CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/175.mrc Output diagnostic file name Micrographs/175.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/175.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2478.000 8450.000 Mean, RMS : 4477.729 217.2880 TITLE 1: emi2mrc: 175.emi Sun Jan 19 20:55:27 2014 TITLE 2: kV 300, Mag 78000x, Def 17060, Spot 4, Int 55.11%, Obj 88.08%, Dif 73.67% TITLE 3: X 108.356, Y 693.418, Z -34.314, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 0.07357 8200.00 8000.00 0.00 0.07858 8400.00 8000.00 0.00 0.08310 8600.00 8000.00 0.00 0.08783 8800.00 8000.00 0.00 0.09369 9000.00 8000.00 0.00 0.10093 9200.00 8000.00 0.00 0.10932 9400.00 8000.00 0.00 0.11837 9600.00 8000.00 0.00 0.12734 9800.00 8000.00 0.00 0.13604 10000.00 8000.00 0.00 0.14509 10200.00 8000.00 0.00 0.15503 10400.00 8000.00 0.00 0.16580 10600.00 8000.00 0.00 0.17676 10800.00 8000.00 0.00 0.18656 11000.00 8000.00 0.00 0.19376 11200.00 8000.00 0.00 0.19787 11400.00 8000.00 0.00 0.19920 11000.00 8200.00 0.00 0.20003 11200.00 8200.00 0.00 0.20408 11400.00 8200.00 0.00 0.20529 11000.00 8400.00 0.00 0.20639 11200.00 8400.00 0.00 0.21034 11400.00 8400.00 0.00 0.21141 11000.00 8600.00 0.00 0.21355 11200.00 8600.00 0.00 0.21736 11400.00 8600.00 0.00 0.21823 11000.00 8800.00 0.00 0.22165 11200.00 8800.00 0.00 0.22527 11400.00 8800.00 0.00 0.22586 11000.00 9000.00 0.00 0.23026 11200.00 9000.00 0.00 0.23363 11400.00 9000.00 0.00 0.23388 11000.00 9200.00 0.00 0.23900 11200.00 9200.00 0.00 0.24206 11000.00 9400.00 0.00 0.24737 11200.00 9400.00 0.00 0.25013 11000.00 9600.00 0.00 0.25528 11200.00 9600.00 0.00 0.25771 11000.00 9800.00 0.00 0.26366 11200.00 9800.00 0.00 0.26567 10800.00 10000.00 0.00 0.26733 11000.00 10000.00 0.00 0.27324 11200.00 10000.00 0.00 0.27460 10800.00 10200.00 0.00 0.27832 11000.00 10200.00 0.00 0.28352 11200.00 10200.00 0.00 0.28397 10800.00 10400.00 0.00 0.28953 11000.00 10400.00 0.00 0.29363 10800.00 10600.00 0.00 0.29960 11000.00 10600.00 0.00 0.30219 10800.00 10800.00 0.00 0.30623 11000.00 10800.00 0.00 0.30713 10800.00 11000.00 0.00 0.30843 10800.00 11000.00 5.00 0.30853 10800.00 11000.00 10.00 0.30861 10800.00 11000.00 15.00 0.30867 10800.00 11000.00 20.00 0.30870 10800.00 11000.00 25.00 0.30870 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10866.09 10994.05 19.30 0.30895 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/175.ctf NX, NY, NZ: 360 360 1 MODE : real