CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/209.mrc Output diagnostic file name Micrographs/209.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/209.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2492.000 7988.000 Mean, RMS : 4433.503 219.6402 TITLE 1: emi2mrc: 209.emi Sun Jan 19 21:25:13 2014 TITLE 2: kV 300, Mag 78000x, Def 20350, Spot 4, Int 55.12%, Obj 88.07%, Dif 73.67% TITLE 3: X 111.573, Y 684.957, Z -35.048, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 0.04978 8200.00 8000.00 0.00 0.05330 8400.00 8000.00 0.00 0.05700 8600.00 8000.00 0.00 0.06109 8800.00 8000.00 0.00 0.06578 9000.00 8000.00 0.00 0.07096 9200.00 8000.00 0.00 0.07657 9400.00 8000.00 0.00 0.08271 9600.00 8000.00 0.00 0.08934 9800.00 8000.00 0.00 0.09643 10000.00 8000.00 0.00 0.10406 10200.00 8000.00 0.00 0.11212 10400.00 8000.00 0.00 0.12043 10600.00 8000.00 0.00 0.12909 10800.00 8000.00 0.00 0.13824 11000.00 8000.00 0.00 0.14781 11200.00 8000.00 0.00 0.15765 11400.00 8000.00 0.00 0.16708 11600.00 8000.00 0.00 0.17494 11800.00 8000.00 0.00 0.18021 12000.00 8000.00 0.00 0.18254 11800.00 8200.00 0.00 0.18559 12000.00 8200.00 0.00 0.18783 11800.00 8400.00 0.00 0.19127 12000.00 8400.00 0.00 0.19338 11800.00 8600.00 0.00 0.19769 12000.00 8600.00 0.00 0.19965 11600.00 8800.00 0.00 0.19971 11800.00 8800.00 0.00 0.20478 12000.00 8800.00 0.00 0.20655 11600.00 9000.00 0.00 0.20715 11800.00 9000.00 0.00 0.21211 12000.00 9000.00 0.00 0.21366 11600.00 9200.00 0.00 0.21472 11800.00 9200.00 0.00 0.21954 12000.00 9200.00 0.00 0.22086 11600.00 9400.00 0.00 0.22231 11800.00 9400.00 0.00 0.22699 12000.00 9400.00 0.00 0.22804 11600.00 9600.00 0.00 0.22996 11800.00 9600.00 0.00 0.23447 12000.00 9600.00 0.00 0.23526 11600.00 9800.00 0.00 0.23821 11800.00 9800.00 0.00 0.24253 12000.00 9800.00 0.00 0.24299 11600.00 10000.00 0.00 0.24723 11800.00 10000.00 0.00 0.25132 12000.00 10000.00 0.00 0.25139 11600.00 10200.00 0.00 0.25651 11800.00 10200.00 0.00 0.26029 11600.00 10400.00 0.00 0.26574 11800.00 10400.00 0.00 0.26913 11600.00 10600.00 0.00 0.27488 11800.00 10600.00 0.00 0.27775 11600.00 10800.00 0.00 0.28370 11800.00 10800.00 0.00 0.28590 11400.00 11000.00 0.00 0.28626 11600.00 11000.00 0.00 0.29252 11800.00 11000.00 0.00 0.29386 11400.00 11200.00 0.00 0.29611 11600.00 11200.00 0.00 0.30148 11800.00 11200.00 0.00 0.30165 11400.00 11400.00 0.00 0.30487 11600.00 11400.00 0.00 0.30891 11400.00 11600.00 0.00 0.31012 11600.00 11600.00 0.00 0.31255 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 11542.40 11697.91 25.91 0.31311 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/209.ctf NX, NY, NZ: 360 360 1 MODE : real