CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/161.mrc Output diagnostic file name Micrographs/161.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/161.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 1988.000 8372.000 Mean, RMS : 4328.056 208.8642 TITLE 1: emi2mrc: 161.emi Sun Jan 19 20:39:55 2014 TITLE 2: kV 300, Mag 78000x, Def 17060, Spot 4, Int 55.11%, Obj 88.08%, Dif 73.67% TITLE 3: X 98.129, Y 684.937, Z -34.314, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 0.19597 8200.00 8000.00 0.00 0.20865 8400.00 8000.00 0.00 0.21996 8600.00 8000.00 0.00 0.22958 8800.00 8000.00 0.00 0.23746 9000.00 8000.00 0.00 0.24259 9200.00 8000.00 0.00 0.24437 8800.00 8200.00 0.00 0.24617 9000.00 8200.00 0.00 0.25082 9200.00 8200.00 0.00 0.25201 8800.00 8400.00 0.00 0.25379 9000.00 8400.00 0.00 0.25797 9200.00 8400.00 0.00 0.25853 8800.00 8600.00 0.00 0.26115 9000.00 8600.00 0.00 0.26475 8800.00 8800.00 0.00 0.26800 9000.00 8800.00 0.00 0.27071 8800.00 9000.00 0.00 0.27260 9000.00 9000.00 0.00 0.27419 9000.00 9200.00 0.00 0.27447 9000.00 9200.00 5.00 0.27460 9000.00 9200.00 10.00 0.27469 9000.00 9200.00 15.00 0.27472 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8919.73 9162.79 17.48 0.27520 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/161.ctf NX, NY, NZ: 360 360 1 MODE : real