CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/163.mrc Output diagnostic file name Micrographs/163.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/163.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2436.000 9074.000 Mean, RMS : 4481.961 215.2864 TITLE 1: emi2mrc: 163.emi Sun Jan 19 20:41:36 2014 TITLE 2: kV 300, Mag 78000x, Def 17060, Spot 4, Int 55.11%, Obj 88.08%, Dif 73.67% TITLE 3: X 101.592, Y 689.457, Z -34.314, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 0.06430 8200.00 8000.00 0.00 0.06942 8400.00 8000.00 0.00 0.07403 8600.00 8000.00 0.00 0.07865 8800.00 8000.00 0.00 0.08408 9000.00 8000.00 0.00 0.09069 9200.00 8000.00 0.00 0.09842 9400.00 8000.00 0.00 0.10686 9600.00 8000.00 0.00 0.11531 9800.00 8000.00 0.00 0.12344 10000.00 8000.00 0.00 0.13172 10200.00 8000.00 0.00 0.14066 10400.00 8000.00 0.00 0.15042 10600.00 8000.00 0.00 0.16067 10800.00 8000.00 0.00 0.17033 11000.00 8000.00 0.00 0.17801 11200.00 8000.00 0.00 0.18296 11400.00 8000.00 0.00 0.18513 11200.00 8200.00 0.00 0.18863 11400.00 8200.00 0.00 0.19064 11200.00 8400.00 0.00 0.19431 11400.00 8400.00 0.00 0.19616 11200.00 8600.00 0.00 0.20072 11400.00 8600.00 0.00 0.20238 11000.00 8800.00 0.00 0.20351 11200.00 8800.00 0.00 0.20803 11400.00 8800.00 0.00 0.20944 11000.00 9000.00 0.00 0.21145 11200.00 9000.00 0.00 0.21578 11400.00 9000.00 0.00 0.21690 11000.00 9200.00 0.00 0.21945 11200.00 9200.00 0.00 0.22354 11400.00 9200.00 0.00 0.22433 11000.00 9400.00 0.00 0.22697 11200.00 9400.00 0.00 0.23081 11400.00 9400.00 0.00 0.23123 11000.00 9600.00 0.00 0.23392 11200.00 9600.00 0.00 0.23748 11400.00 9600.00 0.00 0.23751 11000.00 9800.00 0.00 0.24131 11200.00 9800.00 0.00 0.24449 11000.00 10000.00 0.00 0.24999 11200.00 10000.00 0.00 0.25261 10800.00 10200.00 0.00 0.25339 11000.00 10200.00 0.00 0.25972 11200.00 10200.00 0.00 0.26152 10800.00 10400.00 0.00 0.26437 11000.00 10400.00 0.00 0.26975 11200.00 10400.00 0.00 0.27043 10800.00 10600.00 0.00 0.27469 11000.00 10600.00 0.00 0.27869 10800.00 10800.00 0.00 0.28203 11000.00 10800.00 0.00 0.28439 10800.00 11000.00 0.00 0.28524 11000.00 11000.00 0.00 0.28613 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10917.92 11065.35 20.66 0.28654 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/163.ctf NX, NY, NZ: 360 360 1 MODE : real