CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/181.mrc Output diagnostic file name Micrographs/181.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/181.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2058.000 7662.000 Mean, RMS : 4505.494 217.5053 TITLE 1: emi2mrc: 181.emi Sun Jan 19 21:01:15 2014 TITLE 2: kV 300, Mag 78000x, Def 14600, Spot 4, Int 55.11%, Obj 88.06%, Dif 73.67% TITLE 3: X 104.188, Y 684.031, Z -34.314, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 0.03406 8200.00 8000.00 0.00 0.03728 8400.00 8000.00 0.00 0.04025 8600.00 8000.00 0.00 0.04317 8800.00 8000.00 0.00 0.04643 9000.00 8000.00 0.00 0.05023 9200.00 8000.00 0.00 0.05453 9400.00 8000.00 0.00 0.05920 9600.00 8000.00 0.00 0.06394 9800.00 8000.00 0.00 0.06872 10000.00 8000.00 0.00 0.07391 10200.00 8000.00 0.00 0.07986 10400.00 8000.00 0.00 0.08666 10600.00 8000.00 0.00 0.09418 10800.00 8000.00 0.00 0.10197 11000.00 8000.00 0.00 0.10962 11200.00 8000.00 0.00 0.11722 11400.00 8000.00 0.00 0.12513 11600.00 8000.00 0.00 0.13361 11800.00 8000.00 0.00 0.14260 12000.00 8000.00 0.00 0.15153 12200.00 8000.00 0.00 0.15939 12400.00 8000.00 0.00 0.16532 12600.00 8000.00 0.00 0.16898 12800.00 8000.00 0.00 0.17045 12600.00 8200.00 0.00 0.17255 12800.00 8200.00 0.00 0.17391 12600.00 8400.00 0.00 0.17620 12800.00 8400.00 0.00 0.17743 12600.00 8600.00 0.00 0.18039 12800.00 8600.00 0.00 0.18149 12400.00 8800.00 0.00 0.18181 12600.00 8800.00 0.00 0.18510 12800.00 8800.00 0.00 0.18606 12400.00 9000.00 0.00 0.18677 12600.00 9000.00 0.00 0.18995 12800.00 9000.00 0.00 0.19074 12400.00 9200.00 0.00 0.19166 12600.00 9200.00 0.00 0.19472 12800.00 9200.00 0.00 0.19536 12400.00 9400.00 0.00 0.19632 12600.00 9400.00 0.00 0.19927 12800.00 9400.00 0.00 0.19974 12400.00 9600.00 0.00 0.20083 12600.00 9600.00 0.00 0.20365 12800.00 9600.00 0.00 0.20397 12400.00 9800.00 0.00 0.20579 12600.00 9800.00 0.00 0.20848 12800.00 9800.00 0.00 0.20861 12400.00 10000.00 0.00 0.21159 12600.00 10000.00 0.00 0.21411 12400.00 10200.00 0.00 0.21792 12600.00 10200.00 0.00 0.22023 12400.00 10400.00 0.00 0.22442 12600.00 10400.00 0.00 0.22648 12400.00 10600.00 0.00 0.23074 12600.00 10600.00 0.00 0.23252 12400.00 10800.00 0.00 0.23657 12600.00 10800.00 0.00 0.23804 12400.00 11000.00 0.00 0.24231 12600.00 11000.00 0.00 0.24341 12200.00 11200.00 0.00 0.24462 12400.00 11200.00 0.00 0.24863 12600.00 11200.00 0.00 0.24922 12200.00 11400.00 0.00 0.25186 12400.00 11400.00 0.00 0.25528 12200.00 11600.00 0.00 0.25903 12400.00 11600.00 0.00 0.26167 12200.00 11800.00 0.00 0.26557 12400.00 11800.00 0.00 0.26722 12200.00 12000.00 0.00 0.27031 12400.00 12000.00 0.00 0.27089 12200.00 12200.00 0.00 0.27255 12200.00 12400.00 0.00 0.27314 12200.00 12400.00 5.00 0.27320 12200.00 12400.00 10.00 0.27324 12200.00 12400.00 15.00 0.27328 12200.00 12400.00 20.00 0.27331 12200.00 12400.00 25.00 0.27332 12200.00 12400.00 30.00 0.27332 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 12224.22 12421.15 22.50 0.27336 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/181.ctf NX, NY, NZ: 360 360 1 MODE : real