CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/206.mrc Output diagnostic file name Micrographs/206.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/206.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2274.000 7938.000 Mean, RMS : 4376.494 216.8275 TITLE 1: emi2mrc: 206.emi Sun Jan 19 21:23:01 2014 TITLE 2: kV 300, Mag 78000x, Def 20350, Spot 4, Int 55.12%, Obj 88.07%, Dif 73.67% TITLE 3: X 106.342, Y 678.660, Z -35.048, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 0.06166 8200.00 8000.00 0.00 0.06559 8400.00 8000.00 0.00 0.06957 8600.00 8000.00 0.00 0.07388 8800.00 8000.00 0.00 0.07887 9000.00 8000.00 0.00 0.08458 9200.00 8000.00 0.00 0.09097 9400.00 8000.00 0.00 0.09792 9600.00 8000.00 0.00 0.10508 9800.00 8000.00 0.00 0.11214 10000.00 8000.00 0.00 0.11922 10200.00 8000.00 0.00 0.12643 10400.00 8000.00 0.00 0.13385 10600.00 8000.00 0.00 0.14167 10800.00 8000.00 0.00 0.14983 11000.00 8000.00 0.00 0.15793 11200.00 8000.00 0.00 0.16554 11400.00 8000.00 0.00 0.17212 11600.00 8000.00 0.00 0.17695 11800.00 8000.00 0.00 0.17970 12000.00 8000.00 0.00 0.18051 11600.00 8200.00 0.00 0.18243 11800.00 8200.00 0.00 0.18514 12000.00 8200.00 0.00 0.18587 11600.00 8400.00 0.00 0.18797 11800.00 8400.00 0.00 0.19061 12000.00 8400.00 0.00 0.19124 11600.00 8600.00 0.00 0.19408 11800.00 8600.00 0.00 0.19662 12000.00 8600.00 0.00 0.19711 11600.00 8800.00 0.00 0.20085 11800.00 8800.00 0.00 0.20327 12000.00 8800.00 0.00 0.20359 11600.00 9000.00 0.00 0.20795 11800.00 9000.00 0.00 0.21022 12000.00 9000.00 0.00 0.21033 11400.00 9200.00 0.00 0.21074 11600.00 9200.00 0.00 0.21521 11800.00 9200.00 0.00 0.21731 11400.00 9400.00 0.00 0.21802 11600.00 9400.00 0.00 0.22236 11800.00 9400.00 0.00 0.22428 11400.00 9600.00 0.00 0.22511 11600.00 9600.00 0.00 0.22933 11800.00 9600.00 0.00 0.23105 11400.00 9800.00 0.00 0.23265 11600.00 9800.00 0.00 0.23672 11800.00 9800.00 0.00 0.23822 11400.00 10000.00 0.00 0.24107 11600.00 10000.00 0.00 0.24494 11800.00 10000.00 0.00 0.24617 11400.00 10200.00 0.00 0.25009 11600.00 10200.00 0.00 0.25371 11800.00 10200.00 0.00 0.25458 11400.00 10400.00 0.00 0.25943 11600.00 10400.00 0.00 0.26269 11800.00 10400.00 0.00 0.26310 11200.00 10600.00 0.00 0.26326 11400.00 10600.00 0.00 0.26861 11600.00 10600.00 0.00 0.27139 11200.00 10800.00 0.00 0.27174 11400.00 10800.00 0.00 0.27666 11600.00 10800.00 0.00 0.27887 11200.00 11000.00 0.00 0.27888 11400.00 11000.00 0.00 0.28334 11600.00 11000.00 0.00 0.28489 11200.00 11200.00 0.00 0.28501 11400.00 11200.00 0.00 0.28886 11600.00 11200.00 0.00 0.28953 11400.00 11400.00 0.00 0.29221 11400.00 11600.00 10.00 0.29226 11400.00 11600.00 15.00 0.29232 11400.00 11600.00 20.00 0.29237 11400.00 11600.00 25.00 0.29240 11400.00 11600.00 30.00 0.29241 11400.00 11600.00 35.00 0.29242 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 11414.56 11505.15 35.00 0.29283 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/206.ctf NX, NY, NZ: 360 360 1 MODE : real