400.00 11200.00 0.00 0.29776 11600.00 11200.00 0.00 0.30081 11400.00 11400.00 0.00 0.30549 11600.00 11400.00 0.00 0.30723 11400.00 11600.00 0.00 0.30877 11600.00 11600.00 0.00 0.30897 11400.00 11600.00 10.00 0.30905 11400.00 11600.00 15.00 0.30915 11400.00 11600.00 20.00 0.30922 11400.00 11600.00 25.00 0.30925 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 11480.57 11626.35 28.65 0.30977 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/160.ctf NX, NY, NZ: 360 360 1 MODE : real