CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/168.mrc Output diagnostic file name Micrographs/168.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/168.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2228.000 8090.000 Mean, RMS : 4474.261 217.9025 TITLE 1: emi2mrc: 168.emi Sun Jan 19 20:38:30 2014 TITLE 2: kV 300, Mag 78000x, Def 17060, Spot 4, Int 55.11%, Obj 88.08%, Dif 73.67% TITLE 3: X 99.026, Y 690.365, Z -34.314, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 0.05824 8200.00 8000.00 0.00 0.06178 8400.00 8000.00 0.00 0.06527 8600.00 8000.00 0.00 0.06898 8800.00 8000.00 0.00 0.07325 9000.00 8000.00 0.00 0.07809 9200.00 8000.00 0.00 0.08353 9400.00 8000.00 0.00 0.08965 9600.00 8000.00 0.00 0.09626 9800.00 8000.00 0.00 0.10321 10000.00 8000.00 0.00 0.11050 10200.00 8000.00 0.00 0.11805 10400.00 8000.00 0.00 0.12571 10600.00 8000.00 0.00 0.13360 10800.00 8000.00 0.00 0.14184 11000.00 8000.00 0.00 0.15032 11200.00 8000.00 0.00 0.15892 11400.00 8000.00 0.00 0.16711 11600.00 8000.00 0.00 0.17389 11800.00 8000.00 0.00 0.17844 12000.00 8000.00 0.00 0.18048 11800.00 8200.00 0.00 0.18368 12000.00 8200.00 0.00 0.18564 11800.00 8400.00 0.00 0.18921 12000.00 8400.00 0.00 0.19107 11800.00 8600.00 0.00 0.19548 12000.00 8600.00 0.00 0.19722 11600.00 8800.00 0.00 0.19798 11800.00 8800.00 0.00 0.20240 12000.00 8800.00 0.00 0.20399 11600.00 9000.00 0.00 0.20517 11800.00 9000.00 0.00 0.20950 12000.00 9000.00 0.00 0.21091 11600.00 9200.00 0.00 0.21239 11800.00 9200.00 0.00 0.21660 12000.00 9200.00 0.00 0.21781 11600.00 9400.00 0.00 0.21954 11800.00 9400.00 0.00 0.22363 12000.00 9400.00 0.00 0.22463 11600.00 9600.00 0.00 0.22675 11800.00 9600.00 0.00 0.23070 12000.00 9600.00 0.00 0.23148 11600.00 9800.00 0.00 0.23466 11800.00 9800.00 0.00 0.23845 12000.00 9800.00 0.00 0.23897 11600.00 10000.00 0.00 0.24353 11800.00 10000.00 0.00 0.24712 12000.00 10000.00 0.00 0.24733 11600.00 10200.00 0.00 0.25280 11800.00 10200.00 0.00 0.25615 11600.00 10400.00 0.00 0.26206 11800.00 10400.00 0.00 0.26508 11600.00 10600.00 0.00 0.27107 11800.00 10600.00 0.00 0.27365 11600.00 10800.00 0.00 0.27948 11800.00 10800.00 0.00 0.28151 11400.00 11000.00 0.00 0.28219 11600.00 11000.00 0.00 0.28765 11800.00 11000.00 0.00 0.28897 11400.00 11200.00 0.00 0.29118 11600.00 11200.00 0.00 0.29590 11800.00 11200.00 0.00 0.29624 11400.00 11400.00 0.00 0.29924 11600.00 11400.00 0.00 0.30284 11400.00 11600.00 0.00 0.30423 11600.00 11600.00 0.00 0.30645 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 11697.93 11559.09 -73.62 0.30688 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/168.ctf NX, NY, NZ: 360 360 1 MODE : real