CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/174.mrc Output diagnostic file name Micrographs/174.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/174.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2328.000 7872.000 Mean, RMS : 4471.594 218.9580 TITLE 1: emi2mrc: 174.emi Sun Jan 19 20:54:36 2014 TITLE 2: kV 300, Mag 78000x, Def 17060, Spot 4, Int 55.11%, Obj 88.08%, Dif 73.67% TITLE 3: X 109.896, Y 695.074, Z -34.314, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 0.03732 8200.00 8000.00 0.00 0.03962 8400.00 8000.00 0.00 0.04170 8600.00 8000.00 0.00 0.04363 8800.00 8000.00 0.00 0.04563 9000.00 8000.00 0.00 0.04784 9200.00 8000.00 0.00 0.05044 9400.00 8000.00 0.00 0.05360 9600.00 8000.00 0.00 0.05725 9800.00 8000.00 0.00 0.06127 10000.00 8000.00 0.00 0.06568 10200.00 8000.00 0.00 0.07048 10400.00 8000.00 0.00 0.07565 10600.00 8000.00 0.00 0.08131 10800.00 8000.00 0.00 0.08743 11000.00 8000.00 0.00 0.09383 11200.00 8000.00 0.00 0.10049 11400.00 8000.00 0.00 0.10740 11600.00 8000.00 0.00 0.11455 11800.00 8000.00 0.00 0.12203 12000.00 8000.00 0.00 0.12992 12200.00 8000.00 0.00 0.13801 12400.00 8000.00 0.00 0.14593 12600.00 8000.00 0.00 0.15313 12800.00 8000.00 0.00 0.15888 13000.00 8000.00 0.00 0.16264 13200.00 8000.00 0.00 0.16427 13000.00 8200.00 0.00 0.16623 13200.00 8200.00 0.00 0.16778 13000.00 8400.00 0.00 0.16983 13200.00 8400.00 0.00 0.17129 13000.00 8600.00 0.00 0.17391 13200.00 8600.00 0.00 0.17529 13000.00 8800.00 0.00 0.17848 13200.00 8800.00 0.00 0.17978 13000.00 9000.00 0.00 0.18326 13200.00 9000.00 0.00 0.18448 12800.00 9200.00 0.00 0.18473 13000.00 9200.00 0.00 0.18818 13200.00 9200.00 0.00 0.18933 12800.00 9400.00 0.00 0.18974 13000.00 9400.00 0.00 0.19316 13200.00 9400.00 0.00 0.19422 12800.00 9600.00 0.00 0.19484 13000.00 9600.00 0.00 0.19822 13200.00 9600.00 0.00 0.19918 12800.00 9800.00 0.00 0.20049 13000.00 9800.00 0.00 0.20381 13200.00 9800.00 0.00 0.20463 12800.00 10000.00 0.00 0.20690 13000.00 10000.00 0.00 0.21011 13200.00 10000.00 0.00 0.21074 12800.00 10200.00 0.00 0.21369 13000.00 10200.00 0.00 0.21675 13200.00 10200.00 0.00 0.21716 12800.00 10400.00 0.00 0.22055 13000.00 10400.00 0.00 0.22343 13200.00 10400.00 0.00 0.22357 12800.00 10600.00 0.00 0.22732 13000.00 10600.00 0.00 0.22996 12800.00 10800.00 0.00 0.23381 13000.00 10800.00 0.00 0.23620 12800.00 11000.00 0.00 0.24041 13000.00 11000.00 0.00 0.24252 12600.00 11200.00 0.00 0.24277 12800.00 11200.00 0.00 0.24764 13000.00 11200.00 0.00 0.24942 12600.00 11400.00 0.00 0.25069 12800.00 11400.00 0.00 0.25526 13000.00 11400.00 0.00 0.25665 12600.00 11600.00 0.00 0.25859 12800.00 11600.00 0.00 0.26280 13000.00 11600.00 0.00 0.26373 12600.00 11800.00 0.00 0.26623 12800.00 11800.00 0.00 0.26998 13000.00 11800.00 0.00 0.27038 12600.00 12000.00 0.00 0.27299 12800.00 12000.00 0.00 0.27624 12600.00 12200.00 0.00 0.27865 12800.00 12200.00 0.00 0.28138 12600.00 12400.00 0.00 0.28378 12800.00 12400.00 0.00 0.28589 12600.00 12600.00 0.00 0.28814 12800.00 12600.00 0.00 0.28938 12600.00 12800.00 0.00 0.29037 12800.00 12800.00 0.00 0.29054 12600.00 12800.00 20.00 0.29056 12600.00 12800.00 25.00 0.29057 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 12691.24 12829.86 17.54 0.29106 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/174.ctf NX, NY, NZ: 360 360 1 MODE : real