CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/162.mrc Output diagnostic file name Micrographs/162.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/162.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2262.000 8298.000 Mean, RMS : 4480.494 212.8102 TITLE 1: emi2mrc: 162.emi Sun Jan 19 20:40:47 2014 TITLE 2: kV 300, Mag 78000x, Def 17060, Spot 4, Int 55.11%, Obj 88.08%, Dif 73.67% TITLE 3: X 100.127, Y 687.508, Z -34.314, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 119 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 0.09535 8200.00 8000.00 0.00 0.10227 8400.00 8000.00 0.00 0.10962 8600.00 8000.00 0.00 0.11779 8800.00 8000.00 0.00 0.12695 9000.00 8000.00 0.00 0.13660 9200.00 8000.00 0.00 0.14636 9400.00 8000.00 0.00 0.15630 9600.00 8000.00 0.00 0.16649 9800.00 8000.00 0.00 0.17707 10000.00 8000.00 0.00 0.18803 10200.00 8000.00 0.00 0.19839 10400.00 8000.00 0.00 0.20649 10600.00 8000.00 0.00 0.21110 10800.00 8000.00 0.00 0.21178 10400.00 8200.00 0.00 0.21227 10600.00 8200.00 0.00 0.21680 10800.00 8200.00 0.00 0.21729 10400.00 8400.00 0.00 0.21835 10600.00 8400.00 0.00 0.22276 10800.00 8400.00 0.00 0.22304 10400.00 8600.00 0.00 0.22543 10600.00 8600.00 0.00 0.22969 10400.00 8800.00 0.00 0.23339 10600.00 8800.00 0.00 0.23742 10400.00 9000.00 0.00 0.24146 10600.00 9000.00 0.00 0.24521 10400.00 9200.00 0.00 0.24927 10600.00 9200.00 0.00 0.25267 10400.00 9400.00 0.00 0.25650 10600.00 9400.00 0.00 0.25950 10400.00 9600.00 0.00 0.26321 10600.00 9600.00 0.00 0.26573 10400.00 9800.00 0.00 0.27036 10600.00 9800.00 0.00 0.27223 10400.00 10000.00 0.00 0.27815 10600.00 10000.00 0.00 0.27906 10200.00 10200.00 0.00 0.27967 10400.00 10200.00 0.00 0.28494 10200.00 10400.00 0.00 0.28508 10400.00 10400.00 0.00 0.28908 10400.00 10600.00 0.00 0.28970 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10408.94 10528.13 3.30 0.28994 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/162.ctf NX, NY, NZ: 360 360 1 MODE : real