CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/186.mrc Output diagnostic file name Micrographs/186.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/186.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2392.000 8342.000 Mean, RMS : 4443.028 220.6092 TITLE 1: emi2mrc: 186.emi Sun Jan 19 21:06:01 2014 TITLE 2: kV 300, Mag 78000x, Def 14600, Spot 4, Int 55.12%, Obj 88.06%, Dif 73.67% TITLE 3: X 113.902, Y 696.412, Z -34.314, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.01093 8200.00 8000.00 0.00 -0.01045 8400.00 8000.00 0.00 -0.01007 8600.00 8000.00 0.00 -0.00989 8800.00 8000.00 0.00 -0.00987 9000.00 8000.00 0.00 -0.00984 9200.00 8000.00 0.00 -0.00967 9400.00 8000.00 0.00 -0.00931 9600.00 8000.00 0.00 -0.00880 9800.00 8000.00 0.00 -0.00827 10000.00 8000.00 0.00 -0.00782 10200.00 8000.00 0.00 -0.00744 10400.00 8000.00 0.00 -0.00704 10600.00 8000.00 0.00 -0.00650 10800.00 8000.00 0.00 -0.00574 11000.00 8000.00 0.00 -0.00475 11200.00 8000.00 0.00 -0.00361 11400.00 8000.00 0.00 -0.00237 11600.00 8000.00 0.00 -0.00108 11800.00 8000.00 0.00 0.00025 12000.00 8000.00 0.00 0.00162 12200.00 8000.00 0.00 0.00304 12400.00 8000.00 0.00 0.00452 12600.00 8000.00 0.00 0.00607 12800.00 8000.00 0.00 0.00769 13000.00 8000.00 0.00 0.00939 13200.00 8000.00 0.00 0.01114 13400.00 8000.00 0.00 0.01293 13600.00 8000.00 0.00 0.01478 13800.00 8000.00 0.00 0.01673 14000.00 8000.00 0.00 0.01883 14200.00 8000.00 0.00 0.02112 14400.00 8000.00 0.00 0.02358 14600.00 8000.00 0.00 0.02614 14800.00 8000.00 0.00 0.02872 15000.00 8000.00 0.00 0.03129 15200.00 8000.00 0.00 0.03386 15400.00 8000.00 0.00 0.03650 15600.00 8000.00 0.00 0.03925 15800.00 8000.00 0.00 0.04215 16000.00 8000.00 0.00 0.04517 16200.00 8000.00 0.00 0.04829 16400.00 8000.00 0.00 0.05152 16600.00 8000.00 0.00 0.05483 16800.00 8000.00 0.00 0.05821 17000.00 8000.00 0.00 0.06164 17200.00 8000.00 0.00 0.06508 17400.00 8000.00 0.00 0.06853 17600.00 8000.00 0.00 0.07208 17800.00 8000.00 0.00 0.07581 18000.00 8000.00 0.00 0.07972 18200.00 8000.00 0.00 0.08372 18400.00 8000.00 0.00 0.08758 18600.00 8000.00 0.00 0.09103 18800.00 8000.00 0.00 0.09386 19000.00 8000.00 0.00 0.09593 19200.00 8000.00 0.00 0.09724 19400.00 8000.00 0.00 0.09790 19600.00 8000.00 0.00 0.09808 19200.00 8200.00 0.00 0.09844 19400.00 8200.00 0.00 0.09908 19600.00 8200.00 0.00 0.09923 19200.00 8400.00 0.00 0.09967 19400.00 8400.00 0.00 0.10029 19600.00 8400.00 0.00 0.10041 19200.00 8600.00 0.00 0.10107 19400.00 8600.00 0.00 0.10166 19600.00 8600.00 0.00 0.10175 19200.00 8800.00 0.00 0.10259 19400.00 8800.00 0.00 0.10314 19600.00 8800.00 0.00 0.10320 19200.00 9000.00 0.00 0.10405 19400.00 9000.00 0.00 0.10457 19600.00 9000.00 0.00 0.10459 19200.00 9200.00 0.00 0.10541 19400.00 9200.00 0.00 0.10589 19200.00 9400.00 0.00 0.10664 19400.00 9400.00 0.00 0.10709 19200.00 9600.00 0.00 0.10784 19400.00 9600.00 0.00 0.10827 19200.00 9800.00 0.00 0.10925 19400.00 9800.00 0.00 0.10968 19000.00 10000.00 0.00 0.10987 19200.00 10000.00 0.00 0.11098 19400.00 10000.00 0.00 0.11142 19000.00 10200.00 0.00 0.11181 19200.00 10200.00 0.00 0.11294 19400.00 10200.00 0.00 0.11340 19000.00 10400.00 0.00 0.11386 19200.00 10400.00 0.00 0.11501 19400.00 10400.00 0.00 0.11547 19000.00 10600.00 0.00 0.11591 19200.00 10600.00 0.00 0.11706 19400.00 10600.00 0.00 0.11750 19000.00 10800.00 0.00 0.11786 19200.00 10800.00 0.00 0.11901 19400.00 10800.00 0.00 0.11941 19000.00 11000.00 0.00 0.11983 19200.00 11000.00 0.00 0.12095 19400.00 11000.00 0.00 0.12130 19000.00 11200.00 0.00 0.12193 19200.00 11200.00 0.00 0.12302 19400.00 11200.00 0.00 0.12332 19000.00 11400.00 0.00 0.12409 19200.00 11400.00 0.00 0.12514 19400.00 11400.00 0.00 0.12537 19000.00 11600.00 0.00 0.12621 19200.00 11600.00 0.00 0.12722 19400.00 11600.00 0.00 0.12737 19000.00 11800.00 0.00 0.12826 19200.00 11800.00 0.00 0.12921 19400.00 11800.00 0.00 0.12928 19000.00 12000.00 0.00 0.13020 19200.00 12000.00 0.00 0.13108 19000.00 12200.00 0.00 0.13213 19200.00 12200.00 0.00 0.13294 19000.00 12400.00 0.00 0.13431 19200.00 12400.00 0.00 0.13505 19000.00 12600.00 0.00 0.13675 19200.00 12600.00 0.00 0.13740 18800.00 12800.00 0.00 0.13757 19000.00 12800.00 0.00 0.13928 19200.00 12800.00 0.00 0.13983 18800.00 13000.00 0.00 0.14019 19000.00 13000.00 0.00 0.14181 19200.00 13000.00 0.00 0.14227 18800.00 13200.00 0.00 0.14271 19000.00 13200.00 0.00 0.14426 19200.00 13200.00 0.00 0.14464 18800.00 13400.00 0.00 0.14517 19000.00 13400.00 0.00 0.14666 19200.00 13400.00 0.00 0.14699 18800.00 13600.00 0.00 0.14783 19000.00 13600.00 0.00 0.14927 19200.00 13600.00 0.00 0.14955 18800.00 13800.00 0.00 0.15084 19000.00 13800.00 0.00 0.15222 19200.00 13800.00 0.00 0.15246 18800.00 14000.00 0.00 0.15402 19000.00 14000.00 0.00 0.15535 19200.00 14000.00 0.00 0.15553 18800.00 14200.00 0.00 0.15722 19000.00 14200.00 0.00 0.15849 19200.00 14200.00 0.00 0.15861 18800.00 14400.00 0.00 0.16031 19000.00 14400.00 0.00 0.16151 19200.00 14400.00 0.00 0.16157 18800.00 14600.00 0.00 0.16317 19000.00 14600.00 0.00 0.16431 18800.00 14800.00 0.00 0.16599 19000.00 14800.00 0.00 0.16708 18800.00 15000.00 0.00 0.16913 19000.00 15000.00 0.00 0.17016 18600.00 15200.00 0.00 0.17030 18800.00 15200.00 0.00 0.17257 19000.00 15200.00 0.00 0.17355 18600.00 15400.00 0.00 0.17391 18800.00 15400.00 0.00 0.17614 19000.00 15400.00 0.00 0.17707 18600.00 15600.00 0.00 0.17755 18800.00 15600.00 0.00 0.17975 19000.00 15600.00 0.00 0.18061 18600.00 15800.00 0.00 0.18105 18800.00 15800.00 0.00 0.18321 19000.00 15800.00 0.00 0.18399 18600.00 16000.00 0.00 0.18442 18800.00 16000.00 0.00 0.18651 19000.00 16000.00 0.00 0.18719 18600.00 16200.00 0.00 0.18799 18800.00 16200.00 0.00 0.18997 19000.00 16200.00 0.00 0.19050 18600.00 16400.00 0.00 0.19183 18800.00 16400.00 0.00 0.19366 19000.00 16400.00 0.00 0.19398 18600.00 16600.00 0.00 0.19571 18800.00 16600.00 0.00 0.19734 19000.00 16600.00 0.00 0.19743 18600.00 16800.00 0.00 0.19945 18800.00 16800.00 0.00 0.20086 18600.00 17000.00 0.00 0.20285 18800.00 17000.00 0.00 0.20403 18600.00 17200.00 0.00 0.20587 18800.00 17200.00 0.00 0.20685 18600.00 17400.00 0.00 0.20899 18800.00 17400.00 0.00 0.20974 18400.00 17600.00 0.00 0.21024 18600.00 17600.00 0.00 0.21264 18800.00 17600.00 0.00 0.21309 18400.00 17800.00 0.00 0.21456 18600.00 17800.00 0.00 0.21662 18800.00 17800.00 0.00 0.21667 18400.00 18000.00 0.00 0.21894 18600.00 18000.00 0.00 0.22052 18400.00 18200.00 0.00 0.22279 18600.00 18200.00 0.00 0.22375 18400.00 18400.00 0.00 0.22511 18600.00 18400.00 0.00 0.22536 18400.00 18600.00 0.00 0.22538 18400.00 18600.00 5.00 0.22547 18400.00 18600.00 10.00 0.22556 18400.00 18600.00 15.00 0.22565 18400.00 18600.00 20.00 0.22572 18400.00 18600.00 25.00 0.22579 18400.00 18600.00 30.00 0.22584 18400.00 18600.00 35.00 0.22588 18400.00 18600.00 40.00 0.22590 18400.00 18600.00 45.00 0.22590 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 18416.51 18572.45 43.25 0.22591 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/186.ctf NX, NY, NZ: 360 360 1 MODE : real