CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/225.mrc Output diagnostic file name Micrographs/225.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/225.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2290.000 7870.000 Mean, RMS : 4419.382 225.5513 TITLE 1: emi2mrc: 225.emi Sun Jan 19 21:44:46 2014 TITLE 2: kV 300, Mag 78000x, Def 20670, Spot 4, Int 55.12%, Obj 88.08%, Dif 73.67% TITLE 3: X 111.322, Y 680.611, Z -36.397, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.01267 8400.00 8000.00 0.00 -0.01265 8600.00 8000.00 0.00 -0.01258 8800.00 8000.00 0.00 -0.01247 9000.00 8000.00 0.00 -0.01229 9200.00 8000.00 0.00 -0.01203 9400.00 8000.00 0.00 -0.01174 9600.00 8000.00 0.00 -0.01146 9800.00 8000.00 0.00 -0.01119 10000.00 8000.00 0.00 -0.01088 10200.00 8000.00 0.00 -0.01049 10400.00 8000.00 0.00 -0.00999 10600.00 8000.00 0.00 -0.00946 10800.00 8000.00 0.00 -0.00899 11000.00 8000.00 0.00 -0.00865 11200.00 8000.00 0.00 -0.00839 11400.00 8000.00 0.00 -0.00806 11600.00 8000.00 0.00 -0.00750 11800.00 8000.00 0.00 -0.00666 12000.00 8000.00 0.00 -0.00560 12200.00 8000.00 0.00 -0.00448 12400.00 8000.00 0.00 -0.00344 12600.00 8000.00 0.00 -0.00252 12800.00 8000.00 0.00 -0.00162 13000.00 8000.00 0.00 -0.00061 13200.00 8000.00 0.00 0.00059 13400.00 8000.00 0.00 0.00195 13600.00 8000.00 0.00 0.00338 13800.00 8000.00 0.00 0.00480 14000.00 8000.00 0.00 0.00619 14200.00 8000.00 0.00 0.00759 14400.00 8000.00 0.00 0.00908 14600.00 8000.00 0.00 0.01068 14800.00 8000.00 0.00 0.01238 15000.00 8000.00 0.00 0.01417 15200.00 8000.00 0.00 0.01602 15400.00 8000.00 0.00 0.01795 15600.00 8000.00 0.00 0.01996 15800.00 8000.00 0.00 0.02204 16000.00 8000.00 0.00 0.02418 16200.00 8000.00 0.00 0.02637 16400.00 8000.00 0.00 0.02863 16600.00 8000.00 0.00 0.03098 16800.00 8000.00 0.00 0.03339 17000.00 8000.00 0.00 0.03582 17200.00 8000.00 0.00 0.03819 17400.00 8000.00 0.00 0.04049 17600.00 8000.00 0.00 0.04279 17800.00 8000.00 0.00 0.04522 18000.00 8000.00 0.00 0.04787 18200.00 8000.00 0.00 0.05077 18400.00 8000.00 0.00 0.05382 18600.00 8000.00 0.00 0.05692 18800.00 8000.00 0.00 0.06006 19000.00 8000.00 0.00 0.06332 19200.00 8000.00 0.00 0.06681 19400.00 8000.00 0.00 0.07058 19600.00 8000.00 0.00 0.07450 19800.00 8000.00 0.00 0.07824 20000.00 8000.00 0.00 0.08144 20200.00 8000.00 0.00 0.08379 20400.00 8000.00 0.00 0.08519 20600.00 8000.00 0.00 0.08579 20800.00 8000.00 0.00 0.08589 20400.00 8200.00 0.00 0.08626 20600.00 8200.00 0.00 0.08685 20800.00 8200.00 0.00 0.08692 20400.00 8400.00 0.00 0.08720 20600.00 8400.00 0.00 0.08776 20800.00 8400.00 0.00 0.08781 20400.00 8600.00 0.00 0.08819 20600.00 8600.00 0.00 0.08873 20800.00 8600.00 0.00 0.08875 20400.00 8800.00 0.00 0.08928 20600.00 8800.00 0.00 0.08980 20400.00 9000.00 0.00 0.09043 20600.00 9000.00 0.00 0.09093 20400.00 9200.00 0.00 0.09164 20600.00 9200.00 0.00 0.09214 20400.00 9400.00 0.00 0.09285 20600.00 9400.00 0.00 0.09334 20400.00 9600.00 0.00 0.09402 20600.00 9600.00 0.00 0.09451 20400.00 9800.00 0.00 0.09527 20600.00 9800.00 0.00 0.09575 20400.00 10000.00 0.00 0.09665 20600.00 10000.00 0.00 0.09714 20800.00 10000.00 0.00 0.09714 20400.00 10200.00 0.00 0.09810 20600.00 10200.00 0.00 0.09859 20800.00 10200.00 0.00 0.09861 20400.00 10400.00 0.00 0.09958 20600.00 10400.00 0.00 0.10009 20800.00 10400.00 0.00 0.10013 20400.00 10600.00 0.00 0.10109 20600.00 10600.00 0.00 0.10162 20800.00 10600.00 0.00 0.10168 20400.00 10800.00 0.00 0.10259 20600.00 10800.00 0.00 0.10315 20800.00 10800.00 0.00 0.10323 20400.00 11000.00 0.00 0.10416 20600.00 11000.00 0.00 0.10475 20800.00 11000.00 0.00 0.10484 20400.00 11200.00 0.00 0.10590 20600.00 11200.00 0.00 0.10650 20800.00 11200.00 0.00 0.10660 20400.00 11400.00 0.00 0.10772 20600.00 11400.00 0.00 0.10832 20800.00 11400.00 0.00 0.10841 20400.00 11600.00 0.00 0.10952 20600.00 11600.00 0.00 0.11011 20800.00 11600.00 0.00 0.11017 20400.00 11800.00 0.00 0.11127 20600.00 11800.00 0.00 0.11182 20800.00 11800.00 0.00 0.11184 20400.00 12000.00 0.00 0.11295 20600.00 12000.00 0.00 0.11346 20400.00 12200.00 0.00 0.11466 20600.00 12200.00 0.00 0.11513 20200.00 12400.00 0.00 0.11524 20400.00 12400.00 0.00 0.11659 20600.00 12400.00 0.00 0.11703 20200.00 12600.00 0.00 0.11741 20400.00 12600.00 0.00 0.11874 20600.00 12600.00 0.00 0.11916 20200.00 12800.00 0.00 0.11965 20400.00 12800.00 0.00 0.12097 20600.00 12800.00 0.00 0.12137 20200.00 13000.00 0.00 0.12185 20400.00 13000.00 0.00 0.12317 20600.00 13000.00 0.00 0.12356 20200.00 13200.00 0.00 0.12392 20400.00 13200.00 0.00 0.12523 20600.00 13200.00 0.00 0.12561 20200.00 13400.00 0.00 0.12587 20400.00 13400.00 0.00 0.12718 20600.00 13400.00 0.00 0.12754 20200.00 13600.00 0.00 0.12793 20400.00 13600.00 0.00 0.12925 20600.00 13600.00 0.00 0.12960 20200.00 13800.00 0.00 0.13031 20400.00 13800.00 0.00 0.13162 20600.00 13800.00 0.00 0.13193 20200.00 14000.00 0.00 0.13293 20400.00 14000.00 0.00 0.13421 20600.00 14000.00 0.00 0.13447 20200.00 14200.00 0.00 0.13572 20400.00 14200.00 0.00 0.13695 20600.00 14200.00 0.00 0.13715 20200.00 14400.00 0.00 0.13858 20400.00 14400.00 0.00 0.13976 20600.00 14400.00 0.00 0.13990 20200.00 14600.00 0.00 0.14141 20400.00 14600.00 0.00 0.14255 20600.00 14600.00 0.00 0.14264 20200.00 14800.00 0.00 0.14433 20400.00 14800.00 0.00 0.14543 20600.00 14800.00 0.00 0.14547 20200.00 15000.00 0.00 0.14752 20400.00 15000.00 0.00 0.14857 20200.00 15200.00 0.00 0.15088 20400.00 15200.00 0.00 0.15190 20000.00 15400.00 0.00 0.15192 20200.00 15400.00 0.00 0.15428 20400.00 15400.00 0.00 0.15526 20000.00 15600.00 0.00 0.15529 20200.00 15600.00 0.00 0.15763 20400.00 15600.00 0.00 0.15857 20200.00 15800.00 0.00 0.16082 20400.00 15800.00 0.00 0.16173 20200.00 16000.00 0.00 0.16392 20400.00 16000.00 0.00 0.16479 20000.00 16200.00 0.00 0.16496 20200.00 16200.00 0.00 0.16726 20400.00 16200.00 0.00 0.16807 20000.00 16400.00 0.00 0.16867 20200.00 16400.00 0.00 0.17093 20400.00 16400.00 0.00 0.17167 20000.00 16600.00 0.00 0.17255 20200.00 16600.00 0.00 0.17475 20400.00 16600.00 0.00 0.17539 20000.00 16800.00 0.00 0.17644 20200.00 16800.00 0.00 0.17857 20400.00 16800.00 0.00 0.17909 20000.00 17000.00 0.00 0.18017 20200.00 17000.00 0.00 0.18221 20400.00 17000.00 0.00 0.18262 20000.00 17200.00 0.00 0.18366 20200.00 17200.00 0.00 0.18560 20400.00 17200.00 0.00 0.18589 20000.00 17400.00 0.00 0.18722 20200.00 17400.00 0.00 0.18907 20400.00 17400.00 0.00 0.18922 20000.00 17600.00 0.00 0.19123 20200.00 17600.00 0.00 0.19297 20400.00 17600.00 0.00 0.19298 20000.00 17800.00 0.00 0.19567 20200.00 17800.00 0.00 0.19729 20000.00 18000.00 0.00 0.20039 20200.00 18000.00 0.00 0.20187 20000.00 18200.00 0.00 0.20517 20200.00 18200.00 0.00 0.20652 20000.00 18400.00 0.00 0.20974 20200.00 18400.00 0.00 0.21093 19800.00 18600.00 0.00 0.21107 20000.00 18600.00 0.00 0.21421 20200.00 18600.00 0.00 0.21522 19800.00 18800.00 0.00 0.21609 20000.00 18800.00 0.00 0.21906 20200.00 18800.00 0.00 0.21980 19800.00 19000.00 0.00 0.22172 20000.00 19000.00 0.00 0.22439 20200.00 19000.00 0.00 0.22474 19800.00 19200.00 0.00 0.22775 20000.00 19200.00 0.00 0.22995 19800.00 19400.00 0.00 0.23378 20000.00 19400.00 0.00 0.23533 19800.00 19600.00 0.00 0.23878 20000.00 19600.00 0.00 0.23952 19800.00 19800.00 0.00 0.24160 19800.00 20000.00 0.00 0.24210 19800.00 20000.00 5.00 0.24224 19800.00 20000.00 10.00 0.24236 19800.00 20000.00 15.00 0.24248 19800.00 20000.00 20.00 0.24257 19800.00 20000.00 25.00 0.24264 19800.00 20000.00 30.00 0.24269 19800.00 20000.00 35.00 0.24270 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 19782.70 20019.28 35.00 0.24272 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/225.ctf NX, NY, NZ: 360 360 1 MODE : real