CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/216.mrc Output diagnostic file name Micrographs/216.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/216.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2482.000 8216.000 Mean, RMS : 4432.703 220.9335 TITLE 1: emi2mrc: 216.emi Sun Jan 19 21:31:45 2014 TITLE 2: kV 300, Mag 78000x, Def 20350, Spot 4, Int 55.12%, Obj 88.07%, Dif 73.67% TITLE 3: X 124.447, Y 701.030, Z -35.048, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 118 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.02125 10600.00 8000.00 0.00 -0.02109 10800.00 8000.00 0.00 -0.02073 11000.00 8000.00 0.00 -0.02023 11200.00 8000.00 0.00 -0.01967 11400.00 8000.00 0.00 -0.01904 11600.00 8000.00 0.00 -0.01833 11800.00 8000.00 0.00 -0.01755 12000.00 8000.00 0.00 -0.01674 12200.00 8000.00 0.00 -0.01598 12400.00 8000.00 0.00 -0.01533 12600.00 8000.00 0.00 -0.01483 12800.00 8000.00 0.00 -0.01440 13000.00 8000.00 0.00 -0.01394 13200.00 8000.00 0.00 -0.01335 13400.00 8000.00 0.00 -0.01258 13600.00 8000.00 0.00 -0.01166 13800.00 8000.00 0.00 -0.01062 14000.00 8000.00 0.00 -0.00953 14200.00 8000.00 0.00 -0.00842 14400.00 8000.00 0.00 -0.00728 14600.00 8000.00 0.00 -0.00610 14800.00 8000.00 0.00 -0.00486 15000.00 8000.00 0.00 -0.00355 15200.00 8000.00 0.00 -0.00213 15400.00 8000.00 0.00 -0.00060 15600.00 8000.00 0.00 0.00102 15800.00 8000.00 0.00 0.00273 16000.00 8000.00 0.00 0.00450 16200.00 8000.00 0.00 0.00634 16400.00 8000.00 0.00 0.00827 16600.00 8000.00 0.00 0.01034 16800.00 8000.00 0.00 0.01251 17000.00 8000.00 0.00 0.01472 17200.00 8000.00 0.00 0.01688 17400.00 8000.00 0.00 0.01895 17600.00 8000.00 0.00 0.02095 17800.00 8000.00 0.00 0.02298 18000.00 8000.00 0.00 0.02510 18200.00 8000.00 0.00 0.02734 18400.00 8000.00 0.00 0.02965 18600.00 8000.00 0.00 0.03192 18800.00 8000.00 0.00 0.03413 19000.00 8000.00 0.00 0.03634 19200.00 8000.00 0.00 0.03865 19400.00 8000.00 0.00 0.04120 19600.00 8000.00 0.00 0.04399 19800.00 8000.00 0.00 0.04694 20000.00 8000.00 0.00 0.04992 20200.00 8000.00 0.00 0.05285 20400.00 8000.00 0.00 0.05571 20600.00 8000.00 0.00 0.05860 20800.00 8000.00 0.00 0.06159 21000.00 8000.00 0.00 0.06462 21200.00 8000.00 0.00 0.06750 21400.00 8000.00 0.00 0.06998 21600.00 8000.00 0.00 0.07182 21800.00 8000.00 0.00 0.07292 22000.00 8000.00 0.00 0.07343 22200.00 8000.00 0.00 0.07355 21800.00 8200.00 0.00 0.07384 22000.00 8200.00 0.00 0.07432 22200.00 8200.00 0.00 0.07442 21800.00 8400.00 0.00 0.07465 22000.00 8400.00 0.00 0.07510 22200.00 8400.00 0.00 0.07517 21800.00 8600.00 0.00 0.07550 22000.00 8600.00 0.00 0.07591 22200.00 8600.00 0.00 0.07594 21800.00 8800.00 0.00 0.07640 22000.00 8800.00 0.00 0.07678 21800.00 9000.00 0.00 0.07732 22000.00 9000.00 0.00 0.07768 21800.00 9200.00 0.00 0.07827 22000.00 9200.00 0.00 0.07861 21800.00 9400.00 0.00 0.07923 22000.00 9400.00 0.00 0.07956 21800.00 9600.00 0.00 0.08020 22000.00 9600.00 0.00 0.08052 21800.00 9800.00 0.00 0.08128 22000.00 9800.00 0.00 0.08160 21800.00 10000.00 0.00 0.08247 22000.00 10000.00 0.00 0.08277 21800.00 10200.00 0.00 0.08362 22000.00 10200.00 0.00 0.08392 21800.00 10400.00 0.00 0.08468 22000.00 10400.00 0.00 0.08498 21800.00 10600.00 0.00 0.08565 22000.00 10600.00 0.00 0.08593 21800.00 10800.00 0.00 0.08654 22000.00 10800.00 0.00 0.08681 21800.00 11000.00 0.00 0.08752 22000.00 11000.00 0.00 0.08778 21600.00 11200.00 0.00 0.08792 21800.00 11200.00 0.00 0.08873 22000.00 11200.00 0.00 0.08898 21600.00 11400.00 0.00 0.08936 21800.00 11400.00 0.00 0.09016 22000.00 11400.00 0.00 0.09040 21600.00 11600.00 0.00 0.09091 21800.00 11600.00 0.00 0.09170 22000.00 11600.00 0.00 0.09195 21600.00 11800.00 0.00 0.09252 21800.00 11800.00 0.00 0.09332 22000.00 11800.00 0.00 0.09357 21600.00 12000.00 0.00 0.09412 21800.00 12000.00 0.00 0.09493 22000.00 12000.00 0.00 0.09520 21600.00 12200.00 0.00 0.09574 21800.00 12200.00 0.00 0.09656 22000.00 12200.00 0.00 0.09685 21600.00 12400.00 0.00 0.09749 21800.00 12400.00 0.00 0.09832 22000.00 12400.00 0.00 0.09863 22200.00 12400.00 0.00 0.09864 21600.00 12600.00 0.00 0.09936 21800.00 12600.00 0.00 0.10020 22000.00 12600.00 0.00 0.10052 22200.00 12600.00 0.00 0.10054 21600.00 12800.00 0.00 0.10122 21800.00 12800.00 0.00 0.10207 22000.00 12800.00 0.00 0.10239 22200.00 12800.00 0.00 0.10239 21600.00 13000.00 0.00 0.10302 21800.00 13000.00 0.00 0.10385 22000.00 13000.00 0.00 0.10416 21600.00 13200.00 0.00 0.10469 21800.00 13200.00 0.00 0.10551 22000.00 13200.00 0.00 0.10581 21600.00 13400.00 0.00 0.10628 21800.00 13400.00 0.00 0.10710 22000.00 13400.00 0.00 0.10738 21600.00 13600.00 0.00 0.10801 21800.00 13600.00 0.00 0.10882 22000.00 13600.00 0.00 0.10910 21600.00 13800.00 0.00 0.10998 21800.00 13800.00 0.00 0.11080 22000.00 13800.00 0.00 0.11108 21600.00 14000.00 0.00 0.11215 21800.00 14000.00 0.00 0.11297 22000.00 14000.00 0.00 0.11323 21600.00 14200.00 0.00 0.11440 21800.00 14200.00 0.00 0.11521 22000.00 14200.00 0.00 0.11546 21600.00 14400.00 0.00 0.11663 21800.00 14400.00 0.00 0.11741 22000.00 14400.00 0.00 0.11763 21600.00 14600.00 0.00 0.11870 21800.00 14600.00 0.00 0.11947 22000.00 14600.00 0.00 0.11965 21600.00 14800.00 0.00 0.12074 21800.00 14800.00 0.00 0.12148 22000.00 14800.00 0.00 0.12163 21600.00 15000.00 0.00 0.12291 21800.00 15000.00 0.00 0.12363 22000.00 15000.00 0.00 0.12374 21600.00 15200.00 0.00 0.12520 21800.00 15200.00 0.00 0.12589 22000.00 15200.00 0.00 0.12595 21400.00 15400.00 0.00 0.12608 21600.00 15400.00 0.00 0.12757 21800.00 15400.00 0.00 0.12821 22000.00 15400.00 0.00 0.12822 21400.00 15600.00 0.00 0.12851 21600.00 15600.00 0.00 0.12996 21800.00 15600.00 0.00 0.13056 21400.00 15800.00 0.00 0.13090 21600.00 15800.00 0.00 0.13231 21800.00 15800.00 0.00 0.13285 21400.00 16000.00 0.00 0.13325 21600.00 16000.00 0.00 0.13462 21800.00 16000.00 0.00 0.13511 21400.00 16200.00 0.00 0.13576 21600.00 16200.00 0.00 0.13708 21800.00 16200.00 0.00 0.13752 21400.00 16400.00 0.00 0.13844 21600.00 16400.00 0.00 0.13971 21800.00 16400.00 0.00 0.14008 21400.00 16600.00 0.00 0.14116 21600.00 16600.00 0.00 0.14237 21800.00 16600.00 0.00 0.14270 21400.00 16800.00 0.00 0.14388 21600.00 16800.00 0.00 0.14505 21800.00 16800.00 0.00 0.14533 21400.00 17000.00 0.00 0.14653 21600.00 17000.00 0.00 0.14765 21800.00 17000.00 0.00 0.14790 21400.00 17200.00 0.00 0.14904 21600.00 17200.00 0.00 0.15013 21800.00 17200.00 0.00 0.15034 21400.00 17400.00 0.00 0.15159 21600.00 17400.00 0.00 0.15265 21800.00 17400.00 0.00 0.15283 21400.00 17600.00 0.00 0.15435 21600.00 17600.00 0.00 0.15538 21800.00 17600.00 0.00 0.15552 21400.00 17800.00 0.00 0.15722 21600.00 17800.00 0.00 0.15821 21800.00 17800.00 0.00 0.15830 21400.00 18000.00 0.00 0.16011 21600.00 18000.00 0.00 0.16107 21800.00 18000.00 0.00 0.16110 21400.00 18200.00 0.00 0.16301 21600.00 18200.00 0.00 0.16393 21400.00 18400.00 0.00 0.16583 21600.00 18400.00 0.00 0.16671 21200.00 18600.00 0.00 0.16679 21400.00 18600.00 0.00 0.16866 21600.00 18600.00 0.00 0.16949 21200.00 18800.00 0.00 0.16988 21400.00 18800.00 0.00 0.17170 21600.00 18800.00 0.00 0.17246 21200.00 19000.00 0.00 0.17311 21400.00 19000.00 0.00 0.17487 21600.00 19000.00 0.00 0.17554 21200.00 19200.00 0.00 0.17628 21400.00 19200.00 0.00 0.17796 21600.00 19200.00 0.00 0.17852 21200.00 19400.00 0.00 0.17931 21400.00 19400.00 0.00 0.18091 21600.00 19400.00 0.00 0.18135 21200.00 19600.00 0.00 0.18211 21400.00 19600.00 0.00 0.18362 21600.00 19600.00 0.00 0.18395 21200.00 19800.00 0.00 0.18476 21400.00 19800.00 0.00 0.18618 21600.00 19800.00 0.00 0.18637 21200.00 20000.00 0.00 0.18765 21400.00 20000.00 0.00 0.18894 21200.00 20200.00 0.00 0.19089 21400.00 20200.00 0.00 0.19196 21200.00 20400.00 0.00 0.19416 21400.00 20400.00 0.00 0.19494 21000.00 20600.00 0.00 0.19531 21200.00 20600.00 0.00 0.19720 21400.00 20600.00 0.00 0.19761 21000.00 20800.00 0.00 0.19815 21200.00 20800.00 0.00 0.19960 21000.00 21000.00 0.00 0.19989 21200.00 21000.00 0.00 0.20087 21200.00 21200.00 0.00 0.20113 21000.00 21400.00 30.00 0.20116 21000.00 21400.00 35.00 0.20118 21000.00 21200.00 40.00 0.20119 21000.00 21200.00 45.00 0.20123 21000.00 21200.00 50.00 0.20124 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 21039.95 21275.48 47.18 0.20138 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/216.ctf NX, NY, NZ: 360 360 1 MODE : real