CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/229.mrc Output diagnostic file name Micrographs/229.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/229.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2032.000 7436.000 Mean, RMS : 4312.712 216.1704 TITLE 1: emi2mrc: 229.emi Sun Jan 19 21:47:42 2014 TITLE 2: kV 300, Mag 78000x, Def 20670, Spot 4, Int 55.12%, Obj 88.08%, Dif 73.67% TITLE 3: X 113.797, Y 679.495, Z -36.397, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.01052 8200.00 8000.00 0.00 -0.01009 8400.00 8000.00 0.00 -0.00960 8600.00 8000.00 0.00 -0.00911 8800.00 8000.00 0.00 -0.00865 9000.00 8000.00 0.00 -0.00818 9200.00 8000.00 0.00 -0.00768 9400.00 8000.00 0.00 -0.00714 9600.00 8000.00 0.00 -0.00658 9800.00 8000.00 0.00 -0.00599 10000.00 8000.00 0.00 -0.00539 10200.00 8000.00 0.00 -0.00474 10400.00 8000.00 0.00 -0.00400 10600.00 8000.00 0.00 -0.00318 10800.00 8000.00 0.00 -0.00229 11000.00 8000.00 0.00 -0.00135 11200.00 8000.00 0.00 -0.00034 11400.00 8000.00 0.00 0.00076 11600.00 8000.00 0.00 0.00196 11800.00 8000.00 0.00 0.00323 12000.00 8000.00 0.00 0.00453 12200.00 8000.00 0.00 0.00582 12400.00 8000.00 0.00 0.00712 12600.00 8000.00 0.00 0.00852 12800.00 8000.00 0.00 0.01010 13000.00 8000.00 0.00 0.01189 13200.00 8000.00 0.00 0.01384 13400.00 8000.00 0.00 0.01582 13600.00 8000.00 0.00 0.01776 13800.00 8000.00 0.00 0.01966 14000.00 8000.00 0.00 0.02161 14200.00 8000.00 0.00 0.02371 14400.00 8000.00 0.00 0.02601 14600.00 8000.00 0.00 0.02847 14800.00 8000.00 0.00 0.03098 15000.00 8000.00 0.00 0.03349 15200.00 8000.00 0.00 0.03599 15400.00 8000.00 0.00 0.03852 15600.00 8000.00 0.00 0.04116 15800.00 8000.00 0.00 0.04392 16000.00 8000.00 0.00 0.04674 16200.00 8000.00 0.00 0.04961 16400.00 8000.00 0.00 0.05253 16600.00 8000.00 0.00 0.05550 16800.00 8000.00 0.00 0.05855 17000.00 8000.00 0.00 0.06170 17200.00 8000.00 0.00 0.06491 17400.00 8000.00 0.00 0.06811 17600.00 8000.00 0.00 0.07132 17800.00 8000.00 0.00 0.07457 18000.00 8000.00 0.00 0.07789 18200.00 8000.00 0.00 0.08128 18400.00 8000.00 0.00 0.08465 18600.00 8000.00 0.00 0.08783 18800.00 8000.00 0.00 0.09062 19000.00 8000.00 0.00 0.09282 19200.00 8000.00 0.00 0.09428 19400.00 8000.00 0.00 0.09499 19600.00 8000.00 0.00 0.09508 19200.00 8200.00 0.00 0.09531 19400.00 8200.00 0.00 0.09597 19600.00 8200.00 0.00 0.09602 19200.00 8400.00 0.00 0.09629 19400.00 8400.00 0.00 0.09692 19600.00 8400.00 0.00 0.09694 19200.00 8600.00 0.00 0.09743 19400.00 8600.00 0.00 0.09804 19600.00 8600.00 0.00 0.09805 19200.00 8800.00 0.00 0.09881 19400.00 8800.00 0.00 0.09939 19600.00 8800.00 0.00 0.09941 19200.00 9000.00 0.00 0.10034 19400.00 9000.00 0.00 0.10092 19600.00 9000.00 0.00 0.10094 19200.00 9200.00 0.00 0.10199 19400.00 9200.00 0.00 0.10257 19600.00 9200.00 0.00 0.10259 19200.00 9400.00 0.00 0.10369 19400.00 9400.00 0.00 0.10426 19600.00 9400.00 0.00 0.10429 19200.00 9600.00 0.00 0.10539 19400.00 9600.00 0.00 0.10595 19600.00 9600.00 0.00 0.10598 19200.00 9800.00 0.00 0.10716 19400.00 9800.00 0.00 0.10771 19600.00 9800.00 0.00 0.10775 19000.00 10000.00 0.00 0.10778 19200.00 10000.00 0.00 0.10903 19400.00 10000.00 0.00 0.10958 19600.00 10000.00 0.00 0.10962 19000.00 10200.00 0.00 0.10964 19200.00 10200.00 0.00 0.11087 19400.00 10200.00 0.00 0.11142 19600.00 10200.00 0.00 0.11146 19200.00 10400.00 0.00 0.11263 19400.00 10400.00 0.00 0.11316 19600.00 10400.00 0.00 0.11318 19200.00 10600.00 0.00 0.11427 19400.00 10600.00 0.00 0.11478 19200.00 10800.00 0.00 0.11580 19400.00 10800.00 0.00 0.11629 19200.00 11000.00 0.00 0.11740 19400.00 11000.00 0.00 0.11786 19000.00 11200.00 0.00 0.11810 19200.00 11200.00 0.00 0.11924 19400.00 11200.00 0.00 0.11967 19000.00 11400.00 0.00 0.12016 19200.00 11400.00 0.00 0.12128 19400.00 11400.00 0.00 0.12168 19000.00 11600.00 0.00 0.12234 19200.00 11600.00 0.00 0.12342 19400.00 11600.00 0.00 0.12379 19000.00 11800.00 0.00 0.12456 19200.00 11800.00 0.00 0.12560 19400.00 11800.00 0.00 0.12594 19000.00 12000.00 0.00 0.12672 19200.00 12000.00 0.00 0.12772 19400.00 12000.00 0.00 0.12802 19000.00 12200.00 0.00 0.12886 19200.00 12200.00 0.00 0.12983 19400.00 12200.00 0.00 0.13011 19000.00 12400.00 0.00 0.13120 19200.00 12400.00 0.00 0.13214 19400.00 12400.00 0.00 0.13240 19000.00 12600.00 0.00 0.13376 19200.00 12600.00 0.00 0.13467 19400.00 12600.00 0.00 0.13491 19000.00 12800.00 0.00 0.13643 19200.00 12800.00 0.00 0.13731 19400.00 12800.00 0.00 0.13752 19000.00 13000.00 0.00 0.13916 19200.00 13000.00 0.00 0.14002 19400.00 13000.00 0.00 0.14022 19000.00 13200.00 0.00 0.14187 19200.00 13200.00 0.00 0.14272 19400.00 13200.00 0.00 0.14293 19000.00 13400.00 0.00 0.14455 19200.00 13400.00 0.00 0.14540 19400.00 13400.00 0.00 0.14561 18800.00 13600.00 0.00 0.14573 19000.00 13600.00 0.00 0.14738 19200.00 13600.00 0.00 0.14823 19400.00 13600.00 0.00 0.14844 18800.00 13800.00 0.00 0.14881 19000.00 13800.00 0.00 0.15045 19200.00 13800.00 0.00 0.15129 19400.00 13800.00 0.00 0.15149 18800.00 14000.00 0.00 0.15197 19000.00 14000.00 0.00 0.15360 19200.00 14000.00 0.00 0.15442 19400.00 14000.00 0.00 0.15460 18800.00 14200.00 0.00 0.15514 19000.00 14200.00 0.00 0.15674 19200.00 14200.00 0.00 0.15754 19400.00 14200.00 0.00 0.15768 18800.00 14400.00 0.00 0.15823 19000.00 14400.00 0.00 0.15981 19200.00 14400.00 0.00 0.16057 19400.00 14400.00 0.00 0.16068 18800.00 14600.00 0.00 0.16113 19000.00 14600.00 0.00 0.16268 19200.00 14600.00 0.00 0.16340 19400.00 14600.00 0.00 0.16346 18800.00 14800.00 0.00 0.16396 19000.00 14800.00 0.00 0.16548 19200.00 14800.00 0.00 0.16617 19400.00 14800.00 0.00 0.16618 18800.00 15000.00 0.00 0.16697 19000.00 15000.00 0.00 0.16847 19200.00 15000.00 0.00 0.16913 18800.00 15200.00 0.00 0.17015 19000.00 15200.00 0.00 0.17163 19200.00 15200.00 0.00 0.17227 18800.00 15400.00 0.00 0.17342 19000.00 15400.00 0.00 0.17490 19200.00 15400.00 0.00 0.17551 18800.00 15600.00 0.00 0.17684 19000.00 15600.00 0.00 0.17831 19200.00 15600.00 0.00 0.17890 18800.00 15800.00 0.00 0.18035 19000.00 15800.00 0.00 0.18182 19200.00 15800.00 0.00 0.18235 18800.00 16000.00 0.00 0.18397 19000.00 16000.00 0.00 0.18539 19200.00 16000.00 0.00 0.18584 18800.00 16200.00 0.00 0.18786 19000.00 16200.00 0.00 0.18920 19200.00 16200.00 0.00 0.18952 18600.00 16400.00 0.00 0.18970 18800.00 16400.00 0.00 0.19195 19000.00 16400.00 0.00 0.19316 19200.00 16400.00 0.00 0.19332 18600.00 16600.00 0.00 0.19377 18800.00 16600.00 0.00 0.19590 19000.00 16600.00 0.00 0.19695 18600.00 16800.00 0.00 0.19757 18800.00 16800.00 0.00 0.19958 19000.00 16800.00 0.00 0.20047 18600.00 17000.00 0.00 0.20104 18800.00 17000.00 0.00 0.20292 19000.00 17000.00 0.00 0.20366 18600.00 17200.00 0.00 0.20424 18800.00 17200.00 0.00 0.20601 19000.00 17200.00 0.00 0.20659 18600.00 17400.00 0.00 0.20764 18800.00 17400.00 0.00 0.20926 19000.00 17400.00 0.00 0.20964 18600.00 17600.00 0.00 0.21155 18800.00 17600.00 0.00 0.21292 19000.00 17600.00 0.00 0.21303 18400.00 17800.00 0.00 0.21306 18600.00 17800.00 0.00 0.21563 18800.00 17800.00 0.00 0.21665 18400.00 18000.00 0.00 0.21729 18600.00 18000.00 0.00 0.21945 18800.00 18000.00 0.00 0.22004 18400.00 18200.00 0.00 0.22099 18600.00 18200.00 0.00 0.22261 18800.00 18200.00 0.00 0.22268 18400.00 18400.00 0.00 0.22343 18600.00 18400.00 0.00 0.22444 18600.00 18600.00 0.00 0.22488 18600.00 18800.00 20.00 0.22490 18400.00 18800.00 25.00 0.22492 18600.00 18800.00 25.00 0.22495 18400.00 19000.00 25.00 0.22497 18600.00 18800.00 30.00 0.22498 18400.00 19000.00 30.00 0.22504 18400.00 19000.00 35.00 0.22506 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 18484.56 18886.59 34.84 0.22524 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/229.ctf NX, NY, NZ: 360 360 1 MODE : real