CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/170.mrc Output diagnostic file name Micrographs/170.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/170.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2506.000 8266.000 Mean, RMS : 4475.983 222.3881 TITLE 1: emi2mrc: 170.emi Sun Jan 19 20:48:50 2014 TITLE 2: kV 300, Mag 78000x, Def 17060, Spot 4, Int 55.11%, Obj 88.08%, Dif 73.67% TITLE 3: X 116.089, Y 707.863, Z -34.314, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 0.02998 8200.00 8000.00 0.00 0.03121 8400.00 8000.00 0.00 0.03238 8600.00 8000.00 0.00 0.03383 8800.00 8000.00 0.00 0.03576 9000.00 8000.00 0.00 0.03802 9200.00 8000.00 0.00 0.04037 9400.00 8000.00 0.00 0.04270 9600.00 8000.00 0.00 0.04502 9800.00 8000.00 0.00 0.04753 10000.00 8000.00 0.00 0.05059 10200.00 8000.00 0.00 0.05427 10400.00 8000.00 0.00 0.05843 10600.00 8000.00 0.00 0.06286 10800.00 8000.00 0.00 0.06741 11000.00 8000.00 0.00 0.07210 11200.00 8000.00 0.00 0.07715 11400.00 8000.00 0.00 0.08275 11600.00 8000.00 0.00 0.08873 11800.00 8000.00 0.00 0.09479 12000.00 8000.00 0.00 0.10068 12200.00 8000.00 0.00 0.10634 12400.00 8000.00 0.00 0.11212 12600.00 8000.00 0.00 0.11851 12800.00 8000.00 0.00 0.12563 13000.00 8000.00 0.00 0.13304 13200.00 8000.00 0.00 0.13986 13400.00 8000.00 0.00 0.14511 13600.00 8000.00 0.00 0.14827 13800.00 8000.00 0.00 0.14957 14000.00 8000.00 0.00 0.14962 13600.00 8200.00 0.00 0.15133 13800.00 8200.00 0.00 0.15260 14000.00 8200.00 0.00 0.15261 13600.00 8400.00 0.00 0.15438 13800.00 8400.00 0.00 0.15561 13600.00 8600.00 0.00 0.15786 13800.00 8600.00 0.00 0.15905 13600.00 8800.00 0.00 0.16179 13800.00 8800.00 0.00 0.16292 13600.00 9000.00 0.00 0.16590 13800.00 9000.00 0.00 0.16697 13400.00 9200.00 0.00 0.16707 13600.00 9200.00 0.00 0.17007 13800.00 9200.00 0.00 0.17106 13400.00 9400.00 0.00 0.17124 13600.00 9400.00 0.00 0.17419 13800.00 9400.00 0.00 0.17509 13400.00 9600.00 0.00 0.17542 13600.00 9600.00 0.00 0.17832 13800.00 9600.00 0.00 0.17912 13400.00 9800.00 0.00 0.18011 13600.00 9800.00 0.00 0.18293 13800.00 9800.00 0.00 0.18362 13400.00 10000.00 0.00 0.18554 13600.00 10000.00 0.00 0.18828 13800.00 10000.00 0.00 0.18884 13400.00 10200.00 0.00 0.19143 13600.00 10200.00 0.00 0.19407 13800.00 10200.00 0.00 0.19449 13400.00 10400.00 0.00 0.19749 13600.00 10400.00 0.00 0.20003 13800.00 10400.00 0.00 0.20028 13400.00 10600.00 0.00 0.20360 13600.00 10600.00 0.00 0.20602 13800.00 10600.00 0.00 0.20610 13400.00 10800.00 0.00 0.20968 13600.00 10800.00 0.00 0.21197 13400.00 11000.00 0.00 0.21616 13600.00 11000.00 0.00 0.21829 13200.00 11200.00 0.00 0.21835 13400.00 11200.00 0.00 0.22351 13600.00 11200.00 0.00 0.22543 13200.00 11400.00 0.00 0.22638 13400.00 11400.00 0.00 0.23141 13600.00 11400.00 0.00 0.23305 13200.00 11600.00 0.00 0.23440 13400.00 11600.00 0.00 0.23927 13600.00 11600.00 0.00 0.24058 13200.00 11800.00 0.00 0.24205 13400.00 11800.00 0.00 0.24673 13600.00 11800.00 0.00 0.24766 13200.00 12000.00 0.00 0.24913 13400.00 12000.00 0.00 0.25359 13600.00 12000.00 0.00 0.25412 13200.00 12200.00 0.00 0.25616 13400.00 12200.00 0.00 0.26036 13600.00 12200.00 0.00 0.26042 13200.00 12400.00 0.00 0.26437 13400.00 12400.00 0.00 0.26815 13200.00 12600.00 0.00 0.27393 13400.00 12600.00 0.00 0.27698 13200.00 12800.00 0.00 0.28361 13400.00 12800.00 0.00 0.28558 13000.00 13000.00 0.00 0.28603 13200.00 13000.00 0.00 0.29184 13400.00 13000.00 0.00 0.29237 13000.00 13200.00 0.00 0.29240 13200.00 13200.00 0.00 0.29671 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 13222.12 13291.13 19.82 0.29738 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/170.ctf NX, NY, NZ: 360 360 1 MODE : real