CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/176.mrc Output diagnostic file name Micrographs/176.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/176.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2280.000 7776.000 Mean, RMS : 4478.851 215.0853 TITLE 1: emi2mrc: 176.emi Sun Jan 19 20:56:08 2014 TITLE 2: kV 300, Mag 78000x, Def 17060, Spot 4, Int 55.11%, Obj 88.08%, Dif 73.67% TITLE 3: X 106.473, Y 691.098, Z -34.314, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 0.10885 8200.00 8000.00 0.00 0.11734 8400.00 8000.00 0.00 0.12563 8600.00 8000.00 0.00 0.13396 8800.00 8000.00 0.00 0.14304 9000.00 8000.00 0.00 0.15327 9200.00 8000.00 0.00 0.16500 9400.00 8000.00 0.00 0.17832 9600.00 8000.00 0.00 0.19230 9800.00 8000.00 0.00 0.20552 10000.00 8000.00 0.00 0.21678 10200.00 8000.00 0.00 0.22475 10400.00 8000.00 0.00 0.22846 10200.00 8200.00 0.00 0.23246 10400.00 8200.00 0.00 0.23587 10200.00 8400.00 0.00 0.24026 10400.00 8400.00 0.00 0.24333 10200.00 8600.00 0.00 0.24876 10400.00 8600.00 0.00 0.25144 10200.00 8800.00 0.00 0.25782 10400.00 8800.00 0.00 0.26004 10000.00 9000.00 0.00 0.26032 10200.00 9000.00 0.00 0.26670 10400.00 9000.00 0.00 0.26837 10000.00 9200.00 0.00 0.26919 10200.00 9200.00 0.00 0.27503 10400.00 9200.00 0.00 0.27606 10000.00 9400.00 0.00 0.27701 10200.00 9400.00 0.00 0.28226 10400.00 9400.00 0.00 0.28258 10000.00 9600.00 0.00 0.28329 10200.00 9600.00 0.00 0.28799 10000.00 9800.00 0.00 0.28887 10200.00 9800.00 0.00 0.29298 10000.00 10000.00 0.00 0.29427 10200.00 10000.00 0.00 0.29758 10000.00 10200.00 0.00 0.29826 10200.00 10200.00 0.00 0.30042 10200.00 10400.00 0.00 0.30047 10200.00 10400.00 5.00 0.30051 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10140.48 10332.09 9.24 0.30106 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/176.ctf NX, NY, NZ: 360 360 1 MODE : real