CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/210.mrc Output diagnostic file name Micrographs/210.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/210.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2254.000 7908.000 Mean, RMS : 4433.783 217.5428 TITLE 1: emi2mrc: 210.emi Sun Jan 19 21:27:19 2014 TITLE 2: kV 300, Mag 78000x, Def 20350, Spot 4, Int 55.12%, Obj 88.07%, Dif 73.67% TITLE 3: X 114.652, Y 688.898, Z -35.048, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 0.03068 8200.00 8000.00 0.00 0.03245 8400.00 8000.00 0.00 0.03435 8600.00 8000.00 0.00 0.03651 8800.00 8000.00 0.00 0.03902 9000.00 8000.00 0.00 0.04172 9200.00 8000.00 0.00 0.04448 9400.00 8000.00 0.00 0.04731 9600.00 8000.00 0.00 0.05028 9800.00 8000.00 0.00 0.05355 10000.00 8000.00 0.00 0.05737 10200.00 8000.00 0.00 0.06179 10400.00 8000.00 0.00 0.06671 10600.00 8000.00 0.00 0.07205 10800.00 8000.00 0.00 0.07769 11000.00 8000.00 0.00 0.08352 11200.00 8000.00 0.00 0.08957 11400.00 8000.00 0.00 0.09586 11600.00 8000.00 0.00 0.10230 11800.00 8000.00 0.00 0.10890 12000.00 8000.00 0.00 0.11581 12200.00 8000.00 0.00 0.12312 12400.00 8000.00 0.00 0.13084 12600.00 8000.00 0.00 0.13873 12800.00 8000.00 0.00 0.14605 13000.00 8000.00 0.00 0.15191 13200.00 8000.00 0.00 0.15567 13400.00 8000.00 0.00 0.15717 13200.00 8200.00 0.00 0.15910 13400.00 8200.00 0.00 0.16053 13200.00 8400.00 0.00 0.16258 13400.00 8400.00 0.00 0.16395 13200.00 8600.00 0.00 0.16652 13400.00 8600.00 0.00 0.16782 13200.00 8800.00 0.00 0.17092 13400.00 8800.00 0.00 0.17215 13200.00 9000.00 0.00 0.17557 13400.00 9000.00 0.00 0.17671 13000.00 9200.00 0.00 0.17683 13200.00 9200.00 0.00 0.18043 13400.00 9200.00 0.00 0.18149 13000.00 9400.00 0.00 0.18185 13200.00 9400.00 0.00 0.18541 13400.00 9400.00 0.00 0.18638 13000.00 9600.00 0.00 0.18697 13200.00 9600.00 0.00 0.19047 13400.00 9600.00 0.00 0.19132 13000.00 9800.00 0.00 0.19249 13200.00 9800.00 0.00 0.19591 13400.00 9800.00 0.00 0.19661 13000.00 10000.00 0.00 0.19852 13200.00 10000.00 0.00 0.20183 13400.00 10000.00 0.00 0.20236 13000.00 10200.00 0.00 0.20471 13200.00 10200.00 0.00 0.20790 13400.00 10200.00 0.00 0.20822 13000.00 10400.00 0.00 0.21087 13200.00 10400.00 0.00 0.21392 13400.00 10400.00 0.00 0.21401 13000.00 10600.00 0.00 0.21698 13200.00 10600.00 0.00 0.21986 13000.00 10800.00 0.00 0.22299 13200.00 10800.00 0.00 0.22570 13000.00 11000.00 0.00 0.22928 13200.00 11000.00 0.00 0.23179 13000.00 11200.00 0.00 0.23625 13200.00 11200.00 0.00 0.23851 13000.00 11400.00 0.00 0.24356 13200.00 11400.00 0.00 0.24551 12800.00 11600.00 0.00 0.24563 13000.00 11600.00 0.00 0.25073 13200.00 11600.00 0.00 0.25231 12800.00 11800.00 0.00 0.25279 13000.00 11800.00 0.00 0.25763 13200.00 11800.00 0.00 0.25877 12800.00 12000.00 0.00 0.25950 13000.00 12000.00 0.00 0.26404 13200.00 12000.00 0.00 0.26467 12800.00 12200.00 0.00 0.26595 13000.00 12200.00 0.00 0.27010 13200.00 12200.00 0.00 0.27014 12800.00 12400.00 0.00 0.27280 13000.00 12400.00 0.00 0.27638 12800.00 12600.00 0.00 0.27963 13000.00 12600.00 0.00 0.28236 12800.00 12800.00 0.00 0.28479 13000.00 12800.00 0.00 0.28643 12800.00 13000.00 0.00 0.28717 13000.00 13000.00 0.00 0.28760 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 12910.32 13005.15 14.45 0.28801 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/210.ctf NX, NY, NZ: 360 360 1 MODE : real