CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/207.mrc Output diagnostic file name Micrographs/207.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/207.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2386.000 8404.000 Mean, RMS : 4432.062 218.7646 TITLE 1: emi2mrc: 207.emi Sun Jan 19 21:23:56 2014 TITLE 2: kV 300, Mag 78000x, Def 20350, Spot 4, Int 55.12%, Obj 88.07%, Dif 73.67% TITLE 3: X 108.097, Y 680.992, Z -35.048, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 0.05684 8200.00 8000.00 0.00 0.06063 8400.00 8000.00 0.00 0.06434 8600.00 8000.00 0.00 0.06811 8800.00 8000.00 0.00 0.07231 9000.00 8000.00 0.00 0.07715 9200.00 8000.00 0.00 0.08283 9400.00 8000.00 0.00 0.08952 9600.00 8000.00 0.00 0.09691 9800.00 8000.00 0.00 0.10462 10000.00 8000.00 0.00 0.11253 10200.00 8000.00 0.00 0.12057 10400.00 8000.00 0.00 0.12883 10600.00 8000.00 0.00 0.13768 10800.00 8000.00 0.00 0.14726 11000.00 8000.00 0.00 0.15726 11200.00 8000.00 0.00 0.16712 11400.00 8000.00 0.00 0.17591 11600.00 8000.00 0.00 0.18250 11800.00 8000.00 0.00 0.18620 12000.00 8000.00 0.00 0.18710 11600.00 8200.00 0.00 0.18821 11800.00 8200.00 0.00 0.19183 12000.00 8200.00 0.00 0.19256 11600.00 8400.00 0.00 0.19387 11800.00 8400.00 0.00 0.19738 12000.00 8400.00 0.00 0.19793 11600.00 8600.00 0.00 0.20010 11800.00 8600.00 0.00 0.20349 12000.00 8600.00 0.00 0.20383 11600.00 8800.00 0.00 0.20715 11800.00 8800.00 0.00 0.21040 12000.00 8800.00 0.00 0.21050 11600.00 9000.00 0.00 0.21483 11800.00 9000.00 0.00 0.21792 11600.00 9200.00 0.00 0.22307 11800.00 9200.00 0.00 0.22597 11600.00 9400.00 0.00 0.23155 11800.00 9400.00 0.00 0.23422 11600.00 9600.00 0.00 0.23997 11800.00 9600.00 0.00 0.24238 11400.00 9800.00 0.00 0.24267 11600.00 9800.00 0.00 0.24866 11800.00 9800.00 0.00 0.25075 11400.00 10000.00 0.00 0.25213 11600.00 10000.00 0.00 0.25789 11800.00 10000.00 0.00 0.25959 11400.00 10200.00 0.00 0.26199 11600.00 10200.00 0.00 0.26747 11800.00 10200.00 0.00 0.26870 11400.00 10400.00 0.00 0.27240 11600.00 10400.00 0.00 0.27750 11800.00 10400.00 0.00 0.27814 11400.00 10600.00 0.00 0.28342 11600.00 10600.00 0.00 0.28797 11400.00 10800.00 0.00 0.29437 11600.00 10800.00 0.00 0.29819 11400.00 11000.00 0.00 0.30474 11600.00 11000.00 0.00 0.30763 11400.00 11200.00 0.00 0.31400 11600.00 11200.00 0.00 0.31559 11400.00 11400.00 0.00 0.32005 11400.00 11600.00 0.00 0.32068 11400.00 11600.00 5.00 0.32090 11400.00 11600.00 10.00 0.32109 11400.00 11600.00 15.00 0.32127 11400.00 11600.00 20.00 0.32142 11400.00 11600.00 25.00 0.32153 11400.00 11600.00 30.00 0.32161 11400.00 11600.00 35.00 0.32164 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 11415.80 11550.94 37.05 0.32180 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/207.ctf NX, NY, NZ: 360 360 1 MODE : real