CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/193.mrc Output diagnostic file name Micrographs/193.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/193.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2484.000 8584.000 Mean, RMS : 4435.419 218.1991 TITLE 1: emi2mrc: 193.emi Sun Jan 19 21:12:42 2014 TITLE 2: kV 300, Mag 78000x, Def 20350, Spot 4, Int 55.12%, Obj 88.07%, Dif 73.67% TITLE 3: X 123.972, Y 704.412, Z -35.048, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 119 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.02737 9400.00 8000.00 0.00 -0.02734 9600.00 8000.00 0.00 -0.02711 9800.00 8000.00 0.00 -0.02679 10000.00 8000.00 0.00 -0.02644 10200.00 8000.00 0.00 -0.02603 10400.00 8000.00 0.00 -0.02547 10600.00 8000.00 0.00 -0.02475 10800.00 8000.00 0.00 -0.02387 11000.00 8000.00 0.00 -0.02287 11200.00 8000.00 0.00 -0.02183 11400.00 8000.00 0.00 -0.02084 11600.00 8000.00 0.00 -0.01991 11800.00 8000.00 0.00 -0.01908 12000.00 8000.00 0.00 -0.01835 12200.00 8000.00 0.00 -0.01768 12400.00 8000.00 0.00 -0.01701 12600.00 8000.00 0.00 -0.01623 12800.00 8000.00 0.00 -0.01524 13000.00 8000.00 0.00 -0.01397 13200.00 8000.00 0.00 -0.01247 13400.00 8000.00 0.00 -0.01081 13600.00 8000.00 0.00 -0.00910 13800.00 8000.00 0.00 -0.00739 14000.00 8000.00 0.00 -0.00564 14200.00 8000.00 0.00 -0.00377 14400.00 8000.00 0.00 -0.00172 14600.00 8000.00 0.00 0.00049 14800.00 8000.00 0.00 0.00282 15000.00 8000.00 0.00 0.00518 15200.00 8000.00 0.00 0.00754 15400.00 8000.00 0.00 0.00991 15600.00 8000.00 0.00 0.01231 15800.00 8000.00 0.00 0.01476 16000.00 8000.00 0.00 0.01726 16200.00 8000.00 0.00 0.01979 16400.00 8000.00 0.00 0.02234 16600.00 8000.00 0.00 0.02489 16800.00 8000.00 0.00 0.02744 17000.00 8000.00 0.00 0.02996 17200.00 8000.00 0.00 0.03246 17400.00 8000.00 0.00 0.03496 17600.00 8000.00 0.00 0.03756 17800.00 8000.00 0.00 0.04034 18000.00 8000.00 0.00 0.04332 18200.00 8000.00 0.00 0.04648 18400.00 8000.00 0.00 0.04969 18600.00 8000.00 0.00 0.05286 18800.00 8000.00 0.00 0.05597 19000.00 8000.00 0.00 0.05910 19200.00 8000.00 0.00 0.06235 19400.00 8000.00 0.00 0.06578 19600.00 8000.00 0.00 0.06932 19800.00 8000.00 0.00 0.07270 20000.00 8000.00 0.00 0.07563 20200.00 8000.00 0.00 0.07783 20400.00 8000.00 0.00 0.07915 20600.00 8000.00 0.00 0.07967 20400.00 8200.00 0.00 0.08006 20600.00 8200.00 0.00 0.08061 20400.00 8400.00 0.00 0.08107 20600.00 8400.00 0.00 0.08163 20400.00 8600.00 0.00 0.08219 20600.00 8600.00 0.00 0.08274 20400.00 8800.00 0.00 0.08329 20600.00 8800.00 0.00 0.08381 20400.00 9000.00 0.00 0.08423 20600.00 9000.00 0.00 0.08471 20400.00 9200.00 0.00 0.08503 20600.00 9200.00 0.00 0.08547 20400.00 9400.00 0.00 0.08577 20600.00 9400.00 0.00 0.08617 20400.00 9600.00 0.00 0.08654 20600.00 9600.00 0.00 0.08692 20400.00 9800.00 0.00 0.08751 20600.00 9800.00 0.00 0.08788 20400.00 10000.00 0.00 0.08870 20600.00 10000.00 0.00 0.08907 20400.00 10200.00 0.00 0.09000 20600.00 10200.00 0.00 0.09039 20400.00 10400.00 0.00 0.09134 20600.00 10400.00 0.00 0.09175 20400.00 10600.00 0.00 0.09274 20600.00 10600.00 0.00 0.09317 20400.00 10800.00 0.00 0.09422 20600.00 10800.00 0.00 0.09467 20400.00 11000.00 0.00 0.09589 20600.00 11000.00 0.00 0.09636 20200.00 11200.00 0.00 0.09655 20400.00 11200.00 0.00 0.09781 20600.00 11200.00 0.00 0.09829 20200.00 11400.00 0.00 0.09856 20400.00 11400.00 0.00 0.09982 20600.00 11400.00 0.00 0.10031 20200.00 11600.00 0.00 0.10047 20400.00 11600.00 0.00 0.10174 20600.00 11600.00 0.00 0.10222 20400.00 11800.00 0.00 0.10348 20600.00 11800.00 0.00 0.10396 20400.00 12000.00 0.00 0.10504 20600.00 12000.00 0.00 0.10550 20400.00 12200.00 0.00 0.10657 20600.00 12200.00 0.00 0.10701 20200.00 12400.00 0.00 0.10701 20400.00 12400.00 0.00 0.10825 20600.00 12400.00 0.00 0.10867 20200.00 12600.00 0.00 0.10887 20400.00 12600.00 0.00 0.11009 20600.00 12600.00 0.00 0.11048 20200.00 12800.00 0.00 0.11075 20400.00 12800.00 0.00 0.11194 20600.00 12800.00 0.00 0.11230 20200.00 13000.00 0.00 0.11258 20400.00 13000.00 0.00 0.11375 20600.00 13000.00 0.00 0.11408 20200.00 13200.00 0.00 0.11432 20400.00 13200.00 0.00 0.11547 20600.00 13200.00 0.00 0.11578 20200.00 13400.00 0.00 0.11604 20400.00 13400.00 0.00 0.11718 20600.00 13400.00 0.00 0.11749 20200.00 13600.00 0.00 0.11797 20400.00 13600.00 0.00 0.11913 20600.00 13600.00 0.00 0.11945 20200.00 13800.00 0.00 0.12025 20400.00 13800.00 0.00 0.12143 20600.00 13800.00 0.00 0.12176 20200.00 14000.00 0.00 0.12273 20400.00 14000.00 0.00 0.12393 20600.00 14000.00 0.00 0.12427 20200.00 14200.00 0.00 0.12528 20400.00 14200.00 0.00 0.12649 20600.00 14200.00 0.00 0.12684 20200.00 14400.00 0.00 0.12779 20400.00 14400.00 0.00 0.12903 20600.00 14400.00 0.00 0.12937 20200.00 14600.00 0.00 0.13024 20400.00 14600.00 0.00 0.13150 20600.00 14600.00 0.00 0.13185 20200.00 14800.00 0.00 0.13282 20400.00 14800.00 0.00 0.13410 20600.00 14800.00 0.00 0.13445 20200.00 15000.00 0.00 0.13576 20400.00 15000.00 0.00 0.13704 20600.00 15000.00 0.00 0.13736 20200.00 15200.00 0.00 0.13896 20400.00 15200.00 0.00 0.14022 20600.00 15200.00 0.00 0.14050 20200.00 15400.00 0.00 0.14222 20400.00 15400.00 0.00 0.14345 20600.00 15400.00 0.00 0.14366 20200.00 15600.00 0.00 0.14544 20400.00 15600.00 0.00 0.14661 20600.00 15600.00 0.00 0.14673 20200.00 15800.00 0.00 0.14845 20400.00 15800.00 0.00 0.14954 20600.00 15800.00 0.00 0.14956 20200.00 16000.00 0.00 0.15131 20400.00 16000.00 0.00 0.15231 20200.00 16200.00 0.00 0.15431 20400.00 16200.00 0.00 0.15521 20000.00 16400.00 0.00 0.15544 20200.00 16400.00 0.00 0.15753 20400.00 16400.00 0.00 0.15833 20000.00 16600.00 0.00 0.15876 20200.00 16600.00 0.00 0.16078 20400.00 16600.00 0.00 0.16147 20000.00 16800.00 0.00 0.16199 20200.00 16800.00 0.00 0.16393 20400.00 16800.00 0.00 0.16452 20000.00 17000.00 0.00 0.16498 20200.00 17000.00 0.00 0.16686 20400.00 17000.00 0.00 0.16736 20000.00 17200.00 0.00 0.16771 20200.00 17200.00 0.00 0.16952 20400.00 17200.00 0.00 0.16992 20000.00 17400.00 0.00 0.17045 20200.00 17400.00 0.00 0.17220 20400.00 17400.00 0.00 0.17248 20000.00 17600.00 0.00 0.17348 20200.00 17600.00 0.00 0.17513 20400.00 17600.00 0.00 0.17528 20000.00 17800.00 0.00 0.17670 20200.00 17800.00 0.00 0.17822 20000.00 18000.00 0.00 0.17994 20200.00 18000.00 0.00 0.18132 20000.00 18200.00 0.00 0.18308 20200.00 18200.00 0.00 0.18431 20000.00 18400.00 0.00 0.18599 20200.00 18400.00 0.00 0.18705 20000.00 18600.00 0.00 0.18885 20200.00 18600.00 0.00 0.18972 20000.00 18800.00 0.00 0.19207 20200.00 18800.00 0.00 0.19266 19800.00 19000.00 0.00 0.19344 20000.00 19000.00 0.00 0.19555 20200.00 19000.00 0.00 0.19580 19800.00 19200.00 0.00 0.19726 20000.00 19200.00 0.00 0.19897 19800.00 19400.00 0.00 0.20078 20000.00 19400.00 0.00 0.20198 19800.00 19600.00 0.00 0.20337 20000.00 19600.00 0.00 0.20400 19800.00 19800.00 0.00 0.20452 20000.00 19800.00 0.00 0.20464 19800.00 20000.00 10.00 0.20468 19800.00 20000.00 15.00 0.20475 19800.00 20000.00 20.00 0.20482 19800.00 20000.00 25.00 0.20488 19800.00 20000.00 30.00 0.20494 19800.00 20000.00 35.00 0.20499 19800.00 20000.00 40.00 0.20503 19800.00 20000.00 45.00 0.20505 19800.00 20000.00 50.00 0.20506 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 19790.00 20035.73 49.81 0.20507 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/193.ctf NX, NY, NZ: 360 360 1 MODE : real