CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/197.mrc Output diagnostic file name Micrographs/197.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/197.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2190.000 7650.000 Mean, RMS : 4435.383 220.6931 TITLE 1: emi2mrc: 197.emi Sun Jan 19 21:16:09 2014 TITLE 2: kV 300, Mag 78000x, Def 20350, Spot 4, Int 55.12%, Obj 88.07%, Dif 73.67% TITLE 3: X 117.300, Y 695.960, Z -35.048, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.01878 8200.00 8000.00 0.00 -0.01856 8400.00 8000.00 0.00 -0.01823 8600.00 8000.00 0.00 -0.01791 8800.00 8000.00 0.00 -0.01773 9000.00 8000.00 0.00 -0.01768 9600.00 8000.00 0.00 -0.01757 9800.00 8000.00 0.00 -0.01733 10000.00 8000.00 0.00 -0.01701 10200.00 8000.00 0.00 -0.01667 10400.00 8000.00 0.00 -0.01632 10600.00 8000.00 0.00 -0.01596 10800.00 8000.00 0.00 -0.01554 11000.00 8000.00 0.00 -0.01503 11200.00 8000.00 0.00 -0.01443 11400.00 8000.00 0.00 -0.01380 11600.00 8000.00 0.00 -0.01320 11800.00 8000.00 0.00 -0.01269 12000.00 8000.00 0.00 -0.01227 12200.00 8000.00 0.00 -0.01188 12400.00 8000.00 0.00 -0.01138 12600.00 8000.00 0.00 -0.01067 12800.00 8000.00 0.00 -0.00972 13000.00 8000.00 0.00 -0.00858 13200.00 8000.00 0.00 -0.00740 13400.00 8000.00 0.00 -0.00633 13600.00 8000.00 0.00 -0.00544 13800.00 8000.00 0.00 -0.00468 14000.00 8000.00 0.00 -0.00388 14200.00 8000.00 0.00 -0.00286 14400.00 8000.00 0.00 -0.00154 14600.00 8000.00 0.00 0.00005 14800.00 8000.00 0.00 0.00180 15000.00 8000.00 0.00 0.00357 15200.00 8000.00 0.00 0.00532 15400.00 8000.00 0.00 0.00708 15600.00 8000.00 0.00 0.00893 15800.00 8000.00 0.00 0.01090 16000.00 8000.00 0.00 0.01297 16200.00 8000.00 0.00 0.01507 16400.00 8000.00 0.00 0.01713 16600.00 8000.00 0.00 0.01914 16800.00 8000.00 0.00 0.02115 17000.00 8000.00 0.00 0.02322 17200.00 8000.00 0.00 0.02540 17400.00 8000.00 0.00 0.02770 17600.00 8000.00 0.00 0.03012 17800.00 8000.00 0.00 0.03264 18000.00 8000.00 0.00 0.03527 18200.00 8000.00 0.00 0.03803 18400.00 8000.00 0.00 0.04091 18600.00 8000.00 0.00 0.04387 18800.00 8000.00 0.00 0.04686 19000.00 8000.00 0.00 0.04985 19200.00 8000.00 0.00 0.05285 19400.00 8000.00 0.00 0.05593 19600.00 8000.00 0.00 0.05918 19800.00 8000.00 0.00 0.06264 20000.00 8000.00 0.00 0.06626 20200.00 8000.00 0.00 0.06984 20400.00 8000.00 0.00 0.07311 20600.00 8000.00 0.00 0.07580 20800.00 8000.00 0.00 0.07775 21000.00 8000.00 0.00 0.07891 21200.00 8000.00 0.00 0.07942 21400.00 8000.00 0.00 0.07951 21200.00 8200.00 0.00 0.07987 21400.00 8200.00 0.00 0.07999 21200.00 8400.00 0.00 0.08049 21400.00 8400.00 0.00 0.08065 21000.00 8600.00 0.00 0.08084 21200.00 8600.00 0.00 0.08142 21400.00 8600.00 0.00 0.08161 21000.00 8800.00 0.00 0.08202 21200.00 8800.00 0.00 0.08263 21400.00 8800.00 0.00 0.08284 21000.00 9000.00 0.00 0.08335 21200.00 9000.00 0.00 0.08399 21400.00 9000.00 0.00 0.08423 21000.00 9200.00 0.00 0.08480 21200.00 9200.00 0.00 0.08546 21400.00 9200.00 0.00 0.08572 21000.00 9400.00 0.00 0.08628 21200.00 9400.00 0.00 0.08695 21400.00 9400.00 0.00 0.08720 21000.00 9600.00 0.00 0.08773 21200.00 9600.00 0.00 0.08841 21400.00 9600.00 0.00 0.08865 21000.00 9800.00 0.00 0.08923 21200.00 9800.00 0.00 0.08990 21400.00 9800.00 0.00 0.09011 21000.00 10000.00 0.00 0.09076 21200.00 10000.00 0.00 0.09140 21400.00 10000.00 0.00 0.09159 21000.00 10200.00 0.00 0.09219 21200.00 10200.00 0.00 0.09280 21400.00 10200.00 0.00 0.09293 21000.00 10400.00 0.00 0.09346 21200.00 10400.00 0.00 0.09403 21400.00 10400.00 0.00 0.09410 21000.00 10600.00 0.00 0.09459 21200.00 10600.00 0.00 0.09511 21400.00 10600.00 0.00 0.09513 21000.00 10800.00 0.00 0.09563 21200.00 10800.00 0.00 0.09610 21000.00 11000.00 0.00 0.09671 21200.00 11000.00 0.00 0.09714 21000.00 11200.00 0.00 0.09797 21200.00 11200.00 0.00 0.09837 21000.00 11400.00 0.00 0.09934 21200.00 11400.00 0.00 0.09971 21000.00 11600.00 0.00 0.10074 21200.00 11600.00 0.00 0.10110 21000.00 11800.00 0.00 0.10217 21200.00 11800.00 0.00 0.10253 21000.00 12000.00 0.00 0.10363 21200.00 12000.00 0.00 0.10401 20800.00 12200.00 0.00 0.10410 21000.00 12200.00 0.00 0.10524 21200.00 12200.00 0.00 0.10564 20800.00 12400.00 0.00 0.10597 21000.00 12400.00 0.00 0.10714 21200.00 12400.00 0.00 0.10755 20800.00 12600.00 0.00 0.10810 21000.00 12600.00 0.00 0.10928 21200.00 12600.00 0.00 0.10969 20800.00 12800.00 0.00 0.11033 21000.00 12800.00 0.00 0.11151 21200.00 12800.00 0.00 0.11190 20800.00 13000.00 0.00 0.11255 21000.00 13000.00 0.00 0.11371 21200.00 13000.00 0.00 0.11406 20800.00 13200.00 0.00 0.11465 21000.00 13200.00 0.00 0.11578 21200.00 13200.00 0.00 0.11607 20800.00 13400.00 0.00 0.11664 21000.00 13400.00 0.00 0.11772 21200.00 13400.00 0.00 0.11795 20800.00 13600.00 0.00 0.11869 21000.00 13600.00 0.00 0.11972 21200.00 13600.00 0.00 0.11988 20800.00 13800.00 0.00 0.12091 21000.00 13800.00 0.00 0.12189 21200.00 13800.00 0.00 0.12199 20800.00 14000.00 0.00 0.12324 21000.00 14000.00 0.00 0.12417 21200.00 14000.00 0.00 0.12422 20800.00 14200.00 0.00 0.12565 21000.00 14200.00 0.00 0.12655 21200.00 14200.00 0.00 0.12656 20800.00 14400.00 0.00 0.12812 21000.00 14400.00 0.00 0.12901 20800.00 14600.00 0.00 0.13060 21000.00 14600.00 0.00 0.13148 20800.00 14800.00 0.00 0.13315 21000.00 14800.00 0.00 0.13403 20800.00 15000.00 0.00 0.13589 21000.00 15000.00 0.00 0.13675 20600.00 15200.00 0.00 0.13678 20800.00 15200.00 0.00 0.13872 21000.00 15200.00 0.00 0.13955 20600.00 15400.00 0.00 0.13958 20800.00 15400.00 0.00 0.14151 21000.00 15400.00 0.00 0.14231 20600.00 15600.00 0.00 0.14237 20800.00 15600.00 0.00 0.14427 21000.00 15600.00 0.00 0.14502 20600.00 15800.00 0.00 0.14509 20800.00 15800.00 0.00 0.14696 21000.00 15800.00 0.00 0.14764 20600.00 16000.00 0.00 0.14783 20800.00 16000.00 0.00 0.14965 21000.00 16000.00 0.00 0.15026 20600.00 16200.00 0.00 0.15080 20800.00 16200.00 0.00 0.15257 21000.00 16200.00 0.00 0.15310 20600.00 16400.00 0.00 0.15399 20800.00 16400.00 0.00 0.15571 21000.00 16400.00 0.00 0.15618 20600.00 16600.00 0.00 0.15718 20800.00 16600.00 0.00 0.15886 21000.00 16600.00 0.00 0.15927 20600.00 16800.00 0.00 0.16031 20800.00 16800.00 0.00 0.16195 21000.00 16800.00 0.00 0.16230 20600.00 17000.00 0.00 0.16332 20800.00 17000.00 0.00 0.16492 21000.00 17000.00 0.00 0.16519 20600.00 17200.00 0.00 0.16620 20800.00 17200.00 0.00 0.16774 21000.00 17200.00 0.00 0.16793 20600.00 17400.00 0.00 0.16920 20800.00 17400.00 0.00 0.17068 21000.00 17400.00 0.00 0.17077 20600.00 17600.00 0.00 0.17251 20800.00 17600.00 0.00 0.17391 20600.00 17800.00 0.00 0.17600 20800.00 17800.00 0.00 0.17730 20600.00 18000.00 0.00 0.17950 20800.00 18000.00 0.00 0.18070 20600.00 18200.00 0.00 0.18294 20800.00 18200.00 0.00 0.18403 20600.00 18400.00 0.00 0.18618 20800.00 18400.00 0.00 0.18717 20600.00 18600.00 0.00 0.18937 20800.00 18600.00 0.00 0.19023 20400.00 18800.00 0.00 0.19051 20600.00 18800.00 0.00 0.19276 20800.00 18800.00 0.00 0.19348 20400.00 19000.00 0.00 0.19414 20600.00 19000.00 0.00 0.19627 20800.00 19000.00 0.00 0.19682 20400.00 19200.00 0.00 0.19770 20600.00 19200.00 0.00 0.19968 20800.00 19200.00 0.00 0.20003 20400.00 19400.00 0.00 0.20109 20600.00 19400.00 0.00 0.20289 20800.00 19400.00 0.00 0.20299 20400.00 19600.00 0.00 0.20409 20600.00 19600.00 0.00 0.20567 20400.00 19800.00 0.00 0.20662 20600.00 19800.00 0.00 0.20797 20400.00 20000.00 0.00 0.20903 20600.00 20000.00 0.00 0.21009 20400.00 20200.00 0.00 0.21129 20600.00 20200.00 0.00 0.21197 20400.00 20400.00 0.00 0.21282 20600.00 20400.00 0.00 0.21304 20400.00 20600.00 0.00 0.21328 20400.00 20600.00 5.00 0.21332 20400.00 20600.00 10.00 0.21337 20400.00 20600.00 15.00 0.21341 20400.00 20600.00 20.00 0.21344 20400.00 20600.00 25.00 0.21346 20400.00 20600.00 30.00 0.21348 20400.00 20600.00 35.00 0.21349 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 20447.69 20588.54 32.69 0.21353 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/197.ctf NX, NY, NZ: 360 360 1 MODE : real