CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/165.mrc Output diagnostic file name Micrographs/165.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/165.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2602.000 7938.000 Mean, RMS : 4474.872 214.6846 TITLE 1: emi2mrc: 165.emi Sun Jan 19 20:43:05 2014 TITLE 2: kV 300, Mag 78000x, Def 17060, Spot 4, Int 55.11%, Obj 88.08%, Dif 73.67% TITLE 3: X 105.252, Y 693.737, Z -34.314, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 0.03462 8200.00 8000.00 0.00 0.03673 8400.00 8000.00 0.00 0.03883 8600.00 8000.00 0.00 0.04103 8800.00 8000.00 0.00 0.04352 9000.00 8000.00 0.00 0.04636 9200.00 8000.00 0.00 0.04959 9400.00 8000.00 0.00 0.05324 9600.00 8000.00 0.00 0.05715 9800.00 8000.00 0.00 0.06124 10000.00 8000.00 0.00 0.06565 10200.00 8000.00 0.00 0.07052 10400.00 8000.00 0.00 0.07592 10600.00 8000.00 0.00 0.08191 10800.00 8000.00 0.00 0.08831 11000.00 8000.00 0.00 0.09482 11200.00 8000.00 0.00 0.10131 11400.00 8000.00 0.00 0.10787 11600.00 8000.00 0.00 0.11464 11800.00 8000.00 0.00 0.12194 12000.00 8000.00 0.00 0.12995 12200.00 8000.00 0.00 0.13846 12400.00 8000.00 0.00 0.14692 12600.00 8000.00 0.00 0.15456 12800.00 8000.00 0.00 0.16048 13000.00 8000.00 0.00 0.16405 13200.00 8000.00 0.00 0.16523 13000.00 8200.00 0.00 0.16734 13200.00 8200.00 0.00 0.16842 13000.00 8400.00 0.00 0.17071 13200.00 8400.00 0.00 0.17167 13000.00 8600.00 0.00 0.17466 13200.00 8600.00 0.00 0.17548 12800.00 8800.00 0.00 0.17592 13000.00 8800.00 0.00 0.17919 13200.00 8800.00 0.00 0.17985 12800.00 9000.00 0.00 0.18081 13000.00 9000.00 0.00 0.18396 13200.00 9000.00 0.00 0.18444 12800.00 9200.00 0.00 0.18573 13000.00 9200.00 0.00 0.18874 13200.00 9200.00 0.00 0.18902 12800.00 9400.00 0.00 0.19044 13000.00 9400.00 0.00 0.19330 13200.00 9400.00 0.00 0.19338 12800.00 9600.00 0.00 0.19491 13000.00 9600.00 0.00 0.19759 12800.00 9800.00 0.00 0.19960 13000.00 9800.00 0.00 0.20207 12800.00 10000.00 0.00 0.20480 13000.00 10000.00 0.00 0.20705 12800.00 10200.00 0.00 0.21024 13000.00 10200.00 0.00 0.21225 12800.00 10400.00 0.00 0.21569 13000.00 10400.00 0.00 0.21744 12800.00 10600.00 0.00 0.22096 13000.00 10600.00 0.00 0.22244 12800.00 10800.00 0.00 0.22583 13000.00 10800.00 0.00 0.22705 12800.00 11000.00 0.00 0.23061 13000.00 11000.00 0.00 0.23155 12600.00 11200.00 0.00 0.23178 12800.00 11200.00 0.00 0.23574 13000.00 11200.00 0.00 0.23636 12600.00 11400.00 0.00 0.23731 12800.00 11400.00 0.00 0.24097 13000.00 11400.00 0.00 0.24125 12600.00 11600.00 0.00 0.24259 12800.00 11600.00 0.00 0.24593 12600.00 11800.00 0.00 0.24750 12800.00 11800.00 0.00 0.25051 12600.00 12000.00 0.00 0.25172 12800.00 12000.00 0.00 0.25442 12600.00 12200.00 0.00 0.25534 12800.00 12200.00 0.00 0.25777 12600.00 12400.00 0.00 0.25918 12800.00 12400.00 0.00 0.26127 12600.00 12600.00 0.00 0.26322 12800.00 12600.00 0.00 0.26474 12600.00 12800.00 0.00 0.26627 12800.00 12800.00 0.00 0.26702 12600.00 13000.00 0.00 0.26756 12600.00 13000.00 5.00 0.26767 12600.00 13000.00 10.00 0.26772 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 12687.51 13000.00 6.65 0.26810 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/165.ctf NX, NY, NZ: 360 360 1 MODE : real